Scientists have discovered that root-dwelling bacteria directly control rice tillering-a crucial yield trait-by producing a compound that mimics plant hormones.The study,published in Cell on June 12,2025,reveals how m...Scientists have discovered that root-dwelling bacteria directly control rice tillering-a crucial yield trait-by producing a compound that mimics plant hormones.The study,published in Cell on June 12,2025,reveals how microbes partner with crops to shape agricultural productivity.展开更多
Plant microbiota can intimately interact with their hosts and can strongly influence agronomic traits.They can alter phenotypes independent of genotype in field conditions.For example,they can interfere in the synthes...Plant microbiota can intimately interact with their hosts and can strongly influence agronomic traits.They can alter phenotypes independent of genotype in field conditions.For example,they can interfere in the synthesis,perception,or downstream signaling of phytohormones,such as auxin,cytokinin,ethylene,abscisic acid,and gibberellin(Egamberdieva et al.,2017).Recently,Zhang et al.(2025)have shown in their recent paper how genera of root microbiota can increase or decrease rice tiller number by interacting with the strigolactone(SL)pathway,which inhibits rice tillering.展开更多
背景:近年来,生物陶瓷材料因具有良好的生物相容性和封闭性能逐渐成为根尖倒充填的首选材料,其中生物陶瓷材料C-Root BP、iRoot BP Plus均具有良好的生物相容性和封闭性能。目的:对比体外环境下C-Root BP与iRoot BP Plus材料的根尖封闭...背景:近年来,生物陶瓷材料因具有良好的生物相容性和封闭性能逐渐成为根尖倒充填的首选材料,其中生物陶瓷材料C-Root BP、iRoot BP Plus均具有良好的生物相容性和封闭性能。目的:对比体外环境下C-Root BP与iRoot BP Plus材料的根尖封闭性能及抗力强度。方法:于2022年6月至2024年6月期间,收集石家庄市第二医院口腔科因正畸或牙周病新鲜拔除的单根离体牙56颗,随机分4组处理:iRoot BP Plus组(n=16)、C-Root BP组(n=16)根管预备和消毒后分别采用iRoot BP Plus、C-Root BP材料进行根尖倒充填,阳性对照组(n=16)根管预备和消毒后使用蒸馏水进行根尖倒充填,阴性对照组(n=8)仅行根管预备和消毒(不进行根尖倒充填处理),采用染料渗透法检测根尖封闭性;制作根管细菌渗漏体外模型,评价细菌微渗漏发生情况;采用薄片推出实验检测填充材料与牙本质之间的粘连强度,并在显微镜下观察断裂形式。结果与结论:(1)染料染色7 d后,阳性对照组染料渗透长度大于iRoot BP Plus组、C-Root BP组(P<0.05),iRoot BP Plus组和C-Root BP组染料渗透长度比较差异无显著性意义(P>0.05);(2)经过90 d培养,阳性对照组细菌微渗漏发生率高于iRoot BP Plus组、C-Root BP组(P<0.05),iRoot BPPlus组和C-Root BP组细菌微渗漏发生率比较差异无显著性意义(P>0.05);(3)C-Root BP组填充材料与牙本质之间的粘连强度大于iRootBP Plus组(P<0.05),两组断裂面断裂形式比较差异无显著性意义(P>0.05);(4)结果表明,采用C-Root BP和iRoot BP Plus材料进行根尖倒充填可产生相似的根尖封闭效果,并且断裂形式也基本一致,但C-Root BP材料具有良好的粘连强度。展开更多
The freshwater luminescent bacteria Vibrio-qinghaiensis sp.-Q67 test and the Vicia faba root tip test associated with solid-phase extraction were applied for cytotoxicity and genotoxicity assessment of organic substan...The freshwater luminescent bacteria Vibrio-qinghaiensis sp.-Q67 test and the Vicia faba root tip test associated with solid-phase extraction were applied for cytotoxicity and genotoxicity assessment of organic substances in three rivers, two lakes and effluent flows from two wastewater treatment plants （WWTPs） in Xi＇an, China. Although the most seriously polluted fiver with high chemical oxygen demand （COD） and total organic carbon （TOC） showed high cytotoxicity （expressed as TIIs0, the toxicity impact index） and genotoxicity （expressed as RMCN, the relative frequency of micronucleus）, no correlative relation was found between the ecotoxicity and organic content of the water samples. However, there was a linear correlative relation between TIIs0 and RMCN for most water samples except that from the Zaohe River, which receives discharge from WWTP and untreated industrial wastewaters. The ecotoxicity of the organic toxicants in the Chanhe River and Zaohe River indicated that cytotoxic and genotoxic effects were related to the pollutant source. The TII50 and RMCN were also found to correlate roughly to the dissolved oxygen concentration of the water. Sufficient dissolved oxygen in surface water is thus proved to be an indicator of a healthy water environmental condition.展开更多
