Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explan...Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale.展开更多
The structure of tubeimoside V (1), a new cyclic bisdesmoside, isolated from tubers of Bolbostemma paniculatum (Tu Bei Mu), was established by means of 2D NMR spectral and chemical methods. Compound 1 has inter-saccha...The structure of tubeimoside V (1), a new cyclic bisdesmoside, isolated from tubers of Bolbostemma paniculatum (Tu Bei Mu), was established by means of 2D NMR spectral and chemical methods. Compound 1 has inter-saccharide chain bridging by a dicrotalic acid to form a unique macrocyclic structure.展开更多
Objective To study the triterpenoid saponin from bulbs of Bolbostemma paniculatum.Methods The compound was isolated by repeated silica gel chromatographies and its strcuture was elucidated on the basis of physico chem...Objective To study the triterpenoid saponin from bulbs of Bolbostemma paniculatum.Methods The compound was isolated by repeated silica gel chromatographies and its strcuture was elucidated on the basis of physico chemical property and spectral analysis.Results A novel triterpenoid saponin was isolated and determined as olean 12-en-28-oic acid,3-{[2-O-[6-O-[(3R)-4-carboxy-3-hydrox-y-3-methyl-1-oxobutyl]-β-D-glucopyranosyl]-β-D-glucopyranosyl]oxy}-2,16,23-trihydroxy-28-[2-O-α-L-rhamnose(1→2)-α-L-arabinopyranosyl]ester(Iφ.Conclusion Compound I is a novel compound named as dexylosyltubeimosideⅢ.展开更多
Cynanchum paniculatum(Bunge) Kitagawa is usually used as an herbal medicine for treating many diseases. Paeonol is the main active component, and its content is the key indicator for quality control of C. paniculatum....Cynanchum paniculatum(Bunge) Kitagawa is usually used as an herbal medicine for treating many diseases. Paeonol is the main active component, and its content is the key indicator for quality control of C. paniculatum. In the present study, we developed a rapid, accurate and precise method for quantitation of paeonol in C. paniculatum using 1 H NMR spectra. The deuterated solvent of methanol-d4 enabled satisfactory separation of the signals to be integrated in 1 H NMR spectrum. H-6(δ 7.78) of 1 H NMR spectrum of C. paniculatum was selected as the feature signal for quantitation, and trimesic acid(TMA) was selected as an internal standard. Validation of the quantitative method was performed in terms of linearity, specificity, repeatability and stability. This is the first time to report quantitative 1 H NMR(qHNMR) applied to determine the content of paeonol in C. paniculatum and showed a wider linearity range than the reported quantitation of paeonol in others. The simple extraction of paeonol from C. paniculatum was rapid and will prompt the application of the developed method. This work implied that qHNMR represented a feasible alternative to HPLC-based methods for quantitation of paeonol in C. paniculatum, and it was suitable for the quality control of C. paniculatum.展开更多
Two new C21 steroidal glycosides,paniculatumosides H and I,together with four known ones were isolated from the roots of Cynanchum paniculatum(Bge.)Kitag.Their structures were identifed by spectroscopic methods includ...Two new C21 steroidal glycosides,paniculatumosides H and I,together with four known ones were isolated from the roots of Cynanchum paniculatum(Bge.)Kitag.Their structures were identifed by spectroscopic methods including extensive 1D and 2D NMR techniques.All compounds were subjected to detect the anti-tobacco mosaic virus(TMV)activities and their cytotoxities against three human tumor cell lines(SMMC-7721,MDA-MB-231 and A549).The results showed that compounds 1 and 5 exhibited potent protective activities against TMV,while 2,4 and 6 had moderate efects on the SMMC7721 cancer cells viability.展开更多
Two new sterols were isolated from bulbs of Bolbostemma paniculatum (Maxim.) Franquet. Their structures were elucidated as stigmasta-7, 22, 25-triene-3-O-nonadecanoic acid ester (1) and stigmasta-7, 22, 25-triene-3-O-...Two new sterols were isolated from bulbs of Bolbostemma paniculatum (Maxim.) Franquet. Their structures were elucidated as stigmasta-7, 22, 25-triene-3-O-nonadecanoic acid ester (1) and stigmasta-7, 22, 25-triene-3-O-B-D- (6'-palmitoyl) glucopyranoside (2) by chemical and spectroscopic methods.展开更多
