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BMS-345541 inhibited nuclear factor kappa B expression and improved locomotor function recovery in rats after acute spinal cord injury 被引量:1
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作者 Xin Han Shouyu Wang Zhen Zhang Decheng Lu Hairun Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第23期1775-1779,共5页
This study sought to elucidate the changes of nuclear factor kappa B (NF-KB) expression and locomotor function of hind limb after subdural injection of BMS-345541 was applied in rats with acute spinal cord injury. T... This study sought to elucidate the changes of nuclear factor kappa B (NF-KB) expression and locomotor function of hind limb after subdural injection of BMS-345541 was applied in rats with acute spinal cord injury. The results indicated that BMS-345541 treatment reduced the expression of NF-kB at 24 hours after injury, compared with normal saline-treated rats. This treatment also led to a significant improvement in locomotor functional recovery at 14 days after injury. Overall, the findings demonstrated that BMS-345541 significantly ameliorated spinal cord injury-induced hind limb dysfunction by inhibiting the expression of NF-kB after spinal cord injury. 展开更多
关键词 spinal cord injury BMS-345541 nuclear factor kappa B locomotor function neural regeneration
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Nuclear Effect on Gluon Distribution Function and Associate Production of J/Ψ and y with Large P_T in High Energy p-Fe Collision
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作者 Zhang Shu~2 He Zhe-min~(1,2) Peng Hong-an~(1,3) Duan Chun-gui~21 CCAST(World Laboratory)P.O.Box 8730,Biejing 1000802 Physics Department,Hebei Normal University,Shijiazhuang,0500163 Physics Department,Peking University,Beijing 100871 《Chinese journal of nuclear physics》 1995年第3期205-208,共4页
With three theoretical models of nuclear effects on gluon distribution functions,the differentialcross sections and the total cross sections for associate production of J/ψ and γ with large P_T in high energyp-Fe co... With three theoretical models of nuclear effects on gluon distribution functions,the differentialcross sections and the total cross sections for associate production of J/ψ and γ with large P_T in high energyp-Fe collisions are calculated. 展开更多
关键词 GLUON distribution function nuclear effect J/Ψ PRODUCTION
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Unit 1 of Ling’ao Nuclear Power Plant phase II underwent hot functional test successfully
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作者 Liu Chunsheng 《Electricity》 2010年第2期8-,共1页
On February 25, the Unit 1 of Ling’ao Nuclear Power Plant phase II underwent a 41-day-long hot functional test successfully with its major systems satisfying the requirements for
关键词 II TEST ao nuclear Power Plant phase II underwent hot functional test successfully Unit 1 of Ling
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前列腺素E1预处理抑制脊髓缺血再灌注损伤后模型大鼠内皮细胞的铁死亡
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作者 黄玉珊 王荣荣 +1 位作者 李湘淼 白金柱 《中国组织工程研究》 北大核心 2026年第22期5716-5727,共12页
