Silver carp, Hypophthalmichthys molitrix, and bighead carp, Aristichthys nobilis, habitually celebrated for their being cultured easily with fewer diseases and high-yield, have suffered from a new severe epidemic dise...Silver carp, Hypophthalmichthys molitrix, and bighead carp, Aristichthys nobilis, habitually celebrated for their being cultured easily with fewer diseases and high-yield, have suffered from a new severe epidemic disease recently. The spread of the epizootic has been far and wide, from several provinces in South China to the Beijing and Tianjin area. The affected fish suffered heavy mortafity in one week, and even died off.展开更多
During the present investigation,studies were carried out to investigate the outbreak of Redmouth disease in culture ponds situated at Krishna,West Godavari and East Godavari districts of Andhra Pradesh.Enteric Red Mo...During the present investigation,studies were carried out to investigate the outbreak of Redmouth disease in culture ponds situated at Krishna,West Godavari and East Godavari districts of Andhra Pradesh.Enteric Red Mouth disease or Yersiniosis is a serious infectious bacterial disease,sometimes responsible for causing severe economic loss to fish farming industry.The disease is clinically characterized by symptoms like bleeding at the base of fins,inside and around mouth,bilateral exophthalmia with or without haemorrhage,and per-ocular and peri-oral haemorrhages.In the present study,live or moribund fish showing lesions similar to enteric redmouth disease were collected and the causative agent was isolated by culture techniques.Bacterial identification was carried out by conventional biochemical methods and molecular polymerase chain reaction.DNA extracted from isolated pathogen was subjected to PCR amplification with a pair of Y.ruckeri specific primers.The PCR product was run on 1.5%agarose gel electrophoresis and positive bands with a molecular size of approximately 500bp were detected.It is similar to that given for PCR products with primers specific for Y.ruckeri.展开更多
为鉴定鱼源鲁氏耶尔森氏菌(Yersinia ruckeri)SC09菌株水生环境中不同温度的转录组水平上的差异,研究采用链特异性转录组测序(Strand-specific RNA-seq)技术对菌体生理温度(28℃)和实验培养温度(37℃)下进行链特异性测序,原始数据质控后...为鉴定鱼源鲁氏耶尔森氏菌(Yersinia ruckeri)SC09菌株水生环境中不同温度的转录组水平上的差异,研究采用链特异性转录组测序(Strand-specific RNA-seq)技术对菌体生理温度(28℃)和实验培养温度(37℃)下进行链特异性测序,原始数据质控后,筛选得到差异表达基因,通过KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库对差异表达基因进行富集分析,并利用Rockhopper软件筛选出的重要原核生物基因簇进行验证。结果显示,共筛选获得173个显著差异表达基因(P<0.05),其中包括58个上调基因,主要富集到一些特殊的碳水化合物代谢相关的通路中;以及115个下调基因,主要富集到双组份信号系统中与三羧酸循环相关的代谢通路上,同时部分基因富集到编码鞭毛素相关的基因簇中。结果表明,相对于37℃的实验室培养温度,在水生环境的生理温度条件下(28℃)SC09菌株拥有较高的运动性和较强的葡萄糖代谢,但相对的SC09菌株代谢一些特殊糖类的能力减弱。展开更多
【目的】确定草鱼(Ctenopharyngodon idellus)中出现的以肌肉出血、脾脏肿大、肠炎、鳔出血为典型症状的疾病的病原,为该病的防治提供参考。【方法】采用病原分离纯化、生理生化试验、16S r DNA检测、系统进化分析、动物回归感染试验等...【目的】确定草鱼(Ctenopharyngodon idellus)中出现的以肌肉出血、脾脏肿大、肠炎、鳔出血为典型症状的疾病的病原,为该病的防治提供参考。【方法】采用病原分离纯化、生理生化试验、16S r DNA检测、系统进化分析、动物回归感染试验等方法对致病菌进行鉴定,采用纸片法确定病原的药敏特性。【结果】分离株为革兰氏阴性短杆菌,菌落表面光滑、圆形,颜色为白色;有运动性,硫化氢反应、V-P反应阴性,能发酵葡萄糖、果糖、麦芽糖;16S r DNA测序结果与鲁氏耶尔森菌(Yersinia ruckeri)同源性为99%;回归试验攻毒组与临床病例表现一致;药敏试验结果显示该菌对氨苄西林、恩诺沙星、氟苯尼考等19种抗菌药敏感,对青霉素G、苯唑西林、利福平、万古霉素、克拉霉素、红霉素、头孢他啶、麦迪霉素和痢特灵存在耐药性。【结论】该例草鱼疾病的病原为鲁氏耶尔森菌,在草鱼上为首次报道,生产中可选用恩诺沙星、氟苯尼考等水产类常用抗菌药进行防治。展开更多
