The ion coordination affinities of the commonly found metal ions were evaluated using DFT calculations.The results indicate that the lowest unoccupied molecular orbital(LUMO)energy of metal ions correlates positively ...The ion coordination affinities of the commonly found metal ions were evaluated using DFT calculations.The results indicate that the lowest unoccupied molecular orbital(LUMO)energy of metal ions correlates positively with their binding energies with O(S)ligands,and some metal ions with various valence states also present different affinities.Besides,due to the steric hindrance effects,the mono-and hexa-coordinated metal ions may exhibit different affinities,and the majority of the studied hexa-coordinated metal ions exhibit oxophilicity.These affinity differences perfectly illustrate the activation flotation practice in which the oxyphilic ions are applied to activating oxide minerals,while thiophilic ions are applied to activating sulfide minerals.展开更多
The ecological and evolutionary mechanisms underlying montane biodiversity patterns remain unresolved.To understand which factors determined community assembly rules in mountains,biogeographic affinity that represents...The ecological and evolutionary mechanisms underlying montane biodiversity patterns remain unresolved.To understand which factors determined community assembly rules in mountains,biogeographic affinity that represents the biogeographic and evolutionary history of species should incorporate with current environments.We aim to address two following questions:1)How does plant taxonomic and phylogenetic diversity with disparate biogeographic affinitiesvary along the subtropical elevational gradient?2)How do biogeographic affinityand environmental drivers regulate the community assembly?We collected woody plant survey data of 32 forest plots in a subtropical mountain of Mt.Guanshan with typical transitional characteristics,including 250 woody plant species belonging to 56 families and 118 genera.We estimated the effects of biogeographic affinity,climate and soil properties on taxonomic and phylogenetic diversity of plant communities employing linear regression and structural equation models.We found that the richness of temperate-affiliated species increased with elevations,but the evenness decreased,while tropical-affiliatedspecies had no significantpatterns.Winter temperature directly or indirectly via biogeographic affinityshaped the assemblage of woody plant communities along elevations.Biogeographic affinityaffected what kind of species could colonize higher elevations while local environment determined their fitnessto adapt.These results suggest that biogeographic affinityand local environment jointly lead to the dominance of temperate-affiliated species at higher elevations and shape the diversity of woody plant communities along elevational gradients.Our findingshighlight the legacy effect of biogeographic affinityon the composition and structure of subtropical montane forests.展开更多
In addition to the electron transfer,the appropriate H-adsorption affinity of active centers on the metal cocatalyst surface is quite important for high hydrogen-production activity of cocatalyst-modified photo-cataly...In addition to the electron transfer,the appropriate H-adsorption affinity of active centers on the metal cocatalyst surface is quite important for high hydrogen-production activity of cocatalyst-modified photo-catalysts.The typical Cu and Ru metal cocatalysts clearly exhibit a weak Cu-H bond and a strong Ru-H bond,respectively,resulting in limited activity for photocatalytic H_(2)evolution.In this work,an ingenious strategy of self-optimized H-adsorption affinity in CuRu alloy cocatalyst is developed to simultaneously reinforce the Cu-H bond and weaken the Ru-H bond by the intrinsic electron transfer from Cu to Ru atom.The CuRu alloy nanoparticles(2-3 nm)were deposited on the TiO_(2)surface to prepare CuRu/TiO_(2)through a one-step photoreduction method.Photocatalytic tests exhibited that the highest H_(2)-production rate of CuRu/TiO_(2)photocatalyst reached up to 5.316 mmol h^(-1)g^(-1),which was 24.80,1.86,and 2.60 times higher than that of the TiO_(2),Cu/TiO_(2),and Ru/TiO_(2),respectively.Based on the characterization results and theoretical calculations,the CuRu alloy cocatalyst exhibits excellent self-optimized H-adsorption affinity via the spontaneous electron transfer from Cu to Ru atom,which can greatly accelerate the photocatalytic H_(2)-production rate of TiO_(2).This work provides a feasible idea for the self-optimized H-adsorption affinity of metal active sites in the photocatalytic H_(2)-generation field.展开更多
Dynamic tracking analysis of monoclonal antibodies(mAbs)biotransformation in vivo is crucial,as certain modifications could inactivate the protein and reduce drug efficacy.However,a particular challenge(i.e.immune rec...Dynamic tracking analysis of monoclonal antibodies(mAbs)biotransformation in vivo is crucial,as certain modifications could inactivate the protein and reduce drug efficacy.However,a particular challenge(i.e.immune recognition deficiencies)in biotransformation studies may arise when modifications occur at the paratope recognized by the antigen.To address this limitation,a multi-epitope affinity technology utilizing the metal organic framework(MOF)@Au@peptide@aptamer composite material was proposed and developed by simultaneously immobilizing complementarity determining region(CDR)mimotope peptide(HH24)and non-CDR mimotope aptamer(CH1S-6T)onto the surface of MOF@Au nanocomposite.Comparative studies demonstrated that MOF@Au@peptide@aptamer exhibited significantly enhanced enrichment capabilities for trastuzumab variants in comparison to mono-epitope affinity technology.Moreover,the higher deamidation ratio for LC-Asn-30 and isomerization ratio for HCAsn-55 can only be monitored by the novel bioanalytical platform based on MOF@Au@peptide@aptamer and liquid chromatography-quadrupole time of flight-mass spectrometry(LC-QTOF-MS).Therefore,multi-epitope affinity technology could effectively overcome the biases of traditional affinity materials for key sites modification analysis of mAb.Particularly,the novel bioanalytical platform can be successfully used for the tracking analysis of trastuzumab modifications in different biological fluids.Compared to the spiked phosphate buffer(PB)model,faster modification trends were monitored in the spiked serum and patients'sera due to the catalytic effect of plasma proteins and relevant proteases.Differences in peptide modification levels of trastuzumab in patients'sera were also monitored.In summary,the novel bioanalytical platform based on the multi-epitope affinity technology holds great potentials for in vivo biotransformation analysis of mAb,contributing to improved understanding and paving the way for future research and clinical applications.展开更多
