The exploration and understanding of myxosporean,one of the crucial stressors causing fish disease,have always been highly regarded.The present study aims to fully understand a novel myxobolid species,Thelohanellus xi...The exploration and understanding of myxosporean,one of the crucial stressors causing fish disease,have always been highly regarded.The present study aims to fully understand a novel myxobolid species,Thelohanellus xiushanensis n.sp.infecting the gill filaments of Carassius auratus from Chongqing,China,as well as the associated pathological impacts.A multifaceted analysis at various levels and perspectives was carried out by the approach integrating morphology,molecular systematics,histopathology,combined host and habitat,as well as SSU rDNA data.The results indicated that its mature myxospores displayed a tapering anterior and a blunt-rounded posterior,with a dropshape in front view and melon-seed shape in sutural view in morphology.The outer surface was covered with a transparent membrane sheath in valve view.The dimensions of myxospores were average(19.2±1.1)(17.1-21.0)×(10.0±0.7)(8.7-12.1)×(9.7±0.5)(9.1-10.8)μm.An elliptical polar capsule,containing four to five turns of single polar filament,measures(8.0±0.6)(6.9-9.1)×(6.6±0.3)(6.1-7.1)μm.Its SSU rDNA sequence demonstrated the closest similarity(99.95%)with an actinosporean Neoactinomyxum sp.NEO-7.Histopathological results indicated that numerous myxoplasmodia of T.xiushanensis n.sp.were located in the tips of the gill filaments,resulting in deformation and rupture of partial gill filaments.Furthermore,severely infected gill lamellae exhibited hyperplasia and fusion,accompanied by infiltration of numerous eosinophils.In addition,regenerated gill filaments were observed at the bases of partially ruptured gill filaments,indicating that the host’s gill tissue has the innate capacity to shed diseased tissues and regenerate new ones.This study has proved the present species and Neoactinomyxum sp.NEO-7 belong to the same myxosporean species but corresponding to different developmental stage,designated as a new species.This novel species is a potential pathogen for the host,which should not be ignored.This study not only enriches myxobolid biodiversity and demonstrates a potentially pathogenic agent,but also provides a valid diagnosis for the gill myxoboliosis caused by the present pathogen in aquaculture.展开更多
Otiorhynchus oliveri,n.sp.of Curculionid from Spain and notes on some taxa of the O.andarensis Reitter,1913,group.The new species belongs to the group of O.andarensis Reitter and ischaracterized by the deeper and dens...Otiorhynchus oliveri,n.sp.of Curculionid from Spain and notes on some taxa of the O.andarensis Reitter,1913,group.The new species belongs to the group of O.andarensis Reitter and ischaracterized by the deeper and denser punctures,the male metatibia lacking spinose processes and genitalia.Type locality:Catòute Mount,in Leon province,on rocky limestone outcrops at 1,900 metres a.s.l.Someremarks on the chorology and bioecology of other species of the group are also provided.展开更多
在福建省泉州市洛江区的湿地松（Pinus elliottii）上发现长尾属一新种,命名为福建长尾线虫（Seinura fujianensis n. sp.）.福建长尾线虫与李氏长尾线虫（Seinuralii）最为相似,两者的主要区别是：前者雌虫热杀死后尾部微向腹面弯曲,...在福建省泉州市洛江区的湿地松（Pinus elliottii）上发现长尾属一新种,命名为福建长尾线虫（Seinura fujianensis n. sp.）.福建长尾线虫与李氏长尾线虫（Seinuralii）最为相似,两者的主要区别是：前者雌虫热杀死后尾部微向腹面弯曲,后者弯曲明显;前者口针较短（雌虫分别为13-15μm,16.3~20.1μm,雄虫分别为12-15μm,16.8~18.7μm）,口针基部微膨大,后者口针无口针基部球,亦不膨大;前者雄虫尾部较短（分别为26-36μm,48~64μm）,c′值较小（分别为1.6-2.5,2.7-3.6）.展开更多
Ciliates of the heterotrich family Folliculinidae are characterized by their transparent lorica,conspicuous peristomial lobes pair and dimorphic life cycle,a sessile trophont and a motile swarmer.However,understanding...Ciliates of the heterotrich family Folliculinidae are characterized by their transparent lorica,conspicuous peristomial lobes pair and dimorphic life cycle,a sessile trophont and a motile swarmer.However,understanding their biodiversity and systematics is relatively poor.In the present study,two species representing two genera of folliculinids,which were collected from marine and brackish habitats in China,were investigated with morphological and molecular methods.The genus Diafolliculina n.gen.is established for D.longilobata n.sp.Detailed morphological redescriptions for another species Eufolliculina is provided.Moreover,phylogenetic analysis based on small subunit ribosomal DNA(SSU rDNA)sequences showed that 1)all two genera locate in the Folliculinidae clade;2)Metafolliculina clusters with Eufolliculina;and 3)two genera(Diafolliculina and Ampullofolliculina)with a lorica closure device have close affinities with Folliculina.The lorica of Diafolliculina and Ampullofolliculina lacks a closure device,thereby the phylogeney contradicts the morphology-based classification.展开更多
