Acetogenins are lipidic polyketides with antioxidant,antimicrobial,cytotoxic,and antitumor properties,mainly found in the roots,stems,bark,leaves,and fruits(particularly the seeds)of Annonaceae species.Previous studie...Acetogenins are lipidic polyketides with antioxidant,antimicrobial,cytotoxic,and antitumor properties,mainly found in the roots,stems,bark,leaves,and fruits(particularly the seeds)of Annonaceae species.Previous studies have identified acetogenins in the peel and pulp of soursop(Annona muricata L.)fruits.In this research,acetogenins present in starch and pectin extracted from these fruits were analyzed and identified,given their potential importance in the pharmaceutical and possibly in the food industries.The objective was to identify and quantify acetogenins in starch and pectin of soursop fruits.Extraction of both polysaccharides was performed using conventional and ultrasound-assisted methods,obtaining two types of pastes(white and brown).These were characterized by Fourier Transform Infrared Spectroscopy(FTIR).Methanolic extracts from the pastes were analyzed by thin-layer chromatography and open-column fractionation.Acetogenins were identified and quantified by high-performance liquid chromatography(HPLC).The compounds identified were pseudoannonacin and anonacin.In pectin,the concentration(μg g^(−1)of dry basis P/A)of pseudoannonacin was 340.06(conventional)and 452.80(ultrasound-assisted),while for anonacin it was 302.29(conventional)and 397.23(ultrasound-assisted).In starch,the concentration(μg g^(−1)of dry basis P/A)of pseudoannonacin was 20.72(conventional)and 21.60(ultrasound-assisted),whereas anonacin showed concentrations of 1.63(conventional)and 3.12(ultrasound-assisted).These results confirm the presence of anonacin and pseudoannonacin in both starch and pectin of soursop fruits,with pseudoannonacin being the most abundant acetogenin detected.展开更多
Tropomyosin(TM)is the predominant allergenic protein in shrimp,which has induced IgE-mediated food allergy reactions.In this study,Lit v 1 was purified from Penaeus vannamei muscles,which were glyco sylated by pectic ...Tropomyosin(TM)is the predominant allergenic protein in shrimp,which has induced IgE-mediated food allergy reactions.In this study,Lit v 1 was purified from Penaeus vannamei muscles,which were glyco sylated by pectic oligosaccharides.The results showed that di-galacturonic acid(DGA)and galacturonic acid(GA)induced the unfolding of the primary protein structure of TM,and changedα-helical structure,and IgE binding capacity.In addition,compared with TM-sensitized BALB/c mice,GA-modified TM(GA-TM)and DGA-modified TM(DGA-TM)were insufficient to stimulate sensitization,and significantly reduced the hypersecretion of IgE,IgG1,HIS,and IL-4,up-regulated IgG2a and IFN-y levels to improve imbalanced Th1/Th2,Treg/Th17 immunity,and promoted mRNA expression of tight junction proteins.Together,this study confirmed that glycosylation modification alleviated sensitization by altering TM structure,reducing IgE binding allergic inflammatory response,and regulating cytokine immune balance.Overall,these results indicated that glycosylation modification was a promising method for decreasing the allergenic reactivity of allergic proteins.展开更多
Pectin is a natural polysaccharide with a complex structure consisting of linear and branched regions rich in galacturonic acid.The growing interest in orange peel pectin can be attributed to its abundant supply.Accor...Pectin is a natural polysaccharide with a complex structure consisting of linear and branched regions rich in galacturonic acid.The growing interest in orange peel pectin can be attributed to its abundant supply.According to statistics,about 10 million tons of orange peel waste are produced worldwide each year.Traditionally,the extraction and utilization of pectin have focused on its gelling,thickening,and stabilizing properties in food.However,as more and more research teams have found that pectin has good biocompatibility,biodegradability and easy chemical modification,its potential in drug delivery systems,tissue engineering,and wound healing is gradually being explored.This review focuses on orange peel pectin polysaccharides and discusses its traditional and modern extraction techniques,especially the advanced method of subcritical water extraction.This study also outlines the structural modifications of pectin such as methylation and acetylation,and introduces its antioxidant and anticancer biological activities and their emerging roles in the development of advanced biomaterials such as bone tissue engineering and fibre pad dressings.展开更多
We studied the effect of two independent variables, the pectin/calcium chloride weight ratio and the overall matrix weight in HPMC/pectin/calcium matrix tablet, on the release of indomethacin. A two-factor 5-level cen...We studied the effect of two independent variables, the pectin/calcium chloride weight ratio and the overall matrix weight in HPMC/pectin/calcium matrix tablet, on the release of indomethacin. A two-factor 5-level central composite experimental design was employed. Responses of the Peppas correlation parameters n and K and the 10% release time （T0.1） were optimized by response surface methodology. Significant effect of the independent variables on the biphasic release parameters, n and K, was observed. N, K and T0.1 were well fitted with the second-order quadratic equations rather than linear equations. Moreover, the mathematic fitting and the response surfaces showed significant cross-interaction between the pectin/calcium chloride ratio and the overall matrix weight. The optimal formulation with larger n, longer T0.1 and smaller K consisted of medium pectin/calcium chloride ratio around 1.0 and medium matrix weight around 200 mg. Validation studies on the optimal formulations showed good predictability of the n, K and T0.1 values with biases within the range of-7.33% and 6.26%. Our results support that central composite design can be used to optimize drug release from HPMC/pectin/calcium matrix tablet with high predictability.展开更多
基金funded by the Sectoral Fund for Research in Agricultural,Livestock,Aquaculture,Agrobiotechnology,and Plant Genetic Resources(SADER-SECIHTI)Number 266891the Sectoral Fund for Research for Education(SEP-SECIHTI)Number 242718.
