The Xingnao Jieyu capsule has been shown to effectively relieve neurologic impairments and les- sen depression. It remains poorly understood whether this capsule can be used to treat post-stroke depression. Thus, in t...The Xingnao Jieyu capsule has been shown to effectively relieve neurologic impairments and les- sen depression. It remains poorly understood whether this capsule can be used to treat post-stroke depression. Thus, in the present study, we established a rat model of post-stroke depression using left middle cerebral artery occlusions in combination of chronic unpredictable stress and solitary housing during development. Experimental rats received intragastric perfusion with 0.82, 0.41, and 0.20 g/kg Xingnao Jieyu capsules separately dissolved in 2 mL distilled water. Fluoxetine served as a positive control. The treatment was conducted over 28 days. Sugar water consumption test, open-field test, real-time fluorescent quantitative PCR and immunohistochemical staining results demonstrated that intragastric perfusion with various doses of Xingnao Jieyu capsules increased sugar water consumption, voluntary behaviors and synaptotagmin mRNA and protein expression in rats with post-stroke depression. These therapeutic effects were similar to those of fluoxetine. These results indicate that Xingnao Jieyu capsules upregulate synaptotagmin expression in hip pocampi of rats with post-stroke depression, and exert antidepressant effects.展开更多
Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombin...Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombinant empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production. A plasmid containing the capsid precursor P12A and protease 3C sequences of foot-and-mouth disease virus (FMDV) was constructed and used to compare transient and stable expression in mammalian cells. When BHK-21 cells were transfected with the recombinant vector, protease 3C cleaved the capsid precursor P12A into the structural proteins VP0, VP1 and VP3. A sucrose gradient demonstrated that the structural proteins assembled into different subviral particles. Attempts to generate a stable cell line only allowed isolating low-level-expressing clones, probably due to the effect of protease 3C on the cells. Moreover, the recombinant protein yield achieved in transient expression assays was much higher than the one achieved in stable expression assays. Results indicate that mammalian cells are a good strategy to produce recombinant FMDV subviral particles. However, the alternative approach of transient gene expression in scalable systems should be used instead of the standard method that involves the generation of a stable cell line.展开更多
A new method to extract person-independent expression feature based on higher-order singular value decomposition （HOSVD） is proposed for facial expression recognition. Based on the assumption that similar persons ha...A new method to extract person-independent expression feature based on higher-order singular value decomposition （HOSVD） is proposed for facial expression recognition. Based on the assumption that similar persons have similar facial expression appearance and shape, the person-similarity weighted expression feature is proposed to estimate the expression feature of test persons. As a result, the estimated expression feature can reduce the influence of individuals caused by insufficient training data, and hence become less person-dependent. The proposed method is tested on Cohn-Kanade facial expression database and Japanese female facial expression （JAFFE） database. Person-independent experimental results show the superiority of the proposed method over the existing methods.展开更多
A Discrete Element Method （DEM） model is developed to study the particle break- age effect on the one-dimensional compression behavior of silica sands. The ＇maximum tensile stress＇ breakage criterion considering m...A Discrete Element Method （DEM） model is developed to study the particle break- age effect on the one-dimensional compression behavior of silica sands. The ＇maximum tensile stress＇ breakage criterion considering multiple contacts is adopted to simulate the crushing of circular particles in the DEM. The model is compared with published experimental results. Com- parison between the compression curves obtained from the numerical and experimental results shows that the proposed method is very effective in studying the compression behavior of silica sands considering particle breakage. The evolution of compression curves at different stress levels is extensively studied using contact force distribution, variation of contact number and particle size distribution curve with loading. It is found that particle breakage has great impact on com- pression behavior of sand, particularly after the yield stress is reached and particle breakage starts. The crushing probability of particles is found to be macroscopically affected by stress level and particle size distribution curve, and microscopically related to the evolutions of contact force and coordination number. Once the soil becomes well-graded and the average coordination number is greater than 4 in two-dimension, the crushing probability of parent particles can reduce by up to 5/6. It is found that the average contact force does not always increase with loading, but increases to a peak value then decreases once the soil becomes more well-graded. It is found through the loading rate sensitivity analysis that the compression behavior of sand samples in the DEM is also affected by the loading rate. Higher yield stresses are obtained at higher loading rates.展开更多