This article aims to explore effective ways to enhance the affinity of ideological and political course teachers in universities.By analyzing the connotation of affinity,the factors that affect the affinity of ideolog...This article aims to explore effective ways to enhance the affinity of ideological and political course teachers in universities.By analyzing the connotation of affinity,the factors that affect the affinity of ideological and political course teachers are analyzed,and corresponding improvement strategies are proposed.Research suggests that strengthening the construction of teacher ethics and conduct,improving teaching skills,enhancing emotional engagement,and enhancing practical training are key paths to enhance the affinity of ideological and political course teachers.The implementation of these paths will help improve the teaching quality and effectiveness of ideological and political courses,and promote the comprehensive development of students.展开更多
纽结理论是拓扑学的一个重要分支,虚拟纽结理论是经典纽结理论的推广,对它的研究是通过一种图解理论来展开的。虚拟纽结多项式是一类以多项式表达的虚拟纽结不变量,例如Arrow多项式和Wriggle多项式。Affine index多项式是以虚拟纽结图...纽结理论是拓扑学的一个重要分支,虚拟纽结理论是经典纽结理论的推广,对它的研究是通过一种图解理论来展开的。虚拟纽结多项式是一类以多项式表达的虚拟纽结不变量,例如Arrow多项式和Wriggle多项式。Affine index多项式是以虚拟纽结图的整数标记定义的单变量多项式。本文主要计算一类特殊虚拟纽结的Affine index多项式。按照Cheng着色的规则,对虚拟纽结图的每一段弧进行整数标记,计算每个经典交叉点的指标值,进而得到这类特殊虚拟纽结的Affine index多项式的表达式。Knot theory is an important branch of topology. Virtual knot theory is a generalization of classical knot theory, and its research is carried out through a graphic theory. The virtual knot polynomial refers to a class of virtual knot invariant expressed by polynomials, such as the Arrow polynomial and the Wriggle polynomial. The affine index polynomial is a univariate polynomial defined by the integer label of a virtual knot graph. In this paper, we mainly calculate affine index polynomials for a special class of virtual knots. According to the rules of Cheng coloring, we will integer label each arc of the virtual knot graph and calculate the index value of each classical crossings, and then get the expression of the affine index polynomial of this special virtual knot.展开更多
The paper presents a two-layer,disturbance-resistant,and fault-tolerant affine formation maneuver control scheme that accomplishes the surrounding of a dynamic target with multiple underactuated Quadrotor Unmanned Aer...The paper presents a two-layer,disturbance-resistant,and fault-tolerant affine formation maneuver control scheme that accomplishes the surrounding of a dynamic target with multiple underactuated Quadrotor Unmanned Aerial Vehicles(QUAVs).This scheme mainly consists of predefinedtime estimators and fixed-time tracking controllers,with a hybrid Laplacian matrix describing the communication among these QUAVs.At the first layer,we devise predefined time estimators for leading and following QUAVs,enabling accurate estimation of desired information.In the second layer,we initially devise a fixed-time hybrid observer to estimate unknown disturbances and actuator faults.Fixedtime translational tracking controllers are then proposed,and the intermediary control input from these controllers is used to extract the desired attitude and angular velocities for the fixed-time rotational tracking controllers.We employ an exact tracking differentiator to handle variables that are challenging to differentiate directly.The paper includes a demonstration of the control system stability through mathematical proof,as well as the presentation of simulation results and comparative simulations.展开更多
The inherent challenges arising from variations in user-captured viewpoints and object orientation disparities in real-world scenarios pose significant difficulties in establishing robust correspondence relationships ...The inherent challenges arising from variations in user-captured viewpoints and object orientation disparities in real-world scenarios pose significant difficulties in establishing robust correspondence relationships between image pairs.Methods based on geometric transformation estimation usually perform affine transformation of the global image for viewpoint correction,which not only increases the time complexity but also generates a large number of redundant features.To solve this problem,this paper proposes an adaptive affine transformation model(AATM)to achieve robust image matching by dividing special regions with pixel information and employing feature extraction algorithms with different granularities.First,the input image is divided into significant and non-significant regions by an adaptive algorithm.Second,for the salient region,the feature point extraction is accelerated by optimizing the longitude angle sampling algorithm and constructing the affine invariant nonlinear scale space,introducing the Hessian integral image and box filter.Then,for the non-significant region of the weak texture scene through the uniform step sampling algorithm,a dense feature description can be obtained in the weak texture scenes,so that more robust features are extracted for both significant and non-significant regions.The results of extensive experiments on two datasets show that the AATM algorithm outperforms similar algorithms in terms of the number of correctly matched pairs,elapsed time,and root mean square error(RMSE),indicating that the AATM can obtain more robust matches in scenes with large angle tilting and scale transformations.展开更多