Background: A healthy normal skin is essential for a person’s physical and mental well being. It is an important aspect of their sexual attractiveness, a sense of well being and a sense of self confidence. Vitiligo i...Background: A healthy normal skin is essential for a person’s physical and mental well being. It is an important aspect of their sexual attractiveness, a sense of well being and a sense of self confidence. Vitiligo is an acquired depigmentation disorder of skin affecting 1% - 4% of the world population. Neither life threatening nor symptomatic (except that depigmented patches burn easily when exposed to the sun) the effects of vitiligo can be cosmetically and psychologically devastating. Because the disease is still not understood, there is a plethora of different treatments approaches, but they are largely unsatisfactory from patient’s perspective. Objective: To report the outcomes from oral use of an infusion of leaves of Solanum paniculatum L., Jacaranda brasiliensis and Sonchus oleraceus for treatment of vitiligo. Ethical issues: Study approved by the Research Ethics Committee of Hospital Federal dos Servidores do Estado do Rio de Janeiro (CAAE: 32143314.9.0000.5252). All participants confirmed the condition of volunteer by signing the Informed Consent Form. Methods: From October 2014 to October 2015 twelve subjects consumed daily four glasses (800 ml) of proposed phytotherapeutic preparation. Results: All subjects were highly satisfied about their outcomes. Total depigmentation was considered as a positive outcome because it is cosmetically acceptable. Total depigmentation was observed in presence of 80% or more of depigmenteted patches. Conclusions: The phytotherapeutic preparation proposed is effective on treatment of depigmenteted patches in vitiligo.展开更多
From Combretum paniculatum flowers, two diglucosylated derivatives from cyanidin and pelargonidin were identified using chromatographic (TLC), chemical (degradation by hydrolysis, tests of revelations) and spectral [U...From Combretum paniculatum flowers, two diglucosylated derivatives from cyanidin and pelargonidin were identified using chromatographic (TLC), chemical (degradation by hydrolysis, tests of revelations) and spectral [UV-visible, 1H-NMR (1H and 13C, TOCSY-1D, DQF-COSY, NOESY-2D)] methods. These pigments were found to consist of cyanidin 3,5-O-β-D-diglu-copyranoside and pelargonidin 3。展开更多
A new C21 steroidal glycoside,neocynapanogenin F 3-O-β-D-thevetoside 1,as well as its known aglycone neocynapanogenin F 2,which have an aberrant 13,14:14,15-disecopregnane-type skeleton were isolated from the root of...A new C21 steroidal glycoside,neocynapanogenin F 3-O-β-D-thevetoside 1,as well as its known aglycone neocynapanogenin F 2,which have an aberrant 13,14:14,15-disecopregnane-type skeleton were isolated from the root of Cynanchum paniculatum.Their structures were elucidated on the basis of spectroscopic data analysis.These compounds showed certain cytotoxic activities in vitro.展开更多
In this study,analysis was performed of the macro characterization of solar cells with chlorophyll dye from Syzygium paniculatum,using scanning electron microscopy(SEM)and energy-dispersive X-ray(EDX)analysis.Solar ce...In this study,analysis was performed of the macro characterization of solar cells with chlorophyll dye from Syzygium paniculatum,using scanning electron microscopy(SEM)and energy-dispersive X-ray(EDX)analysis.Solar cells based on titanium dioxide(TiO_(2))nanomaterial and chlorophyll dye from S.paniculatum can increase efficiency due to flavonoids and batulinic acid content.Photoanode TiO_(2) is one of the essential factors determining the photovoltaic properties of dye-sensitized solar cells(DSSCs)and shade,which broadens the absorption spectrum.Furthermore,the method used in this research involved varying the colour of the S.paniculatum leaves,namely red(SP-Red),green(SP-Green)and a red-green mix(SP-Mix).From a macro analysis,SEM observations resulted in agglomerated and aggregated TiO_(2)-polyethylene glycol(PEG)-dye layers with irregular shapes.EDX observation resulted in a peak in Ti at 5 keV and all constituents were detected with an O:Ti ratio of 3.47:1 for FTO-TiO2/PEG using SP-Green.Measurement of voltage-current(IV)using a digital multimeter indicated that the best occurred in the DSSC with SP-Green,resulting in a short-circuit current density(Isc)of 0.0047 mA/cm2,an open-circuit voltage(Voc)of 0.432 V,a charging factor(FF)of 0.749 and an efficiency(η)of 3.724%.展开更多
基金Supported by Grant from Universitas Airlangga,Surabaya,Indonesia with Grant No.8714/UN3/KR/2013
文摘Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale.