Objective:To study nematode parasites morphology of Hystrix javanica(H.javanica),both through the feces and internal organs.Methods:Feces were observed by direct smear method,internal organs were observed after dissec...Objective:To study nematode parasites morphology of Hystrix javanica(H.javanica),both through the feces and internal organs.Methods:Feces were observed by direct smear method,internal organs were observed after dissecting the host.Specimens for light microscopy examination were fixed with 70%warm alcohol,cleared and mounted in lactophenol for wet mounting.Specimens for SEM examination were postfixed in cacodylate buffer and glutaraldehyde,dehydrated through a graded series of alcohol and freeze dried.The specimens were attached to stubs with double cello-tape,coated with gold and observed with a JSM5310LV electron microscope.Figures were made with the aid of a drawiug tube attached to Olympus compound microscope,other figures were photographs of scanning electron microscope images.Measurements were given in micrometers as the mean followed by the range in parentheses,unless otherwise stated.Results:The nematode species found in the intestine of H.javanica are Gireterakis girardi and a new species,Trihuris landak.The new species differs with previously reported species from Hystrix because of having stylet and short cervical alae.The pattern of bacillary band is closed to Trichuris trichiurus,the species that infect human,but differs because the surface of its vulva is not covered with densely spine.Conclusions:The species of nematodes found on H.javanica were Gireterakis girardi and a new species Trichuris landak n.sp.Those two species are newly recorded in Indonesia.展开更多
基金National Natural Science Foundation of China(31970409)Projects of Chongqing Science and Technology Commission(cstc2021jcyj-msxmX0731)。
文摘The exploration and understanding of myxosporean,one of the crucial stressors causing fish disease,have always been highly regarded.The present study aims to fully understand a novel myxobolid species,Thelohanellus xiushanensis n.sp.infecting the gill filaments of Carassius auratus from Chongqing,China,as well as the associated pathological impacts.A multifaceted analysis at various levels and perspectives was carried out by the approach integrating morphology,molecular systematics,histopathology,combined host and habitat,as well as SSU rDNA data.The results indicated that its mature myxospores displayed a tapering anterior and a blunt-rounded posterior,with a dropshape in front view and melon-seed shape in sutural view in morphology.The outer surface was covered with a transparent membrane sheath in valve view.The dimensions of myxospores were average(19.2±1.1)(17.1-21.0)×(10.0±0.7)(8.7-12.1)×(9.7±0.5)(9.1-10.8)μm.An elliptical polar capsule,containing four to five turns of single polar filament,measures(8.0±0.6)(6.9-9.1)×(6.6±0.3)(6.1-7.1)μm.Its SSU rDNA sequence demonstrated the closest similarity(99.95%)with an actinosporean Neoactinomyxum sp.NEO-7.Histopathological results indicated that numerous myxoplasmodia of T.xiushanensis n.sp.were located in the tips of the gill filaments,resulting in deformation and rupture of partial gill filaments.Furthermore,severely infected gill lamellae exhibited hyperplasia and fusion,accompanied by infiltration of numerous eosinophils.In addition,regenerated gill filaments were observed at the bases of partially ruptured gill filaments,indicating that the host’s gill tissue has the innate capacity to shed diseased tissues and regenerate new ones.This study has proved the present species and Neoactinomyxum sp.NEO-7 belong to the same myxosporean species but corresponding to different developmental stage,designated as a new species.This novel species is a potential pathogen for the host,which should not be ignored.This study not only enriches myxobolid biodiversity and demonstrates a potentially pathogenic agent,but also provides a valid diagnosis for the gill myxoboliosis caused by the present pathogen in aquaculture.
