BACKGROUND： Studies have demonstrated that oxyhemoglobin （OxyHb） can induce brain cell apoptosis in vivo.OBJECTIVE： To observe the effects of exogenous nerve growth factor （NGF） on cerebral cortical neuronal Bcl...BACKGROUND： Studies have demonstrated that oxyhemoglobin （OxyHb） can induce brain cell apoptosis in vivo.OBJECTIVE： To observe the effects of exogenous nerve growth factor （NGF） on cerebral cortical neuronal Bcl-2 and Bax expression in mice with OxyHb-induced subarachnoid hemorrhage. DESIGN, TIME AND SETTING： A completely randomized grouping, controlled animal experiment was performed at the Experimental Center for Biomedicine, College of Medicine, Xi＇an Jiaotong University between February and April 2005. MATERIALS： Fifty-four healthy, male, adult, ICR mice were included in this study. Subarachnoid hemorrhage was induced by a subarachnoid injection of OxyHb in 48 mice. Mouse NGF was obtained from Xiamen Beidazhilu Bioengineering Co., Ltd., China.METHODS： All 54 mice were randomly divided into three groups： control （n = 6）, injury （n = 24）, and NGF （n = 24）. The NGF group received a subarachnoidal administration of OxyHb, immediately followed by a caudal vein injection of NGF （1 μg）. The injury group was injected with OxyHb, and subsequently with physiological saline. The control group only received intravenous physiological saline. MAIN OUTCOME MEASURES： At 1,6, 24, and 48 hours following subarachnoid hemorrhage induction, expression levels of Bcl-2 and Bax were detected by immunohistochemistry in the cerebral cortex 3 mm anterior and posterior to the injection site. RESULTS： At all time points following OxyHb injection, cerebral cortical Bax levels were significantly higher in the injured group than in the control and NGF groups （P 〈 0.01）. During the first 24 hours following OxyHb injection, cerebral cortical Bcl-2 levels were significantly lower in the injury group compared to the control group （P 〈 0.05 0.01）. Between 1 and 48 hours, Bcl-2 levels were significantly higher in the NGF group than in the injury group （P 〈 0.01 ）. CONCLUSION： Exogenous NGF can inhibit increased neuronal Bax expression and decreased Bcl-2 expression in the cerebral cortex of mice with OxyHb -induced subarachnoid hemorrhage.展开更多
We characterize the hemodynamic response changes near-infrared spectroscopy （NIRS） during the presentation of in the main olfactory bulb （MOB） of anesthetized rats with three different odorants： （i） plain air a...We characterize the hemodynamic response changes near-infrared spectroscopy （NIRS） during the presentation of in the main olfactory bulb （MOB） of anesthetized rats with three different odorants： （i） plain air as a reference （Blank）, （ii） 2-heptanone （HEP）, and （iii） isopropylbenzene （Ib）. Odorants generate different changes in the concentrations of oxy- hemoglobin. Our results suggest that NIRS technology might be useful in discriminating various odorants in a non-invasive manner using animals with a superb olfactory system.展开更多
2017年2月24日,国际学术期刊Nucleic Acids Research在线发表了中国科学院生物化学与细胞生物学研究所国家蛋白质科学中心（上海）丁建平研究组的最新研究成果：＂Structural and mechanistic insights into regulation of HBO1 histone...2017年2月24日,国际学术期刊Nucleic Acids Research在线发表了中国科学院生物化学与细胞生物学研究所国家蛋白质科学中心（上海）丁建平研究组的最新研究成果：＂Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2＂,展开更多
Aim To reseach the promotive role of passing intmportal veinous chemotherapy combined with hyperbaric oxygen after hepatocellular carcinoma resection.Methods Sixty-five HCC patients were classified into two groups wit...Aim To reseach the promotive role of passing intmportal veinous chemotherapy combined with hyperbaric oxygen after hepatocellular carcinoma resection.Methods Sixty-five HCC patients were classified into two groups with randomly. One group was performed byintraportal veinous chemotherpy, the other group was performed by intraportal veinous chemotherapy combined with hyperbaric oxygen. Among 65 HCC cases, 43 patientswere tested with hepatic function; 54 and 27 patients weretested in serum sIL-2R, T-SOD and Mn-SOD activityrespectively; Of the 48 patients were analysed relativefactors with recurrence, 23 HCC patients (HBO group) and25 patients (Non-HBO group) were given intraportal veinouschemotherapy for four courses with FMC scheme and werecompared.Results There were no obvious difference in serumbiochemical items sIL-2R, T-SOD and Mn-SOD activitybefore and after one week of the chemotherapy in the HBOgroup (P > 0.05 ). There were obvious differences ofbiochemical items (X^2= 9.01, P < 0.01 ), PT (X^2=4.22, P < 0.05 ) , sIL-2R (t= 5.74, P < 0.01), T-SOD (t=5.735, P <0.01 ) and Mn-SOD (t=8.66, P< 0.01) activity in serum between the HBO group and Non-HBO group after the chemotherapy. Although recurrcencerelating to the size of HCC (X^2= 8.18, P < 0.01 ),impairment of hepatic function (X^2= 4.70, P < 0.05) anddegree of hepatic cirrhosis (X^2= 3.95, P < 0.05) wasobvious, yet HBO group was higher than Non-HBO group inreducing recurrence (X^2= 5.30, P < 0.05).Conclusion hatraportal veinous regular chemotherapycombined with HBO can significantly reduce recurrrnce after HCC resection.展开更多