文摘The structure of tubeimoside V (1), a new cyclic bisdesmoside, isolated from tubers of Bolbostemma paniculatum (Tu Bei Mu), was established by means of 2D NMR spectral and chemical methods. Compound 1 has inter-saccharide chain bridging by a dicrotalic acid to form a unique macrocyclic structure.
文摘Objective To study the triterpenoid saponin from bulbs of Bolbostemma paniculatum.Methods The compound was isolated by repeated silica gel chromatographies and its strcuture was elucidated on the basis of physico chemical property and spectral analysis.Results A novel triterpenoid saponin was isolated and determined as olean 12-en-28-oic acid,3-{[2-O-[6-O-[(3R)-4-carboxy-3-hydrox-y-3-methyl-1-oxobutyl]-β-D-glucopyranosyl]-β-D-glucopyranosyl]oxy}-2,16,23-trihydroxy-28-[2-O-α-L-rhamnose(1→2)-α-L-arabinopyranosyl]ester(Iφ.Conclusion Compound I is a novel compound named as dexylosyltubeimosideⅢ.
基金Science and Technology Project of Yunnan Provincial Department of Science and Technology(Grant No.2018FD081)Yunnan Local Colleges Applied Basic Research Projects[Grant Nos.2019FH001-(109),2017FH001-092,2017FH001-094 and 2018FH001-019]Youth Research Foundation of Qujing Normal University in 2019(Grant No.2019QN004)。
文摘Cynanchum paniculatum(Bunge) Kitagawa is usually used as an herbal medicine for treating many diseases. Paeonol is the main active component, and its content is the key indicator for quality control of C. paniculatum. In the present study, we developed a rapid, accurate and precise method for quantitation of paeonol in C. paniculatum using 1 H NMR spectra. The deuterated solvent of methanol-d4 enabled satisfactory separation of the signals to be integrated in 1 H NMR spectrum. H-6(δ 7.78) of 1 H NMR spectrum of C. paniculatum was selected as the feature signal for quantitation, and trimesic acid(TMA) was selected as an internal standard. Validation of the quantitative method was performed in terms of linearity, specificity, repeatability and stability. This is the first time to report quantitative 1 H NMR(qHNMR) applied to determine the content of paeonol in C. paniculatum and showed a wider linearity range than the reported quantitation of paeonol in others. The simple extraction of paeonol from C. paniculatum was rapid and will prompt the application of the developed method. This work implied that qHNMR represented a feasible alternative to HPLC-based methods for quantitation of paeonol in C. paniculatum, and it was suitable for the quality control of C. paniculatum.
基金supported financially by grants from the National Natural Science Foundation of China(Nos.31770389,81703393).
文摘Two new C21 steroidal glycosides,paniculatumosides H and I,together with four known ones were isolated from the roots of Cynanchum paniculatum(Bge.)Kitag.Their structures were identifed by spectroscopic methods including extensive 1D and 2D NMR techniques.All compounds were subjected to detect the anti-tobacco mosaic virus(TMV)activities and their cytotoxities against three human tumor cell lines(SMMC-7721,MDA-MB-231 and A549).The results showed that compounds 1 and 5 exhibited potent protective activities against TMV,while 2,4 and 6 had moderate efects on the SMMC7721 cancer cells viability.