The tropical flora in southern China is represented mainly by three regional flora, i.e. southern Yunnan (southwestern China), southwestern Guangxi (southwestern China) and Hainan Island (southern China). The floristi...The tropical flora in southern China is represented mainly by three regional flora, i.e. southern Yunnan (southwestern China), southwestern Guangxi (southwestern China) and Hainan Island (southern China). The floristic composition of each is concisely enumerated. Their geographical elements at generic level are analyzed. Furthermore, a comparison of floristic similarities between southern Yunnan and Hainan Island and Malay Peninsula and Brunei of western Malesia is made, based on existing regional flora treatments. The following is concluded: (1) The flora of southern China consists mainly of tropical floristic elements which contribute about 60% at the family level and more than 80 % at the generic level of its total flora. The dominant distribution type of the flora of southern China at the generic level is the tropical Asian distribution pattern. This reveals that the flora of southern China is of tropical nature with a strong tropical Asian affinity; (2) Most of the dominant families from the flora of southern China are also dominant in the Malesian flora. The floristic similarities between the regional flora of southern China and the regional flora of western Malesia are over 70% at the family level and more than 50% at the generic level. This suggests that the tropical flora of southern China belongs to the Malesian floristic region; (3) Situated at the northern margin of tropical Asia, on the other hand it is also obvious that the flora of southern China comprises less strictly tropical elements compared to the Malesian flora, and is consequently only a marginal type of the latter; (4) The close affinity of the flora of southern China to the Malesian flora can be explained by the geological history of southeast Asia.展开更多
Recombinant E.coli JM109, containing pHZ1818 plasmid which included the fused gene encoding human interleukin 6(IL 6), expressed a fusion protein with glutathion S transferase(GST). The fusion protein existed both...Recombinant E.coli JM109, containing pHZ1818 plasmid which included the fused gene encoding human interleukin 6(IL 6), expressed a fusion protein with glutathion S transferase(GST). The fusion protein existed both in the supernatant and inside the bacterial cell,but the insoluble protein had no biological activity and could not be refolded. The rotative speed of the shaker and the temperature of induction were optimized to maximize the expression of the soluble fusion protein. From the supernatant of the cell sonicates Glutathion Sephrose 4B affinity column chromatography was employed to isolate the fusion protein which could be purified to >80 0 0 in a single step. The yield of soluble GST IL 6 was about 10 mg per liter culture. The GST was site specifically cloven by 6 hours of treatment with thrombin and from the thrombin digest mixture IL 6 was purified by Q high performance ion exchange chromatography. From 1 liter of E.coli culture 2 mg refined IL 6 was obtained. The purified IL 6 had a purity of more than 95 0 0 and a biological activity of 1.02×10 8 IU/mg.展开更多
A new biosensor platform was explored for detection of surfactant based on fluorescence changes from single strand DNA （ssDNA） and single-walled carbon nanotubes （SWNTs）. Thermodynamics assay was performed to valu...A new biosensor platform was explored for detection of surfactant based on fluorescence changes from single strand DNA （ssDNA） and single-walled carbon nanotubes （SWNTs）. Thermodynamics assay was performed to value the stability of probe. The affinities of SWNT to five common surfactants （SDS, DBS, Triton X-100, Tween-20 and Tween-80） were investigated by real-time fluorescence method. The effects of Mg^2＋ and pH on the fluorescence intensity of self-assembled quenched sensor were performed. The fluorescent emission spectra were used to measure the responses of self-assembled quenched fluorescent of ssDNA/SWNTs to different concentration surfactant（Triton X-100）. The FAM-DNA wrapped SWNTs probe was stable in a wide temperature range （5 ℃ to 80℃）. The binding strength of surfactants and single-stranded DNA （ssDNA） on SWNTs surfaces was shown as follows： Triton X-100〉DBS〉Tween-20〉Tween-80〉ssDNA〉SDS, and the optimized reaction conditions included pH 7.4 and 10 mmol/L Mg2＋. The fluorescence of FAM-ssDNA wrapped SWNTs was proportionally recovered as a result of adding different concentrations of Triton X- 100, which realizes the quantitative detection of Triton X- 100.展开更多
With the continuous progress on affinity peptide research, it has become more and more popular in pharmacology and medicine. lt is promising to study these viruses affinity peptide to treat infectious diseases. And th...With the continuous progress on affinity peptide research, it has become more and more popular in pharmacology and medicine. lt is promising to study these viruses affinity peptide to treat infectious diseases. And the analysis on the virus affinity peptide with high selectivity and high sensitivity could provide valuable means for disease detection, treatment as wel as the study on the molecular mechanism of virus affinity peptide. Therefore, we reviewed the bioinformatics pre-diction technologies of computer simulation, molecular docking and homology model-ing, as wel as the research method on analyzing and screening virus affinity pep-tide, such as Phage display technology.展开更多