背景:铁死亡是脊髓缺血再灌注损伤的重要病理机制。研究证实,前列腺素E1可减轻慢性脑灌注不足诱导的海马体中脑微血管内皮细胞损伤,但它对脊髓缺血再灌注损伤后内皮细胞铁死亡的作用尚缺乏深入研究。目的:探讨前列腺素E1预处理能否通过... 背景:铁死亡是脊髓缺血再灌注损伤的重要病理机制。研究证实,前列腺素E1可减轻慢性脑灌注不足诱导的海马体中脑微血管内皮细胞损伤,但它对脊髓缺血再灌注损伤后内皮细胞铁死亡的作用尚缺乏深入研究。目的:探讨前列腺素E1预处理能否通过抑制内皮细胞的铁死亡来减轻脊髓缺血再灌注损伤,并阐明可能的机制。方法:①细胞实验:将大鼠脊髓微血管内皮细胞分4组处理:对照组在常氧(体积分数20%O_(2))条件下加入完全培养基培养;模型组置于缺氧培养箱(体积分数95%N_(2)、5%CO_(2))中加入无葡萄糖无血清培养基3 h(氧-葡萄糖剥夺),随后在常氧(体积分数20%O_(2))条件下加入完全培养基培养12 h(复氧),建立氧-葡萄糖剥夺/复氧模型模拟脊髓缺血再灌注损伤;预处理组氧-葡萄糖剥夺3 h后继续培养2 h,添加前列腺素E1处理2 h后复氧培养12 h;抑制剂组氧-葡萄糖剥夺3 h后添加核因子红细胞2相关因子2抑制剂ML385处理2 h,再添加前列腺素E1处理2 h,复氧培养12 h。处理结束后,检测细胞内丙二醛、谷胱甘肽和Fe2+浓度,CCK-8法检测细胞活力,免疫荧光染色与Western blot检测酰基辅酶A合成酶长链家族成员4、谷胱甘肽过氧化物酶4表达,流式细胞术检测细胞内活性氧水平,Western blot检测核因子红细胞2相关因子2、血红素加氧酶1蛋白表达。②动物实验:将45只大鼠随机分为3组处理:假手术组(n=15)仅开腹,暴露腹主动脉但不阻断;模型组(n=15)夹闭腹主动脉30 min后经尾静脉注射生理盐水,随后恢复动脉血流(建立脊髓缺血再灌注损伤模型);预处理组(n=15)夹闭腹主动脉30 min后经尾静脉注射前列腺素E1,随后恢复动脉血流。恢复动脉血流24 h后,通过BBB评分、斜板实验和尼氏染色评估大鼠运动功能和神经元损伤情况,脊髓含水量检测、闭锁小带蛋白免疫荧光染色和CD34免疫组化染色评估血脊髓屏障完整性和微血管密度,免疫荧光染色、普鲁士蓝染色、Western blot和生化检测评估脊髓组织铁死亡情况。结果与结论:①细胞实验:氧-葡萄糖剥夺/复氧造模可降低大鼠脊髓微血管内皮细胞活力、诱导细胞铁死亡以及下调核因子红细胞2相关因子2、血红素加氧酶1蛋白表达,前列腺素E1预处理可抑制氧-葡萄糖剥夺/复氧造模对大鼠脊髓微血管内皮细胞的上述影响;ML385部分逆转了前列腺素E1预处理的作用。②动物实验:前列腺素E1预处理可减轻脊髓缺血再灌注损伤造成的大鼠运动功能缺陷与神经元损伤、血脊髓屏障损伤,改善微血管密度,抑制脊髓组织铁死亡。③结果表明,前列腺素E1预处理通过激活核因子红细胞2相关因子2/血红素加氧酶1信号通路抑制内皮细胞的铁死亡,对脊髓缺血再灌注损伤发挥保护作用。 展开更多
关键词 前列腺素E1 预处理 脊髓缺血再灌注损伤 铁死亡 内皮细胞 血脊髓屏障 神经功能 Nrf2/HO-1信号通路
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基于AEA-ITD3-MMC算法的核电蒸汽系统性能优化
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作者 董元发 张俊 +4 位作者 肖云龙 安友军 刘浩 张弦 郭鹏 《计算机集成制造系统》 北大核心 2026年第1期115-130,共16页
针对核电蒸汽系统的高维函数优化问题,传统进化算法存在精度差、收敛速度慢和极易陷入局部最优等问题。为此,通过结合传统进化算法和深度强化学习,提出了一种包含多机制协同和改进TD3(ITD3)的自适应进化算法(AEA-ITD3-MMC)。首先,引入... 针对核电蒸汽系统的高维函数优化问题,传统进化算法存在精度差、收敛速度慢和极易陷入局部最优等问题。为此,通过结合传统进化算法和深度强化学习,提出了一种包含多机制协同和改进TD3(ITD3)的自适应进化算法(AEA-ITD3-MMC)。首先,引入基于多机制协同的种群重构策略,以增强初始种群的质量;其次,采用平衡优选策略,增强算法的全局探索能力和局部开发能力;然后,通过对标准TD3算法进行改进,设计了针对单目标函数优化问题的状态空间、动作空间、决策变量更新策略和自适应终止条件等,极大地提升了标准TD3算法的局部搜索能力;最后,设计了子代种群的生成策略,以保持子代种群的收敛性和多样性。在数值实验中,首先利用CEC2014和CEC2017测试函数集对AEA-ITD3-MMC算法的改进算子进行有效性分析,论证了所有改进算子的有效性;然后通过与传统进化算法进行对比,证明了AEA-ITD3-MMC算法在整体性能上显著优于10种经典进化算法;最后将AEA-ITD3-MMC算法应用于某核电蒸汽系统的高维决策变量优化问题上,进一步论证了该算法在工程应用中的优越性。 展开更多
关键词 自适应进化算法 TD3算法 高维函数优化 核电蒸汽系统性能优化
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心俞穴位埋线预处理改善急性心肌缺血模型大鼠的心功能 被引量:1
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作者 陈小青 卞路瑶 +2 位作者 陆星宇 杨涛 李湘海 《中国组织工程研究》 北大核心 2026年第4期882-891,共10页
背景:针刺心俞穴能显著改善急性心肌缺血的心功能,保护心肌细胞,但心俞穴位埋线处理对急性心肌缺血心功能的效果及机制尚不明确。核因子κB激活常出现P65亚型的核内易位,核因子κB信号通路的活化标志是P65水平升高。目的:探究心俞穴位... 背景:针刺心俞穴能显著改善急性心肌缺血的心功能,保护心肌细胞,但心俞穴位埋线处理对急性心肌缺血心功能的效果及机制尚不明确。核因子κB激活常出现P65亚型的核内易位,核因子κB信号通路的活化标志是P65水平升高。目的:探究心俞穴位埋线预处理对急性心肌缺血大鼠心功能及白细胞介素10、肿瘤坏死因子α、P65基因和蛋白表达水平的影响。方法:采用随机数字表法将32只雄性SD大鼠分为空白组、模型组、心俞穴组、非经非穴组,每组8只,后3组构建急性心肌缺血大鼠模型。心俞穴组大鼠心俞穴位埋线14 d,随后背部皮下注射盐酸异丙肾上腺素构建急性心肌缺血大鼠模型;非经非穴组局部埋线14 d,余同上;模型组心俞仅标记,余同上;空白组心俞仅标记,随后背部皮下注射等量生理盐水。造模后24 h检测心电图、心脏超声,腹主动脉取血ELISA法检测血清肌酸激酶和肌酸激酶同工酶水平,随后麻醉处死大鼠取材。苏木精-伊红和TUNEL染色观察心肌组织病理学变化和心肌细胞凋亡情况;RT-qPCR和Western Blot法检测心肌组织肿瘤坏死因子α、白细胞介素10、P65 mRNA及蛋白表达。结果与结论:(1)心电图:与空白组比较,模型组、非经非穴组、心俞穴组心电图Ⅱ导联ST段显著抬高;(2)心脏超声:与模型组比较,心俞穴组左室收缩末期内径显著减小(P<0.05),左室射血分数、左室短轴短缩率显著升高(P<0.05);(3)血清肌酸激酶和肌酸激酶同工酶:与模型组比较,心俞穴组显著降低(P<0.05);(4)苏木精-伊红染色:与模型组比较,心俞穴组心肌纤维排列基本整齐,水肿较轻,有少许炎性细胞浸润;(5)TUNEL染色:与模型组比较,心俞穴组心肌细胞凋亡荧光强度显著降低,凋亡率显著降低(P<0.05);(6)RT-qPCR、WesternBlot:与模型组比较,心俞穴组心肌组织白细胞介素10表达水平显著增高(P<0.05)、肿瘤坏死因子α、P65表达水平显著降低(P<0.05);(7)提示心俞穴位埋线预处理可改善急性心肌缺血大鼠心功能,其作用机制可能与抑制核因子κB信号通路的活化有关。 展开更多
关键词 心俞 穴位埋线 急性心肌缺血 核因子κB 异丙肾上腺素 心功能 P65 白细胞介素10 工程化组织构建
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虾青素缓解新生大鼠坏死性小肠结肠炎发展的机制
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作者 赵乐 王晓林 《中国现代普通外科进展》 2026年第1期13-19,共7页
