Cotton production faces significant challenges from insect pests,with chemical pesticide use becoming increasingly limited by resistance and environmental concerns.This study explores the potential use of caffeine,a n...Cotton production faces significant challenges from insect pests,with chemical pesticide use becoming increasingly limited by resistance and environmental concerns.This study explores the potential use of caffeine,a natural plant alkaloid,as an environmentally friendly insect resistance strategy in cotton.Exogenous caffeine application demonstrated potent insecticidal effects against cotton bollworm(Helicoverpa armigera)larvae,with concentrations≥2 mg mL−1 causing near-complete feeding cessation and up to 70%larval mortality.Building on this,we engineered transgenic cotton(Gossypium hirsutum cv.Jin668)for heterologous caffeine biosynthesis by introducing three key N-methyltransferase genes(CaXMT1,CaMXMT1,CaDXMT1)by multiple gene transformation.Transgenic lines expressing all three genes showed remarkable caffeine accumulation(up to 3.59 mg g−1 dry weight),whereas two-gene combinations exhibited wild-type-level production.Feeding preference assays revealed that caffeine-enriched cotton strongly deterred feeding by H.armigera.Non-choice feeding trials demonstrated reduced leaf consumption and reduced larval growth in H.armigera fed on caffeine-producing cotton.The study highlights the effectiveness of synthetic biology approaches using the TGSII-UNiE multigene stacking system,despite challenges in transgene stability.This work advances plant-derived insect resistance research and provides a sustainable framework for reducing chemical pesticide reliance in cotton production,while underscoring unique potential of cotton as a synthetic biology platform for secondary metabolite engineering.展开更多
THE Torch Festival is the most significant and grandest celebration for all Yi communities across China’s Yunnan,Guizhou,and Sichuan provinces,with a history spanning over a thousand years.It reflects the Yi people’...THE Torch Festival is the most significant and grandest celebration for all Yi communities across China’s Yunnan,Guizhou,and Sichuan provinces,with a history spanning over a thousand years.It reflects the Yi people’s long-standing reverence for fire.Originating from the Yi ancestors’worship of fire,the festival was intended to drive away pests and evil spirits,protect crops,and represent prayers for a bountiful harvest.展开更多
The Leafminers,representing a diverse group of insects from various genera within the Agromyzidae family,pose a significant threat to spinach(Spinacia oleracea L.)production.This study aimed to identify single nucleot...The Leafminers,representing a diverse group of insects from various genera within the Agromyzidae family,pose a significant threat to spinach(Spinacia oleracea L.)production.This study aimed to identify single nucleotide polymorphism(SNP)markers associated with leafminer resistance through a genome-wide association study(GWAS)and to evaluate the prediction accuracy(PA)for selecting resistant spinach using genomic prediction(GP).Using a dataset of 84301 SNPs obtained from whole-genome resequencing,seven GWAS models,including BLINK,FarmCPU,MLM,and MLMM in GAPIT 3,as well as MLM,GLM,and SMR in TASSEL 5,were employed to perform GWAS on a panel of 286 USDA spinach germplasm accessions.Three SNP markers,namely 1_115279256_C_T,3_157082529_C_T,and 4_168510908_T_G on chromosomes 1,3,and 4,respectively,were identified as associated with leafminer resistance.In the 30 kb flanking regions of these markers,four candidate genes(SOV1g031330,SOV1g031340,SOV4g047270,and SOV4g047280),encoding LOB domain-containing protein,KH domain-containing protein,were discovered.Nodulin-like domain-containing protein,and SAM domain-containing protein,were discovered.The PA for leafminer resistance selection was estimated using ten different SNP sets,including two GWAS-derived marker sets(three and 51 SNPs)and eight random marker sets(ranging from 51 to 10 K SNPs)analyzed by seven GP models.The findings emphasized the superior performance of GWAS-derived SNP sets,reaching a PA of up to 0.79 using the cBLUP model.Notably,this research marks the pioneering application of GP in the context of insect resistance,providing a significant advancement in the understanding and management of leafminer resistance in spinach cultivation.展开更多
