Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity le...Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity levels among different populations of tree peony varieties.The results showed that 34 bands were amplified using five pairs of cp SSR primers,with an average of 6.8 bands per primer pair.The average number of different alleles(N_(a)),effective alleles(N_(e)),Shannon's information index(I),diversity(H),and polymorphic information content(PIC)were 3.600,2.053,0.708,0.433,and 0.388,respectively.The PIC value was between 0.250 and 0.500,indicating a moderate level of polymorphism for the five cp SSR primer pairs.The genetic diversity levels of peony cultivars varied among different populations,with the Northwest population showing relatively lower levels(I=0.590,H=0.289,and PIC=0.263).A total of 52 haplotypes were identified in the four examined populations,and the number of haplotypes per population ranged from 11 to 22.Forty-four private haplotypes were detected across populations,and the Northwest population exhibiting the highest count of private haplotypes with 17.The mean number of effective number of haplotypes(N_(eh)),haplotypic richness(R_(h)),and diversity(H)were 8.351,6.824,and 0.893,respectively.Analysis of molecular variance indicated that genetic variation within tree peony germplasm was greater than that between germplasm resources,and the main variation was found within individuals of peony germplasm.Cluster analysis,principal coordinate analysis,and genetic structure analysis classified tree peonies from different origins into two groups,indicating a certain degree of genetic differentiation among these four tree peony cultivation groups.This study provides a theoretical basis for the exploration,utilization,and conservation of peony germplasm resources,as well as for research on the breeding of excellent varieties.展开更多
果肉颜色是猕猴桃重要的品质性状,不同果肉颜色的猕猴桃在色素成分、糖和维生素C含量等方面存在很大差异。为了探究猕猴桃果肉颜色形成的分子机制,本研究以红肉猕猴桃‘红实2号’和黄肉猕猴桃‘金实1号’为研究对象,采用RNA-seq技术,通...果肉颜色是猕猴桃重要的品质性状,不同果肉颜色的猕猴桃在色素成分、糖和维生素C含量等方面存在很大差异。为了探究猕猴桃果肉颜色形成的分子机制,本研究以红肉猕猴桃‘红实2号’和黄肉猕猴桃‘金实1号’为研究对象,采用RNA-seq技术,通过分析果实不同时期不同部位的转录组表达差异。结果表明,编码花青素合成的Achn385311(3GGT)基因可能是控制‘红实2号’内果皮呈红色的主效基因,‘金实1号’后期果肉呈深黄色可能与Achn158981(GT7)、Achn150731、Achn068721(PAO)、Achn282201(PAO)和Achn176251(UGT71A16)等基因的表达调控有关。针对差异表达的230个猕猴桃果肉颜色相关基因,利用Primer Premier 5.0设计出727对EST-SSR引物,以7份不同果肉颜色的猕猴桃基因组DNA为模板,随机选择112对引物进行有效性验证,筛选出了具有清晰的扩增产物的引物78对,有效扩增率为69.64%,58对具有多态性,多态性频率为74.36%。本研究结果为猕猴桃果实呈色机理研究及分子标记辅助育种提供了理论基础。展开更多
基金supported by Innovation Scientists and Technicians Troop Construction Projects of Henan Province(Grant No.212101510003)the Central Plains Scholar Workstation Project(Grant No.224400510002)+1 种基金the Youth Science Foundation of Henan Province(Grant No.202300410136)the Experimental Development Foundation of Henan University of Science and Technology(Grant No.SY2324004)。
文摘Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity levels among different populations of tree peony varieties.The results showed that 34 bands were amplified using five pairs of cp SSR primers,with an average of 6.8 bands per primer pair.The average number of different alleles(N_(a)),effective alleles(N_(e)),Shannon's information index(I),diversity(H),and polymorphic information content(PIC)were 3.600,2.053,0.708,0.433,and 0.388,respectively.The PIC value was between 0.250 and 0.500,indicating a moderate level of polymorphism for the five cp SSR primer pairs.The genetic diversity levels of peony cultivars varied among different populations,with the Northwest population showing relatively lower levels(I=0.590,H=0.289,and PIC=0.263).A total of 52 haplotypes were identified in the four examined populations,and the number of haplotypes per population ranged from 11 to 22.Forty-four private haplotypes were detected across populations,and the Northwest population exhibiting the highest count of private haplotypes with 17.The mean number of effective number of haplotypes(N_(eh)),haplotypic richness(R_(h)),and diversity(H)were 8.351,6.824,and 0.893,respectively.Analysis of molecular variance indicated that genetic variation within tree peony germplasm was greater than that between germplasm resources,and the main variation was found within individuals of peony germplasm.Cluster analysis,principal coordinate analysis,and genetic structure analysis classified tree peonies from different origins into two groups,indicating a certain degree of genetic differentiation among these four tree peony cultivation groups.This study provides a theoretical basis for the exploration,utilization,and conservation of peony germplasm resources,as well as for research on the breeding of excellent varieties.
文摘果肉颜色是猕猴桃重要的品质性状,不同果肉颜色的猕猴桃在色素成分、糖和维生素C含量等方面存在很大差异。为了探究猕猴桃果肉颜色形成的分子机制,本研究以红肉猕猴桃‘红实2号’和黄肉猕猴桃‘金实1号’为研究对象,采用RNA-seq技术,通过分析果实不同时期不同部位的转录组表达差异。结果表明,编码花青素合成的Achn385311(3GGT)基因可能是控制‘红实2号’内果皮呈红色的主效基因,‘金实1号’后期果肉呈深黄色可能与Achn158981(GT7)、Achn150731、Achn068721(PAO)、Achn282201(PAO)和Achn176251(UGT71A16)等基因的表达调控有关。针对差异表达的230个猕猴桃果肉颜色相关基因,利用Primer Premier 5.0设计出727对EST-SSR引物,以7份不同果肉颜色的猕猴桃基因组DNA为模板,随机选择112对引物进行有效性验证,筛选出了具有清晰的扩增产物的引物78对,有效扩增率为69.64%,58对具有多态性,多态性频率为74.36%。本研究结果为猕猴桃果实呈色机理研究及分子标记辅助育种提供了理论基础。