目的:探讨虾青素上调FGF21/PGC-1α通路改善新生大鼠坏死性小肠结肠炎(NEC)模型肠道黏膜线粒体功能和坏死性凋亡的机制。方法:妊娠第14天对大鼠进行剖宫产手术,取出早产SD大鼠,雌雄不限。早产大鼠分为3组:对照组、NEC模型组和虾青素组... 目的:探讨虾青素上调FGF21/PGC-1α通路改善新生大鼠坏死性小肠结肠炎(NEC)模型肠道黏膜线粒体功能和坏死性凋亡的机制。方法:妊娠第14天对大鼠进行剖宫产手术,取出早产SD大鼠,雌雄不限。早产大鼠分为3组:对照组、NEC模型组和虾青素组。通过qPCR检测各组大鼠FGF21/PGC-1α表达。通过HE染色分析大鼠小肠组织病理学。ELISA检测大鼠血清中白细胞介素-1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)含量及大鼠肠道组织中丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽(GSH)含量。通过TUNEL试剂盒检测细胞凋亡率。采用Western blot法检测炎症及凋亡相关蛋白半胱天冬酶-3(Caspase 3)、TNF-α、诱导型一氧化氮合酶(iNOS)、核因子-κB(NF-κB)、B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X(BAX)、Toll样受体4(TLR4)的表达。采用荧光素酶法和qPCR技术检测肠道组织三磷酸腺苷(ATP)和线粒体DNA(mtDNA)水平。通过JC-1双色荧光染色法检测线粒体膜电位(MMP)。通过qPCR检测线粒体相关基因核呼吸因子1(NRF1)和线粒体转录因子A(TFAM)的表达。通过吸光度变化分析NEC线粒体复合酶活性。结果:NEC模型组FGF21和PGC-1αmRNA表达较对照组降低(P<0.05),虾青素组较NEC模型组升高(P<0.05)。NEC模型组FGF21和PGC-1α蛋白表达较对照组降低,虾青素组较NEC模型组升高(P<0.05)。NEC模型组HE评分较对照组升高(P<0.05),虾青素组较NEC模型组降低(P<0.05),虾青素减轻NEC病理改变。NEC模型组IL-6、IL-1β和TNF-α水平较对照组升高,虾青素组较NEC模型组降低(P<0.05)。NEC模型组SOD和GSH水平较对照组降低,虾青素组较NEC模型组升高(P<0.05);NEC模型组MDA水平较对照组升高,虾青素组较NEC模型组降低(P<0.05),虾青素抑制NEC氧化应激升高。NEC模型组肠道组织细胞凋亡率较对照组升高,虾青素组细胞凋亡率较NEC模型组降低(P<0.05)。NEC模型组肠道组织Caspase 3表达较对照组升高,虾青素组较NEC模型组降低(P<0.05)。NEC模型组TNF-α、iNOS、NF-κB、BAX、TLR4表达高于对照组,虾青素组低于NEC模型(P<0.05);NEC模型组Bcl-2表达较对照组降低,虾青素组较NEC模型组升高(P<0.05)。NEC模型组ATP、mtDNA、MMP、NRF1和TFAM mRNA表达较对照组降低(P<0.05),虾青素组较NEC模型组升高(P<0.05)。NEC模型组复合物Ⅰ、复合物Ⅲ和复合物Ⅳ活性较对照组降低,虾青素组较NEC模型组升高(P<0.05)。结论:虾青素通过上调FGF21和PGC-1α通路,改善NEC模型肠道黏膜细胞线粒体功能,并减少细胞的坏死性凋亡。 展开更多
关键词 虾青素 FGF21/PGC-1α 坏死性小肠结肠炎 线粒体功能 核呼吸因子1 线粒体转录因子A 大鼠
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反应堆保护系统维护通信网络研究与设计
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作者 雷敏杰 汪凡雨 +4 位作者 陈起 赵洋 何晋宇 吴延群 汪亨 《自动化仪表》 2026年第1期20-24,共5页
当前,保护系统维护数据主要通过执行安全功能的安全级通信网络进行传输。但这不仅会大幅增加通信网络及主控制器运行负荷,还会限制维护数据及过程数据的传输。首先,对保护系统通信网络进行研究,总结了当前主流维护通信网络的优缺点。然... 当前,保护系统维护数据主要通过执行安全功能的安全级通信网络进行传输。但这不仅会大幅增加通信网络及主控制器运行负荷,还会限制维护数据及过程数据的传输。首先,对保护系统通信网络进行研究,总结了当前主流维护通信网络的优缺点。然后,提出了安全级设备中独立维护通信网络的设计方案。该方案基于功能安全要求,在维护通信协议中加入功能安全层,设计了完善的通信故障处理机制。基于独立性要求,完成了维护网络与安全级设备的通信隔离设计以及功能隔离设计。同时,依据相关标准对该方案进行了分析论证,确保独立的维护通信网络具有高可靠性且不会影响保护系统安全功能的执行。最后,通过搭建最小系统验证了该方案的可行性和有效性。该研究为后续保护系统维护通信的设计以及核电仪控系统的优化改进提供了参考。 展开更多
关键词 核电站 反应堆 保护系统 维护通信网络 功能安全 安全级通信
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Evaluation of Excitation Functions of Reactions Used in Production of Some Medical Radioisotopes
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作者 Idris Ahmad Yahya Ibrahim Yola Fatima Salman Koki 《International Journal of Medical Physics, Clinical Engineering and Radiation Oncology》 2017年第3期290-303,共14页
In this work, reaction cross-sections were calculated and Excitation Functions were evaluated for productions of 208Bi, 212,211,210At, 211,210Po isotopes using EXIFON code in the energy range from 0 MeV to 30 MeV. The... In this work, reaction cross-sections were calculated and Excitation Functions were evaluated for productions of 208Bi, 212,211,210At, 211,210Po isotopes using EXIFON code in the energy range from 0 MeV to 30 MeV. The code is based on an analytical model for statistical multistep direct and multistep compound reactions (SMD/SMC model). This work also investigates the shell structure effect on the reaction cross-section, the results obtained show that the cross-sections of (a, na) reaction for both with shell correction and without shell correction are zeros at energies range considered, this shows that the energy of the incident particle is below the threshold of this reaction due to the present of coulomb repulsive force between the projectile and target nucleus. 展开更多
