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拟南芥Ran小GTP结合蛋白在细胞有丝分裂中的定位 被引量：3

The Localization of Arabidopsis thaliana Ran Small GTP-binding Protein in Cell Mitosis
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摘要 分别采用RT-PCR和直接荧光免疫方法分析Ran2 mRNA在拟南芥不同组织器官中的表达及Ran2的亚细胞定位。结果表明,Ran2基因在拟南芥根、茎、叶和花中均有表达;Ran2蛋白在细胞分裂间期主要定位于核膜周边,在后期定位于赤道板上和纺锤体上,末期又回到子细胞核膜周边。 The expressionof .Arabidopsis Ran2 mRNA were analysed in different tissues by RTPCR, and the direct immunofluorescence microscopy was used to determine the subcellular localization of plant Ran proteins during the cell cycle. The results showed that Ran2 is expressed ubiquitously in Arabidopsis roots, stems, leaves and flowers. Ran2 protein was mainly localized at the nuclear envelope during interphase,at the equatorial plate and spindle during anaphase and at the surface of the daughter nuclei during telophase.
作者 马立安 江涛 张忠明
机构地区 长江大学生命科学学院 华中农业大学农业微生物学国家重点实验室 长江大学动物科学学院
出处 《华中农业大学学报》 CAS CSCD 北大核心 2008年第6期701-704,共4页 Journal of Huazhong Agricultural University
基金 国家自然科学基金项目(30070370) 美国国家科学基金项目(NSF-MCB0548525)资助
关键词 拟南芥Ran2小GTP结合蛋白 FITC-anti-Ran2抗体 亚细胞定位 Arabidopsis Ran2 small GTP-binding protein anti-Ran2 labelled with FITC subcellular localization
分类号 Q7 [生物学—分子生物学]
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参考文献9

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  • 5马立安,江涛,张忠明.拟南芥Ran2小GTP结合蛋白抗血清的制备及其FITC荧光标记[J].华中农业大学学报,2007,26(4):439-442. 被引量：2
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二级参考文献11

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  • 2江涛,周艳琴,刘琴,聂浩,姚宝安,赵俊龙.弓形虫微线体蛋白MIC3基因的克隆及原核表达[J].畜牧兽医学报,2006,37(6):587-591. 被引量：16
  • 3马立安,江涛,张忠明.拟南芥Ran2基因的原核表达及产物的纯化[J].长江大学学报（自科版）（中旬）,2007,4(1):45-47. 被引量：6
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共引文献6

同被引文献20

  • 1王昕,种康.植物小G蛋白功能的研究进展[J].植物学通报,2005,22(1):1-10. 被引量：20
  • 2马立安,江涛,张忠明.拟南芥Ran2基因的原核表达及产物的纯化[J].长江大学学报（自科版）（中旬）,2007,4(1):45-47. 被引量：6
  • 3JIANG S Y,RAMACHANDRAN S. Comparative and evolutionary analysis of genes encoding small GTPases and their activating proteins in eukaryotic genomes [J]. Physiol Genomics ,2006 ,24 :2.35 -251.
  • 4CLARKE P R,ZHANG C M. Ran GTPase:a master regulator of nuclear structure and function during the eukaryotic cell division cycle [ J ]. J Trends Cell Biol,2001,11:366 -371.
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  • 6MA L,HONG Z,ZHANG Z. Perinuclear and nuclear envelope localizations of Arabidopsis Ran proteins [ J ]. Plant Cell Reports (Plant Cell Rep ), 21307,26(8) :1373 - 1382.
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  • 8JIANG SY, RAMACHANDRAN S. Comparative and evolutionary analysis of genes encoding small GTPases and their activating proteins in eukaryotic genomes [J]. Physiol Genomics, 2006, 24: 235 -251.
  • 9CLARKE PR, ZHANG CM. Ran GTPase, a master regulator of nuclear structure and function during the eukaryotic cell division cycte? [J]. J Trends Cell Biol, 2001, 11: 366 -371.
  • 10MOORE JD. The Ran-GTPase and cell-cycle control [J]. J Bioessays, 2001,23:77-85.

引证文献3

二级引证文献2

华中农业大学学报
2008年 第6期

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