Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered specie...Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered species.While significant technological breakthroughs have been achieved in cooling methods-particularly through vitrification for large tissue and organs-the lack of optimal rewarming technology remains a key obstacle to successful cryopreservation,especially for larger samples such as tissues and organs.The primary challenges during the warming process include non-uniformity heating and insufficient rewarming rates,which can lead to thermal stress-induced structural damage and lethal ice recrystallization,ultimately compromising the integrity and functionality of biological materials.In recent years,various advanced warming techniques have emerged,employing different energy conversion approaches to achieve volumetric heating while minimizing the risk of overheating.These techniques involve thermal,mechanical-thermal,and electromagnetic-thermal energy conversions.However,each method presents its own limitation.This review aims to summarize recent advancements in rewarming technologies for cryopreservation,with a focus on their mechanisms,applications,and the key challenges that must be addressed to enable broader adoption in medical and commercial contexts.展开更多
Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted r...Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted reproduction and the cryopreservation of sperm,eggs and embryos,as well as the preservation of skin,fingers,and other isolated tissues.However,cryopreservation of large and complex tissues or organs remains highly challenging.In addition to the damage caused by the freezing and rewarming processes and the inherent complexity of tissues and organs,there is an urgent need to address issues related to damage detection and the investigation of injury mechanisms.It provides a retrospective analysis of existing methods for assessing tissue and organ viability.Although current techniques can detect damage to some extent,they tend to be relatively simple,time-consuming,and limited in their ability to provide timely and comprehensive assessments of viability.By summarizing and evaluating these approaches,our study aims to contribute to the improvement of viability detection methods and to promote further development in this critical area.展开更多
为研究梯度回温对低温贮藏荔枝货架期品质保持的作用,以“八月红”荔枝为实验材料,经过1 d 1℃冷库贮藏后,依次经过9、18和25℃三个温度环境后逐渐回到室温(25℃),设为“梯度回温”处理组,同时设置1 d 1℃冷库贮藏后直接放入25℃环境恢...为研究梯度回温对低温贮藏荔枝货架期品质保持的作用,以“八月红”荔枝为实验材料,经过1 d 1℃冷库贮藏后,依次经过9、18和25℃三个温度环境后逐渐回到室温(25℃),设为“梯度回温”处理组,同时设置1 d 1℃冷库贮藏后直接放入25℃环境恢复室温(25℃)的荔枝为“直接回温”处理组作为对照,分别模拟货架在常温放置0、1、3、5 d,并对货架期荔枝果实各项指标进行测定。结果表明,“梯度回温”处理能够缓解荔枝褐变,回温处理后在常温放置5 d时,“直接回温”荔枝的褐变指数为1.50,“梯度回温”荔枝的褐变指数仅为1.00。此外检测发现“梯度回温”延缓了可溶性固形物、可滴定酸、总酚、类黄酮、花青素含量的减少,并有效抑制了果皮中多酚氧化酶和过氧化物酶活性的增加。因此,采用梯度回温方式处理能有效缓解低温贮藏荔枝品质劣变,延长货架保鲜期,具有广阔的应用前景。展开更多
基金supported by National Natural Science Foundation of China(Award Number:2346842).
文摘Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered species.While significant technological breakthroughs have been achieved in cooling methods-particularly through vitrification for large tissue and organs-the lack of optimal rewarming technology remains a key obstacle to successful cryopreservation,especially for larger samples such as tissues and organs.The primary challenges during the warming process include non-uniformity heating and insufficient rewarming rates,which can lead to thermal stress-induced structural damage and lethal ice recrystallization,ultimately compromising the integrity and functionality of biological materials.In recent years,various advanced warming techniques have emerged,employing different energy conversion approaches to achieve volumetric heating while minimizing the risk of overheating.These techniques involve thermal,mechanical-thermal,and electromagnetic-thermal energy conversions.However,each method presents its own limitation.This review aims to summarize recent advancements in rewarming technologies for cryopreservation,with a focus on their mechanisms,applications,and the key challenges that must be addressed to enable broader adoption in medical and commercial contexts.
文摘Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted reproduction and the cryopreservation of sperm,eggs and embryos,as well as the preservation of skin,fingers,and other isolated tissues.However,cryopreservation of large and complex tissues or organs remains highly challenging.In addition to the damage caused by the freezing and rewarming processes and the inherent complexity of tissues and organs,there is an urgent need to address issues related to damage detection and the investigation of injury mechanisms.It provides a retrospective analysis of existing methods for assessing tissue and organ viability.Although current techniques can detect damage to some extent,they tend to be relatively simple,time-consuming,and limited in their ability to provide timely and comprehensive assessments of viability.By summarizing and evaluating these approaches,our study aims to contribute to the improvement of viability detection methods and to promote further development in this critical area.
文摘为研究梯度回温对低温贮藏荔枝货架期品质保持的作用,以“八月红”荔枝为实验材料,经过1 d 1℃冷库贮藏后,依次经过9、18和25℃三个温度环境后逐渐回到室温(25℃),设为“梯度回温”处理组,同时设置1 d 1℃冷库贮藏后直接放入25℃环境恢复室温(25℃)的荔枝为“直接回温”处理组作为对照,分别模拟货架在常温放置0、1、3、5 d,并对货架期荔枝果实各项指标进行测定。结果表明,“梯度回温”处理能够缓解荔枝褐变,回温处理后在常温放置5 d时,“直接回温”荔枝的褐变指数为1.50,“梯度回温”荔枝的褐变指数仅为1.00。此外检测发现“梯度回温”延缓了可溶性固形物、可滴定酸、总酚、类黄酮、花青素含量的减少,并有效抑制了果皮中多酚氧化酶和过氧化物酶活性的增加。因此,采用梯度回温方式处理能有效缓解低温贮藏荔枝品质劣变,延长货架保鲜期,具有广阔的应用前景。