Plant-associated bacteria that inhabit the rhizosphere may influence the plant growth by their contribution to the endogenous pool of phytohormones and by the activity of ACC deaminase to decrease the ethylene concent...Plant-associated bacteria that inhabit the rhizosphere may influence the plant growth by their contribution to the endogenous pool of phytohormones and by the activity of ACC deaminase to decrease the ethylene concentration. The aim of this study was to analyse the root length growth by the promoting effect of indole acetic acid producers phytobacteria with ACC deaminase activity, on inoculated seeds of Lens esculenta as synergistic effect on root elongation. In this study, although the roots of L. esculenta seedlings do not show a significant promotion, these phytobacteria could be recommended to treat plants analyzing their added inoculum to increase plant biomass and retard the effect of ethylene on cultures supplied with Tryptophan and ACC.展开更多
The aim of the study was to assess the effects of various biopreparations on the growth of the strawberry root system, the number of spores of mycorrhizal fungi, the total number of bacteria in the rhizosphere soil, a...The aim of the study was to assess the effects of various biopreparations on the growth of the strawberry root system, the number of spores of mycorrhizal fungi, the total number of bacteria in the rhizosphere soil, and the degree of mycorrhizal association in the roots of two strawberry cultivars. The experiment with strawberry plants was established in the spring of 2010 in the Experimental Orchard of the Institute of Horticulture in Dabrowice. The objects of research were “frigo” strawberry plants of the cultivars Elsanta and Elkat. The following experimental combinations were used: control, control NPK (standard NPK fertilization), manure, mycorrhizal preparation Micosat F, Humus UP, Humus Active + Aktywit PM, BioFeed Amin, BioFeed Quality, Tytanit, Vinassa, Florovit Eko, and Florovit Pro Natura. The use of the biopreparation BioFeed Quality resulted in a six-fold increase in root length and a seven-fold increase in root surface area. Compared with NPK fertilization, application of the preparation BioFeed Amin contributed to an eight-fold increase in root volume, and the use of Vinassa increased 24-fold the number of root tips of Elkat strawberry plants. Micosat F and Humus UP caused a five-fold increase in mycorrhizal frequency in the roots of strawberry plants. Micosat F and manure contributed to a two- and four-fold increase, respectively, in the number of spores in the rhizosphere soil. Application of the preparations Humus UP, BioFeed Amin and Florovit Eko doubled the total number of bacteria and filamentous fungi in the rhizosphere soil of strawberry plants of the cultivars Elsanta and Elkat in comparison with NPK fertilization. Fertilization with the biopreparations intensified the growth of the root system and increased the number of spores of AM fungi, mycorrhizal frequency, and the total number of bacteria and filamentous fungi in the soil.展开更多
