This study was carried out to identify gene expression patterns in different wool densities of rex rabbit. The mid-dorsal skin samples from 8 rex rabbits were studied. They were divided into two groups according to di...This study was carried out to identify gene expression patterns in different wool densities of rex rabbit. The mid-dorsal skin samples from 8 rex rabbits were studied. They were divided into two groups according to different wool densities. Total RNA was isolated and labeled by reverse transcription reaction with Cy5-dCTP and Cy3-dCTP for cDNA probe. The cDNA probe was hybridized with cDNA microarrays containing 14 601 rabbit’s genes. The differently expressed genes were analysed with the Gene Ontology （GO） classification and the pathway analysis. Hierarchical clustering was performed to clarify genes in association with different wool densities. The 2 657 differentially expressed genes were identified. Among them, 1 103 genes were functionally known genes, 687 genes were up-regulated and 419 were down-regulated. GO analysis indicated that these altered genes were associated with metabolism, signal transduction, cell cycle, cell adhesion, cell proliferation, cell division, apoptosis, and other processes. KEGG analysis showed that 95 signal pathways associated with up-regulated genes and 87 signal pathways associated with down-regulated genes had changed significantly （P0.05）. Some important differentially expressed genes in different wool densities of rex rabbit were identified, such as MMP2, TGF-β1, TGF-β2, IGF-1, ITGB1, RPS6KB1, BMP2, ActRIIB, CDK2, and CCNA2. Hierarchical clustering analysis separated the differentially expressed genes into 5 main characteristic groups. Data from cDNA microarray experiments clearly distinguished between group A and group B. Some important genes were identified, which might be useful in further study on wool density markers of rex rabbit.展开更多
本研究旨在筛选4日龄不同被毛密度獭兔皮肤的差异表达基因。采用Agilent公司家兔全基因表达谱芯片对4日龄不同被毛密度獭兔皮肤组织进行差异基因筛选,并通过实时荧光定量PCR方法对部分差异显著基因进行验证。试验共筛选出188个差异基因...本研究旨在筛选4日龄不同被毛密度獭兔皮肤的差异表达基因。采用Agilent公司家兔全基因表达谱芯片对4日龄不同被毛密度獭兔皮肤组织进行差异基因筛选,并通过实时荧光定量PCR方法对部分差异显著基因进行验证。试验共筛选出188个差异基因,与低被毛密度獭兔相比,高被毛密度獭兔皮中表达上调基因72个,其中包含24个已知功能基因,表达下调基因116个,其中包含38个已知功能基因。GO(Gene ontology)分类显示,大部分差异基因参与细胞增殖、凋亡、氧化还原、脂代谢分解等生命过程。KEGG(Kyoto encyclopedia of genes and genomes)通路分析显示,差异基因主要参与Wnt信号通路、PPAR信号通路、维生素合成与代谢途径等。荧光定量PCR结果与基因芯片结果基本吻合,证实基因芯片结果可靠。结果表明,差异倍数较大的差异基因,像胰岛素样生长因子(IGF-I)、血管内皮生长因子(VEGF)和骨形态发生蛋白(BMP2)可能与獭兔被毛的形成有关;Wnt信号通路、PPAR信号通路等,以及维生素合成与代谢途径等也可能参与被毛的发育过程。展开更多
基金supported by the Science and Technol- ogy Support Project of Hebei, China (06220402D-4)the Modern Agriculture (Rabbit) Industrial Science and Technology System, China (nycyti-44)
文摘This study was carried out to identify gene expression patterns in different wool densities of rex rabbit. The mid-dorsal skin samples from 8 rex rabbits were studied. They were divided into two groups according to different wool densities. Total RNA was isolated and labeled by reverse transcription reaction with Cy5-dCTP and Cy3-dCTP for cDNA probe. The cDNA probe was hybridized with cDNA microarrays containing 14 601 rabbit’s genes. The differently expressed genes were analysed with the Gene Ontology （GO） classification and the pathway analysis. Hierarchical clustering was performed to clarify genes in association with different wool densities. The 2 657 differentially expressed genes were identified. Among them, 1 103 genes were functionally known genes, 687 genes were up-regulated and 419 were down-regulated. GO analysis indicated that these altered genes were associated with metabolism, signal transduction, cell cycle, cell adhesion, cell proliferation, cell division, apoptosis, and other processes. KEGG analysis showed that 95 signal pathways associated with up-regulated genes and 87 signal pathways associated with down-regulated genes had changed significantly （P0.05）. Some important differentially expressed genes in different wool densities of rex rabbit were identified, such as MMP2, TGF-β1, TGF-β2, IGF-1, ITGB1, RPS6KB1, BMP2, ActRIIB, CDK2, and CCNA2. Hierarchical clustering analysis separated the differentially expressed genes into 5 main characteristic groups. Data from cDNA microarray experiments clearly distinguished between group A and group B. Some important genes were identified, which might be useful in further study on wool density markers of rex rabbit.
文摘本研究旨在筛选4日龄不同被毛密度獭兔皮肤的差异表达基因。采用Agilent公司家兔全基因表达谱芯片对4日龄不同被毛密度獭兔皮肤组织进行差异基因筛选,并通过实时荧光定量PCR方法对部分差异显著基因进行验证。试验共筛选出188个差异基因,与低被毛密度獭兔相比,高被毛密度獭兔皮中表达上调基因72个,其中包含24个已知功能基因,表达下调基因116个,其中包含38个已知功能基因。GO(Gene ontology)分类显示,大部分差异基因参与细胞增殖、凋亡、氧化还原、脂代谢分解等生命过程。KEGG(Kyoto encyclopedia of genes and genomes)通路分析显示,差异基因主要参与Wnt信号通路、PPAR信号通路、维生素合成与代谢途径等。荧光定量PCR结果与基因芯片结果基本吻合,证实基因芯片结果可靠。结果表明,差异倍数较大的差异基因,像胰岛素样生长因子(IGF-I)、血管内皮生长因子(VEGF)和骨形态发生蛋白(BMP2)可能与獭兔被毛的形成有关;Wnt信号通路、PPAR信号通路等,以及维生素合成与代谢途径等也可能参与被毛的发育过程。