Recently a new clustering algorithm called 'affinity propagation' (AP) has been proposed, which efficiently clustered sparsely related data by passing messages between data points. However, we want to cluster ...Recently a new clustering algorithm called 'affinity propagation' (AP) has been proposed, which efficiently clustered sparsely related data by passing messages between data points. However, we want to cluster large scale data where the similarities are not sparse in many cases. This paper presents two variants of AP for grouping large scale data with a dense similarity matrix. The local approach is partition affinity propagation (PAP) and the global method is landmark affinity propagation (LAP). PAP passes messages in the subsets of data first and then merges them as the number of initial step of iterations; it can effectively reduce the number of iterations of clustering. LAP passes messages between the landmark data points first and then clusters non-landmark data points; it is a large global approximation method to speed up clustering. Experiments are conducted on many datasets, such as random data points, manifold subspaces, images of faces and Chinese calligraphy, and the results demonstrate that the two ap-proaches are feasible and practicable.展开更多
To improve the interfacial affinity and antifouling properties of polyphenylsulfone(PPSU)membrane, nano CuO/g-C3 N4(g-CN) sheets were synthesized via facile calcination route as one pot synthesis method. The uniformly...To improve the interfacial affinity and antifouling properties of polyphenylsulfone(PPSU)membrane, nano CuO/g-C3 N4(g-CN) sheets were synthesized via facile calcination route as one pot synthesis method. The uniformly assembled nanohybrid fillers, CuO on g-CN sheets were confirmed by using XRD, TEM, EDX and FTIR analysis. The non-solvent induced phase inversion technique was used to fabricate the nanohybrid ultrafiltration(UF)membranes by doping different concentration(0.5–1 wt.%) of nano CuO/g-C_3 N_4(g-CN)sheets within the PPSU matrix. The results of contact angle, atomic force microscopy,energy-dispersive X-ray spectroscopy reveal that surface structure and physico-chemical properties of nanohybrid membrane plays lead role in solute interaction and rejection compared to bare membrane, M0. Furthermore, the interfacial affinity of membrane was explored in detail via surface free energy, spreading coefficient, wetting tension and reversible work of adhesion analysis. Nanohybrid UF membrane, with 0.5% of the filler(M1)displayed remarkable permeation flux of 202, 131 L/m2/hr for pure water and protein solution, respectively while maintaining a high protein rejection(96%). Moreover, the exceptional dispersion of the nanosheets in the polymer matrix enhanced FRR(79%) and decreased the overall resistance of M1 compared to the pristine membrane(M0). Overall results suggest that the incorporation of nano sheets is a facile modification technique which improves the comprehensive membrane performance and holds a great potential to be further explored for water treatment.展开更多
A fossil with Gnetum affinity was found in the Jianshangou Member （Barremian Age） of the Yixian Formation （Lower Cretaceous Epoch） of the Jehol Group in western Liaoning, northeastern China. The single fossil spec...A fossil with Gnetum affinity was found in the Jianshangou Member （Barremian Age） of the Yixian Formation （Lower Cretaceous Epoch） of the Jehol Group in western Liaoning, northeastern China. The single fossil specimen is represented by both elongate-cylindrical male spike strobiles which borne within a nodal bract of cauliflorous branch. The spike strobiles have apparent nodes, invisible internodes, and numerous verticillate involucral collars. The microsporangiate units within involucral collars are not seen. The male spike strobiles with verticillate involucral collars occur exclusively in Gnetum; hence, the fossil strobiles are attributed to a new taxon, Khitania columnispicata gen. ＆ sp. nov., being closely related to Gnetum. The general isotopic dating suggests an age of Barremian, ca. 125-122 million years （Myr） ago for the Jianshangou Member. The palaeoecological and palaeoclimatic inference based on the compositions of flora and fauna, and lithological characters of the fossil locality suggests that the fossil plants grew in a subtropical mesophytic forest and under a warmer climate. The remains of male spike strobiles are the first record of gnetalean macrofossil. It documents the evolution of the distinct gnetoid morphology and indicates a wider range of distribution of Gnetaceae in the Early Cretaceous than present day.展开更多
Attempts were made to develop dyes with high affinity for polylactide as an alternative to the existent commercial disperse dyes. The dyes synthesized according to the affinity concept of dye to polylactide exhibited ...Attempts were made to develop dyes with high affinity for polylactide as an alternative to the existent commercial disperse dyes. The dyes synthesized according to the affinity concept of dye to polylactide exhibited excellent dyeing properties on polylactide compared with the commercial disperse dyes.展开更多
基金financially supported by the National Natural Science Foundation of China(Nos.52074356,U20A20269)the Key Technology Research and Development Program,China(No.2022YFC2904503)+4 种基金the Special Fund for the Construction of Hunan Province,China(No.2023SK2061)the Science and Technology Innovation Program of Hunan Province,China(No.2022RC1183)the Changsha Science and Technology Project(Changsha Outstanding Innovative Youth Training Program),ChinaInnovation-driven Project of Central South University,China(No.2023CXQD002)Higher Education Discipline Innovation Project,China(No.B14034)。
文摘The ion coordination affinities of the commonly found metal ions were evaluated using DFT calculations.The results indicate that the lowest unoccupied molecular orbital(LUMO)energy of metal ions correlates positively with their binding energies with O(S)ligands,and some metal ions with various valence states also present different affinities.Besides,due to the steric hindrance effects,the mono-and hexa-coordinated metal ions may exhibit different affinities,and the majority of the studied hexa-coordinated metal ions exhibit oxophilicity.These affinity differences perfectly illustrate the activation flotation practice in which the oxyphilic ions are applied to activating oxide minerals,while thiophilic ions are applied to activating sulfide minerals.
