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Multi-objective optimization of gas metal arc welding parameters and sequences for low-carbon steel (Q345D) T-joints 被引量:7
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作者 Qing Shao Tao Xu +1 位作者 Tatsuo Yoshino Nan Song 《Journal of Iron and Steel Research International》 SCIE EI CAS CSCD 2017年第5期544-555,共12页
Q345D high-quality low-carbon steel has been extensively employed in structures with stringent weld- ing quality requirements. A multi-objective optimization of welding stress and deformation was presented to design r... Q345D high-quality low-carbon steel has been extensively employed in structures with stringent weld- ing quality requirements. A multi-objective optimization of welding stress and deformation was presented to design reasonable values of gas metal arc welding parameters and sequences of Q345D T-joints. The optimized factors included continuous variables (welding current (I), welding voltage (U) ahd welding speed (V)) and discrete variables (welding sequence (S) and welding direc- tion (D)). The concepts of the pointer and stack in Visual Basic (VB) and the interpolation method were introduced to optimize the variables. The optimization objectives included the different combina- tions of the angular distortion and transverse welding stress along the transverse and longitudinal dis- tributions. Based on the design of experiments (DOE) and the polynomial regression (PR) model, the finite element (FE) results of the T-joint were used to establish the mathematical models. The Pareto front and the compromise solutions were obtained by using a multi-objective particle swarm optimization (MOPSO) algorithm. The optimal results were validated by the corresponding results of the FE method, and the error between the FE results and the two-objective results as well as that be-tween the FE results and the three-objective optimization results were less than 17.2% and 21.5%, respectively. The influence and setting regularity of different factors were discussed according to the compromise solutions. 展开更多
关键词 T-JOINT Welding parameter Welding sequence multi-objective optimization Pareto front Gas metal arc welding Q345D
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Sequencing Mixed-model Production Systems by Modified Multi-objective Genetic Algorithms 被引量:5
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作者 WANG Binggang 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2010年第5期537-546,共10页
As two independent problems,scheduling for parts fabrication line and sequencing for mixed-model assembly line have been addressed respectively by many researchers.However,these two problems should be considered simul... As two independent problems,scheduling for parts fabrication line and sequencing for mixed-model assembly line have been addressed respectively by many researchers.However,these two problems should be considered simultaneously to improve the efficiency of the whole fabrication/assembly systems.By far,little research effort is devoted to sequencing problems for mixed-model fabrication/assembly systems.This paper is concerned about the sequencing problems in pull production systems which are composed of one mixed-model assembly line with limited intermediate buffers and two flexible parts fabrication flow lines with identical parallel machines and limited intermediate buffers.Two objectives are considered simultaneously:minimizing the total variation in parts consumption in the assembly line and minimizing the total makespan cost in the fabrication/assembly system.The integrated optimization framework,mathematical models and the method to construct the complete schedules for the fabrication lines according to the production sequences for the first stage in fabrication lines are presented.Since the above problems are non-deterministic polynomial-hard(NP-hard),a modified multi-objective genetic algorithm is proposed for solving the models,in which a method to generate the production sequences for the fabrication lines from the production sequences for the assembly line and a method to generate the initial population are put forward,new selection,crossover and mutation operators are designed,and Pareto ranking method and sharing function method are employed to evaluate the individuals' fitness.The feasibility and efficiency of the multi-objective genetic algorithm is shown by computational comparison with a multi-objective simulated annealing algorithm.The sequencing problems for mixed-model production systems can be solved effectively by the proposed modified multi-objective genetic algorithm. 展开更多
关键词 mixed-model production system sequencING parallel machine BUFFERS multi-objective genetic algorithm multi-objective simulated annealing algorithm
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Mtuli-objective Optimization Method for Automatic Drilling and Riveting Sequence Planning 被引量:3
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作者 Xiao Hong Li Yuan +3 位作者 Zhang Kaifu Yu Jianfeng Liu Zhenxing Su Jianbin 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2010年第6期734-742,共9页
