Camellia nitidissima is a species of shrub or small tree whose leaves and leaf buds are used to produce tea. In recent years, C. nitidissima planting area has been increasing, resulting in the growing gap between the ...Camellia nitidissima is a species of shrub or small tree whose leaves and leaf buds are used to produce tea. In recent years, C. nitidissima planting area has been increasing, resulting in the growing gap between the supply and demand of C. nitidissima seedlings. At present, C. nitidissima can be propagated through seeding, cutting, grafting, plant tissue culture and air layering. Among them, cutting and tissue culture are the most commonly used methods for rapid propagation of C. nitidissima. Optimal rooting medium, type and concentration of hormones and environmental conditions are critical factors for C. nitidissima propagation by cuttings, while detoxification and optimization of medium composition are important for C. nitidissima propagation by tissue culture. This paper reviews recent pro- gress in propagation techniques for C. nitidissima, and finally puts forward some prospects and suggestions in C. nitidissima propagation.展开更多
[Objectives]This study was conducted to explore active components and molecular biological mechanisms of Camellia nitidissima Chi in the treatment of premature ovarian failure(POF).[Methods]The active components and c...[Objectives]This study was conducted to explore active components and molecular biological mechanisms of Camellia nitidissima Chi in the treatment of premature ovarian failure(POF).[Methods]The active components and corresponding targets of C.nitidissima Chi were retrieved through literature and the TCMSP database.POF-related disease targets were identified using the OMIM and Genecards databases.A PPI network was constructed using the STRING database and Cytoscape 3.10.2.A herb-active component-target-pathway network diagram was also constructed with Cytoscape 3.10.2.The CytoNCA plugin was used to screen out the top five core targets and core active components.GO and KEGG enrichment analyses of the intersecting targets were performed using DAVID.Finally,molecular docking was conducted using Auto Dock to verify the interaction between core targets and active components,and visualization was done using PyMol.[Results]A total of 26 active components and 461 targets of C.nitidissima Chi were identified,with 154 intersecting targets related to POF.The core components of the herb included 1,1'-bi-2-naphthol,3',4-O-dimethylcedrusin,eriodictyol,quercetin,and vanillin.The PPI network revealed that the main targets were epidermal growth factor receptor(EGFR),protein kinase B1(AKT1),proto-oncogene Src(SRC),hypoxia-inducible factor 1α(HIF1A),and estrogen receptor 1(ESR1).KEGG enrichment analysis revealed 10 pathways closely related to POF,mainly involving the PI3K-Akt signaling pathway,chemical carcinogenesis-reactive oxygen species,endocrine resistance,and the HIF-1 signaling pathway.Molecular docking results showed that the core active components had strong binding activity with the targets.[Conclusions]C.nitidissima Chi has multi-component,multi-target,and multi-pathway characteristics in the comprehensive treatment of POF,providing informational support for its clinical application.展开更多
Objective:Camellia nitidissima Chi,a Chinese medicine commonly used by ethnic minorities in Guangxi,China,is beneficial for clearing heat,detoxifying,inducing diuresis,and suppressing swelling.It has various pharmacol...Objective:Camellia nitidissima Chi,a Chinese medicine commonly used by ethnic minorities in Guangxi,China,is beneficial for clearing heat,detoxifying,inducing diuresis,and suppressing swelling.It has various pharmacological properties,including antitumor,anti-inflammatory,and antioxidant.However,its potential application in radioprotection remains unclear.In this study,we aimed to determine whether Camellia nitidissima Chi has radioprotective effects against radiation-induced gastrointestinal and hematopoietic damage.Methods:The 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)techniques were used to assess the ability of Camellia nitidissima Chi to scavenge free radicals.We conducted a 30-day survival rate experiment to evaluate the radioprotective capabilities of Camellia nitidissima Chi.Additionally,we developed models of radiation-induced intestinal and hematopoietic damage.Alterations in the white blood cell(WBC)count,total superoxide dismutase(T-SOD),glutathione(GSH),and protein expression linked to