基金financialsupport from the Jiangxi Natural Science Foundation(20242BAB25345)to Z.Z.the Innovation Program of Shanghai Municipal Education Commission(2023ZKZD36)to J.Z.
文摘The ecological and evolutionary mechanisms underlying montane biodiversity patterns remain unresolved.To understand which factors determined community assembly rules in mountains,biogeographic affinity that represents the biogeographic and evolutionary history of species should incorporate with current environments.We aim to address two following questions:1)How does plant taxonomic and phylogenetic diversity with disparate biogeographic affinitiesvary along the subtropical elevational gradient?2)How do biogeographic affinityand environmental drivers regulate the community assembly?We collected woody plant survey data of 32 forest plots in a subtropical mountain of Mt.Guanshan with typical transitional characteristics,including 250 woody plant species belonging to 56 families and 118 genera.We estimated the effects of biogeographic affinity,climate and soil properties on taxonomic and phylogenetic diversity of plant communities employing linear regression and structural equation models.We found that the richness of temperate-affiliated species increased with elevations,but the evenness decreased,while tropical-affiliatedspecies had no significantpatterns.Winter temperature directly or indirectly via biogeographic affinityshaped the assemblage of woody plant communities along elevations.Biogeographic affinityaffected what kind of species could colonize higher elevations while local environment determined their fitnessto adapt.These results suggest that biogeographic affinityand local environment jointly lead to the dominance of temperate-affiliated species at higher elevations and shape the diversity of woody plant communities along elevational gradients.Our findingshighlight the legacy effect of biogeographic affinityon the composition and structure of subtropical montane forests.
基金supported by the National Natural Science Foun-dation of China(Nos.22178275 and U22A20147)the Natural Science Foundation of Hubei Province of China(No.2022CFA001).
文摘In addition to the electron transfer,the appropriate H-adsorption affinity of active centers on the metal cocatalyst surface is quite important for high hydrogen-production activity of cocatalyst-modified photo-catalysts.The typical Cu and Ru metal cocatalysts clearly exhibit a weak Cu-H bond and a strong Ru-H bond,respectively,resulting in limited activity for photocatalytic H_(2)evolution.In this work,an ingenious strategy of self-optimized H-adsorption affinity in CuRu alloy cocatalyst is developed to simultaneously reinforce the Cu-H bond and weaken the Ru-H bond by the intrinsic electron transfer from Cu to Ru atom.The CuRu alloy nanoparticles(2-3 nm)were deposited on the TiO_(2)surface to prepare CuRu/TiO_(2)through a one-step photoreduction method.Photocatalytic tests exhibited that the highest H_(2)-production rate of CuRu/TiO_(2)photocatalyst reached up to 5.316 mmol h^(-1)g^(-1),which was 24.80,1.86,and 2.60 times higher than that of the TiO_(2),Cu/TiO_(2),and Ru/TiO_(2),respectively.Based on the characterization results and theoretical calculations,the CuRu alloy cocatalyst exhibits excellent self-optimized H-adsorption affinity via the spontaneous electron transfer from Cu to Ru atom,which can greatly accelerate the photocatalytic H_(2)-production rate of TiO_(2).This work provides a feasible idea for the self-optimized H-adsorption affinity of metal active sites in the photocatalytic H_(2)-generation field.
基金supported by the National Natural Science Foundation of China(Grant Nos.:82373829,82273893,and 82173773)the Natural Science Foundation of Guangdong Province,China(Grant Nos.:2021A1515220099,and 2022A1515011576)+1 种基金the High-End Foreign Experts Project,China(Grant No.:G2021199005L)the Science and Technology Program of Guangdong Provincial Medical Products Administration,China(Grant Nos.:2023TDZ11,and 2022ZDB04).
