摘要
A novel 2×5 model of insert-plug piezoelectric quartz crystal tumor marker micro-array immunosensor constructed with screw clamp apparatus has been developed for quantitative detection of the tumor markers such as alpha-fetoprotein (AFP),carcino-embryonic antigen (CEA),prostate specific antigen (PSA),and human chorionic gonadotropin (hCG) in serum,in which every crystal unit can oscillate independently with the stability of±1 hertz (Hz) in air and±2 Hz in liquid.These response characteristics of Pz tumor marker micro-array immunosensor such as temperature, time-cost,reproducibility and specificity etc were also investigated.The detection ranges for AFP,CEA,PSA,and hCG obtained by Pz micro-array immunosensor were 20 ng/ml～640 ng/ml,1.56 ng/ml～50 ng/ml,1.25 ng/ml～50 ng/ml,and 2.5 mIU/ml～250 mlU/mi respectively with the coefficient of variance (CV) less than 5%.No cross-reactivates with other tumor markers in serum were observed.The results of AFP,CEA,PSA,and hCG obtained by this method from 68 serum samples were in good agreement with those given by chemiluminescence immunoassay with the correlation coefficients of 0.92,0.90,0.91,and 0.94 respectively.The Pz immunosensor regenerated by urea solution could be reused for five times without appreciable loss of response activity.Therefore,the proposed insert-plug immunosensor provides a rapid, sensitive,specific,reusable,convenient and reliable alternative for the detection of tumor markers in clinical laboratory.
A novel 2× 5 model of insert-plug piezoelectric quartz crystal tumor marker micro-array immunosensor constructed with screw clamp apparatus has been developed for quantitative detection of the tumor markers such as alpha-fetoprotein （AFP）, carcino-embryonic antigen （CEA）, prostate specific antigen （PSA）, and human chorionic gonadotropin （hCG） in serum, in which every crystal unit can oscillate independently with the stability of ± 1 hertz （Hz） in air and ＋2 Hz in liquid. These response characteristics of Pz tumor marker micro-array immunosensor such as temperature, time-cost, reproducibility and specificity etc were also investigated. The detection ranges for AFP, CEA, PSA, and hCG obtained by Pz micro-array immunosensor were 20 ng/ml-640 ng/ml, 1.56 ng/ml-50 ng/ml, 1.25 ng/ml-50 ng/ml, and 2.5 mIU/ml-250 mIU/ml respectively with the coefficient of variance （CV） less than 5%. No cross-reactivates with other tumor markers in serum were observed. The results of AFP, CEA, PSA, and hCG obtained by this method from 68 serum samples were in good agreement with those given by chemiluminescence immunoassay with the correlation coefficients of 0.92, 0.90, 0.91, and 0.94 respectively. The Pz immunosensor regenerated by urea solution could be reused for five times without appreciable loss of response activity. Therefore, the proposed insert-plug immunosensor provides a rapid, sensitive, specific, reusable, convenient and reliable alternative for the detection of tumor markers in clinical laboratory.
出处
《稀有金属材料与工程》
SCIE
EI
CAS
CSCD
北大核心
2006年第A03期405-408,共4页
Rare Metal Materials and Engineering