A grating lobes suppression method for chirp-subpulse stepped frequency（CSSF） signals is proposed, which is applied to deformation monitoring using the ground based synthetic aperture radar（GB-SAR） system. This me...A grating lobes suppression method for chirp-subpulse stepped frequency（CSSF） signals is proposed, which is applied to deformation monitoring using the ground based synthetic aperture radar（GB-SAR） system. This method is based on accurate estimation and correction of the phase and amplitude error along two dimensions（range and azimuth）, i.e., the error estimation inside the subpulse（in-subpulse error） and across the stepped frequency subpulses（cross-subpulse error） of transmitted CSSF signals. Validated both with simulated data and experimental data recorded in the deformation monitoring campaign, it can be seen that the method as well as the relative conclusions can be fully and effectively applied to most of the stepped frequency systems.展开更多
[Objective] The differential expression analysis was performed for FaGF14- B and FaGF14-C genes in tall fescue so as to provide certain basis for follow-up functional analysis of genes. [Method] The sequence fragments...[Objective] The differential expression analysis was performed for FaGF14- B and FaGF14-C genes in tall fescue so as to provide certain basis for follow-up functional analysis of genes. [Method] The sequence fragments of FaGF14-B and FaGF14-C obtained from reverse transcription were used as templates, and the full- length cDNA sequences of FaGF14-B and FaGF14-C were amplified using the 5＇ RACE use 3＇RACE techniques. They were named as FaGF14-B and FaGF14-C, and used for nucleic acid sequence analysis, encoded protein analysis, protein con- served domain analysis, phylogenetic analysis and differential expression analysis. [Result] The FaGF14-B gene has a full length of 1 548 bp. It has a complete open reading frame （ORF, 449-1 228 bp）, and encodes a protein composed of 261 amino acids. The FaGF14-C gene has a full length of 1 250 bp. It also has a complete open reading frame （ORF, 66-848 bp）, and encodes a protein composed of 261 amino acids. The GF14-B and GF14-C proteins all have a typical domain 14-3-3, and their secondary structures all contain 9 conserved co-helical structures and non-conserved N- and C- terminals. The phylogenetic analysis showed that the FaGF14-B and FaGF14-C from tall rescue have high similarities with GF14 protein from gramineous plants, and they are divided into the same clade with closer ge- netic relationship. The real-time fluorescence quantitative PCR analysis showed that the expression of FaGF14-B and FaGF14-C genes is all sensitive to nitrogen stress. [Conclusion] This study will lay a theoretical basis for further screening of low nitrogen-tolerant genes and breeding of low nitrogen-tolerant grass germplasms.展开更多
Totally 20 cases of mental depression were treated by needling extrachannel points with an effec-tive rate of 90%; comparison of these cases with 21 controls treated with amitriptyline, showed no significant differenc...Totally 20 cases of mental depression were treated by needling extrachannel points with an effec-tive rate of 90%; comparison of these cases with 21 controls treated with amitriptyline, showed no significant difference in effect (P】0.05). Measured with Hamilton’s scale, factors of anxiety somatization, cognitive disturbance, retardation, sleep disturbance and feeling of despair in both the needling and control groups showed obvious decrease in the mean value, and the change in anxiety somatization was markedly significant in the needling group as compared with that in the controls (P【 0.01). Our studies revealed that after 6 weeks of needling the power of slow wave 5 decreased while that of the fast wave a increased, all significantly different as compared with that before and during the treatment (P 【 0.05).展开更多
Objective To investigate the prevalence of BRAF T1799A mutation and RET/PTC rearrangement in Qingdao and detect the expression of platelet-derived growth factor B(PDGF-B) in order to investigate the
Let P be a cone in a real Banach space E. Let sr={x|||x||=r}, SR={x|||x||=R}, Tr,R={x|r<||x||<R} and Tr,R={x|r≤||x||≤R}（R>r>0）. The fixed point theorem about the expansion and compression of a cone can...Let P be a cone in a real Banach space E. Let sr={x|||x||=r}, SR={x|||x||=R}, Tr,R={x|r<||x||<R} and Tr,R={x|r≤||x||≤R}（R>r>0）. The fixed point theorem about the expansion and compression of a cone can be formulated as follows.展开更多