文摘Dynamic tracking analysis of monoclonal antibodies(mAbs)biotransformation in vivo is crucial,as certain modifications could inactivate the protein and reduce drug efficacy.However,a particular challenge(i.e.immune recognition deficiencies)in biotransformation studies may arise when modifications occur at the paratope recognized by the antigen.To address this limitation,a multi-epitope affinity technology utilizing the metal organic framework(MOF)@Au@peptide@aptamer composite material was proposed and developed by simultaneously immobilizing complementarity determining region(CDR)mimotope peptide(HH24)and non-CDR mimotope aptamer(CH1S-6T)onto the surface of MOF@Au nanocomposite.Comparative studies demonstrated that MOF@Au@peptide@aptamer exhibited significantly enhanced enrichment capabilities for trastuzumab variants in comparison to mono-epitope affinity technology.Moreover,the higher deamidation ratio for LC-Asn-30 and isomerization ratio for HCAsn-55 can only be monitored by the novel bioanalytical platform based on MOF@Au@peptide@aptamer and liquid chromatography-quadrupole time of flight-mass spectrometry(LC-QTOF-MS).Therefore,multi-epitope affinity technology could effectively overcome the biases of traditional affinity materials for key sites modification analysis of mAb.Particularly,the novel bioanalytical platform can be successfully used for the tracking analysis of trastuzumab modifications in different biological fluids.Compared to the spiked phosphate buffer(PB)model,faster modification trends were monitored in the spiked serum and patients'sera due to the catalytic effect of plasma proteins and relevant proteases.Differences in peptide modification levels of trastuzumab in patients'sera were also monitored.In summary,the novel bioanalytical platform based on the multi-epitope affinity technology holds great potentials for in vivo biotransformation analysis of mAb,contributing to improved understanding and paving the way for future research and clinical applications.
文摘This article aims to explore effective ways to enhance the affinity of ideological and political course teachers in universities.By analyzing the connotation of affinity,the factors that affect the affinity of ideological and political course teachers are analyzed,and corresponding improvement strategies are proposed.Research suggests that strengthening the construction of teacher ethics and conduct,improving teaching skills,enhancing emotional engagement,and enhancing practical training are key paths to enhance the affinity of ideological and political course teachers.The implementation of these paths will help improve the teaching quality and effectiveness of ideological and political courses,and promote the comprehensive development of students.
文摘纽结理论是拓扑学的一个重要分支,虚拟纽结理论是经典纽结理论的推广,对它的研究是通过一种图解理论来展开的。虚拟纽结多项式是一类以多项式表达的虚拟纽结不变量,例如Arrow多项式和Wriggle多项式。Affine index多项式是以虚拟纽结图的整数标记定义的单变量多项式。本文主要计算一类特殊虚拟纽结的Affine index多项式。按照Cheng着色的规则,对虚拟纽结图的每一段弧进行整数标记,计算每个经典交叉点的指标值,进而得到这类特殊虚拟纽结的Affine index多项式的表达式。Knot theory is an important branch of topology. Virtual knot theory is a generalization of classical knot theory, and its research is carried out through a graphic theory. The virtual knot polynomial refers to a class of virtual knot invariant expressed by polynomials, such as the Arrow polynomial and the Wriggle polynomial. The affine index polynomial is a univariate polynomial defined by the integer label of a virtual knot graph. In this paper, we mainly calculate affine index polynomials for a special class of virtual knots. According to the rules of Cheng coloring, we will integer label each arc of the virtual knot graph and calculate the index value of each classical crossings, and then get the expression of the affine index polynomial of this special virtual knot.
基金supported by Natural Science Basic Research Plan in Shaanxi Province of China(No.2023-JC-QN-0733)Guangdong Basic and Applied Basic Research Foundation,China(No.2022A1515110753)+2 种基金China Postdoctoral Science Foundation(No.2022M722583)China Industry-UniversityResearch Innovation Foundation(No.2022IT188)National Key Laboratory of Air-based Information Perception and Fusion and the Aeronautic Science Foundation of China(No.20220001068001)。
文摘The paper presents a two-layer,disturbance-resistant,and fault-tolerant affine formation maneuver control scheme that accomplishes the surrounding of a dynamic target with multiple underactuated Quadrotor Unmanned Aerial Vehicles(QUAVs).This scheme mainly consists of predefinedtime estimators and fixed-time tracking controllers,with a hybrid Laplacian matrix describing the communication among these QUAVs.At the first layer,we devise predefined time estimators for leading and following QUAVs,enabling accurate estimation of desired information.In the second layer,we initially devise a fixed-time hybrid observer to estimate unknown disturbances and actuator faults.Fixedtime translational tracking controllers are then proposed,and the intermediary control input from these controllers is used to extract the desired attitude and angular velocities for the fixed-time rotational tracking controllers.We employ an exact tracking differentiator to handle variables that are challenging to differentiate directly.The paper includes a demonstration of the control system stability through mathematical proof,as well as the presentation of simulation results and comparative simulations.
基金Supported by the National Natural Science Foundation of China(No.61971162,61771186)the Natural Science Foundation of Heilongjiang Province(No.PL2024 F023)+1 种基金the Fundamental Scientific Research Funds of Heilongjiang Province(No.2022-KYYWF-1050)the Open Research Fund of National Mobile Communications Research Laboratory in Southeast University(No.2023D07).
