Objective: To evaluate the anti-tumor effects of SeO2 and its mechanisms on three human lung cancer cell lines. Methods: Three lung cancer cells A549, GLC-82 and PG were treated with 3-30 μmol/L SeO2. Flow cytometry ...Objective: To evaluate the anti-tumor effects of SeO2 and its mechanisms on three human lung cancer cell lines. Methods: Three lung cancer cells A549, GLC-82 and PG were treated with 3-30 μmol/L SeO2. Flow cytometry was used to detect apoptosis, and analyze the changes of expression of p53 and Bcl-2, as well as ROS and Ca2+ level within cells. Results:SeO2 markedly inhibited cell proliferation and viability, and prompted apoptosis after 48 h treatment. SeO2 at 10 μmol/L induced 47.8% apoptosis in A549 cells, 40.8% in GLC-82 cells, 18.2% in PG cells. SeO2 at 30 μmol/L induced 37.8% apoposis in PG cells,but did not increase apoptotic raes in other two cells. SeO2 could down-regulate the mean fluorescent intensity of Bcl-2 from 65.8 to 9.6 in A549, but not in GLC-82 and in PG cells, up-regulate wild type p53 level in all three cells. SeO2 decreased the ROS and Ca2+ level markedly within three tested cells. Conclusion: SeO2 showed anti-tumor effect via apoptosis pathway in three lung cancer cell lines. The decrease of ROS and Ca2+ level within cells as well as regulation of Bcl-2 and p53 expression may play important roles in above apoptotic procedure.展开更多
To discover novel fluoro p hores of solution and solid dual-state emission(DSE) materials,unique V-shape furo[2,3-b]furans have been designed and synthesized by a one-pot method for the first time and their photolumin...To discover novel fluoro p hores of solution and solid dual-state emission(DSE) materials,unique V-shape furo[2,3-b]furans have been designed and synthesized by a one-pot method for the first time and their photoluminescent properties have been explored in benzene,THF,DMF and DMSO,as well as in the solid state.As the best example,2,5-bis(4-(9 H-carbazol-9-yl)phenyl)-6 a-amino-3 a,6 a-dihydrofuro[2,3-b]furan-3,3 a,4-trica rbonitrile(3 g) exhibited solution and solid DSE properties in THF,benzene,and in the solid state with quantum yields of 55%,92%,and 45%,respectively.展开更多
1-[1-（Benzyloxy）-3-methylnaphthalen-4-yloxy]propan-2-one （la） took place a cyclization, debenzylation and oxidation to form 9-substitued benzo[de]chromene-7,8-dione （2a） and 5-benzyl-9-substitued benzo[de]chrome...1-[1-（Benzyloxy）-3-methylnaphthalen-4-yloxy]propan-2-one （la） took place a cyclization, debenzylation and oxidation to form 9-substitued benzo[de]chromene-7,8-dione （2a） and 5-benzyl-9-substitued benzo[de]chromene-7,8-dione （3a）. The mechanisms for these reactions were discussed.展开更多
基金This project was partially supported by Science Foundation of Lanzhou Command of PLA(No.YZ-0106).
文摘Objective: To evaluate the anti-tumor effects of SeO2 and its mechanisms on three human lung cancer cell lines. Methods: Three lung cancer cells A549, GLC-82 and PG were treated with 3-30 μmol/L SeO2. Flow cytometry was used to detect apoptosis, and analyze the changes of expression of p53 and Bcl-2, as well as ROS and Ca2+ level within cells. Results:SeO2 markedly inhibited cell proliferation and viability, and prompted apoptosis after 48 h treatment. SeO2 at 10 μmol/L induced 47.8% apoptosis in A549 cells, 40.8% in GLC-82 cells, 18.2% in PG cells. SeO2 at 30 μmol/L induced 37.8% apoposis in PG cells,but did not increase apoptotic raes in other two cells. SeO2 could down-regulate the mean fluorescent intensity of Bcl-2 from 65.8 to 9.6 in A549, but not in GLC-82 and in PG cells, up-regulate wild type p53 level in all three cells. SeO2 decreased the ROS and Ca2+ level markedly within three tested cells. Conclusion: SeO2 showed anti-tumor effect via apoptosis pathway in three lung cancer cell lines. The decrease of ROS and Ca2+ level within cells as well as regulation of Bcl-2 and p53 expression may play important roles in above apoptotic procedure.
基金supported by grants from the National Natural Science Foundation of China(No.21702239)Guangzhou Science and Technology Plan Projects(No.201707010271)the Fundamental Research Funds for the Central Universities(No.16lgpy16)。
文摘To discover novel fluoro p hores of solution and solid dual-state emission(DSE) materials,unique V-shape furo[2,3-b]furans have been designed and synthesized by a one-pot method for the first time and their photoluminescent properties have been explored in benzene,THF,DMF and DMSO,as well as in the solid state.As the best example,2,5-bis(4-(9 H-carbazol-9-yl)phenyl)-6 a-amino-3 a,6 a-dihydrofuro[2,3-b]furan-3,3 a,4-trica rbonitrile(3 g) exhibited solution and solid DSE properties in THF,benzene,and in the solid state with quantum yields of 55%,92%,and 45%,respectively.
基金We are indebted to the National Natural Science Foundation of China （No.20272085,20472117）the Guangzhou City Science Foundation,the Guangdong Provincial Science Foundation（031594）the Scientific Research Foundation for the Returned 0verseas Chinese Scholars,and the HongKong Polytechnic University Area of Strategic Development Fund for financial support of this study.
文摘1-[1-（Benzyloxy）-3-methylnaphthalen-4-yloxy]propan-2-one （la） took place a cyclization, debenzylation and oxidation to form 9-substitued benzo[de]chromene-7,8-dione （2a） and 5-benzyl-9-substitued benzo[de]chromene-7,8-dione （3a）. The mechanisms for these reactions were discussed.
