Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered specie...Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered species.While significant technological breakthroughs have been achieved in cooling methods-particularly through vitrification for large tissue and organs-the lack of optimal rewarming technology remains a key obstacle to successful cryopreservation,especially for larger samples such as tissues and organs.The primary challenges during the warming process include non-uniformity heating and insufficient rewarming rates,which can lead to thermal stress-induced structural damage and lethal ice recrystallization,ultimately compromising the integrity and functionality of biological materials.In recent years,various advanced warming techniques have emerged,employing different energy conversion approaches to achieve volumetric heating while minimizing the risk of overheating.These techniques involve thermal,mechanical-thermal,and electromagnetic-thermal energy conversions.However,each method presents its own limitation.This review aims to summarize recent advancements in rewarming technologies for cryopreservation,with a focus on their mechanisms,applications,and the key challenges that must be addressed to enable broader adoption in medical and commercial contexts.展开更多
Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted r...Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted reproduction and the cryopreservation of sperm,eggs and embryos,as well as the preservation of skin,fingers,and other isolated tissues.However,cryopreservation of large and complex tissues or organs remains highly challenging.In addition to the damage caused by the freezing and rewarming processes and the inherent complexity of tissues and organs,there is an urgent need to address issues related to damage detection and the investigation of injury mechanisms.It provides a retrospective analysis of existing methods for assessing tissue and organ viability.Although current techniques can detect damage to some extent,they tend to be relatively simple,time-consuming,and limited in their ability to provide timely and comprehensive assessments of viability.By summarizing and evaluating these approaches,our study aims to contribute to the improvement of viability detection methods and to promote further development in this critical area.展开更多
为研究梯度回温对低温贮藏荔枝货架期品质保持的作用,以“八月红”荔枝为实验材料,经过1 d 1℃冷库贮藏后,依次经过9、18和25℃三个温度环境后逐渐回到室温(25℃),设为“梯度回温”处理组,同时设置1 d 1℃冷库贮藏后直接放入25℃环境恢...为研究梯度回温对低温贮藏荔枝货架期品质保持的作用,以“八月红”荔枝为实验材料,经过1 d 1℃冷库贮藏后,依次经过9、18和25℃三个温度环境后逐渐回到室温(25℃),设为“梯度回温”处理组,同时设置1 d 1℃冷库贮藏后直接放入25℃环境恢复室温(25℃)的荔枝为“直接回温”处理组作为对照,分别模拟货架在常温放置0、1、3、5 d,并对货架期荔枝果实各项指标进行测定。结果表明,“梯度回温”处理能够缓解荔枝褐变,回温处理后在常温放置5 d时,“直接回温”荔枝的褐变指数为1.50,“梯度回温”荔枝的褐变指数仅为1.00。此外检测发现“梯度回温”延缓了可溶性固形物、可滴定酸、总酚、类黄酮、花青素含量的减少,并有效抑制了果皮中多酚氧化酶和过氧化物酶活性的增加。因此,采用梯度回温方式处理能有效缓解低温贮藏荔枝品质劣变,延长货架保鲜期,具有广阔的应用前景。展开更多
目的 探讨预防性加温输液在急诊严重创伤患者中的应用效果。方法 选取2022年1月至12月上海市第六人民医院收治的急诊严重创伤患者84例,采用随机数字表法分为对照组(42例)和观察组(42例)。对照组采用常规干预和常规保温措施,观察组在对...目的 探讨预防性加温输液在急诊严重创伤患者中的应用效果。方法 选取2022年1月至12月上海市第六人民医院收治的急诊严重创伤患者84例,采用随机数字表法分为对照组(42例)和观察组(42例)。对照组采用常规干预和常规保温措施,观察组在对照组的基础上进行预防性加温输液。比较两组体温值、凝血指标和酸碱指标变化,28 d存活率,寒战、低体温发生率。结果 两组体温组间、时间点及交互作用比较,差异有统计学意义(P<0.05)。进一步两两比较,组内比较:两组组内各时间点体温值比较,差异无统计学意义(P>0.05);组间比较:观察组入院后4、8、12、24 h体温值高于对照组,差异有统计学意义(P<0.05)。两组TT水平组间、时间点及交互作用比较,差异有统计学意义(P<0.05)。其中,两组入院后24 h TT水平高于入院时,观察组入院6、12、24 h TT水平低于对照组,差异有统计学意义(P<0.05)。两组不同时间FIB水平比较,差异有统计学意义(P<0.05)。其中两组入院后24 h FIB水平低于入院时,差异有统计学意义(P<0.05)。两组不同时间点血乳酸水平比较,差异有统计学意义(P<0.05)。其中两组入院后6、12、24 h血乳酸水平高于入院时,差异有统计学意义(P<0.05)。两组p H值水平组间、时间点比较,差异有统计学意义(P<0.05)。其中两组入院后6、12、24 h p H值水平低于入院时,差异有统计学意义(P<0.05)。观察组寒战、低体温发生率低于对照组,差异有统计学意义(P<0.05)。两组28 d存活率比较,差异无统计学意义(P>0.05)。结论 预防性加温输液可减少寒战和低体温的发生,并改善患者凝血功能。展开更多