文摘Two new sterols were isolated from bulbs of Bolbostemma paniculatum (Maxim.) Franquet. Their structures were elucidated as stigmasta-7, 22, 25-triene-3-O-nonadecanoic acid ester (1) and stigmasta-7, 22, 25-triene-3-O-B-D- (6'-palmitoyl) glucopyranoside (2) by chemical and spectroscopic methods.
文摘Background: A healthy normal skin is essential for a person’s physical and mental well being. It is an important aspect of their sexual attractiveness, a sense of well being and a sense of self confidence. Vitiligo is an acquired depigmentation disorder of skin affecting 1% - 4% of the world population. Neither life threatening nor symptomatic (except that depigmented patches burn easily when exposed to the sun) the effects of vitiligo can be cosmetically and psychologically devastating. Because the disease is still not understood, there is a plethora of different treatments approaches, but they are largely unsatisfactory from patient’s perspective. Objective: To report the outcomes from oral use of an infusion of leaves of Solanum paniculatum L., Jacaranda brasiliensis and Sonchus oleraceus for treatment of vitiligo. Ethical issues: Study approved by the Research Ethics Committee of Hospital Federal dos Servidores do Estado do Rio de Janeiro (CAAE: 32143314.9.0000.5252). All participants confirmed the condition of volunteer by signing the Informed Consent Form. Methods: From October 2014 to October 2015 twelve subjects consumed daily four glasses (800 ml) of proposed phytotherapeutic preparation. Results: All subjects were highly satisfied about their outcomes. Total depigmentation was considered as a positive outcome because it is cosmetically acceptable. Total depigmentation was observed in presence of 80% or more of depigmenteted patches. Conclusions: The phytotherapeutic preparation proposed is effective on treatment of depigmenteted patches in vitiligo.
文摘From Combretum paniculatum flowers, two diglucosylated derivatives from cyanidin and pelargonidin were identified using chromatographic (TLC), chemical (degradation by hydrolysis, tests of revelations) and spectral [UV-visible, 1H-NMR (1H and 13C, TOCSY-1D, DQF-COSY, NOESY-2D)] methods. These pigments were found to consist of cyanidin 3,5-O-β-D-diglu-copyranoside and pelargonidin 3。
基金supported by Fund for Distinguished Chinese Young Scholars of the National Natural Science Foundation of China(No.30325046).
文摘A new C21 steroidal glycoside,neocynapanogenin F 3-O-β-D-thevetoside 1,as well as its known aglycone neocynapanogenin F 2,which have an aberrant 13,14:14,15-disecopregnane-type skeleton were isolated from the root of Cynanchum paniculatum.Their structures were elucidated on the basis of spectroscopic data analysis.These compounds showed certain cytotoxic activities in vitro.
基金funded by the UPPM-Politeknik Negeri Bandung in the context of competitiveness between groups in the field of expertise.
文摘In this study,analysis was performed of the macro characterization of solar cells with chlorophyll dye from Syzygium paniculatum,using scanning electron microscopy(SEM)and energy-dispersive X-ray(EDX)analysis.Solar cells based on titanium dioxide(TiO_(2))nanomaterial and chlorophyll dye from S.paniculatum can increase efficiency due to flavonoids and batulinic acid content.Photoanode TiO_(2) is one of the essential factors determining the photovoltaic properties of dye-sensitized solar cells(DSSCs)and shade,which broadens the absorption spectrum.Furthermore,the method used in this research involved varying the colour of the S.paniculatum leaves,namely red(SP-Red),green(SP-Green)and a red-green mix(SP-Mix).From a macro analysis,SEM observations resulted in agglomerated and aggregated TiO_(2)-polyethylene glycol(PEG)-dye layers with irregular shapes.EDX observation resulted in a peak in Ti at 5 keV and all constituents were detected with an O:Ti ratio of 3.47:1 for FTO-TiO2/PEG using SP-Green.Measurement of voltage-current(IV)using a digital multimeter indicated that the best occurred in the DSSC with SP-Green,resulting in a short-circuit current density(Isc)of 0.0047 mA/cm2,an open-circuit voltage(Voc)of 0.432 V,a charging factor(FF)of 0.749 and an efficiency(η)of 3.724%.
