Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered specie...Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered species.While significant technological breakthroughs have been achieved in cooling methods-particularly through vitrification for large tissue and organs-the lack of optimal rewarming technology remains a key obstacle to successful cryopreservation,especially for larger samples such as tissues and organs.The primary challenges during the warming process include non-uniformity heating and insufficient rewarming rates,which can lead to thermal stress-induced structural damage and lethal ice recrystallization,ultimately compromising the integrity and functionality of biological materials.In recent years,various advanced warming techniques have emerged,employing different energy conversion approaches to achieve volumetric heating while minimizing the risk of overheating.These techniques involve thermal,mechanical-thermal,and electromagnetic-thermal energy conversions.However,each method presents its own limitation.This review aims to summarize recent advancements in rewarming technologies for cryopreservation,with a focus on their mechanisms,applications,and the key challenges that must be addressed to enable broader adoption in medical and commercial contexts.展开更多
目的探究急诊创伤患者低体温防治的最佳证据,为急诊创伤患者低体温的科学管理提供依据。方法计算机检索PubMed、Embase、Web of Science、BMJ Best Practice、UpToDate、Cochrane Library、英国国家卫生与临床优化研究所网站、Joanna Br...目的探究急诊创伤患者低体温防治的最佳证据,为急诊创伤患者低体温的科学管理提供依据。方法计算机检索PubMed、Embase、Web of Science、BMJ Best Practice、UpToDate、Cochrane Library、英国国家卫生与临床优化研究所网站、Joanna Briggs Institute(JBI)循证卫生保健中心及万方等数据库中与创伤患者低体温相关的文献。检索时限为建库至2024年12月31日。对符合质量标准的文献,采用相应的证据分级与推荐级别系统进行证据提取和等级划分。结果共纳入7篇文献,包括临床指南3篇、文献综述3篇及系统评价1篇。最终形成16条最佳证据,分为一级预防(针对未发生低体温的患者,预防其发生)和二级预防(针对已发生低体温的患者,控制病情发展及预防并发症)。一级预防包括体温测量、低体温识别、低体温评估及低体温预防4个方面;二级预防包括体温管理目标、体温监测频率及体温复温措施3个方面。证据推荐强度中,A级推荐13条、B级推荐3条。结论急诊创伤患者入院后需快速测量体核温度(鼓膜或腔内)以确认是否低体温(<36℃),并依据分级评估。应通过调高室温、常规保暖预防低体温。根据分级选择监测频率,采取复温措施维持体核温度在36℃~37℃,为急诊创伤患者低体温的防治提供参考和借鉴。展开更多
为研究梯度回温对低温贮藏荔枝货架期品质保持的作用,以“八月红”荔枝为实验材料,经过1 d 1℃冷库贮藏后,依次经过9、18和25℃三个温度环境后逐渐回到室温(25℃),设为“梯度回温”处理组,同时设置1 d 1℃冷库贮藏后直接放入25℃环境恢...为研究梯度回温对低温贮藏荔枝货架期品质保持的作用,以“八月红”荔枝为实验材料,经过1 d 1℃冷库贮藏后,依次经过9、18和25℃三个温度环境后逐渐回到室温(25℃),设为“梯度回温”处理组,同时设置1 d 1℃冷库贮藏后直接放入25℃环境恢复室温(25℃)的荔枝为“直接回温”处理组作为对照,分别模拟货架在常温放置0、1、3、5 d,并对货架期荔枝果实各项指标进行测定。结果表明,“梯度回温”处理能够缓解荔枝褐变,回温处理后在常温放置5 d时,“直接回温”荔枝的褐变指数为1.50,“梯度回温”荔枝的褐变指数仅为1.00。此外检测发现“梯度回温”延缓了可溶性固形物、可滴定酸、总酚、类黄酮、花青素含量的减少,并有效抑制了果皮中多酚氧化酶和过氧化物酶活性的增加。因此,采用梯度回温方式处理能有效缓解低温贮藏荔枝品质劣变,延长货架保鲜期,具有广阔的应用前景。展开更多
Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted r...Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted reproduction and the cryopreservation of sperm,eggs and embryos,as well as the preservation of skin,fingers,and other isolated tissues.However,cryopreservation of large and complex tissues or organs remains highly challenging.In addition to the damage caused by the freezing and rewarming processes and the inherent complexity of tissues and organs,there is an urgent need to address issues related to damage detection and the investigation of injury mechanisms.It provides a retrospective analysis of existing methods for assessing tissue and organ viability.Although current techniques can detect damage to some extent,they tend to be relatively simple,time-consuming,and limited in their ability to provide timely and comprehensive assessments of viability.By summarizing and evaluating these approaches,our study aims to contribute to the improvement of viability detection methods and to promote further development in this critical area.展开更多
基金supported by National Natural Science Foundation of China(Award Number:2346842).