【目的】青田田鱼在浅水环境中经常面临急性低温胁迫,探究青田田鱼在急性低温和复温环境下的生理响应机制,为青田田鱼科学越冬和耐低温品种选育提供理论依据。【方法】通过液相色谱—质谱(LC-MS)非靶向代谢组学技术,提取9.0℃胁迫6 h(CO...【目的】青田田鱼在浅水环境中经常面临急性低温胁迫,探究青田田鱼在急性低温和复温环境下的生理响应机制,为青田田鱼科学越冬和耐低温品种选育提供理论依据。【方法】通过液相色谱—质谱(LC-MS)非靶向代谢组学技术,提取9.0℃胁迫6 h(CO组)、升温至28.0℃复温恢复6 h(RE组)和28.0℃对照组(Con组)青田田鱼鳃组织样本代谢峰。预处理后的代谢组数据与数据库匹配鉴定代谢物种类。通过主成分分析(PCA)和正交最小偏二乘判别分析(OPLS-DA)鉴定代谢组学数据的可靠性。根据Student’s test检验的P值(P<0.05)和OPLS-DA模型得到的变量权重值(VIP>1)筛选组间差异代谢物。对差异代谢物进行KEGG信号通路富集分析,并用Fisher精确检验分析筛选与急性低温和复温最相关的代谢通路。对重要代谢通路制作聚类热图,显示组间差异和代谢物水平变化趋势。【结果】PCA和OPLS-DA分析结果显示,组内样本聚集,组间样本分离,组间的代谢物水平存在显著差异。OPLS-DA得分图显示各组Q2累计值均在0.5以上,R2Y累积值均接近1.0,模型具有较高的解释度和可靠性;共鉴定到1222个代谢物,其中CO vs Con,RE vs CO和RE vs Con分别筛选鉴定出232、238和300种显著差异代谢物(P<0.05)。各组间差异代谢物主要富集在花生四烯酸代谢、甘油磷脂代谢、类固醇激素生物合成和嘌呤代谢等代谢途径;急性低温胁迫下,皮质醇水平上调,而花生四烯酸及其代谢物和甘油磷脂代谢物下调。复温后花生四烯酸、甘油磷脂代谢物和嘌呤代谢物相对Con组下调,皮质醇含量下调,牛磺酸含量上调。【结论】急性低温可导致青田田鱼免疫、物质转运和信号传递等功能异常。皮质醇的合成和代谢可能是青田田鱼抵抗急性低温胁迫的重要机制。复温后青田田鱼鳃组织损伤在短期内无法得到完全恢复。牛磺酸合成可能是青田田鱼在复温过程中的重要恢复机制。展开更多
基金supported by National Natural Science Foundation of China(Award Number:2346842).
文摘Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered species.While significant technological breakthroughs have been achieved in cooling methods-particularly through vitrification for large tissue and organs-the lack of optimal rewarming technology remains a key obstacle to successful cryopreservation,especially for larger samples such as tissues and organs.The primary challenges during the warming process include non-uniformity heating and insufficient rewarming rates,which can lead to thermal stress-induced structural damage and lethal ice recrystallization,ultimately compromising the integrity and functionality of biological materials.In recent years,various advanced warming techniques have emerged,employing different energy conversion approaches to achieve volumetric heating while minimizing the risk of overheating.These techniques involve thermal,mechanical-thermal,and electromagnetic-thermal energy conversions.However,each method presents its own limitation.This review aims to summarize recent advancements in rewarming technologies for cryopreservation,with a focus on their mechanisms,applications,and the key challenges that must be addressed to enable broader adoption in medical and commercial contexts.
文摘Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted reproduction and the cryopreservation of sperm,eggs and embryos,as well as the preservation of skin,fingers,and other isolated tissues.However,cryopreservation of large and complex tissues or organs remains highly challenging.In addition to the damage caused by the freezing and rewarming processes and the inherent complexity of tissues and organs,there is an urgent need to address issues related to damage detection and the investigation of injury mechanisms.It provides a retrospective analysis of existing methods for assessing tissue and organ viability.Although current techniques can detect damage to some extent,they tend to be relatively simple,time-consuming,and limited in their ability to provide timely and comprehensive assessments of viability.By summarizing and evaluating these approaches,our study aims to contribute to the improvement of viability detection methods and to promote further development in this critical area.
