BACKGROUND： It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution...BACKGROUND： It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE： To explore a semi-automatic computerized three-dimensional （3D） reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING： An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING： Department of Anatomy, Institute of Neurobiology, Jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS： A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS： After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at x25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with ＂Curves＂, ＂Autocontrast＂, ＂Gray scale 8 bit＂, ＂Invert＂, ＂Image resize to 50%＂ steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a personal computer. The reconstructed human lumbar spinal cord segment was interactively observed, cut, and measured. MAIN OUTCOME MEASURES： The reconstructed human lumbar spinal cord segment. RESULTS： Compared with serial images obtained from other image modalities, such as CT, MRI, and macrosections from The Visual Human Project database, the Luxol Fast Blue stained histological serial section images exhibited higher resolution and contrast between gray and white matter. Image processing and 3D reconstruction steps were semi-automatically performed with related software. The 3D reconstructed human lumbar cord segment were observed, cut, and measured on a PC. CONCLUSION： A semi-automatically computerized method, based on histological serial sections, is an effective way to 3D-reconstruct the human spinal cord.展开更多
A new method for reconstructing a 3-dimensional object from serial cross-sectionsis presented in this paper.The method is based on the principle of sampling and considersevery point in cross-sections as a sampling poi...A new method for reconstructing a 3-dimensional object from serial cross-sectionsis presented in this paper.The method is based on the principle of sampling and considersevery point in cross-sections as a sampling point and performs the interpolating of nonlinearfunction with these sampling points.Compared with other methods,this method has manyadvantages such as higher precision and fewer requested known sampling points.The result ofreconstruction with this method is an“entity”which involves the exterior shape and interiorconstruction information of the object simultaneously.展开更多
The mechanical properties of rocks under cyclic and dynamic loading are important research topics for solving the structural stability of large engineering rocks. As underground mining in coal mines goes deeper, groun...The mechanical properties of rocks under cyclic and dynamic loading are important research topics for solving the structural stability of large engineering rocks. As underground mining in coal mines goes deeper, ground stresses are increasing and instability damage of coal rocks by shear loading is frequent. Therefore, in order to investigate the shear mechanical properties and section morphological characteristics of intact coal samples in the direct shear test, the RDS-200 rock direct shear instrument was used to carry out direct shear tests on intact coal samples under different normal stresses, and the shear section was scanned for three-dimensional morphology. The results show that: 1) from the strength characteristics, the peak shear strength of the coal samples increased linearly with increasing normal stress, and the residual shear strength increased logarithmically. 2) In terms of deformation characteristics, the peak shear displacement of the coal sample increases linearly with increasing normal stress, the pre-peak shear stiffness increases logarithmically, and the residual normal displacement decreases linearly. 3) From the morphological characteristics of the shear surface, with the increase of normal stress, the section transitions from high-order undulating to flattening type. The maximum height of the fracture surface profile and kurtosis coefficient of the shear section decreased linearly, and the profile area ratio and root mean square of slope decreased as a power function, i.e. the higher the normal stress, the smaller the undulation of the section, the sharpness of the roughness shape and the roughness coefficient JRC, and the flatter and smoother the section. The findings of this study can help to provide some reference for the evaluation of shear instability occurring in coal bodies under different normal stresses.展开更多
For the section coupled system of multilayer dynamics of fluids in porous media, a parallel scheme modified by the characteristic finite difference fractional steps is proposed for a complete point set consisting of c...For the section coupled system of multilayer dynamics of fluids in porous media, a parallel scheme modified by the characteristic finite difference fractional steps is proposed for a complete point set consisting of coarse and fine partitions. Some tech- niques, such as calculus of variations, energy method, twofold-quadratic interpolation of product type, multiplicative commutation law of difference operators, decomposition of high order difference operators, and prior estimates, are used in theoretical analysis. Optimal order estimates in 12 norm are derived to show accuracy of the second order approximation solutions. These methods have been used to simulate the problems of migration-accumulation of oil resources.展开更多