Plant growth promontory Pseudomonas strains were isolated from root nodules of five plant species, viz., Trifolium pretense, Cicer arietinum, Amaranthus polygamus, Vigna mungo, and Trigonella foenum;that plants were d...Plant growth promontory Pseudomonas strains were isolated from root nodules of five plant species, viz., Trifolium pretense, Cicer arietinum, Amaranthus polygamus, Vigna mungo, and Trigonella foenum;that plants were denizen of Shekhawati region of Rajasthan. A total of 8 bacterial isolates were evaluated for growth promotion using PGP properties. Partial 16S rDNA sequencing data showed that these 8 bacterial isolates belonged to genus Pseudomonas. MEGA 4.0.2, software was used to construct a neighbor joining tree by employing boot strap method. Result exhibited significant diversity among recovered Pseudomonas strains.展开更多
Purpose: The aim of this systematic review was to answer the question “Which treatment protocol, am- ong classical methods and/or various laser applica- tions is the most effective in root canal disinfection, in vitr...Purpose: The aim of this systematic review was to answer the question “Which treatment protocol, am- ong classical methods and/or various laser applica- tions is the most effective in root canal disinfection, in vitro”. Materials and Methods: A MEDLINE, a Co- chrane and an Embase search (three specified search- es) were conducted to identify randomized controlled trials (RCT) until June 2010, conducted on human teeth and published in English, German or French language, examining the root canal disinfection after the use of lasers with or without mechanical instru-mentation. Additionally, hand search was conducted and contact with authors, when needed. Results: The MEDLINE, the Cochrane and the EMBASE search identified 240, 28, and 35 published articles, respec-tively. Ten articles from the MEDLINE and 5 articles from the Cochrane search (that were also identified in the MEDLINE search) met the inclusion and va-lidity assessment criteria. In E. faecalis elimination, instrumentation of the root canal and diode laser/665 nanometer/1 Watt (diode laser/665 nm/1 W) irradia-tion with the combined effect of Methylene Blue (MB) as photosensitizing agent (logCFU/ml = 1636) seemed to be the best method. In P. aeruginosa and in A.naeslundii elimination, instrumentation of the root canal followed by irrigation with 5, 25% NaOCl (log-CFU/ml = 0) seemed to be the best method. In gen-eral, instrumentation of the root canal followed by irrigation with 5, 25% NaOCl (logCFU/ml = 0) and instrumentation of the root canal and Er: YAG laser/ 2940 nm/0.8 W irradiation (logCFU/ml = 1924) seemed to be the best (polymicrobial studies). Conclusions: There are treatment protocols with the assistance or not of laser irradiation that can eliminate E. faecalis, E. coli and S. aureus inside the root canal. However, there is a serious number of S. anginosus, F. nuclea-tum, A. naeslundii and P. aeruginosa that remain in-side the root canal even after laser irradiation. New research is needed in order to set a treatment proto-col effective in the root canal disinfection from all bac-teria that are related to endodontic origin pathology.展开更多
文摘Scientists have discovered that root-dwelling bacteria directly control rice tillering-a crucial yield trait-by producing a compound that mimics plant hormones.The study,published in Cell on June 12,2025,reveals how microbes partner with crops to shape agricultural productivity.
文摘Plant microbiota can intimately interact with their hosts and can strongly influence agronomic traits.They can alter phenotypes independent of genotype in field conditions.For example,they can interfere in the synthesis,perception,or downstream signaling of phytohormones,such as auxin,cytokinin,ethylene,abscisic acid,and gibberellin(Egamberdieva et al.,2017).Recently,Zhang et al.(2025)have shown in their recent paper how genera of root microbiota can increase or decrease rice tiller number by interacting with the strigolactone(SL)pathway,which inhibits rice tillering.