关键词 nuclear Reaction CROSS-SECTION EXCITATION function RADIOISOTOPE nuclear Model
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Study of visualized simulation and analysis of nuclear fuel cycle system based on multilevel flow model 被引量:1
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作者 YOSHIKAWA Hidekazu 《Nuclear Science and Techniques》 SCIE CAS CSCD 2005年第6期358-370,共13页
Complex energy and environment system, especially nuclear fuel cycle system recently raised socialconcerns about the issues of economic competitiveness, environmental effect and nuclear proliferation. Only underthe co... Complex energy and environment system, especially nuclear fuel cycle system recently raised socialconcerns about the issues of economic competitiveness, environmental effect and nuclear proliferation. Only underthe condition that those conflicting issues are gotten a consensus between stakeholders with different knowledgebackground, can nuclear power industry be continuingly developed. In this paper, a new analysis platform has beendeveloped to help stakeholders to recognize and analyze various socio-technical issues in the nuclear fuel cycle systembased on the functional modeling method named Multilevel Flow Models (MFM) according to the cognition theoryof human being. Its character is that MFM models define a set of mass, energy and information flow structures onmultiple levels of abstraction to describe the functional structure of a process system and its graphical symbol representationand the means-end and part-whole hierarchical flow structure to make the represented process easy to beunderstood. Based upon this methodology, a micro-process and a macro-process of nuclear fuel cycle system wereselected to be simulated and some analysis processes such as economics analysis, environmental analysis and energybalance analysis related to those flows were also integrated to help stakeholders to understand the process of decision-making with the introduction of some new functions for the improved Multilevel Flow Models Studio, and finallythe simple simulation such as spent fuel management process simulation and money flow of nuclear fuel cycleand its levelised cost analysis will be represented as feasible examples. 展开更多
关键词 核燃料 功能模型 能源工业 核能 环境友好性 核电厂
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核化工项目安全级控制盘的设计研究
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作者 蔡孟杭 姜明月 +1 位作者 杨庆彧 刘郢 《自动化仪表》 2026年第1期43-48,共6页
安全级控制盘是核化工项目的关键设备。但随着工程实际应用,暴露出早期方案存在的扩展性、灵活性、小型化差等缺点。为此,在符合安全级控制系统设计原则的前提下,研究了更适用于核化工项目安全级控制盘的创新方案。基于工程应用反馈以... 安全级控制盘是核化工项目的关键设备。但随着工程实际应用,暴露出早期方案存在的扩展性、灵活性、小型化差等缺点。为此,在符合安全级控制系统设计原则的前提下,研究了更适用于核化工项目安全级控制盘的创新方案。基于工程应用反馈以及相似工程应用实例,结合安全及控制盘的功能与人因工程需求,针对安全级控制盘类型、盘面功能区域划分、盘面布置原则、报警和参数显示功能、设备控制和控制模式切换功能等进行设计优化。形成了一套满足当前核化工项目需求的安全级控制盘设计方案。该设计方案在国内实际工程中应用且效果良好,满足核化工项目的运维需求。该方案为我国后续同类工程的设计提供了技术积累与参考。 展开更多
关键词 核化工 安全级控制盘 控制系统 安全功能 人因工程
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Approximating the Radial Distribution Function of the Electron in a Hydrogen Atom by a Normal Distribution Suggests That Magnetic Confinement Fusion Would Be Less Energy Efficient than Inertial Confinement Fusion
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作者 Motohisa Osaka 《Applied Mathematics》 2024年第9期585-593,共9页