文摘The inherent challenges arising from variations in user-captured viewpoints and object orientation disparities in real-world scenarios pose significant difficulties in establishing robust correspondence relationships between image pairs.Methods based on geometric transformation estimation usually perform affine transformation of the global image for viewpoint correction,which not only increases the time complexity but also generates a large number of redundant features.To solve this problem,this paper proposes an adaptive affine transformation model(AATM)to achieve robust image matching by dividing special regions with pixel information and employing feature extraction algorithms with different granularities.First,the input image is divided into significant and non-significant regions by an adaptive algorithm.Second,for the salient region,the feature point extraction is accelerated by optimizing the longitude angle sampling algorithm and constructing the affine invariant nonlinear scale space,introducing the Hessian integral image and box filter.Then,for the non-significant region of the weak texture scene through the uniform step sampling algorithm,a dense feature description can be obtained in the weak texture scenes,so that more robust features are extracted for both significant and non-significant regions.The results of extensive experiments on two datasets show that the AATM algorithm outperforms similar algorithms in terms of the number of correctly matched pairs,elapsed time,and root mean square error(RMSE),indicating that the AATM can obtain more robust matches in scenes with large angle tilting and scale transformations.
文摘The tropical flora in southern China is represented mainly by three regional flora, i.e. southern Yunnan (southwestern China), southwestern Guangxi (southwestern China) and Hainan Island (southern China). The floristic composition of each is concisely enumerated. Their geographical elements at generic level are analyzed. Furthermore, a comparison of floristic similarities between southern Yunnan and Hainan Island and Malay Peninsula and Brunei of western Malesia is made, based on existing regional flora treatments. The following is concluded: (1) The flora of southern China consists mainly of tropical floristic elements which contribute about 60% at the family level and more than 80 % at the generic level of its total flora. The dominant distribution type of the flora of southern China at the generic level is the tropical Asian distribution pattern. This reveals that the flora of southern China is of tropical nature with a strong tropical Asian affinity; (2) Most of the dominant families from the flora of southern China are also dominant in the Malesian flora. The floristic similarities between the regional flora of southern China and the regional flora of western Malesia are over 70% at the family level and more than 50% at the generic level. This suggests that the tropical flora of southern China belongs to the Malesian floristic region; (3) Situated at the northern margin of tropical Asia, on the other hand it is also obvious that the flora of southern China comprises less strictly tropical elements compared to the Malesian flora, and is consequently only a marginal type of the latter; (4) The close affinity of the flora of southern China to the Malesian flora can be explained by the geological history of southeast Asia.
文摘Recombinant E.coli JM109, containing pHZ1818 plasmid which included the fused gene encoding human interleukin 6(IL 6), expressed a fusion protein with glutathion S transferase(GST). The fusion protein existed both in the supernatant and inside the bacterial cell,but the insoluble protein had no biological activity and could not be refolded. The rotative speed of the shaker and the temperature of induction were optimized to maximize the expression of the soluble fusion protein. From the supernatant of the cell sonicates Glutathion Sephrose 4B affinity column chromatography was employed to isolate the fusion protein which could be purified to >80 0 0 in a single step. The yield of soluble GST IL 6 was about 10 mg per liter culture. The GST was site specifically cloven by 6 hours of treatment with thrombin and from the thrombin digest mixture IL 6 was purified by Q high performance ion exchange chromatography. From 1 liter of E.coli culture 2 mg refined IL 6 was obtained. The purified IL 6 had a purity of more than 95 0 0 and a biological activity of 1.02×10 8 IU/mg.
基金Projects (21075032, 21005026, 21135001) supported by the National Natural Science Foundation of ChinaProject (llJJ5012) supported by Hunan Provincial Natural Science Foundation, China
文摘A new biosensor platform was explored for detection of surfactant based on fluorescence changes from single strand DNA （ssDNA） and single-walled carbon nanotubes （SWNTs）. Thermodynamics assay was performed to value the stability of probe. The affinities of SWNT to five common surfactants （SDS, DBS, Triton X-100, Tween-20 and Tween-80） were investigated by real-time fluorescence method. The effects of Mg^2＋ and pH on the fluorescence intensity of self-assembled quenched sensor were performed. The fluorescent emission spectra were used to measure the responses of self-assembled quenched fluorescent of ssDNA/SWNTs to different concentration surfactant（Triton X-100）. The FAM-DNA wrapped SWNTs probe was stable in a wide temperature range （5 ℃ to 80℃）. The binding strength of surfactants and single-stranded DNA （ssDNA） on SWNTs surfaces was shown as follows： Triton X-100〉DBS〉Tween-20〉Tween-80〉ssDNA〉SDS, and the optimized reaction conditions included pH 7.4 and 10 mmol/L Mg2＋. The fluorescence of FAM-ssDNA wrapped SWNTs was proportionally recovered as a result of adding different concentrations of Triton X- 100, which realizes the quantitative detection of Triton X- 100.