文摘Acetogenins are lipidic polyketides with antioxidant,antimicrobial,cytotoxic,and antitumor properties,mainly found in the roots,stems,bark,leaves,and fruits(particularly the seeds)of Annonaceae species.Previous studies have identified acetogenins in the peel and pulp of soursop(Annona muricata L.)fruits.In this research,acetogenins present in starch and pectin extracted from these fruits were analyzed and identified,given their potential importance in the pharmaceutical and possibly in the food industries.The objective was to identify and quantify acetogenins in starch and pectin of soursop fruits.Extraction of both polysaccharides was performed using conventional and ultrasound-assisted methods,obtaining two types of pastes(white and brown).These were characterized by Fourier Transform Infrared Spectroscopy(FTIR).Methanolic extracts from the pastes were analyzed by thin-layer chromatography and open-column fractionation.Acetogenins were identified and quantified by high-performance liquid chromatography(HPLC).The compounds identified were pseudoannonacin and anonacin.In pectin,the concentration(μg g^(−1)of dry basis P/A)of pseudoannonacin was 340.06(conventional)and 452.80(ultrasound-assisted),while for anonacin it was 302.29(conventional)and 397.23(ultrasound-assisted).In starch,the concentration(μg g^(−1)of dry basis P/A)of pseudoannonacin was 20.72(conventional)and 21.60(ultrasound-assisted),whereas anonacin showed concentrations of 1.63(conventional)and 3.12(ultrasound-assisted).These results confirm the presence of anonacin and pseudoannonacin in both starch and pectin of soursop fruits,with pseudoannonacin being the most abundant acetogenin detected.
基金supported by the National Natural Science Fund(31601395)Central Guidance on Local Science and Technology Development Fund of Guizhou Province([2023]016)+1 种基金China National Center for Food Safety Risk Assessment(2018K01-20171211)Lueyang Black-Bone Chicken Industry Development Research Institute(WJYJY-2021-9)。
文摘Tropomyosin(TM)is the predominant allergenic protein in shrimp,which has induced IgE-mediated food allergy reactions.In this study,Lit v 1 was purified from Penaeus vannamei muscles,which were glyco sylated by pectic oligosaccharides.The results showed that di-galacturonic acid(DGA)and galacturonic acid(GA)induced the unfolding of the primary protein structure of TM,and changedα-helical structure,and IgE binding capacity.In addition,compared with TM-sensitized BALB/c mice,GA-modified TM(GA-TM)and DGA-modified TM(DGA-TM)were insufficient to stimulate sensitization,and significantly reduced the hypersecretion of IgE,IgG1,HIS,and IL-4,up-regulated IgG2a and IFN-y levels to improve imbalanced Th1/Th2,Treg/Th17 immunity,and promoted mRNA expression of tight junction proteins.Together,this study confirmed that glycosylation modification alleviated sensitization by altering TM structure,reducing IgE binding allergic inflammatory response,and regulating cytokine immune balance.Overall,these results indicated that glycosylation modification was a promising method for decreasing the allergenic reactivity of allergic proteins.
文摘Pectin is a natural polysaccharide with a complex structure consisting of linear and branched regions rich in galacturonic acid.The growing interest in orange peel pectin can be attributed to its abundant supply.According to statistics,about 10 million tons of orange peel waste are produced worldwide each year.Traditionally,the extraction and utilization of pectin have focused on its gelling,thickening,and stabilizing properties in food.However,as more and more research teams have found that pectin has good biocompatibility,biodegradability and easy chemical modification,its potential in drug delivery systems,tissue engineering,and wound healing is gradually being explored.This review focuses on orange peel pectin polysaccharides and discusses its traditional and modern extraction techniques,especially the advanced method of subcritical water extraction.This study also outlines the structural modifications of pectin such as methylation and acetylation,and introduces its antioxidant and anticancer biological activities and their emerging roles in the development of advanced biomaterials such as bone tissue engineering and fibre pad dressings.
基金Shanghai Municipal Committee of Sciencc and Tcchnology (Grant No.024319114).
文摘We studied the effect of two independent variables, the pectin/calcium chloride weight ratio and the overall matrix weight in HPMC/pectin/calcium matrix tablet, on the release of indomethacin. A two-factor 5-level central composite experimental design was employed. Responses of the Peppas correlation parameters n and K and the 10% release time （T0.1） were optimized by response surface methodology. Significant effect of the independent variables on the biphasic release parameters, n and K, was observed. N, K and T0.1 were well fitted with the second-order quadratic equations rather than linear equations. Moreover, the mathematic fitting and the response surfaces showed significant cross-interaction between the pectin/calcium chloride ratio and the overall matrix weight. The optimal formulation with larger n, longer T0.1 and smaller K consisted of medium pectin/calcium chloride ratio around 1.0 and medium matrix weight around 200 mg. Validation studies on the optimal formulations showed good predictability of the n, K and T0.1 values with biases within the range of-7.33% and 6.26%. Our results support that central composite design can be used to optimize drug release from HPMC/pectin/calcium matrix tablet with high predictability.