Since the position of the electron in a hydrogen atom cannot be determined, the region in which it resides is said to be determined stochastically and forms an electron cloud. The probability density function of the s... Since the position of the electron in a hydrogen atom cannot be determined, the region in which it resides is said to be determined stochastically and forms an electron cloud. The probability density function of the single electron in 1s orbit is expressed as φ2, a function of distance from the nucleus. However, the probability of existence of the electron is expressed as a radial distribution function at an arbitrary distance from the nucleus, so it is estimated as the probability of the entire spherical shape of that radius. In this study, it has been found that the electron existence probability approximates the radial distribution function by assuming that the probability of existence of the electron being in the vicinity of the nucleus follows a normal distribution for arbitrary x-, y-, and z-axis directions. This implies that the probability of existence of the electron, which has been known only from the distance information, would follow a normal distribution independently in the three directions. When the electrons’ motion is extremely restricted in a certain direction by the magnetic field of both tokamak and helical fusion reactors, the probability of existence of the electron increases with proximity to the nucleus, and as a result, it is less likely to be liberated from the nucleus. Therefore, more and more energy is required to free the nucleus from the electron in order to generate plasma. 展开更多
关键词 Electron Cloud Radial Distribution function nuclear Fusion TOKAMAK Laser
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Fermi Function and Its Applications 被引量:2
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作者 Asma A. Elahwel Najat A. ALjalali +1 位作者 Mariam Barbash Ali M. Awin 《Journal of Applied Mathematics and Physics》 2023年第1期135-146,共12页
In this paper, we give a definition of the Fermi function, or the so-called Woods-Saxon potential, a well-known potential in nuclear physics;then, we give a few of its applications as examples. Some important integral... In this paper, we give a definition of the Fermi function, or the so-called Woods-Saxon potential, a well-known potential in nuclear physics;then, we give a few of its applications as examples. Some important integrals, which involve this function, are computed discussing the integrability and convergence of these integrals. Following, we derive formulae that encounter the above-mentioned function to get nuclear and generalized moments;the radial Fourier transformation is also exposed. Some related applications are then given that use such important integrals;in particular, we give the computation in conjunction with the problem of getting the optical-model potential for heavy-ion interactions at intermediate energies. Finally, we conclude with important remarks to do with the evolution of the subject. 展开更多
关键词 Fermi function INTEGRALS Optical Model POTENTIAL nuclear Interactions CONVERGENCE
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丹皮酚调控NF-κB通路对系膜增生性肾小球肾炎大鼠肾组织炎症反应和氧化应激的作用机制
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作者 黄怡颖 邹忠丽 张健 《解剖学研究》 2026年第1期31-37,共7页