基金supported by the National Natural Science Foundation of China (32325039)
文摘Cotton production faces significant challenges from insect pests,with chemical pesticide use becoming increasingly limited by resistance and environmental concerns.This study explores the potential use of caffeine,a natural plant alkaloid,as an environmentally friendly insect resistance strategy in cotton.Exogenous caffeine application demonstrated potent insecticidal effects against cotton bollworm(Helicoverpa armigera)larvae,with concentrations≥2 mg mL−1 causing near-complete feeding cessation and up to 70%larval mortality.Building on this,we engineered transgenic cotton(Gossypium hirsutum cv.Jin668)for heterologous caffeine biosynthesis by introducing three key N-methyltransferase genes(CaXMT1,CaMXMT1,CaDXMT1)by multiple gene transformation.Transgenic lines expressing all three genes showed remarkable caffeine accumulation(up to 3.59 mg g−1 dry weight),whereas two-gene combinations exhibited wild-type-level production.Feeding preference assays revealed that caffeine-enriched cotton strongly deterred feeding by H.armigera.Non-choice feeding trials demonstrated reduced leaf consumption and reduced larval growth in H.armigera fed on caffeine-producing cotton.The study highlights the effectiveness of synthetic biology approaches using the TGSII-UNiE multigene stacking system,despite challenges in transgene stability.This work advances plant-derived insect resistance research and provides a sustainable framework for reducing chemical pesticide reliance in cotton production,while underscoring unique potential of cotton as a synthetic biology platform for secondary metabolite engineering.
文摘THE Torch Festival is the most significant and grandest celebration for all Yi communities across China’s Yunnan,Guizhou,and Sichuan provinces,with a history spanning over a thousand years.It reflects the Yi people’s long-standing reverence for fire.Originating from the Yi ancestors’worship of fire,the festival was intended to drive away pests and evil spirits,protect crops,and represent prayers for a bountiful harvest.
基金supported by USDA-SCRI(Grant Nos.2017-51181-26830 and 2023-51181-41321)USDA-AMS SCMP(Grant No.16SCCMAR0001)+1 种基金Arkansas Department of Agriculture SCBGP(Grant No.AM22SCBGPAR1130-00)USDA NIFA Hatch project ARK0VG2018 and ARK02440.
文摘The Leafminers,representing a diverse group of insects from various genera within the Agromyzidae family,pose a significant threat to spinach(Spinacia oleracea L.)production.This study aimed to identify single nucleotide polymorphism(SNP)markers associated with leafminer resistance through a genome-wide association study(GWAS)and to evaluate the prediction accuracy(PA)for selecting resistant spinach using genomic prediction(GP).Using a dataset of 84301 SNPs obtained from whole-genome resequencing,seven GWAS models,including BLINK,FarmCPU,MLM,and MLMM in GAPIT 3,as well as MLM,GLM,and SMR in TASSEL 5,were employed to perform GWAS on a panel of 286 USDA spinach germplasm accessions.Three SNP markers,namely 1_115279256_C_T,3_157082529_C_T,and 4_168510908_T_G on chromosomes 1,3,and 4,respectively,were identified as associated with leafminer resistance.In the 30 kb flanking regions of these markers,four candidate genes(SOV1g031330,SOV1g031340,SOV4g047270,and SOV4g047280),encoding LOB domain-containing protein,KH domain-containing protein,were discovered.Nodulin-like domain-containing protein,and SAM domain-containing protein,were discovered.The PA for leafminer resistance selection was estimated using ten different SNP sets,including two GWAS-derived marker sets(three and 51 SNPs)and eight random marker sets(ranging from 51 to 10 K SNPs)analyzed by seven GP models.The findings emphasized the superior performance of GWAS-derived SNP sets,reaching a PA of up to 0.79 using the cBLUP model.Notably,this research marks the pioneering application of GP in the context of insect resistance,providing a significant advancement in the understanding and management of leafminer resistance in spinach cultivation.