The present study examined the influence of leucine metabolite β-hydroxy-β-methylbutyrate （HMB） on the nonspecific cellular and humoral defence mechanisms and protection against Yersinia ruckeri and Aeromonas salm...The present study examined the influence of leucine metabolite β-hydroxy-β-methylbutyrate （HMB） on the nonspecific cellular and humoral defence mechanisms and protection against Yersinia ruckeri and Aeromonas salmonicida in juvenile pikeperch （Sander lucioperca）. The fish was fed with a commercial trout pellets, containing either 0 （control group） or 50 mg HMB kg^-1 body weight day^-1 （HMB fed group）. After four weeks feeding, 20 pikeperch from each group were anaesthetised and blood and pronephros samples were taken. The levels of the following immunological parameters were measured： respiratory burst activity of phagocytes, potential killing activity of phagocytes, lymphocytes proliferation stimulated by concanavaline A or lipopolisaccharide, lysozyme activity in serum, ceruloplasmin activity in serum, total protein in serum and total immunoglobulin （Ig） level in serum. A disease challenge test using Yersinia ruckeri or Aeromonas salmonicida was conducted after 4 weeks of feeding. The levels of all immunological parameters excluding ceruloplasmin activity and total protein in serum were statistically significantly higher （P 〈 0.05） in the HMB treated group compared to the control group. Feeding with HMB has led to a significant decrease in the mortality after the challenge trial with Y. ruckeri （30% lower mortality than in the control group） and with theA. salmonicida （40% lower mortality than in the control group）. The current study strongly suggests that feeding pikeperch with HMB in dose 50 mg kg^-1 body weight day^-1 may improve the innate immunity and decrease the mortality rates after experimental infection with pathogenic bacteria Y. ruckeri and A. salmonicida.展开更多
文摘Silver carp, Hypophthalmichthys molitrix, and bighead carp, Aristichthys nobilis, habitually celebrated for their being cultured easily with fewer diseases and high-yield, have suffered from a new severe epidemic disease recently. The spread of the epizootic has been far and wide, from several provinces in South China to the Beijing and Tianjin area. The affected fish suffered heavy mortafity in one week, and even died off.
基金The financial support was provided by a fellowship Grant from“Maulana Azad National Fellowship Programme”New Delhi,India.
文摘During the present investigation,studies were carried out to investigate the outbreak of Redmouth disease in culture ponds situated at Krishna,West Godavari and East Godavari districts of Andhra Pradesh.Enteric Red Mouth disease or Yersiniosis is a serious infectious bacterial disease,sometimes responsible for causing severe economic loss to fish farming industry.The disease is clinically characterized by symptoms like bleeding at the base of fins,inside and around mouth,bilateral exophthalmia with or without haemorrhage,and per-ocular and peri-oral haemorrhages.In the present study,live or moribund fish showing lesions similar to enteric redmouth disease were collected and the causative agent was isolated by culture techniques.Bacterial identification was carried out by conventional biochemical methods and molecular polymerase chain reaction.DNA extracted from isolated pathogen was subjected to PCR amplification with a pair of Y.ruckeri specific primers.The PCR product was run on 1.5%agarose gel electrophoresis and positive bands with a molecular size of approximately 500bp were detected.It is similar to that given for PCR products with primers specific for Y.ruckeri.