文摘Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered species.While significant technological breakthroughs have been achieved in cooling methods-particularly through vitrification for large tissue and organs-the lack of optimal rewarming technology remains a key obstacle to successful cryopreservation,especially for larger samples such as tissues and organs.The primary challenges during the warming process include non-uniformity heating and insufficient rewarming rates,which can lead to thermal stress-induced structural damage and lethal ice recrystallization,ultimately compromising the integrity and functionality of biological materials.In recent years,various advanced warming techniques have emerged,employing different energy conversion approaches to achieve volumetric heating while minimizing the risk of overheating.These techniques involve thermal,mechanical-thermal,and electromagnetic-thermal energy conversions.However,each method presents its own limitation.This review aims to summarize recent advancements in rewarming technologies for cryopreservation,with a focus on their mechanisms,applications,and the key challenges that must be addressed to enable broader adoption in medical and commercial contexts.
文摘目的探究急诊创伤患者低体温防治的最佳证据,为急诊创伤患者低体温的科学管理提供依据。方法计算机检索PubMed、Embase、Web of Science、BMJ Best Practice、UpToDate、Cochrane Library、英国国家卫生与临床优化研究所网站、Joanna Briggs Institute(JBI)循证卫生保健中心及万方等数据库中与创伤患者低体温相关的文献。检索时限为建库至2024年12月31日。对符合质量标准的文献,采用相应的证据分级与推荐级别系统进行证据提取和等级划分。结果共纳入7篇文献,包括临床指南3篇、文献综述3篇及系统评价1篇。最终形成16条最佳证据,分为一级预防(针对未发生低体温的患者,预防其发生)和二级预防(针对已发生低体温的患者,控制病情发展及预防并发症)。一级预防包括体温测量、低体温识别、低体温评估及低体温预防4个方面;二级预防包括体温管理目标、体温监测频率及体温复温措施3个方面。证据推荐强度中,A级推荐13条、B级推荐3条。结论急诊创伤患者入院后需快速测量体核温度(鼓膜或腔内)以确认是否低体温(<36℃),并依据分级评估。应通过调高室温、常规保暖预防低体温。根据分级选择监测频率,采取复温措施维持体核温度在36℃~37℃,为急诊创伤患者低体温的防治提供参考和借鉴。
文摘为研究梯度回温对低温贮藏荔枝货架期品质保持的作用,以“八月红”荔枝为实验材料,经过1 d 1℃冷库贮藏后,依次经过9、18和25℃三个温度环境后逐渐回到室温(25℃),设为“梯度回温”处理组,同时设置1 d 1℃冷库贮藏后直接放入25℃环境恢复室温(25℃)的荔枝为“直接回温”处理组作为对照,分别模拟货架在常温放置0、1、3、5 d,并对货架期荔枝果实各项指标进行测定。结果表明,“梯度回温”处理能够缓解荔枝褐变,回温处理后在常温放置5 d时,“直接回温”荔枝的褐变指数为1.50,“梯度回温”荔枝的褐变指数仅为1.00。此外检测发现“梯度回温”延缓了可溶性固形物、可滴定酸、总酚、类黄酮、花青素含量的减少,并有效抑制了果皮中多酚氧化酶和过氧化物酶活性的增加。因此,采用梯度回温方式处理能有效缓解低温贮藏荔枝品质劣变,延长货架保鲜期,具有广阔的应用前景。
文摘Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted reproduction and the cryopreservation of sperm,eggs and embryos,as well as the preservation of skin,fingers,and other isolated tissues.However,cryopreservation of large and complex tissues or organs remains highly challenging.In addition to the damage caused by the freezing and rewarming processes and the inherent complexity of tissues and organs,there is an urgent need to address issues related to damage detection and the investigation of injury mechanisms.It provides a retrospective analysis of existing methods for assessing tissue and organ viability.Although current techniques can detect damage to some extent,they tend to be relatively simple,time-consuming,and limited in their ability to provide timely and comprehensive assessments of viability.By summarizing and evaluating these approaches,our study aims to contribute to the improvement of viability detection methods and to promote further development in this critical area.