Objective To explore the role of receptor-interacting protein kinase 3(RIP3)-mediated necroptosis in ulcerative colitis(UC).Methods The colonic mucosa tissues of eighty-five patients diagnosed with UC were collected.D...Objective To explore the role of receptor-interacting protein kinase 3(RIP3)-mediated necroptosis in ulcerative colitis(UC).Methods The colonic mucosa tissues of eighty-five patients diagnosed with UC were collected.Disease staging and pathological classification of UC patients were evaluated according to Mayo standard展开更多
Objective To explore the expression of thyroid stimulating hormone (TSH) receptor in differentiated thyroid carcinoma and its clinical significance.Methods Seventy-four patients with differentiated thyroid carcinoma t...Objective To explore the expression of thyroid stimulating hormone (TSH) receptor in differentiated thyroid carcinoma and its clinical significance.Methods Seventy-four patients with differentiated thyroid carcinoma treated in our department from January 2009 to January 2011were selected as the observation group,and 28 patients with nodular goiter were selected as the control group.Expression of TSH receptor in the two groups were detected by immunohistochemistry.Results The positive rate of TSH receptor expression in the observation group was55.4 (41/74) ,significantly lower than that of the展开更多
Objective To explore the expression and clinical significance of Semaphorin4D (Sema4D) mRNA in peripheral blood lymphocyte,Sema4D on platelet surface, soluble Sema4D (sSema4D) in plasma in patients with cerebral infar...Objective To explore the expression and clinical significance of Semaphorin4D (Sema4D) mRNA in peripheral blood lymphocyte,Sema4D on platelet surface, soluble Sema4D (sSema4D) in plasma in patients with cerebral infarction. Methods Taking 299 patients with cerebral展开更多
Objective to explore the clinical value of miRNA-29b expression and the combined detection of serum miRNA-29b and alpha-fetoprotein(AFP)in the diagnosis of primary hepatic carcinoma(PHC).Methods
Objective To evaluate estrogen receptor(ER)α and β expressions in elderly patients with non-small cell lung cancer(NSCLC) in China and their relationship with clinical and pathological
Objective To explore the expression and pathological features of insulin-like growth factor-Ⅱ(IGF-Ⅱ)in tissues and sera of hepatocellular carcinoma(HCC) patients and the siRNA-mediated inhibition
Objective To examine the expression of follistatinlike protein 1(FSTL1)in the peripheral blood and intestinal mucosa tissues of ulcerative colitis(UC)patients and analyze the correlation between its expression and the...Objective To examine the expression of follistatinlike protein 1(FSTL1)in the peripheral blood and intestinal mucosa tissues of ulcerative colitis(UC)patients and analyze the correlation between its expression and the activity of UC.Methods From October 2010 to J une2012,sixty patients with UC were collected.From展开更多
Objective: To observe the effects of leptin on glucose oxidation and glueokinase gene expression in rat liver cells. Methods: Rat liver cells were incubated with leptin of different doses (ranging from 10μg/L to 200...Objective: To observe the effects of leptin on glucose oxidation and glueokinase gene expression in rat liver cells. Methods: Rat liver cells were incubated with leptin of different doses (ranging from 10μg/L to 200μg/L). Control liver cells展开更多
Objective To investigate the expression of survivin in leukemia and the prognostic significance in acute leukemia(AL). Methods The expression of survivin mRNA was measured in 105 AL and 21 chronic myelogenous leukemia...Objective To investigate the expression of survivin in leukemia and the prognostic significance in acute leukemia(AL). Methods The expression of survivin mRNA was measured in 105 AL and 21 chronic myelogenous leukemia (CML) patients with semi-quantity reverse transcription (RT)-PCR.15 adults were tested as展开更多
基金funded by the Key Science and Technology Project of Shaanxi Provincial "13115"Technology Innovation Engineering,No.2010ZDKG-65
文摘The Xingnao Jieyu capsule has been shown to effectively relieve neurologic impairments and les- sen depression. It remains poorly understood whether this capsule can be used to treat post-stroke depression. Thus, in the present study, we established a rat model of post-stroke depression using left middle cerebral artery occlusions in combination of chronic unpredictable stress and solitary housing during development. Experimental rats received intragastric perfusion with 0.82, 0.41, and 0.20 g/kg Xingnao Jieyu capsules separately dissolved in 2 mL distilled water. Fluoxetine served as a positive control. The treatment was conducted over 28 days. Sugar water consumption test, open-field test, real-time fluorescent quantitative PCR and immunohistochemical staining results demonstrated that intragastric perfusion with various doses of Xingnao Jieyu capsules increased sugar water consumption, voluntary behaviors and synaptotagmin mRNA and protein expression in rats with post-stroke depression. These therapeutic effects were similar to those of fluoxetine. These results indicate that Xingnao Jieyu capsules upregulate synaptotagmin expression in hip pocampi of rats with post-stroke depression, and exert antidepressant effects.
