摘要
【目的】皱皮木瓜(Chaenomeles speciosa)具有较高的观赏价值食用价值与药用价值,我国是皱皮木瓜的起源分布中心,研究皱皮木瓜种质资源遗传多样性,并构建品种分子身份证,以解决近年来因缺乏品种间统一的分类标准,同名异种、同种异名和品种间来源及演化不清等问题。【方法】以收集的168份皱皮木瓜种质资源为材料,利用SSR标记结合毛细管电泳法对木瓜遗传多样性和群体内遗传分化程度进行分析,根据观测等位基因数(N_(a))、Shannon's信息指数(I)和多态性信息含量(PIC)的筛选能区分全部种质的引物组合,并基于字符串编码构建DNA分子身份证。【结果】26对引物在168个皱皮木瓜品种中共扩增出304个等位基因,平均每个位点扩增出11.577个;期望杂合度(H_(e))、I和PIC的平均值分别为0.748、1.731和0.607。根据聚类分析的结果可将该群体分为2大类,进一步分为6个类群;种群结构分析将供试材料分为2个亚类。从26对引物中筛选出11对核心引物构建了168份皱皮木瓜种质资源的条形码和二维码身份证。【结论】所选扩增位点的变异程度高、鉴别力度大,在进行遗传多样性分析、核心引物筛选,以及指纹图谱构建中可优先选用。该研究为皱皮木瓜的良种鉴定、遗传资源管理及种质资源数据库的构建等提供了参考。
【Objective】Chaenomeles speciosa has high ornamental,edible,and medicinal values.China is the origin and distribution center of C.speciosa.The genetic diversity of Chaenomeles speciosa germplasm resources was studied,and the molecular identity card of varieties was constructed to solve the problems of lack of unified classification criteria among varieties,homonymous and synoonymous,and unclear origin and evolution among varieties in recent years.【Method】A total of 168 C.speciosa varieties and SSR(simple sequence repeats)markers were combined with capillary electrophoresis to analyze the genetic diversity and the degree of genetic differentiation.The observed number of alleles(N a),Shannon s information index(I),and the polymorphism information content(PIC)were employed to screen primer combinations that can distinguish the entire germplasm and construct DNA molecular IDs based on string codes.【Result】The results showed that 26 pairs of primers amplified 304 alleles in 168 C.speciosa varieties,with an average of 11.577 per locus.The average expected heterozygosity(H e),I and PIC were determined as 0.748,1.731 and 0.607,respectively.Based on the cluster analysis,the population could be divided into two groups,and further into six groups.Moreover,based on population structure analysis,the tested materials was divided into two subgroups.Four pairs of core primers were selected from 26 pairs of primers to construct barcode and two-dimensional code identification cards of C.speciosa varieties.【Conclusion】The selected amplification loci have a high degree of variation and strong discrimination,and are thus suitable for applications in genetic diversity analysis,core primer screening,and fingerprint construction.The results can provide a reference for the variety identification,genetic resource management,and the construction of a germplasm resource database of C.speciosa.
作者
李慧
侯立娜
王天琪
毕宁宁
李圣波
刘忠华
LI Hui;HOU Lina;WANG Tianqi;BI Ningning;LI Shengbo;LIU Zhonghua(National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental,Plants of Ministry of Education,Key Open Laboratory of Flower Breeding and Bioengineering,National Forestry and Grassland Administration,Beijing Forestry University,Beijing 100083,China;College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China;Shandong Yate Eco-tech,Co.,Ltd.,Junan 266071,China)
出处
《南京林业大学学报(自然科学版)》
北大核心
2025年第1期59-68,I0001,I0002,共12页
Journal of Nanjing Forestry University:Natural Sciences Edition
基金
国家林业和草原局业务委托项目(DNA-2021)
北京市园林绿化局计划(2021-STBHXFC-04-11)
陕西省榆林市榆阳区林业局服务项目(2024HXFW059)。