apoptosis were observed.Results:Camellia nitidissima Chi scavenged 84.72%and 93.47%of DPPH and ABTS,had a certain radiation protection potential,and increased the survival rate of mice to over 90%.Moreover,following exposure,Camellia nitidissima Chi enhanced WBC,T-SOD,and GSH levels.Camellia nitidissima Chi increased B-cell lymphoma-extra large(BCL-XL)expression and suppressed Bcl-2 associated X protein(BAX)expression,providing radioprotection to cells.Conclusions:Camellia nitidissima Chi has a strong antioxidant ability;it can improve the survival rate of mice after lethal dose irradiation and protect against radiation-induced hematopoietic and gastrointestinal damage.These findings can serve as a guide for using Chinese medicines for radioprotection.展开更多
Objective To isolate and identify the bioactive phytochemicals from the leaves of Camellia nitidissima. Methods The chemical constituents were isolated and purified by repeated silica gel, Sephadex LH-20, MCI gel colu...Objective To isolate and identify the bioactive phytochemicals from the leaves of Camellia nitidissima. Methods The chemical constituents were isolated and purified by repeated silica gel, Sephadex LH-20, MCI gel columns, recrystallization, and semi-preparative HPLC techniques. The chemicl structures of these compounds were identified on the basis of spectral data including NMR and MS. Then quorum sensing inhibition （QSI） activities of these compounds were tested using Chromobacterium violaceum CV026 as the bioindicator strain. The antitumor activities of these compounds were measured using SGC7901 as cell proliferation and cytotoxicity. Results cx-Spinasteryl-I^-D-glucopyranoside （1）, stigmasta-7,22-diene-3-O-[c^-L-arabinopyranosyl （1 -2）]-β-D-galactopyranoside （2）, kaempferol 3-O-[2-O-（trans-p-coumaroyl）-3-O-α -D-glucopyranosyl]-α-D-glucopyranoside （3）, aromadendrin （4）, catechin （5）, phlorizin 4＇-O-β-D-glucopyranoside （6）, （3R,6R,7Lg-3-hydroxy-4,7-megastigmadien- 9-one （7）, dodecanoic acid （8）, 3α-acetoxy-20-1upanol （9）, and 3β,6α- trihydroxyolean- 7-one （1 0） were successively isolated from the leaves of C. nitidissima. Unfortunately, these compounds had no QSI activity. Based on Cell Counting Kit-8 （CCK-8） assay, compound 10 showed the best anti-tumor activity or all compounds （ICs0 = 91.7 μg/mL）. Conclusion Apart from compounds 4 and 5, other eight compounds are reported in this plant for the first time. All compounds show no QSI activity, compound 10 shows potential cytotoxic activity on SGC7901 cells in vitro.展开更多
为改善巴沙鱼糜品质,将金花茶多糖(Camellia nitidissima Chi polysaccharide,CNCP)加入巴沙鱼糜,通过两段加热法制成CNCP-巴沙鱼糜复合凝胶。考察不同CNCP添加量(0%、0.2%、0.4%、0.6%、0.8%、1.0%)对CNCP-巴沙鱼糜复合凝胶的白度值、...为改善巴沙鱼糜品质,将金花茶多糖(Camellia nitidissima Chi polysaccharide,CNCP)加入巴沙鱼糜,通过两段加热法制成CNCP-巴沙鱼糜复合凝胶。考察不同CNCP添加量(0%、0.2%、0.4%、0.6%、0.8%、1.0%)对CNCP-巴沙鱼糜复合凝胶的白度值、凝胶强度、质构特性、感官评价、蒸煮损失率、持水性、水分分布、动态流变学特性、凝胶形成作用力、蛋白质二级结构、微观结构的影响。结果表明:随着CNCP添加量的增多,凝胶性能呈先升后降的趋势,并在添加量为0.4%时达到峰值。此时,鱼糜凝胶强度为202.59 g·cm,硬度、感官评分、持水性相比对照组分别提升20.1%、35.02%、23.2%,具有最高的储能模量(G′),自由水含量仅为2.3%,不易流动水的弛豫时间显著缩短,蒸煮损失率显著降低,二硫键含量显著增加(P<0.05),α-螺旋向更稳定的β-折叠结构转变,此时微观结构孔隙最小,分形维数最大,凝胶网状结构最为致密。综上所述,CNCP的添加有助于与巴沙鱼糜形成致密的复合凝胶网络,从而改善鱼糜凝胶品质,为进一步开发优质健康的鱼糜产品提供新的思路。展开更多
基金Supported by Guangxi Agricultural Comprehensive Development and Utilization Project from the International Fund for Agricultural Development(L-I-CN855)Science and Technology Development Project of Nanning City(20162090)+1 种基金Science and Technology Development Project of Fangchenggang City(Fang Ke AB17053004)the Fund of Guangxi Academy of Agricultural Science(Gui Nong ke 2015YZ20,Gui Nong Ke 2015YT89)
文摘Camellia nitidissima is a species of shrub or small tree whose leaves and leaf buds are used to produce tea. In recent years, C. nitidissima planting area has been increasing, resulting in the growing gap between the supply and demand of C. nitidissima seedlings. At present, C. nitidissima can be propagated through seeding, cutting, grafting, plant tissue culture and air layering. Among them, cutting and tissue culture are the most commonly used methods for rapid propagation of C. nitidissima. Optimal rooting medium, type and concentration of hormones and environmental conditions are critical factors for C. nitidissima propagation by cuttings, while detoxification and optimization of medium composition are important for C. nitidissima propagation by tissue culture. This paper reviews recent pro- gress in propagation techniques for C. nitidissima, and finally puts forward some prospects and suggestions in C. nitidissima propagation.