文摘背景:近年来,生物陶瓷材料因具有良好的生物相容性和封闭性能逐渐成为根尖倒充填的首选材料,其中生物陶瓷材料C-Root BP、iRoot BP Plus均具有良好的生物相容性和封闭性能。目的:对比体外环境下C-Root BP与iRoot BP Plus材料的根尖封闭性能及抗力强度。方法:于2022年6月至2024年6月期间,收集石家庄市第二医院口腔科因正畸或牙周病新鲜拔除的单根离体牙56颗,随机分4组处理:iRoot BP Plus组(n=16)、C-Root BP组(n=16)根管预备和消毒后分别采用iRoot BP Plus、C-Root BP材料进行根尖倒充填,阳性对照组(n=16)根管预备和消毒后使用蒸馏水进行根尖倒充填,阴性对照组(n=8)仅行根管预备和消毒(不进行根尖倒充填处理),采用染料渗透法检测根尖封闭性;制作根管细菌渗漏体外模型,评价细菌微渗漏发生情况;采用薄片推出实验检测填充材料与牙本质之间的粘连强度,并在显微镜下观察断裂形式。结果与结论:(1)染料染色7 d后,阳性对照组染料渗透长度大于iRoot BP Plus组、C-Root BP组(P<0.05),iRoot BP Plus组和C-Root BP组染料渗透长度比较差异无显著性意义(P>0.05);(2)经过90 d培养,阳性对照组细菌微渗漏发生率高于iRoot BP Plus组、C-Root BP组(P<0.05),iRoot BPPlus组和C-Root BP组细菌微渗漏发生率比较差异无显著性意义(P>0.05);(3)C-Root BP组填充材料与牙本质之间的粘连强度大于iRootBP Plus组(P<0.05),两组断裂面断裂形式比较差异无显著性意义(P>0.05);(4)结果表明,采用C-Root BP和iRoot BP Plus材料进行根尖倒充填可产生相似的根尖封闭效果,并且断裂形式也基本一致,但C-Root BP材料具有良好的粘连强度。
基金supported by the National Natural Science Foundation of China (No. 50838005)the Program for Changjiang Scholars and Innovative Research Team inUniversity (No. IRT0853)the National Program of Water Pollution Control (No. 2008ZX07317-004)
文摘The freshwater luminescent bacteria Vibrio-qinghaiensis sp.-Q67 test and the Vicia faba root tip test associated with solid-phase extraction were applied for cytotoxicity and genotoxicity assessment of organic substances in three rivers, two lakes and effluent flows from two wastewater treatment plants （WWTPs） in Xi＇an, China. Although the most seriously polluted fiver with high chemical oxygen demand （COD） and total organic carbon （TOC） showed high cytotoxicity （expressed as TIIs0, the toxicity impact index） and genotoxicity （expressed as RMCN, the relative frequency of micronucleus）, no correlative relation was found between the ecotoxicity and organic content of the water samples. However, there was a linear correlative relation between TIIs0 and RMCN for most water samples except that from the Zaohe River, which receives discharge from WWTP and untreated industrial wastewaters. The ecotoxicity of the organic toxicants in the Chanhe River and Zaohe River indicated that cytotoxic and genotoxic effects were related to the pollutant source. The TII50 and RMCN were also found to correlate roughly to the dissolved oxygen concentration of the water. Sufficient dissolved oxygen in surface water is thus proved to be an indicator of a healthy water environmental condition.
基金Authors are grateful to the Research Projects:SIP:20131494 of the Secretaría de Investigación y Posgrado del I.P.N.ISITDF/325/11 AREAS PRIORITARIAS-IPN and COFAA-IPN,EDI-IPN,SNI-CONACYT fel-lowships
文摘Plant-associated bacteria that inhabit the rhizosphere may influence the plant growth by their contribution to the endogenous pool of phytohormones and by the activity of ACC deaminase to decrease the ethylene concentration. The aim of this study was to analyse the root length growth by the promoting effect of indole acetic acid producers phytobacteria with ACC deaminase activity, on inoculated seeds of Lens esculenta as synergistic effect on root elongation. In this study, although the roots of L. esculenta seedlings do not show a significant promotion, these phytobacteria could be recommended to treat plants analyzing their added inoculum to increase plant biomass and retard the effect of ethylene on cultures supplied with Tryptophan and ACC.