目的探讨丹皮酚能否调控NF-κB通路影响系膜增生性肾小球肾炎大鼠肾组织炎症反应和氧化应激。方法60大鼠随机分为假手术组(sham组)、系膜增生性肾小球肾炎模型大鼠组(MsPGN组)、系膜增生性肾小球肾炎模型大鼠给予丹皮酚干预组(PAE组)、... 目的探讨丹皮酚能否调控NF-κB通路影响系膜增生性肾小球肾炎大鼠肾组织炎症反应和氧化应激。方法60大鼠随机分为假手术组(sham组)、系膜增生性肾小球肾炎模型大鼠组(MsPGN组)、系膜增生性肾小球肾炎模型大鼠给予丹皮酚干预组(PAE组)、系膜增生性肾小球肾炎模型大鼠给予醋酸泼尼松干预阳性对照组(PAT组)和PAE组基础上给予NF-κB信号通路激活剂PMA干预组(PAE+PMA组),每组10只。检测尿液中24 h尿蛋白、尿素氮和血清中血肌酐含量;酶联免疫吸附试验(ELISA)检测肾组织中氧化应激和炎症水平;苏木素-伊红(HE)染色检测肾组织病理学变化;高碘酸-无色品红染色法(PAS)检测肾小球系膜细胞外基质(ECM)沉积;蛋白质印迹法检测肾组织中细胞外信号调节激酶(ERK)、磷酸化ERK(p-ERK)、核因子κB p65亚基(NF-κB p65)、磷酸化NF-κB(p-NF-κB p65)蛋白表达。结果与sham组相比,MsPGN组24 h尿蛋白、BUN、Scr含量、肾组织中TNF-α、IL-6、IL-1β水平、丙二醛(MDA)含量、系膜基质相对面积及肾组织中p-ERK/ERK比值、p-NF-κB p65/NF-κB p65比值均明显升高,肾组织中超氧化物歧化酶活性(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)含量降低(P<0.01);与MsPGN组相比,PAE组和PAT组24 h尿蛋白、血尿素氮(BUN)、血清肌酐(Scr)含量、肾组织中TNF-α、IL-6、IL-1β水平、MDA含量、系膜基质相对面积及肾组织中p-ERK/ERK比值、p-NF-κB p65/NF-κB p65比值均明显降低,肾组织中SOD活性、GSHPx含量升高(P<0.01);与PAE组相比,PAE+PMA组24 h尿蛋白、BUN、Scr含量、肾组织中TNF-α、IL-6、IL-1β水平、MDA含量、系膜基质相对面积及肾组织中p-ERK/ERK比值、p-NF-κB p65/NF-κB p65比值均明显升高,肾组织中SOD活性、GSH-Px含量降低(P<0.01)。结论丹皮酚可能通过抑制NF-κB途径抑制MsPGN大鼠氧化应激损伤,减轻炎症反应,从而改善MsPGN大鼠肾功能发挥改善作用。 展开更多
关键词 丹皮酚 系膜增生性肾小球肾炎 核因子-κB 氧化应激 炎症 肾功能
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7.0T nuclear magnetic resonance evaluation of the amyloid beta(1–40) animal model of Alzheimer's disease: comparison of cytology verification 被引量:6
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作者 Lei Zhang Shuai Dong +1 位作者 Guixiang Zhao Yu Ma 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第4期430-435,共6页
3.0T magnetic resonance spectroscopic imaging brain function in Alzheimer's disease. However, is a commonly used method in the research ot the role of 7.0T high-field magnetic resonance spectroscopic imaging in brain... 3.0T magnetic resonance spectroscopic imaging brain function in Alzheimer's disease. However, is a commonly used method in the research ot the role of 7.0T high-field magnetic resonance spectroscopic imaging in brain function of Alzheimer's disease remains unclear. In this study, 7.0T magnetic resonance spectroscopy showed that in the hippocampus of Alzheimer's disease rats, the N-acetylaspartate wave crest was reduced, and the creatine and choline wave crest was elevated. This finding was further supported by hematoxylin-eosin staining, which showed a loss of hippocampal neurons and more glial cells. Moreover, electron microscopy showed neuronal shrinkage and mitochondrial rupture, and scanning electron microscopy revealed small size hippocampal synaptic vesicles, incomplete synaptic structure, and reduced number. Overall, the results revealed that 7.0T high-field nuclear magnetic resonance spectroscopy detected the lesions and functional changes in hippocampal neurons of Alzheimer's disease rats in vivo, allowing the possibility for assessing the success rate and grading of the amyloid beta (1-40) animal model of Alzheimer's disease. 展开更多
关键词 nerve regeneration Alzheimer's disease AΒ1-40 high-field functional magnetic resonance nuclear magnetic resonance spectroscopy NEUROPATHOLOGY N-ACETYLASPARTATE CREATINE CHOLINE hippocampus NSFC grant neural regeneration
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维生素D_(3)辅助布地奈德吸入气雾剂对小儿支气管哮喘TLR4/NF-κB信号通路的影响
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作者 杜兰 《临床合理用药》 2026年第1期27-30,共4页
