Mulberry anthocyanin,a natural flavonoid pigment,exhibits strong antioxidant activity with potential to mitigate chronic diseases.However,its specific molecular targets and mechanisms remain unclear.This study investi...Mulberry anthocyanin,a natural flavonoid pigment,exhibits strong antioxidant activity with potential to mitigate chronic diseases.However,its specific molecular targets and mechanisms remain unclear.This study investigated its antioxidant potential by integrating network pharmacology and computational modeling.Analysis identified tumor necrosis factor-alpha(TNF)and prostaglandin-endoperoxide synthase 2(PTGS2/COX-2)as key targets.Network pharmacology and pharmacophore screening pinpointed pelargonidin-3-O-rutinoside(Pg3R)as a key anthocyanin with dual inhibitory activity against these targets.Functional enrichment revealed their roles in lipid homeostasis,atherosclerosis,and extracellular matrix signaling under oxidative stress.Molecular docking and dynamics simulations confirmed that Pg3R forms stable hydrogen-bond networks and hydrophobic interfaces with high binding affinity and thermodynamic stability.Pharmacokinetic and toxicological evaluations indicated good bioavailability and no significant metabolic risks,though limited oral absorption suggests need for structural optimization.This study elucidates the antioxidant mechanism of mulberry anthocyanins and identifies Pg3R as a promising dual-targeting lead compound.The findings support its development for treating oxidative stress-related diseases and its use as a functional food additive.展开更多
为分析控制器局域网(Controller Area Network,CAN)协议,评价CAN网络性能,提出在OPNET网络仿真环境中CAN网络模型的建模方法.采用网络层次化建模方法构建节点模型.介质访问控制(Medium Access Control,MAC)子层中设计MAC、错误处理和帧...为分析控制器局域网(Controller Area Network,CAN)协议,评价CAN网络性能,提出在OPNET网络仿真环境中CAN网络模型的建模方法.采用网络层次化建模方法构建节点模型.介质访问控制(Medium Access Control,MAC)子层中设计MAC、错误处理和帧间空间模块,保证系统结构清晰.通过进程设计,MAC模块中实现了网络实时性模型;错误处理模块中实现了连续错误下网络不可用模型.定义和编辑链路及数据帧模型.根据实例模型进行网络模型的配置和仿真.仿真结果与实例测试结果一致,验证了网络模型的有效性.通过分析具体网络仿真环境下的仿真结果,对CAN网络性能进行了讨论.该模型提供了独立可编辑的节点模型,链路模型和数据帧模型,可实现灵活的网络配置,用于不同网络拓扑分析.展开更多
基金funded by National Natural Science Foundation of China(No.32302687)ChinaGraduate Research&Practice Innovation Program of Jiangsu Province,Jiangsu,China(No.KYCX24_4145).
文摘Mulberry anthocyanin,a natural flavonoid pigment,exhibits strong antioxidant activity with potential to mitigate chronic diseases.However,its specific molecular targets and mechanisms remain unclear.This study investigated its antioxidant potential by integrating network pharmacology and computational modeling.Analysis identified tumor necrosis factor-alpha(TNF)and prostaglandin-endoperoxide synthase 2(PTGS2/COX-2)as key targets.Network pharmacology and pharmacophore screening pinpointed pelargonidin-3-O-rutinoside(Pg3R)as a key anthocyanin with dual inhibitory activity against these targets.Functional enrichment revealed their roles in lipid homeostasis,atherosclerosis,and extracellular matrix signaling under oxidative stress.Molecular docking and dynamics simulations confirmed that Pg3R forms stable hydrogen-bond networks and hydrophobic interfaces with high binding affinity and thermodynamic stability.Pharmacokinetic and toxicological evaluations indicated good bioavailability and no significant metabolic risks,though limited oral absorption suggests need for structural optimization.This study elucidates the antioxidant mechanism of mulberry anthocyanins and identifies Pg3R as a promising dual-targeting lead compound.The findings support its development for treating oxidative stress-related diseases and its use as a functional food additive.
文摘为分析控制器局域网(Controller Area Network,CAN)协议,评价CAN网络性能,提出在OPNET网络仿真环境中CAN网络模型的建模方法.采用网络层次化建模方法构建节点模型.介质访问控制(Medium Access Control,MAC)子层中设计MAC、错误处理和帧间空间模块,保证系统结构清晰.通过进程设计,MAC模块中实现了网络实时性模型;错误处理模块中实现了连续错误下网络不可用模型.定义和编辑链路及数据帧模型.根据实例模型进行网络模型的配置和仿真.仿真结果与实例测试结果一致,验证了网络模型的有效性.通过分析具体网络仿真环境下的仿真结果,对CAN网络性能进行了讨论.该模型提供了独立可编辑的节点模型,链路模型和数据帧模型,可实现灵活的网络配置,用于不同网络拓扑分析.