文摘The aim of the study was to assess the effects of various biopreparations on the growth of the strawberry root system, the number of spores of mycorrhizal fungi, the total number of bacteria in the rhizosphere soil, and the degree of mycorrhizal association in the roots of two strawberry cultivars. The experiment with strawberry plants was established in the spring of 2010 in the Experimental Orchard of the Institute of Horticulture in Dabrowice. The objects of research were “frigo” strawberry plants of the cultivars Elsanta and Elkat. The following experimental combinations were used: control, control NPK (standard NPK fertilization), manure, mycorrhizal preparation Micosat F, Humus UP, Humus Active + Aktywit PM, BioFeed Amin, BioFeed Quality, Tytanit, Vinassa, Florovit Eko, and Florovit Pro Natura. The use of the biopreparation BioFeed Quality resulted in a six-fold increase in root length and a seven-fold increase in root surface area. Compared with NPK fertilization, application of the preparation BioFeed Amin contributed to an eight-fold increase in root volume, and the use of Vinassa increased 24-fold the number of root tips of Elkat strawberry plants. Micosat F and Humus UP caused a five-fold increase in mycorrhizal frequency in the roots of strawberry plants. Micosat F and manure contributed to a two- and four-fold increase, respectively, in the number of spores in the rhizosphere soil. Application of the preparations Humus UP, BioFeed Amin and Florovit Eko doubled the total number of bacteria and filamentous fungi in the rhizosphere soil of strawberry plants of the cultivars Elsanta and Elkat in comparison with NPK fertilization. Fertilization with the biopreparations intensified the growth of the root system and increased the number of spores of AM fungi, mycorrhizal frequency, and the total number of bacteria and filamentous fungi in the soil.
文摘Plant growth promontory Pseudomonas strains were isolated from root nodules of five plant species, viz., Trifolium pretense, Cicer arietinum, Amaranthus polygamus, Vigna mungo, and Trigonella foenum;that plants were denizen of Shekhawati region of Rajasthan. A total of 8 bacterial isolates were evaluated for growth promotion using PGP properties. Partial 16S rDNA sequencing data showed that these 8 bacterial isolates belonged to genus Pseudomonas. MEGA 4.0.2, software was used to construct a neighbor joining tree by employing boot strap method. Result exhibited significant diversity among recovered Pseudomonas strains.
文摘Purpose: The aim of this systematic review was to answer the question “Which treatment protocol, am- ong classical methods and/or various laser applica- tions is the most effective in root canal disinfection, in vitro”. Materials and Methods: A MEDLINE, a Co- chrane and an Embase search (three specified search- es) were conducted to identify randomized controlled trials (RCT) until June 2010, conducted on human teeth and published in English, German or French language, examining the root canal disinfection after the use of lasers with or without mechanical instru-mentation. Additionally, hand search was conducted and contact with authors, when needed. Results: The MEDLINE, the Cochrane and the EMBASE search identified 240, 28, and 35 published articles, respec-tively. Ten articles from the MEDLINE and 5 articles from the Cochrane search (that were also identified in the MEDLINE search) met the inclusion and va-lidity assessment criteria. In E. faecalis elimination, instrumentation of the root canal and diode laser/665 nanometer/1 Watt (diode laser/665 nm/1 W) irradia-tion with the combined effect of Methylene Blue (MB) as photosensitizing agent (logCFU/ml = 1636) seemed to be the best method. In P. aeruginosa and in A.naeslundii elimination, instrumentation of the root canal followed by irrigation with 5, 25% NaOCl (log-CFU/ml = 0) seemed to be the best method. In gen-eral, instrumentation of the root canal followed by irrigation with 5, 25% NaOCl (logCFU/ml = 0) and instrumentation of the root canal and Er: YAG laser/ 2940 nm/0.8 W irradiation (logCFU/ml = 1924) seemed to be the best (polymicrobial studies). Conclusions: There are treatment protocols with the assistance or not of laser irradiation that can eliminate E. faecalis, E. coli and S. aureus inside the root canal. However, there is a serious number of S. anginosus, F. nuclea-tum, A. naeslundii and P. aeruginosa that remain in-side the root canal even after laser irradiation. New research is needed in order to set a treatment proto-col effective in the root canal disinfection from all bac-teria that are related to endodontic origin pathology.