基金Supported by Guangxi Natural Science Foundation(2020GXNSFAA238022)National Natural Sciences Foundation of China(82060280)Science and Technology Plan Project of Guangxi University of Chinese Medicine(2018ZD003).
文摘[Objectives]This study was conducted to explore active components and molecular biological mechanisms of Camellia nitidissima Chi in the treatment of premature ovarian failure(POF).[Methods]The active components and corresponding targets of C.nitidissima Chi were retrieved through literature and the TCMSP database.POF-related disease targets were identified using the OMIM and Genecards databases.A PPI network was constructed using the STRING database and Cytoscape 3.10.2.A herb-active component-target-pathway network diagram was also constructed with Cytoscape 3.10.2.The CytoNCA plugin was used to screen out the top five core targets and core active components.GO and KEGG enrichment analyses of the intersecting targets were performed using DAVID.Finally,molecular docking was conducted using Auto Dock to verify the interaction between core targets and active components,and visualization was done using PyMol.[Results]A total of 26 active components and 461 targets of C.nitidissima Chi were identified,with 154 intersecting targets related to POF.The core components of the herb included 1,1'-bi-2-naphthol,3',4-O-dimethylcedrusin,eriodictyol,quercetin,and vanillin.The PPI network revealed that the main targets were epidermal growth factor receptor(EGFR),protein kinase B1(AKT1),proto-oncogene Src(SRC),hypoxia-inducible factor 1α(HIF1A),and estrogen receptor 1(ESR1).KEGG enrichment analysis revealed 10 pathways closely related to POF,mainly involving the PI3K-Akt signaling pathway,chemical carcinogenesis-reactive oxygen species,endocrine resistance,and the HIF-1 signaling pathway.Molecular docking results showed that the core active components had strong binding activity with the targets.[Conclusions]C.nitidissima Chi has multi-component,multi-target,and multi-pathway characteristics in the comprehensive treatment of POF,providing informational support for its clinical application.
基金sponsored by the National Natural Science Foundation of China(82303681 and 82202950)Fundamental Research Funds for the Central Universities(3332022063)+6 种基金the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2021-I2M-1-042)The National Natural Science Foundation of China(82202950)the Fundamental Research Funds for the Central Universities(3332022063)Feifei Xu,who participated in the research design.The National Natural Science Foundation of China(82303681)Wenfeng Gou,who participated in the research designThe Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2021-I2M-1-042)Wenbin Hou,who designed and conceived the study.