目的 分析维生素D_(3)辅助布地奈德吸入气雾剂对小儿支气管哮喘Toll样受体4(TLR4)/核转录因子-κB(NF-κB)信号通路的影响。方法 选取2022年5月—2024年12月黄骅市中医医院收治的支气管哮喘患儿86例开展研究,以密封信封抽签法随机分为... 目的 分析维生素D_(3)辅助布地奈德吸入气雾剂对小儿支气管哮喘Toll样受体4(TLR4)/核转录因子-κB(NF-κB)信号通路的影响。方法 选取2022年5月—2024年12月黄骅市中医医院收治的支气管哮喘患儿86例开展研究,以密封信封抽签法随机分为辅助组(n=43)和参考组(n=43)。参考组予以布地奈德吸入气雾剂治疗,辅助组在参考组基础上增加维生素D_(3)治疗,2组均持续给药14 d。比较2组疾病控制率,症状、体征改善时间,治疗前后TLR4、NF-κB蛋白表达水平、免疫指标。结果 辅助组疾病控制率为93.02%,高于参考组的76.74%(χ^(2)=4.440,P=0.035)。辅助组胸闷、憋喘、咳嗽、肺部湿啰音改善时间短于参考组(P<0.01)。治疗14 d后,2组TLR4、NF-κB蛋白表达水平低于治疗前,且辅助组低于参考组(P<0.01);2组CD_(3)^(+)、CD4^(+)、CD4^(+)/CD8^(+)高于治疗前,CD8^(+)低于治疗前,且辅助组高/低于参考组(P<0.05或P<0.01)。结论 维生素D_(3)辅助布地奈德吸入气雾剂可有效控制支气管哮喘患儿病情,促进症状体征改善,调节TLR4/NF-κB信号通路,提高免疫功能。 展开更多
关键词 支气管哮喘 小儿 维生素D_(3) 布地奈德吸入气雾剂 TOLL样受体4 核转录因子-ΚB 疾病控制率 免疫功能
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Calculations Energy of the (<i>nl</i><sup>2</sup>) <sup>1</sup><i>L<sup>π</sup></i>Doubly Excited States of Two-Electron Systems via the Screening Constant by Unit Nuclear Charge Formalism
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作者 Momar Talla Gning Ibrahima Sakho Malick Sow 《Journal of Modern Physics》 2020年第11期1891-1910,共20页
In this work, the total energies of doubly excited states (<em>ns</em><sup>2</sup>) <sup>1</sup>S<sup>e</sup>, (<em>np</em><sup>2</sup>) <sup&... In this work, the total energies of doubly excited states (<em>ns</em><sup>2</sup>) <sup>1</sup>S<sup>e</sup>, (<em>np</em><sup>2</sup>) <sup>1</sup>D<sup>e</sup>, (<em>nd</em><sup>2</sup>) <sup>1</sup>G<sup>e</sup>, (<em>nf</em><sup>2</sup>) <sup>1</sup>I<sup>e</sup>, (<em>ng</em><sup>2</sup>) <sup>1</sup>K<sup>e</sup>, and (<em>nh</em><sup>2</sup>) <sup>1</sup>M<sup>e</sup> of the helium isoelectronic sequence with Z ≤ 10 are calculated in the framework of the variational method of the Screening Constant by Unit Nuclear Charge (SCUNC). These calculations are performed using a new wavefunction correlated to Hylleraas-type. The possibility of using the SCUNC method in the investigation of high-lying Doubly Excited States(DES) in two-electron systems is demonstrated in the present work in the case of the (<em>nl</em><sup>2</sup>) <sup>1</sup><em>L</em><sup><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><em>&pi;</em></span></span></span></sup> doubly excited states, where accurate total energies are tabulated up to <em>n</em> = 20. All the results obtained in this paper are in agreement with the values of the available literature and may be useful for future experimental and theoretical studies on the doubly excited (<em>nl</em><sup>2</sup>) <sup>1</sup><em>L</em><sup><span style="white-space:nowrap;"><span style="white-space:nowrap;"><em>π</em></span></span></sup> states of two-electron systems. 展开更多
关键词 Doubly Excited States Helium-Like Systems Screening Constant by Unit nuclear Charge (SCUNC) Wave functions Correlated Total Energy
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Nrf2在2型糖尿病合并脑损伤后神经功能恢复中的作用研究
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作者 热依拉·牙合甫 杨烨 +1 位作者 李瑞晟 穆清爽 《脑与神经疾病杂志》 2025年第6期388-393,共6页
目的探讨核因子红细胞2相关因子2(Nrf2)在2型糖尿病合并脑损伤(T2D-BD)后神经功能恢复中的作用及相关机制.方法使用C57BL/6小鼠建立T2D模型和T2D-BD模型,并进行神经功能评分.采用Western blot检测小鼠脑组织中Nrf2和γH2AX蛋白表达水平... 目的探讨核因子红细胞2相关因子2(Nrf2)在2型糖尿病合并脑损伤(T2D-BD)后神经功能恢复中的作用及相关机制.方法使用C57BL/6小鼠建立T2D模型和T2D-BD模型,并进行神经功能评分.采用Western blot检测小鼠脑组织中Nrf2和γH2AX蛋白表达水平.构建Nrf2敲除(Nrf2^(-/-))小鼠模型,对Nrf2^(+/+)和Nrf2^(-/-)C57BL/6小鼠进行T2D-BD建模,并进行神经功能评分.检测脑组织中的γH2AX蛋白表达、总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)以及白细胞介素-6(IL-6)和肿瘤坏死因子α(TNF-α)水平.结果与T2D组相比,T2D-BD组小鼠神经功能评分显著升高,脑组织中Nrf2和γH2AX蛋白表达水平增加,T-SOD、GSH及IL-6、TNF-α水平升高,而MDA水平降低(P<0.05).与Nrf2^(+/+)组相比,Nrf2^(-/-)组小鼠神经功能评分升高,脑组织中γH2AX蛋白表达水平增加,T-SOD、GSH及IL-6、TNF-α水平升高,而MDA水平降低(P<0.05).结论Nrf2能够缓解小鼠T2D-BD的症状,减少脑组织氧化应激反应和炎症水平,并促进神经功能的恢复. 展开更多
关键词 2型糖尿病 脑损伤 神经功能 核因子红细胞2相关因子2