文摘BACKGROUND： Studies have demonstrated that oxyhemoglobin （OxyHb） can induce brain cell apoptosis in vivo.OBJECTIVE： To observe the effects of exogenous nerve growth factor （NGF） on cerebral cortical neuronal Bcl-2 and Bax expression in mice with OxyHb-induced subarachnoid hemorrhage. DESIGN, TIME AND SETTING： A completely randomized grouping, controlled animal experiment was performed at the Experimental Center for Biomedicine, College of Medicine, Xi＇an Jiaotong University between February and April 2005. MATERIALS： Fifty-four healthy, male, adult, ICR mice were included in this study. Subarachnoid hemorrhage was induced by a subarachnoid injection of OxyHb in 48 mice. Mouse NGF was obtained from Xiamen Beidazhilu Bioengineering Co., Ltd., China.METHODS： All 54 mice were randomly divided into three groups： control （n = 6）, injury （n = 24）, and NGF （n = 24）. The NGF group received a subarachnoidal administration of OxyHb, immediately followed by a caudal vein injection of NGF （1 μg）. The injury group was injected with OxyHb, and subsequently with physiological saline. The control group only received intravenous physiological saline. MAIN OUTCOME MEASURES： At 1,6, 24, and 48 hours following subarachnoid hemorrhage induction, expression levels of Bcl-2 and Bax were detected by immunohistochemistry in the cerebral cortex 3 mm anterior and posterior to the injection site. RESULTS： At all time points following OxyHb injection, cerebral cortical Bax levels were significantly higher in the injured group than in the control and NGF groups （P 〈 0.01）. During the first 24 hours following OxyHb injection, cerebral cortical Bcl-2 levels were significantly lower in the injury group compared to the control group （P 〈 0.05 0.01）. Between 1 and 48 hours, Bcl-2 levels were significantly higher in the NGF group than in the injury group （P 〈 0.01 ）. CONCLUSION： Exogenous NGF can inhibit increased neuronal Bax expression and decreased Bcl-2 expression in the cerebral cortex of mice with OxyHb -induced subarachnoid hemorrhage.
基金The MKE(The Ministry of Knowledge Economy),Korea,under the ITRC(Information Technology Research Center)support program supervised by the NIPA(National IT Industry Promotion Agency) (NIPA-2012-H0301-12-2006)Brain Research Center(BRC)(2012K001127),The MKE(10033634-2012-21)National Research Foundation of Korea(NRF)(2012-0005787)
文摘We characterize the hemodynamic response changes near-infrared spectroscopy （NIRS） during the presentation of in the main olfactory bulb （MOB） of anesthetized rats with three different odorants： （i） plain air as a reference （Blank）, （ii） 2-heptanone （HEP）, and （iii） isopropylbenzene （Ib）. Odorants generate different changes in the concentrations of oxy- hemoglobin. Our results suggest that NIRS technology might be useful in discriminating various odorants in a non-invasive manner using animals with a superb olfactory system.
文摘2017年2月24日,国际学术期刊Nucleic Acids Research在线发表了中国科学院生物化学与细胞生物学研究所国家蛋白质科学中心（上海）丁建平研究组的最新研究成果：＂Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2＂,
文摘Aim To reseach the promotive role of passing intmportal veinous chemotherapy combined with hyperbaric oxygen after hepatocellular carcinoma resection.Methods Sixty-five HCC patients were classified into two groups with randomly. One group was performed byintraportal veinous chemotherpy, the other group was performed by intraportal veinous chemotherapy combined with hyperbaric oxygen. Among 65 HCC cases, 43 patientswere tested with hepatic function; 54 and 27 patients weretested in serum sIL-2R, T-SOD and Mn-SOD activityrespectively; Of the 48 patients were analysed relativefactors with recurrence, 23 HCC patients (HBO group) and25 patients (Non-HBO group) were given intraportal veinouschemotherapy for four courses with FMC scheme and werecompared.Results There were no obvious difference in serumbiochemical items sIL-2R, T-SOD and Mn-SOD activitybefore and after one week of the chemotherapy in the HBOgroup (P > 0.05 ). There were obvious differences ofbiochemical items (X^2= 9.01, P < 0.01 ), PT (X^2=4.22, P < 0.05 ) , sIL-2R (t= 5.74, P < 0.01), T-SOD (t=5.735, P <0.01 ) and Mn-SOD (t=8.66, P< 0.01) activity in serum between the HBO group and Non-HBO group after the chemotherapy. Although recurrcencerelating to the size of HCC (X^2= 8.18, P < 0.01 ),impairment of hepatic function (X^2= 4.70, P < 0.05) anddegree of hepatic cirrhosis (X^2= 3.95, P < 0.05) wasobvious, yet HBO group was higher than Non-HBO group inreducing recurrence (X^2= 5.30, P < 0.05).Conclusion hatraportal veinous regular chemotherapycombined with HBO can significantly reduce recurrrnce after HCC resection.