文摘Objective:Camellia nitidissima Chi,a Chinese medicine commonly used by ethnic minorities in Guangxi,China,is beneficial for clearing heat,detoxifying,inducing diuresis,and suppressing swelling.It has various pharmacological properties,including antitumor,anti-inflammatory,and antioxidant.However,its potential application in radioprotection remains unclear.In this study,we aimed to determine whether Camellia nitidissima Chi has radioprotective effects against radiation-induced gastrointestinal and hematopoietic damage.Methods:The 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)techniques were used to assess the ability of Camellia nitidissima Chi to scavenge free radicals.We conducted a 30-day survival rate experiment to evaluate the radioprotective capabilities of Camellia nitidissima Chi.Additionally,we developed models of radiation-induced intestinal and hematopoietic damage.Alterations in the white blood cell(WBC)count,total superoxide dismutase(T-SOD),glutathione(GSH),and protein expression linked to apoptosis were observed.Results:Camellia nitidissima Chi scavenged 84.72%and 93.47%of DPPH and ABTS,had a certain radiation protection potential,and increased the survival rate of mice to over 90%.Moreover,following exposure,Camellia nitidissima Chi enhanced WBC,T-SOD,and GSH levels.Camellia nitidissima Chi increased B-cell lymphoma-extra large(BCL-XL)expression and suppressed Bcl-2 associated X protein(BAX)expression,providing radioprotection to cells.Conclusions:Camellia nitidissima Chi has a strong antioxidant ability;it can improve the survival rate of mice after lethal dose irradiation and protect against radiation-induced hematopoietic and gastrointestinal damage.These findings can serve as a guide for using Chinese medicines for radioprotection.
基金National High Technology Research and Development Program of China(863 Program)(2014AA022208)National Natural Science Foundation of China(31170131 and 31070312)Jiangsu Qinglan Project
文摘Objective To isolate and identify the bioactive phytochemicals from the leaves of Camellia nitidissima. Methods The chemical constituents were isolated and purified by repeated silica gel, Sephadex LH-20, MCI gel columns, recrystallization, and semi-preparative HPLC techniques. The chemicl structures of these compounds were identified on the basis of spectral data including NMR and MS. Then quorum sensing inhibition （QSI） activities of these compounds were tested using Chromobacterium violaceum CV026 as the bioindicator strain. The antitumor activities of these compounds were measured using SGC7901 as cell proliferation and cytotoxicity. Results cx-Spinasteryl-I^-D-glucopyranoside （1）, stigmasta-7,22-diene-3-O-[c^-L-arabinopyranosyl （1 -2）]-β-D-galactopyranoside （2）, kaempferol 3-O-[2-O-（trans-p-coumaroyl）-3-O-α -D-glucopyranosyl]-α-D-glucopyranoside （3）, aromadendrin （4）, catechin （5）, phlorizin 4＇-O-β-D-glucopyranoside （6）, （3R,6R,7Lg-3-hydroxy-4,7-megastigmadien- 9-one （7）, dodecanoic acid （8）, 3α-acetoxy-20-1upanol （9）, and 3β,6α- trihydroxyolean- 7-one （1 0） were successively isolated from the leaves of C. nitidissima. Unfortunately, these compounds had no QSI activity. Based on Cell Counting Kit-8 （CCK-8） assay, compound 10 showed the best anti-tumor activity or all compounds （ICs0 = 91.7 μg/mL）. Conclusion Apart from compounds 4 and 5, other eight compounds are reported in this plant for the first time. All compounds show no QSI activity, compound 10 shows potential cytotoxic activity on SGC7901 cells in vitro.
文摘为改善巴沙鱼糜品质,将金花茶多糖(Camellia nitidissima Chi polysaccharide,CNCP)加入巴沙鱼糜,通过两段加热法制成CNCP-巴沙鱼糜复合凝胶。考察不同CNCP添加量(0%、0.2%、0.4%、0.6%、0.8%、1.0%)对CNCP-巴沙鱼糜复合凝胶的白度值、凝胶强度、质构特性、感官评价、蒸煮损失率、持水性、水分分布、动态流变学特性、凝胶形成作用力、蛋白质二级结构、微观结构的影响。结果表明:随着CNCP添加量的增多,凝胶性能呈先升后降的趋势,并在添加量为0.4%时达到峰值。此时,鱼糜凝胶强度为202.59 g·cm,硬度、感官评分、持水性相比对照组分别提升20.1%、35.02%、23.2%,具有最高的储能模量(G′),自由水含量仅为2.3%,不易流动水的弛豫时间显著缩短,蒸煮损失率显著降低,二硫键含量显著增加(P<0.05),α-螺旋向更稳定的β-折叠结构转变,此时微观结构孔隙最小,分形维数最大,凝胶网状结构最为致密。综上所述,CNCP的添加有助于与巴沙鱼糜形成致密的复合凝胶网络,从而改善鱼糜凝胶品质,为进一步开发优质健康的鱼糜产品提供新的思路。