文摘Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombinant empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production. A plasmid containing the capsid precursor P12A and protease 3C sequences of foot-and-mouth disease virus (FMDV) was constructed and used to compare transient and stable expression in mammalian cells. When BHK-21 cells were transfected with the recombinant vector, protease 3C cleaved the capsid precursor P12A into the structural proteins VP0, VP1 and VP3. A sucrose gradient demonstrated that the structural proteins assembled into different subviral particles. Attempts to generate a stable cell line only allowed isolating low-level-expressing clones, probably due to the effect of protease 3C on the cells. Moreover, the recombinant protein yield achieved in transient expression assays was much higher than the one achieved in stable expression assays. Results indicate that mammalian cells are a good strategy to produce recombinant FMDV subviral particles. However, the alternative approach of transient gene expression in scalable systems should be used instead of the standard method that involves the generation of a stable cell line.
基金supported by National Natural Science Foundation of China (6087208460940008)+2 种基金Beijing Training Programming Foundation for the Talents (20081D1600300343)Excellent Young Scholar Research Fund of Beijing Institute of Technology (2007Y0305)Fundamental Research Foundation of Beijing Institute of Technology (20080342005)
文摘A new method to extract person-independent expression feature based on higher-order singular value decomposition （HOSVD） is proposed for facial expression recognition. Based on the assumption that similar persons have similar facial expression appearance and shape, the person-similarity weighted expression feature is proposed to estimate the expression feature of test persons. As a result, the estimated expression feature can reduce the influence of individuals caused by insufficient training data, and hence become less person-dependent. The proposed method is tested on Cohn-Kanade facial expression database and Japanese female facial expression （JAFFE） database. Person-independent experimental results show the superiority of the proposed method over the existing methods.
基金Project supported by the National Natural Science Foundation of China(Nos.50909057,51208294 and 41372319)the Innovation Program of Shanghai Municipal Education Commission(No.15ZZ081)the Innovation Project of Shanghai Postgraduate Education(No.20131129)
文摘A Discrete Element Method （DEM） model is developed to study the particle break- age effect on the one-dimensional compression behavior of silica sands. The ＇maximum tensile stress＇ breakage criterion considering multiple contacts is adopted to simulate the crushing of circular particles in the DEM. The model is compared with published experimental results. Com- parison between the compression curves obtained from the numerical and experimental results shows that the proposed method is very effective in studying the compression behavior of silica sands considering particle breakage. The evolution of compression curves at different stress levels is extensively studied using contact force distribution, variation of contact number and particle size distribution curve with loading. It is found that particle breakage has great impact on com- pression behavior of sand, particularly after the yield stress is reached and particle breakage starts. The crushing probability of particles is found to be macroscopically affected by stress level and particle size distribution curve, and microscopically related to the evolutions of contact force and coordination number. Once the soil becomes well-graded and the average coordination number is greater than 4 in two-dimension, the crushing probability of parent particles can reduce by up to 5/6. It is found that the average contact force does not always increase with loading, but increases to a peak value then decreases once the soil becomes more well-graded. It is found through the loading rate sensitivity analysis that the compression behavior of sand samples in the DEM is also affected by the loading rate. Higher yield stresses are obtained at higher loading rates.
基金supported by the National Natural Science Foundation of China(6112010600461225005)
文摘A grating lobes suppression method for chirp-subpulse stepped frequency（CSSF） signals is proposed, which is applied to deformation monitoring using the ground based synthetic aperture radar（GB-SAR） system. This method is based on accurate estimation and correction of the phase and amplitude error along two dimensions（range and azimuth）, i.e., the error estimation inside the subpulse（in-subpulse error） and across the stepped frequency subpulses（cross-subpulse error） of transmitted CSSF signals. Validated both with simulated data and experimental data recorded in the deformation monitoring campaign, it can be seen that the method as well as the relative conclusions can be fully and effectively applied to most of the stepped frequency systems.