基金Natural Science Research Plan forJiangsu Colleges, No.05KJB180105 Postgraduate Innovation Cultivating Projectin Jiangsu Province, No.CX07s_035z
文摘BACKGROUND： It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE： To explore a semi-automatic computerized three-dimensional （3D） reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING： An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING： Department of Anatomy, Institute of Neurobiology, Jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS： A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS： After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at x25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with ＂Curves＂, ＂Autocontrast＂, ＂Gray scale 8 bit＂, ＂Invert＂, ＂Image resize to 50%＂ steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a personal computer. The reconstructed human lumbar spinal cord segment was interactively observed, cut, and measured. MAIN OUTCOME MEASURES： The reconstructed human lumbar spinal cord segment. RESULTS： Compared with serial images obtained from other image modalities, such as CT, MRI, and macrosections from The Visual Human Project database, the Luxol Fast Blue stained histological serial section images exhibited higher resolution and contrast between gray and white matter. Image processing and 3D reconstruction steps were semi-automatically performed with related software. The 3D reconstructed human lumbar cord segment were observed, cut, and measured on a PC. CONCLUSION： A semi-automatically computerized method, based on histological serial sections, is an effective way to 3D-reconstruct the human spinal cord.
文摘A new method for reconstructing a 3-dimensional object from serial cross-sectionsis presented in this paper.The method is based on the principle of sampling and considersevery point in cross-sections as a sampling point and performs the interpolating of nonlinearfunction with these sampling points.Compared with other methods,this method has manyadvantages such as higher precision and fewer requested known sampling points.The result ofreconstruction with this method is an“entity”which involves the exterior shape and interiorconstruction information of the object simultaneously.
文摘The mechanical properties of rocks under cyclic and dynamic loading are important research topics for solving the structural stability of large engineering rocks. As underground mining in coal mines goes deeper, ground stresses are increasing and instability damage of coal rocks by shear loading is frequent. Therefore, in order to investigate the shear mechanical properties and section morphological characteristics of intact coal samples in the direct shear test, the RDS-200 rock direct shear instrument was used to carry out direct shear tests on intact coal samples under different normal stresses, and the shear section was scanned for three-dimensional morphology. The results show that: 1) from the strength characteristics, the peak shear strength of the coal samples increased linearly with increasing normal stress, and the residual shear strength increased logarithmically. 2) In terms of deformation characteristics, the peak shear displacement of the coal sample increases linearly with increasing normal stress, the pre-peak shear stiffness increases logarithmically, and the residual normal displacement decreases linearly. 3) From the morphological characteristics of the shear surface, with the increase of normal stress, the section transitions from high-order undulating to flattening type. The maximum height of the fracture surface profile and kurtosis coefficient of the shear section decreased linearly, and the profile area ratio and root mean square of slope decreased as a power function, i.e. the higher the normal stress, the smaller the undulation of the section, the sharpness of the roughness shape and the roughness coefficient JRC, and the flatter and smoother the section. The findings of this study can help to provide some reference for the evaluation of shear instability occurring in coal bodies under different normal stresses.
基金supported by the Major State Basic Research Program of China(No.19990328)the National Tackling Key Program(No.20050200069)+1 种基金the National Natural Science Foundation of China(Nos.10372052,10771124,11101244,and 11271231)the Doctorate Foundation of the State Education Commission(No.20030422047)
文摘For the section coupled system of multilayer dynamics of fluids in porous media, a parallel scheme modified by the characteristic finite difference fractional steps is proposed for a complete point set consisting of coarse and fine partitions. Some tech- niques, such as calculus of variations, energy method, twofold-quadratic interpolation of product type, multiplicative commutation law of difference operators, decomposition of high order difference operators, and prior estimates, are used in theoretical analysis. Optimal order estimates in 12 norm are derived to show accuracy of the second order approximation solutions. These methods have been used to simulate the problems of migration-accumulation of oil resources.