文摘为鉴定鱼源鲁氏耶尔森氏菌(Yersinia ruckeri)SC09菌株水生环境中不同温度的转录组水平上的差异,研究采用链特异性转录组测序(Strand-specific RNA-seq)技术对菌体生理温度(28℃)和实验培养温度(37℃)下进行链特异性测序,原始数据质控后,筛选得到差异表达基因,通过KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库对差异表达基因进行富集分析,并利用Rockhopper软件筛选出的重要原核生物基因簇进行验证。结果显示,共筛选获得173个显著差异表达基因(P<0.05),其中包括58个上调基因,主要富集到一些特殊的碳水化合物代谢相关的通路中;以及115个下调基因,主要富集到双组份信号系统中与三羧酸循环相关的代谢通路上,同时部分基因富集到编码鞭毛素相关的基因簇中。结果表明,相对于37℃的实验室培养温度,在水生环境的生理温度条件下(28℃)SC09菌株拥有较高的运动性和较强的葡萄糖代谢,但相对的SC09菌株代谢一些特殊糖类的能力减弱。
文摘【目的】确定草鱼(Ctenopharyngodon idellus)中出现的以肌肉出血、脾脏肿大、肠炎、鳔出血为典型症状的疾病的病原,为该病的防治提供参考。【方法】采用病原分离纯化、生理生化试验、16S r DNA检测、系统进化分析、动物回归感染试验等方法对致病菌进行鉴定,采用纸片法确定病原的药敏特性。【结果】分离株为革兰氏阴性短杆菌,菌落表面光滑、圆形,颜色为白色;有运动性,硫化氢反应、V-P反应阴性,能发酵葡萄糖、果糖、麦芽糖;16S r DNA测序结果与鲁氏耶尔森菌(Yersinia ruckeri)同源性为99%;回归试验攻毒组与临床病例表现一致;药敏试验结果显示该菌对氨苄西林、恩诺沙星、氟苯尼考等19种抗菌药敏感,对青霉素G、苯唑西林、利福平、万古霉素、克拉霉素、红霉素、头孢他啶、麦迪霉素和痢特灵存在耐药性。【结论】该例草鱼疾病的病原为鲁氏耶尔森菌,在草鱼上为首次报道,生产中可选用恩诺沙星、氟苯尼考等水产类常用抗菌药进行防治。
文摘The present study examined the influence of leucine metabolite β-hydroxy-β-methylbutyrate （HMB） on the nonspecific cellular and humoral defence mechanisms and protection against Yersinia ruckeri and Aeromonas salmonicida in juvenile pikeperch （Sander lucioperca）. The fish was fed with a commercial trout pellets, containing either 0 （control group） or 50 mg HMB kg^-1 body weight day^-1 （HMB fed group）. After four weeks feeding, 20 pikeperch from each group were anaesthetised and blood and pronephros samples were taken. The levels of the following immunological parameters were measured： respiratory burst activity of phagocytes, potential killing activity of phagocytes, lymphocytes proliferation stimulated by concanavaline A or lipopolisaccharide, lysozyme activity in serum, ceruloplasmin activity in serum, total protein in serum and total immunoglobulin （Ig） level in serum. A disease challenge test using Yersinia ruckeri or Aeromonas salmonicida was conducted after 4 weeks of feeding. The levels of all immunological parameters excluding ceruloplasmin activity and total protein in serum were statistically significantly higher （P 〈 0.05） in the HMB treated group compared to the control group. Feeding with HMB has led to a significant decrease in the mortality after the challenge trial with Y. ruckeri （30% lower mortality than in the control group） and with theA. salmonicida （40% lower mortality than in the control group）. The current study strongly suggests that feeding pikeperch with HMB in dose 50 mg kg^-1 body weight day^-1 may improve the innate immunity and decrease the mortality rates after experimental infection with pathogenic bacteria Y. ruckeri and A. salmonicida.
