[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus（AIV）.[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid（MPA） to be m...[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus（AIV）.[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid（MPA） to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H9 subtype AIV with N-ethy-N＇-（3-dimethyl aminopropyl）carbodiimide hydrochloride（EDC） and N-hydroxysuccinimide（NHS）.The immunosensor to detect H9 subtype AIV was established.[Result] The results showed that the immunosensor displayed better specificity to H9 AIV and had no response to H5AIV and NDV when it was used for detection.The sensitivity test indicated the detection sensitivity for the H9 subtype AIV could reach 20-100 EID50.[Conclusion] The research provided a foundation for further research on the immunosensor for detecting AIV and it could be a new approach to detect other related viruses.展开更多
A novel 2×5 model of insert-plug piezoelectric quartz crystal tumor marker micro-array immunosensor constructed with screw clamp apparatus has been developed for quantitative detection of the tumor markers such a...A novel 2×5 model of insert-plug piezoelectric quartz crystal tumor marker micro-array immunosensor constructed with screw clamp apparatus has been developed for quantitative detection of the tumor markers such as alpha-fetoprotein (AFP),carcino-embryonic antigen (CEA),prostate specific antigen (PSA),and human chorionic gonadotropin (hCG) in serum,in which every crystal unit can oscillate independently with the stability of±1 hertz (Hz) in air and±2 Hz in liquid.These response characteristics of Pz tumor marker micro-array immunosensor such as temperature, time-cost,reproducibility and specificity etc were also investigated.The detection ranges for AFP,CEA,PSA,and hCG obtained by Pz micro-array immunosensor were 20 ng/ml～640 ng/ml,1.56 ng/ml～50 ng/ml,1.25 ng/ml～50 ng/ml,and 2.5 mIU/ml～250 mlU/mi respectively with the coefficient of variance (CV) less than 5%.No cross-reactivates with other tumor markers in serum were observed.The results of AFP,CEA,PSA,and hCG obtained by this method from 68 serum samples were in good agreement with those given by chemiluminescence immunoassay with the correlation coefficients of 0.92,0.90,0.91,and 0.94 respectively.The Pz immunosensor regenerated by urea solution could be reused for five times without appreciable loss of response activity.Therefore,the proposed insert-plug immunosensor provides a rapid, sensitive,specific,reusable,convenient and reliable alternative for the detection of tumor markers in clinical laboratory.展开更多
A piezoelectric immunosensor has been developed for the detection of complement C4. Anti-C4 antibody was immobilized onto the gold electrodes of a 9 MHz AT-cut piezoelectric crystal. The coated crystal with the physic...A piezoelectric immunosensor has been developed for the detection of complement C4. Anti-C4 antibody was immobilized onto the gold electrodes of a 9 MHz AT-cut piezoelectric crystal. The coated crystal with the physical adsorption method to immobilize antibody showed the better results than the polyethyleneimine adhesion, glutaraldehyde cross-linking method with respect to sensitivity and reproducibility. The antibody-bound crystal with the physical adsorption method was successfully used for the detection of human complement C4 in the concentration range of 0.1—10 μg/mL for 40 min incubation time. The immunosensor system had good selectivity, and other materials in human Serum did not interfere the detection remarkably. The crystal could be regenerated nearly 15 times when the bound materials on the crystal surface were eluted by strong acid and strong alkali solutions and subsequently cleaned in a ultrasonic cleaner.展开更多
基金Supported by the Supporting Program of the"Eleventh Five-year Plan"for Sci&Tech Research of China(2006BAK20A29)Strategical Project for Science and Technology of Guangdong Province(2004A2090102)~~
文摘[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus（AIV）.[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid（MPA） to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H9 subtype AIV with N-ethy-N＇-（3-dimethyl aminopropyl）carbodiimide hydrochloride（EDC） and N-hydroxysuccinimide（NHS）.The immunosensor to detect H9 subtype AIV was established.[Result] The results showed that the immunosensor displayed better specificity to H9 AIV and had no response to H5AIV and NDV when it was used for detection.The sensitivity test indicated the detection sensitivity for the H9 subtype AIV could reach 20-100 EID50.[Conclusion] The research provided a foundation for further research on the immunosensor for detecting AIV and it could be a new approach to detect other related viruses.
文摘A novel 2×5 model of insert-plug piezoelectric quartz crystal tumor marker micro-array immunosensor constructed with screw clamp apparatus has been developed for quantitative detection of the tumor markers such as alpha-fetoprotein (AFP),carcino-embryonic antigen (CEA),prostate specific antigen (PSA),and human chorionic gonadotropin (hCG) in serum,in which every crystal unit can oscillate independently with the stability of±1 hertz (Hz) in air and±2 Hz in liquid.These response characteristics of Pz tumor marker micro-array immunosensor such as temperature, time-cost,reproducibility and specificity etc were also investigated.The detection ranges for AFP,CEA,PSA,and hCG obtained by Pz micro-array immunosensor were 20 ng/ml～640 ng/ml,1.56 ng/ml～50 ng/ml,1.25 ng/ml～50 ng/ml,and 2.5 mIU/ml～250 mlU/mi respectively with the coefficient of variance (CV) less than 5%.No cross-reactivates with other tumor markers in serum were observed.The results of AFP,CEA,PSA,and hCG obtained by this method from 68 serum samples were in good agreement with those given by chemiluminescence immunoassay with the correlation coefficients of 0.92,0.90,0.91,and 0.94 respectively.The Pz immunosensor regenerated by urea solution could be reused for five times without appreciable loss of response activity.Therefore,the proposed insert-plug immunosensor provides a rapid, sensitive,specific,reusable,convenient and reliable alternative for the detection of tumor markers in clinical laboratory.
基金Project supported by the National Natural Science Foundation of China.
文摘A piezoelectric immunosensor has been developed for the detection of complement C4. Anti-C4 antibody was immobilized onto the gold electrodes of a 9 MHz AT-cut piezoelectric crystal. The coated crystal with the physical adsorption method to immobilize antibody showed the better results than the polyethyleneimine adhesion, glutaraldehyde cross-linking method with respect to sensitivity and reproducibility. The antibody-bound crystal with the physical adsorption method was successfully used for the detection of human complement C4 in the concentration range of 0.1—10 μg/mL for 40 min incubation time. The immunosensor system had good selectivity, and other materials in human Serum did not interfere the detection remarkably. The crystal could be regenerated nearly 15 times when the bound materials on the crystal surface were eluted by strong acid and strong alkali solutions and subsequently cleaned in a ultrasonic cleaner.