There are numerous riveting points on the large-sized aircraft panel, irregular row of riveting points on delta wing. It is essential to plan the riveting sequence reasonably to improve the efficiency and accuracy of ... There are numerous riveting points on the large-sized aircraft panel, irregular row of riveting points on delta wing. It is essential to plan the riveting sequence reasonably to improve the efficiency and accuracy of automatic drilling and riveting. Therefore, this article presents a new multi-objective optimization method based on ant colony optimization (ACO). Multi-objective optimization model of automatic drilling and riveting sequence planning is built by expressing the efficiency and accuracy of riveting as functions of the points' coordinates. In order to search the sequences efficiently and improve the quality of the sequences, a new local pheromone updating rule is applied when the ants search sequences. Pareto dominance is incorporated into the proposed ACO to find out the non-dominated sequences. This method is tested on a hyperbolicity panel model of ARJ21 and the result shows its feasibility and superiority compared with particle swarm optimization (PSO) and genetic algorithm (GA). 展开更多
关键词 automatic drilling and riveting riveting sequence multi-objective optimization ant colony optimization Paretooptimal solutions
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Review of techniques for motion capture data processing 被引量:3
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作者 Wei Xiaopeng Liu Rul and Zhang Qiang 《Computer Aided Drafting,Design and Manufacturing》 2012年第1期1-11,共11页
In order to high reality and efficiency, the technique computer animation. With the development of motion capture, a of motion capture (MoCap) has been widely used in the field of large amount of motion capture data... In order to high reality and efficiency, the technique computer animation. With the development of motion capture, a of motion capture (MoCap) has been widely used in the field of large amount of motion capture databases are available and this is significant for the reuse of motion data. But due to the high degree of freedoms and high capture frequency, the dimension of the mo- tion capture data is usually very high and this will lead to a low efficiency in data processing. So how to process the high dimension data and design an efficient and effective retrieval approach has become a challenge which we can't ignore. In this paper, first we lay out some problems about the key techniques in motion capture data processing. Then the existing approaches are analyzed and sum- marized. At last, some future work is proposed. 展开更多
关键词 3D motion capture motion sequence segmentation key-frame extraction motion retrieval
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Particle Swarm Optimization Algorithm Based on Chaotic Sequences and Dynamic Self-Adaptive Strategy
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作者 Mengshan Li Liang Liu +4 位作者 Genqin Sun Keming Su Huaijin Zhang Bingsheng Chen Yan Wu 《Journal of Computer and Communications》 2017年第12期13-23,共11页
To deal with the problems of premature convergence and tending to jump into the local optimum in the traditional particle swarm optimization, a novel improved particle swarm optimization algorithm was proposed. The se... To deal with the problems of premature convergence and tending to jump into the local optimum in the traditional particle swarm optimization, a novel improved particle swarm optimization algorithm was proposed. The self-adaptive inertia weight factor was used to accelerate the converging speed, and chaotic sequences were used to tune the acceleration coefficients for the balance between exploration and exploitation. The performance of the proposed algorithm was tested on four classical multi-objective optimization functions by comparing with the non-dominated sorting genetic algorithm and multi-objective particle swarm optimization algorithm. The results verified the effectiveness of the algorithm, which improved the premature convergence problem with faster convergence rate and strong ability to jump out of local optimum. 展开更多
关键词 Particle SWARM Algorithm CHAOTIC sequenceS SELF-ADAPTIVE STRATEGY multi-objectIVE Optimization
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Conductor Arrangement and Phase Sequence Optimization Scheme for 500 kV Four-Circuit Transmission Lines on Same Tower
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作者 Deng Lu Xujun Lang +2 位作者 Bo Yang Ziyang Li Hang Geng 《Energy Engineering》 EI 2023年第10期2287-2306,共20页