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苦黄注射液联合熊去氧胆酸治疗胆汁淤积型肝炎的效果评价及对Nrf2/HO-1信号通路因子的影响
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作者 王亚钦 李旭飞 孙趁意 《海南医学》 2025年第24期3522-3528,共7页
目的探讨苦黄注射液联合熊去氧胆酸治疗胆汁淤积型肝炎的效果评价及对核因子E2相关因子2(Nrf2)/血红素加氧酶-1(HO-1)信号通路因子的影响。方法选取郑州市第三人民医院2022年1月至2024年12月胆汁淤积型肝炎患者102例作为观察对象进行前... 目的探讨苦黄注射液联合熊去氧胆酸治疗胆汁淤积型肝炎的效果评价及对核因子E2相关因子2(Nrf2)/血红素加氧酶-1(HO-1)信号通路因子的影响。方法选取郑州市第三人民医院2022年1月至2024年12月胆汁淤积型肝炎患者102例作为观察对象进行前瞻性研究,按照随机数表法分为常规组51例、观察组51例。两组患者均给予基础治疗,同时常规组患者给予熊去氧胆酸,观察组患者予以熊去氧胆酸联合苦黄注射液,连续治疗两周。比较两组患者的治疗效果,治疗前及治疗两周后肝功能指标[总胆汁酸(TBA)、总胆红素(TBIL)、天冬氨酸氨基转移酶(AST)、丙氨酸转氨酶(ALT)、γ-谷氨酰基转移酶(GGT)]、肝纤维化指标[Ⅲ型前胶原(PC-Ⅲ)、透明质酸(HA)、层黏连蛋白(LN)、Ⅳ型胶原蛋白(Ⅳ-C)]、Nrf2/HO-1信号通路因子[Nrf2、HO-1、醌氧化还原酶-1(NQO1)]、炎性因子[白细胞介素-1β(IL-1β)、转化生长因子β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)]水平,统计不良反应发生率。结果观察组患者的总有效率为94.12%,明显高于常规组的80.39%,差异有统计学意义(P<0.05);治疗两周后观察组患者的血清TBIL、TBA、ALT、AST、GGT水平分别为(18.74±4.82)μmol/L、(16.57±4.05)μmol/L、(45.13±7.02)U/L、(43.20±8.89)U/L、(105.32±13.35)U/L,明显低于常规组(25.40±5.27)μmol/L、(23.92±5.10)μmol/L、(51.77±8.46)U/L、(51.18±10.24)U/L、(121.47±17.82)U/L,差异均有统计学意义(P<0.05);治疗两周后观察组患者的血清LN、HA、PC-Ⅲ、Ⅳ-C水平分别为(108.51±14.29)ng/L、(203.10±30.47)ng/L、(160.22±21.08)ng/L、(113.30±15.52)ng/L,明显低于常规组的(126.34±18.02)ng/L、(231.68±34.28)ng/L、(181.40±24.64)ng/L、(132.43±17.14)ng/L,差异均有统计学意义(P<0.05);治疗两周后观察组患者的血清Nrf2、HO-1、NQO1分别为(3.15±0.41)ng/mL、(2.33±0.40)ng/mL、(2.88±0.53)ng/mL,明显高于常规组(2.80±0.38)ng/mL、(1.98±0.34)ng/mL、(2.41±0.44)ng/mL,差异均有统计学意义(P<0.05);治疗两周后观察组患者的血清IL-1β、TNF-α、TGF-β1水平分别为(15.32±3.21)pg/mL、(43.16±8.73)pg/mL、(30.26±4.38)ng/mL,明显低于常规组(18.69±4.06)pg/mL、(60.10±12.27)pg/mL、(38.15±6.75)ng/mL,差异均有统计学意义(P<0.05);观察组患者的不良反应发生率11.76%,与常规组的7.84%比较差异无统计学意义(P>0.05)。结论苦黄注射液联合熊去氧胆酸治疗胆汁淤积型肝炎疗效确切,可改善肝功能,延缓肝纤维化进程,可能与其调节Nrf2/HO-1信号通路因子、炎症因子水平有关。 展开更多
关键词 胆汁淤积型肝炎 苦黄注射液 熊去氧胆酸 肝功能 核因子E2相关因子2/血红素加氧酶-1信号通路
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Study of A Multi-criteria Evaluation Methodology for Nuclear Fuel Cycle System Based on Sustainability
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作者 Hidekazu Yoshikawa 《工程科学(英文版)》 2006年第1期33-39,共7页
This paper presents a multi-criteria evaluation methodology for nuclear fuel cycle options in terms of energy sustainability. Starting from the general sustainability concept and the public acceptance questionnaire, a... This paper presents a multi-criteria evaluation methodology for nuclear fuel cycle options in terms of energy sustainability. Starting from the general sustainability concept and the public acceptance questionnaire, a set of indicators reflecting specific criteria for the evaluation of nuclear fuel cycle options are defined. Particular attention is devoted to the resource utility efficiency, environmental effect, human health hazard and economic effect, which represent the different concerns of different stakeholders. This methodology also integrated a special mathematic processing approach, namely the Extentics Evaluation Method, which quantifies the human being subjective perception to provide the intuitionistic judgement and comparison for different options. The once-through option and reprocessing option of nuclear fuel cycle are examined by using the proposed methodology. The assessment process and result can give us some guidance in nuclear fuel cycle evaluation under the constraint of limited data. 展开更多
关键词 核燃料循环系统 多标准评估方法论 主观感知 核能
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