文摘Otiorhynchus oliveri,n.sp.of Curculionid from Spain and notes on some taxa of the O.andarensis Reitter,1913,group.The new species belongs to the group of O.andarensis Reitter and ischaracterized by the deeper and denser punctures,the male metatibia lacking spinose processes and genitalia.Type locality:Catòute Mount,in Leon province,on rocky limestone outcrops at 1,900 metres a.s.l.Someremarks on the chorology and bioecology of other species of the group are also provided.
文摘在福建省泉州市洛江区的湿地松（Pinus elliottii）上发现长尾属一新种,命名为福建长尾线虫（Seinura fujianensis n. sp.）.福建长尾线虫与李氏长尾线虫（Seinuralii）最为相似,两者的主要区别是：前者雌虫热杀死后尾部微向腹面弯曲,后者弯曲明显;前者口针较短（雌虫分别为13-15μm,16.3~20.1μm,雄虫分别为12-15μm,16.8~18.7μm）,口针基部微膨大,后者口针无口针基部球,亦不膨大;前者雄虫尾部较短（分别为26-36μm,48~64μm）,c′值较小（分别为1.6-2.5,2.7-3.6）.
基金This work was supported by the National Natural Science Foundation of China(No.31970398)the National Key Research and Development Program of China(No.2018YFD0900701)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(No.2019333).
文摘Ciliates of the heterotrich family Folliculinidae are characterized by their transparent lorica,conspicuous peristomial lobes pair and dimorphic life cycle,a sessile trophont and a motile swarmer.However,understanding their biodiversity and systematics is relatively poor.In the present study,two species representing two genera of folliculinids,which were collected from marine and brackish habitats in China,were investigated with morphological and molecular methods.The genus Diafolliculina n.gen.is established for D.longilobata n.sp.Detailed morphological redescriptions for another species Eufolliculina is provided.Moreover,phylogenetic analysis based on small subunit ribosomal DNA(SSU rDNA)sequences showed that 1)all two genera locate in the Folliculinidae clade;2)Metafolliculina clusters with Eufolliculina;and 3)two genera(Diafolliculina and Ampullofolliculina)with a lorica closure device have close affinities with Folliculina.The lorica of Diafolliculina and Ampullofolliculina lacks a closure device,thereby the phylogeney contradicts the morphology-based classification.
文摘Objective:To study nematode parasites morphology of Hystrix javanica(H.javanica),both through the feces and internal organs.Methods:Feces were observed by direct smear method,internal organs were observed after dissecting the host.Specimens for light microscopy examination were fixed with 70%warm alcohol,cleared and mounted in lactophenol for wet mounting.Specimens for SEM examination were postfixed in cacodylate buffer and glutaraldehyde,dehydrated through a graded series of alcohol and freeze dried.The specimens were attached to stubs with double cello-tape,coated with gold and observed with a JSM5310LV electron microscope.Figures were made with the aid of a drawiug tube attached to Olympus compound microscope,other figures were photographs of scanning electron microscope images.Measurements were given in micrometers as the mean followed by the range in parentheses,unless otherwise stated.Results:The nematode species found in the intestine of H.javanica are Gireterakis girardi and a new species,Trihuris landak.The new species differs with previously reported species from Hystrix because of having stylet and short cervical alae.The pattern of bacillary band is closed to Trichuris trichiurus,the species that infect human,but differs because the surface of its vulva is not covered with densely spine.Conclusions:The species of nematodes found on H.javanica were Gireterakis girardi and a new species Trichuris landak n.sp.Those two species are newly recorded in Indonesia.