基金Supported by the Key Science and Technology Program of Henan Province(162102110136)the Science and Technology Fund for Outstanding Young People of Henan Academy of Agricultural Sciences(2016YQ28)~~
文摘With the continuous progress on affinity peptide research, it has become more and more popular in pharmacology and medicine. lt is promising to study these viruses affinity peptide to treat infectious diseases. And the analysis on the virus affinity peptide with high selectivity and high sensitivity could provide valuable means for disease detection, treatment as wel as the study on the molecular mechanism of virus affinity peptide. Therefore, we reviewed the bioinformatics pre-diction technologies of computer simulation, molecular docking and homology model-ing, as wel as the research method on analyzing and screening virus affinity pep-tide, such as Phage display technology.
基金the National Natural Science Foundation of China (Nos. 60533090 and 60603096)the National Hi-Tech Research and Development Program (863) of China (No. 2006AA010107)+2 种基金the Key Technology R&D Program of China (No. 2006BAH02A13-4)the Program for Changjiang Scholars and Innovative Research Team in University of China (No. IRT0652)the Cultivation Fund of the Key Scientific and Technical Innovation Project of MOE, China (No. 706033)
文摘Recently a new clustering algorithm called 'affinity propagation' (AP) has been proposed, which efficiently clustered sparsely related data by passing messages between data points. However, we want to cluster large scale data where the similarities are not sparse in many cases. This paper presents two variants of AP for grouping large scale data with a dense similarity matrix. The local approach is partition affinity propagation (PAP) and the global method is landmark affinity propagation (LAP). PAP passes messages in the subsets of data first and then merges them as the number of initial step of iterations; it can effectively reduce the number of iterations of clustering. LAP passes messages between the landmark data points first and then clusters non-landmark data points; it is a large global approximation method to speed up clustering. Experiments are conducted on many datasets, such as random data points, manifold subspaces, images of faces and Chinese calligraphy, and the results demonstrate that the two ap-proaches are feasible and practicable.
基金financial support from the Science and Engineering Research Board(SERB)under National Post-Doctoral Fellowship scheme(PDF/2017/000283)
文摘To improve the interfacial affinity and antifouling properties of polyphenylsulfone(PPSU)membrane, nano CuO/g-C3 N4(g-CN) sheets were synthesized via facile calcination route as one pot synthesis method. The uniformly assembled nanohybrid fillers, CuO on g-CN sheets were confirmed by using XRD, TEM, EDX and FTIR analysis. The non-solvent induced phase inversion technique was used to fabricate the nanohybrid ultrafiltration(UF)membranes by doping different concentration(0.5–1 wt.%) of nano CuO/g-C_3 N_4(g-CN)sheets within the PPSU matrix. The results of contact angle, atomic force microscopy,energy-dispersive X-ray spectroscopy reveal that surface structure and physico-chemical properties of nanohybrid membrane plays lead role in solute interaction and rejection compared to bare membrane, M0. Furthermore, the interfacial affinity of membrane was explored in detail via surface free energy, spreading coefficient, wetting tension and reversible work of adhesion analysis. Nanohybrid UF membrane, with 0.5% of the filler(M1)displayed remarkable permeation flux of 202, 131 L/m2/hr for pure water and protein solution, respectively while maintaining a high protein rejection(96%). Moreover, the exceptional dispersion of the nanosheets in the polymer matrix enhanced FRR(79%) and decreased the overall resistance of M1 compared to the pristine membrane(M0). Overall results suggest that the incorporation of nano sheets is a facile modification technique which improves the comprehensive membrane performance and holds a great potential to be further explored for water treatment.
基金supported by the pilot project of Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-114)National Natural Science Foundation of China (40632010)
文摘A fossil with Gnetum affinity was found in the Jianshangou Member （Barremian Age） of the Yixian Formation （Lower Cretaceous Epoch） of the Jehol Group in western Liaoning, northeastern China. The single fossil specimen is represented by both elongate-cylindrical male spike strobiles which borne within a nodal bract of cauliflorous branch. The spike strobiles have apparent nodes, invisible internodes, and numerous verticillate involucral collars. The microsporangiate units within involucral collars are not seen. The male spike strobiles with verticillate involucral collars occur exclusively in Gnetum; hence, the fossil strobiles are attributed to a new taxon, Khitania columnispicata gen. ＆ sp. nov., being closely related to Gnetum. The general isotopic dating suggests an age of Barremian, ca. 125-122 million years （Myr） ago for the Jianshangou Member. The palaeoecological and palaeoclimatic inference based on the compositions of flora and fauna, and lithological characters of the fossil locality suggests that the fossil plants grew in a subtropical mesophytic forest and under a warmer climate. The remains of male spike strobiles are the first record of gnetalean macrofossil. It documents the evolution of the distinct gnetoid morphology and indicates a wider range of distribution of Gnetaceae in the Early Cretaceous than present day.
文摘Attempts were made to develop dyes with high affinity for polylactide as an alternative to the existent commercial disperse dyes. The dyes synthesized according to the affinity concept of dye to polylactide exhibited excellent dyeing properties on polylactide compared with the commercial disperse dyes.
