[Objective]The aim was to study homology between Ran gene in Allium cepa,Allium sativum and Brassica napus and Ran2 gene in Arabidopsis in order to determine whether three kinds of plant material as substitute for Ara...[Objective]The aim was to study homology between Ran gene in Allium cepa,Allium sativum and Brassica napus and Ran2 gene in Arabidopsis in order to determine whether three kinds of plant material as substitute for Arabidopsis. [Method]By using RT-PCR method,homology gene was cloned from totoal RNA which extracted from splinter cells of Allium cepa,Allium sativum and Brassica napus with Arabidopsis Ran2 primer,then,carrying out sequence and comparative analysis. [Result]The results showed that the open reading frames of Ran genes in Allium cepa,Allium sativum and Brassica napus were 666,663,666 bp,coding 221,220 and 221 amino acids respectively,with the molecular weight of 24.3 kDa. The sequence analysis showed that the amino acid homology of Ran genes between Allium cepa,Allium sativum,Brassica napus and Arabidopsis Ran2 were respectively 99.1 %,100 % （except an Asp D at Allium sativum C terminal）,96.4 %. The phylogenetic tree indicated that Ran genes from Allium cepa and Allium sativum had closer evolutionary relationship with Arabidopsis Ran2. [Conclusion]The research laid a foundation for further study on the biological function of plant Ran gene.展开更多
Ran is an evolutionarily conserved eukaryotic GTPase that directly participates in cell cycle and whose loss affects many biological processes.We have identified cDNA of TaRAN/,a novel Ran GTPase homologous gene in wh...Ran is an evolutionarily conserved eukaryotic GTPase that directly participates in cell cycle and whose loss affects many biological processes.We have identified cDNA of TaRAN/,a novel Ran GTPase homologous gene in wheat(Triticum aestivum L.cv.Jingdong No.1).The cytoplasmic microtu-bules play an important role in cytoplasmic organization,cell division,and the correct transmission of genetic information in fission yeast cell.Using the fission yeast system in vivo experiments,overexpression of TaRAN/produced defective spindle microtubules,probably resulting in chromosome missegregation we reported previously.The microtubules of antisense TaRANI yeast cells were physically disrupted.This suggested that TaRAN/plays a role in mitotic spindle assembly and microtubule integrity and stability.Ultrastructural analysis under transmission electron microscope(TEM)showed abnormal nuclear membranes in the overexpression TaRAN/yeast cells,abnormal vacuole structures and disorganized membranes in the antisense TaRANI yeast cells.These results suggested that TaRANI was essential for all nucleocytoplasmic transport events.展开更多
基金Supported by the National Research Program for High Technology Ship Development(No.MIIT 2014-498)the Science and Technology Project of Guangdong Province(2016B090918092)the Marine Renewable Energy Special Fund(QDME2013ZB01)
基金Supported by National Natural Science Foundation of China(30070370)~~
文摘[Objective]The aim was to study homology between Ran gene in Allium cepa,Allium sativum and Brassica napus and Ran2 gene in Arabidopsis in order to determine whether three kinds of plant material as substitute for Arabidopsis. [Method]By using RT-PCR method,homology gene was cloned from totoal RNA which extracted from splinter cells of Allium cepa,Allium sativum and Brassica napus with Arabidopsis Ran2 primer,then,carrying out sequence and comparative analysis. [Result]The results showed that the open reading frames of Ran genes in Allium cepa,Allium sativum and Brassica napus were 666,663,666 bp,coding 221,220 and 221 amino acids respectively,with the molecular weight of 24.3 kDa. The sequence analysis showed that the amino acid homology of Ran genes between Allium cepa,Allium sativum,Brassica napus and Arabidopsis Ran2 were respectively 99.1 %,100 % （except an Asp D at Allium sativum C terminal）,96.4 %. The phylogenetic tree indicated that Ran genes from Allium cepa and Allium sativum had closer evolutionary relationship with Arabidopsis Ran2. [Conclusion]The research laid a foundation for further study on the biological function of plant Ran gene.
文摘Ran is an evolutionarily conserved eukaryotic GTPase that directly participates in cell cycle and whose loss affects many biological processes.We have identified cDNA of TaRAN/,a novel Ran GTPase homologous gene in wheat(Triticum aestivum L.cv.Jingdong No.1).The cytoplasmic microtu-bules play an important role in cytoplasmic organization,cell division,and the correct transmission of genetic information in fission yeast cell.Using the fission yeast system in vivo experiments,overexpression of TaRAN/produced defective spindle microtubules,probably resulting in chromosome missegregation we reported previously.The microtubules of antisense TaRANI yeast cells were physically disrupted.This suggested that TaRAN/plays a role in mitotic spindle assembly and microtubule integrity and stability.Ultrastructural analysis under transmission electron microscope(TEM)showed abnormal nuclear membranes in the overexpression TaRAN/yeast cells,abnormal vacuole structures and disorganized membranes in the antisense TaRANI yeast cells.These results suggested that TaRANI was essential for all nucleocytoplasmic transport events.