基金Supported by National Natural Science Foundation of China(31360576)~~
文摘[Objective] The differential expression analysis was performed for FaGF14- B and FaGF14-C genes in tall fescue so as to provide certain basis for follow-up functional analysis of genes. [Method] The sequence fragments of FaGF14-B and FaGF14-C obtained from reverse transcription were used as templates, and the full- length cDNA sequences of FaGF14-B and FaGF14-C were amplified using the 5＇ RACE use 3＇RACE techniques. They were named as FaGF14-B and FaGF14-C, and used for nucleic acid sequence analysis, encoded protein analysis, protein con- served domain analysis, phylogenetic analysis and differential expression analysis. [Result] The FaGF14-B gene has a full length of 1 548 bp. It has a complete open reading frame （ORF, 449-1 228 bp）, and encodes a protein composed of 261 amino acids. The FaGF14-C gene has a full length of 1 250 bp. It also has a complete open reading frame （ORF, 66-848 bp）, and encodes a protein composed of 261 amino acids. The GF14-B and GF14-C proteins all have a typical domain 14-3-3, and their secondary structures all contain 9 conserved co-helical structures and non-conserved N- and C- terminals. The phylogenetic analysis showed that the FaGF14-B and FaGF14-C from tall rescue have high similarities with GF14 protein from gramineous plants, and they are divided into the same clade with closer ge- netic relationship. The real-time fluorescence quantitative PCR analysis showed that the expression of FaGF14-B and FaGF14-C genes is all sensitive to nitrogen stress. [Conclusion] This study will lay a theoretical basis for further screening of low nitrogen-tolerant genes and breeding of low nitrogen-tolerant grass germplasms.
文摘Totally 20 cases of mental depression were treated by needling extrachannel points with an effec-tive rate of 90%; comparison of these cases with 21 controls treated with amitriptyline, showed no significant difference in effect (P】0.05). Measured with Hamilton’s scale, factors of anxiety somatization, cognitive disturbance, retardation, sleep disturbance and feeling of despair in both the needling and control groups showed obvious decrease in the mean value, and the change in anxiety somatization was markedly significant in the needling group as compared with that in the controls (P【 0.01). Our studies revealed that after 6 weeks of needling the power of slow wave 5 decreased while that of the fast wave a increased, all significantly different as compared with that before and during the treatment (P 【 0.05).
文摘Objective To investigate the prevalence of BRAF T1799A mutation and RET/PTC rearrangement in Qingdao and detect the expression of platelet-derived growth factor B(PDGF-B) in order to investigate the
文摘Let P be a cone in a real Banach space E. Let sr={x|||x||=r}, SR={x|||x||=R}, Tr,R={x|r<||x||<R} and Tr,R={x|r≤||x||≤R}（R>r>0）. The fixed point theorem about the expansion and compression of a cone can be formulated as follows.
文摘Objective To explore the role of receptor-interacting protein kinase 3(RIP3)-mediated necroptosis in ulcerative colitis(UC).Methods The colonic mucosa tissues of eighty-five patients diagnosed with UC were collected.Disease staging and pathological classification of UC patients were evaluated according to Mayo standard
文摘Objective To explore the expression of thyroid stimulating hormone (TSH) receptor in differentiated thyroid carcinoma and its clinical significance.Methods Seventy-four patients with differentiated thyroid carcinoma treated in our department from January 2009 to January 2011were selected as the observation group,and 28 patients with nodular goiter were selected as the control group.Expression of TSH receptor in the two groups were detected by immunohistochemistry.Results The positive rate of TSH receptor expression in the observation group was55.4 (41/74) ,significantly lower than that of the
文摘Objective To explore the expression and clinical significance of Semaphorin4D (Sema4D) mRNA in peripheral blood lymphocyte,Sema4D on platelet surface, soluble Sema4D (sSema4D) in plasma in patients with cerebral infarction. Methods Taking 299 patients with cerebral
文摘Objective to explore the clinical value of miRNA-29b expression and the combined detection of serum miRNA-29b and alpha-fetoprotein(AFP)in the diagnosis of primary hepatic carcinoma(PHC).Methods
文摘Objective To evaluate estrogen receptor(ER)α and β expressions in elderly patients with non-small cell lung cancer(NSCLC) in China and their relationship with clinical and pathological
文摘Objective To explore the expression and pathological features of insulin-like growth factor-Ⅱ(IGF-Ⅱ)in tissues and sera of hepatocellular carcinoma(HCC) patients and the siRNA-mediated inhibition
文摘Objective To examine the expression of follistatinlike protein 1(FSTL1)in the peripheral blood and intestinal mucosa tissues of ulcerative colitis(UC)patients and analyze the correlation between its expression and the activity of UC.Methods From October 2010 to J une2012,sixty patients with UC were collected.From
文摘Objective: To observe the effects of leptin on glucose oxidation and glueokinase gene expression in rat liver cells. Methods: Rat liver cells were incubated with leptin of different doses (ranging from 10μg/L to 200μg/L). Control liver cells
文摘Objective To investigate the expression of survivin in leukemia and the prognostic significance in acute leukemia(AL). Methods The expression of survivin mRNA was measured in 105 AL and 21 chronic myelogenous leukemia (CML) patients with semi-quantity reverse transcription (RT)-PCR.15 adults were tested as
