An increased amount of DNA fragme ntation in the spermatozoa(SDF)is linked to male in fertility.The Sperm Chromati n Structure Assay(SCSA)is widely used for analysis of SDF.However,the current software(SCSASoftR)linke...An increased amount of DNA fragme ntation in the spermatozoa(SDF)is linked to male in fertility.The Sperm Chromati n Structure Assay(SCSA)is widely used for analysis of SDF.However,the current software(SCSASoftR)linked to this assay is licensed and often located within larger diagnostic centers.In this study,we present a protocol for using other types of software than SCSASoftR to determine the SDF index(DFI)with clinical relevance.This protocol is engineered after collect!ng and analyzing 254 samples from fertility patients and sperm donors over a 15-month period.DFI is analyzed using a strict protocol where the spermatozoa are treated with a strong acid(pH 1.2)followed by acridine orange.DFI is determined by a standard flow cytometric software,FACSDiva 6.1.3.Analysis of the outcome of the fertility treatment is included for 137 patients receiving either intrauterine inseminations(IUI)or timed coitus(TC).The results show that the chance of pregnancy decli nes as DFI in creases.We also found that the male DFI affects the chanee of pregnancy independent of the female age.We have shown that a standard flow cytometric software can be used when determi ning a clinical releva nt DFI.These findings are a sign ificant step toward impleme nting the an alysis as a part of the routi ne,in・house diag no sing of the male fertility patient and subseque ntly optimizing the treatme nt course of the couple with reduced human and financial costs.展开更多
The stray crystals seriously affect the properties of metals which fabricated by directional solidification(DS). In order to observe the formation of the stray crystals, the growth behavior of Sn-10 wt.%Bi alloy was o...The stray crystals seriously affect the properties of metals which fabricated by directional solidification(DS). In order to observe the formation of the stray crystals, the growth behavior of Sn-10 wt.%Bi alloy was observed by synchrotron X-ray imaging technique using self-developed directional solidification(DS) apparatus. The experimental results show that Sn–10 wt.%Bi alloy is oriented with the form of dendrites to grow into the liquid phase during the DS process, and dendrites are arranged neatly to form columnar crystal microstructures. It is also found that parts of the dendrite arms begin to crack. These cracked dendrites drift upward to grow up in front of the solid-liquid interface and form stray crystals.展开更多
文摘An increased amount of DNA fragme ntation in the spermatozoa(SDF)is linked to male in fertility.The Sperm Chromati n Structure Assay(SCSA)is widely used for analysis of SDF.However,the current software(SCSASoftR)linked to this assay is licensed and often located within larger diagnostic centers.In this study,we present a protocol for using other types of software than SCSASoftR to determine the SDF index(DFI)with clinical relevance.This protocol is engineered after collect!ng and analyzing 254 samples from fertility patients and sperm donors over a 15-month period.DFI is analyzed using a strict protocol where the spermatozoa are treated with a strong acid(pH 1.2)followed by acridine orange.DFI is determined by a standard flow cytometric software,FACSDiva 6.1.3.Analysis of the outcome of the fertility treatment is included for 137 patients receiving either intrauterine inseminations(IUI)or timed coitus(TC).The results show that the chance of pregnancy decli nes as DFI in creases.We also found that the male DFI affects the chanee of pregnancy independent of the female age.We have shown that a standard flow cytometric software can be used when determi ning a clinical releva nt DFI.These findings are a sign ificant step toward impleme nting the an alysis as a part of the routi ne,in・house diag no sing of the male fertility patient and subseque ntly optimizing the treatme nt course of the couple with reduced human and financial costs.
基金financially supported by the project of Yangtze Normal University under grant No.2017KYQD130 and No.2017XJQN08
文摘The stray crystals seriously affect the properties of metals which fabricated by directional solidification(DS). In order to observe the formation of the stray crystals, the growth behavior of Sn-10 wt.%Bi alloy was observed by synchrotron X-ray imaging technique using self-developed directional solidification(DS) apparatus. The experimental results show that Sn–10 wt.%Bi alloy is oriented with the form of dendrites to grow into the liquid phase during the DS process, and dendrites are arranged neatly to form columnar crystal microstructures. It is also found that parts of the dendrite arms begin to crack. These cracked dendrites drift upward to grow up in front of the solid-liquid interface and form stray crystals.
