[Objectives]To investigate the chemical composition,antioxidant property,and nematicidal activity of Cucumis metuliferus and establish a theoretical foundation for the development of botanical nematicides.[Methods]The...[Objectives]To investigate the chemical composition,antioxidant property,and nematicidal activity of Cucumis metuliferus and establish a theoretical foundation for the development of botanical nematicides.[Methods]The compounds were isolated and purified using silica gel,reversed-phase medium,low-pressure gel Sephadex LH-20 column chromatography,and preparative high-performance liquid chromatography.Their identification was based on physicochemical properties,various spectral analyses,and data reported in the literature.Subsequently,the nematicidal and antioxidant activities of the isolated compounds were evaluated.[Results]A total of four compounds were isolated from C.metuliferus,which were identified as isovitexin-2″-O-glucoside(compound 1),9E,11E-(13-oxo)tridecadienoic acid(compound 2),2E,4E-tridecadienedioic acid(compound 3),and 9E,11E-(13-hydroxy)tridecadienoic acid(compound 4).Compound 1 demonstrated a notable degree of antioxidant activity,achieving a DPPH scavenging rate of 49.40%at a mass concentration of 1 mg/mL.Additionally,compounds 1,3,and 4 exhibited varying levels of nematicidal activity.Specifically,compound 1 displayed strong nematicidal activity at a concentration of 2.0 mg/mL,resulting in a mortality rate of 90.5%against nematodes.Furthermore,compounds 3 and 4 achieved a mortality rate of 100%against nematodes at a concentration of 0.25 mg/mL.[Conclusions]This study reports the first isolation of four compounds:isovitexin-2″-O-glucoside,9E,11E-(13-oxo)tridecadienoic acid,2E,4E-tridecadienedioic acid,and 9E,11E-(13-hydroxy)tridecadienoic acid from C.metuliferus.Notably,2E,4E-tridecadienedioic acid and 9E,11E-(13-oxo)tridecadienoic acid are identified as novel natural products.Among these compounds,isovitexin-2″-O-glucoside,9E,11E-(13-oxo)tridecadienoic acid,and 9E,11E-(13-hydroxy)tridecadienoic acid exhibit nematicidal activity,whereas isovitexin-2″-O-glucoside demonstrates antioxidant properties.展开更多
基金Supported by Natural Science Foundation Project of Xinjiang Uygur Autonomous Region(2021D01B59)Youth Science and Technology Backbone Project of Xinjiang Academy of Agricultural Sciences(XJNKQ-2021011)+2 种基金General Program of Hainan Natural Science Foundation(319MS109)Major Science and Technology Program of Hainan Province(ZDKJ2021005)Specific Research Fund of the Innovation Platform for Academicians of Hainan Province(YSPTZX202141).
文摘[Objectives]To investigate the chemical composition,antioxidant property,and nematicidal activity of Cucumis metuliferus and establish a theoretical foundation for the development of botanical nematicides.[Methods]The compounds were isolated and purified using silica gel,reversed-phase medium,low-pressure gel Sephadex LH-20 column chromatography,and preparative high-performance liquid chromatography.Their identification was based on physicochemical properties,various spectral analyses,and data reported in the literature.Subsequently,the nematicidal and antioxidant activities of the isolated compounds were evaluated.[Results]A total of four compounds were isolated from C.metuliferus,which were identified as isovitexin-2″-O-glucoside(compound 1),9E,11E-(13-oxo)tridecadienoic acid(compound 2),2E,4E-tridecadienedioic acid(compound 3),and 9E,11E-(13-hydroxy)tridecadienoic acid(compound 4).Compound 1 demonstrated a notable degree of antioxidant activity,achieving a DPPH scavenging rate of 49.40%at a mass concentration of 1 mg/mL.Additionally,compounds 1,3,and 4 exhibited varying levels of nematicidal activity.Specifically,compound 1 displayed strong nematicidal activity at a concentration of 2.0 mg/mL,resulting in a mortality rate of 90.5%against nematodes.Furthermore,compounds 3 and 4 achieved a mortality rate of 100%against nematodes at a concentration of 0.25 mg/mL.[Conclusions]This study reports the first isolation of four compounds:isovitexin-2″-O-glucoside,9E,11E-(13-oxo)tridecadienoic acid,2E,4E-tridecadienedioic acid,and 9E,11E-(13-hydroxy)tridecadienoic acid from C.metuliferus.Notably,2E,4E-tridecadienedioic acid and 9E,11E-(13-oxo)tridecadienoic acid are identified as novel natural products.Among these compounds,isovitexin-2″-O-glucoside,9E,11E-(13-oxo)tridecadienoic acid,and 9E,11E-(13-hydroxy)tridecadienoic acid exhibit nematicidal activity,whereas isovitexin-2″-O-glucoside demonstrates antioxidant properties.