文摘为研究梯度回温对低温贮藏荔枝货架期品质保持的作用,以“八月红”荔枝为实验材料,经过1 d 1℃冷库贮藏后,依次经过9、18和25℃三个温度环境后逐渐回到室温(25℃),设为“梯度回温”处理组,同时设置1 d 1℃冷库贮藏后直接放入25℃环境恢复室温(25℃)的荔枝为“直接回温”处理组作为对照,分别模拟货架在常温放置0、1、3、5 d,并对货架期荔枝果实各项指标进行测定。结果表明,“梯度回温”处理能够缓解荔枝褐变,回温处理后在常温放置5 d时,“直接回温”荔枝的褐变指数为1.50,“梯度回温”荔枝的褐变指数仅为1.00。此外检测发现“梯度回温”延缓了可溶性固形物、可滴定酸、总酚、类黄酮、花青素含量的减少,并有效抑制了果皮中多酚氧化酶和过氧化物酶活性的增加。因此,采用梯度回温方式处理能有效缓解低温贮藏荔枝品质劣变,延长货架保鲜期,具有广阔的应用前景。
文摘目的 探讨预防性加温输液在急诊严重创伤患者中的应用效果。方法 选取2022年1月至12月上海市第六人民医院收治的急诊严重创伤患者84例,采用随机数字表法分为对照组(42例)和观察组(42例)。对照组采用常规干预和常规保温措施,观察组在对照组的基础上进行预防性加温输液。比较两组体温值、凝血指标和酸碱指标变化,28 d存活率,寒战、低体温发生率。结果 两组体温组间、时间点及交互作用比较,差异有统计学意义(P<0.05)。进一步两两比较,组内比较:两组组内各时间点体温值比较,差异无统计学意义(P>0.05);组间比较:观察组入院后4、8、12、24 h体温值高于对照组,差异有统计学意义(P<0.05)。两组TT水平组间、时间点及交互作用比较,差异有统计学意义(P<0.05)。其中,两组入院后24 h TT水平高于入院时,观察组入院6、12、24 h TT水平低于对照组,差异有统计学意义(P<0.05)。两组不同时间FIB水平比较,差异有统计学意义(P<0.05)。其中两组入院后24 h FIB水平低于入院时,差异有统计学意义(P<0.05)。两组不同时间点血乳酸水平比较,差异有统计学意义(P<0.05)。其中两组入院后6、12、24 h血乳酸水平高于入院时,差异有统计学意义(P<0.05)。两组p H值水平组间、时间点比较,差异有统计学意义(P<0.05)。其中两组入院后6、12、24 h p H值水平低于入院时,差异有统计学意义(P<0.05)。观察组寒战、低体温发生率低于对照组,差异有统计学意义(P<0.05)。两组28 d存活率比较,差异无统计学意义(P>0.05)。结论 预防性加温输液可减少寒战和低体温的发生,并改善患者凝血功能。
文摘【目的】青田田鱼在浅水环境中经常面临急性低温胁迫,探究青田田鱼在急性低温和复温环境下的生理响应机制,为青田田鱼科学越冬和耐低温品种选育提供理论依据。【方法】通过液相色谱—质谱(LC-MS)非靶向代谢组学技术,提取9.0℃胁迫6 h(CO组)、升温至28.0℃复温恢复6 h(RE组)和28.0℃对照组(Con组)青田田鱼鳃组织样本代谢峰。预处理后的代谢组数据与数据库匹配鉴定代谢物种类。通过主成分分析(PCA)和正交最小偏二乘判别分析(OPLS-DA)鉴定代谢组学数据的可靠性。根据Student’s test检验的P值(P<0.05)和OPLS-DA模型得到的变量权重值(VIP>1)筛选组间差异代谢物。对差异代谢物进行KEGG信号通路富集分析,并用Fisher精确检验分析筛选与急性低温和复温最相关的代谢通路。对重要代谢通路制作聚类热图,显示组间差异和代谢物水平变化趋势。【结果】PCA和OPLS-DA分析结果显示,组内样本聚集,组间样本分离,组间的代谢物水平存在显著差异。OPLS-DA得分图显示各组Q2累计值均在0.5以上,R2Y累积值均接近1.0,模型具有较高的解释度和可靠性;共鉴定到1222个代谢物,其中CO vs Con,RE vs CO和RE vs Con分别筛选鉴定出232、238和300种显著差异代谢物(P<0.05)。各组间差异代谢物主要富集在花生四烯酸代谢、甘油磷脂代谢、类固醇激素生物合成和嘌呤代谢等代谢途径;急性低温胁迫下,皮质醇水平上调,而花生四烯酸及其代谢物和甘油磷脂代谢物下调。复温后花生四烯酸、甘油磷脂代谢物和嘌呤代谢物相对Con组下调,皮质醇含量下调,牛磺酸含量上调。【结论】急性低温可导致青田田鱼免疫、物质转运和信号传递等功能异常。皮质醇的合成和代谢可能是青田田鱼抵抗急性低温胁迫的重要机制。复温后青田田鱼鳃组织损伤在短期内无法得到完全恢复。牛磺酸合成可能是青田田鱼在复温过程中的重要恢复机制。