The four-circuit parallel line on the same tower effectively solves the problems faced by the line reconstruction and construction under the condition of the increasing shortage of transmission corridors.Optimizing th... The four-circuit parallel line on the same tower effectively solves the problems faced by the line reconstruction and construction under the condition of the increasing shortage of transmission corridors.Optimizing the conductor and phase sequence arrangement of multiple transmission lines is conducive to improving electromagnetic and electrostatic coupling caused by electromagnetic problems.This paper uses the ATP-EMTP simulation software to build a 500 kV multi-circuit transmission line on the same tower.It stimulates the induced voltage and current values of different line lengths,tower spacing,vertical and horizontal spacing between different circuits,phase sequence arrangement,and nominal tower height.Moreover,use the BP neural network optimized by a genetic algorithm to predict the induced voltage and current under the unknown conductor and phase sequence arrangement.Finally,based on multi-objective particle swarm algorithm to construct the optimization model of conductor arrangement scheme of overhead transmission line,combined with electromagnetic environment control index,determine the optimal conductor arrangement scheme by the size of particle fitness function,a significant reduction in induced voltages and currents between transmission lines and the four-circuit conductor layout scheme meeting the requirements of the electromagnetic environment is obtained,which provides a reference for the tower design of the transmission station project. 展开更多
关键词 Spatial arrangement phase sequence arrangement genetic algorithm BP neural network multi-objective particle swarm algorithm
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Transfer ionization in isocharge sequence ion and Ne collisions
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作者 CAIXiao-hong YUDe-yang LURong-chun CAOZhu-rong YANGWei SHAOCao-jie 《原子与分子物理学报》 CAS CSCD 北大核心 2004年第B04期130-132,共3页
The dependence of the ratio R1 for transfer ionization to single capture for Cq+, Nq+, Oq+, Neq+ ions on Ne target upon the electronic structure of the projectile is studied. For Aq+-Ne collisions the ratio R1 decreas... The dependence of the ratio R1 for transfer ionization to single capture for Cq+, Nq+, Oq+, Neq+ ions on Ne target upon the electronic structure of the projectile is studied. For Aq+-Ne collisions the ratio R1 decreases as the atomic number Z of the projectile increases for q=4,5,6,7 sequences which provides strong evidence for the increase of the binding energy of the target valence electron after single electron capture. The increase in binding energy depends both upon the atomic number of the projectile and the target atom. 展开更多
关键词 电子俘获 迁移电离作用 氖碰撞 等电子离子 原子物理学
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基于Alltype^(TM)Fastplex^(TM)二代测序的HLA-B等位基因漏检原因分析
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作者 全湛柔 杨冰娜 +1 位作者 刘洁 邹红岩 《中国实验血液学杂志》 北大核心 2026年第1期255-260,共6页
目的:探讨1例基于Alltype^(TM)Fastplex^(TM)二代测序(NGS)的HLA-B等位基因漏检情况,以评估NGS在HLA基因分型中的准确性和局限性。方法:应用PCR-序列特异性寡核苷酸探针(SSOP)及Alltype^(TM)Fastplex^(TM)NGS进行HLA常规检测,发现1例HL... 目的:探讨1例基于Alltype^(TM)Fastplex^(TM)二代测序(NGS)的HLA-B等位基因漏检情况,以评估NGS在HLA基因分型中的准确性和局限性。方法:应用PCR-序列特异性寡核苷酸探针(SSOP)及Alltype^(TM)Fastplex^(TM)NGS进行HLA常规检测,发现1例HLA-B位点结果不一致的血小板无力症患者样本后,采用PCR-直接测序法(SBT)及AlloSeq Tx17杂交捕获的NGS技术对该样本进行复检,以验证Alltype^(TM)Fastplex^(TM)NGS的检测结果。结果:SSOP分型结果显示该患者HLA-B位点为B*15:01,40:01,而Alltype^(TM)Fastplex^(TM)NGS结果分析为B*40:01,40:01纯合子。为了确认NGS的检测结果,本研究对该纯合子样本进行了SBT和AlloSeq Tx17杂交捕获NGS复检。SBT分析结果显示,存在两种可能的组合,分别是B*15:01,40:01和B*15:07,40:01,表明SBT结果模棱两可。而AlloSeq Tx17 NGS结果则明确显示为B*15:01,40:01,与SSOP分型结果一致,从而确认了Alltype^(TM)Fastplex^(TM)NGS在HLA-B位点漏检了B*15:01等位基因。结论:扩增子文库构建的NGS法存在HLA等位基因漏检的局限性,特别是对于检出的纯合子样本,应采用多种实验方法进行复核,保证HLA基因分型结果的准确性。 展开更多
关键词 HLA-B 基因分型 扩增子 杂交捕获 二代测序
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基于脉冲耦合神经网络的滚动轴承振动故障诊断方法研究
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作者 艾辛平 乔永利 +2 位作者 申礼 姚玉东 田晶 《航天制造技术》 2026年第1期70-78,共9页
滚动轴承作为航空发动机转子和火箭发动机转轴的重要零件,因工作条件苛刻而故障多发,且危害较大,对其进行准确的故障诊断具有重要意义。首先,对脉冲耦合神经网络(PCNN)进行简化和改进,分析其脉冲耦合特性,建立了基于线性衰减权重动态门... 滚动轴承作为航空发动机转子和火箭发动机转轴的重要零件,因工作条件苛刻而故障多发,且危害较大,对其进行准确的故障诊断具有重要意义。首先,对脉冲耦合神经网络(PCNN)进行简化和改进,分析其脉冲耦合特性,建立了基于线性衰减权重动态门限的二值图像一维化处理方法,提出了以各故障捕获比序列作为滚动轴承振动故障特征。然后,通过设置PCNN的相关参数,探究耦合连接强度对类间方差的影响,确定出耦合连接强度取值,并进行敏感性分析;采用局部线性嵌入算法(LLE)对高维捕获比序列进行降维,以故障样本集间的欧式距离为判据,确定出最优嵌入维度与最近邻样本个数。最后,利用PCNN进行特征提取和LLE降维,分别采用KNN、RF、Adaboost 3种分类器对4种轴承故障进行诊断与比较,进一步提高了轴承故障诊断的正确率,为航空和火箭发动机滚动轴承故障诊断提供了一个有效的方法。 展开更多
关键词 时频分析 脉冲耦合神经网络 捕获比序列 局部线性嵌入 滚动轴承 故障诊断
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Modified NSGA-II for a Bi-Objective Job Sequencing Problem 被引量:1
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作者 Susmita Bandyopadhyay 《Intelligent Information Management》 2012年第6期319-329,共11页
This paper proposes a better modified version of a well-known Multi-Objective Evolutionary Algorithm (MOEA) known as Non-dominated Sorting Genetic Algorithm-II (NSGA-II). The proposed algorithm contains a new mutation... This paper proposes a better modified version of a well-known Multi-Objective Evolutionary Algorithm (MOEA) known as Non-dominated Sorting Genetic Algorithm-II (NSGA-II). The proposed algorithm contains a new mutation algorithm and has been applied on a bi-objective job sequencing problem. The objectives are the minimization of total weighted tardiness and the minimization of the deterioration cost. The results of the proposed algorithm have been compared with those of original NSGA-II. The comparison of the results shows that the modified NSGA-II performs better than the original NSGA-II. 展开更多
关键词 JOB sequencING multi-objective Evolutionary Algorithm (MOEA) NSGA-II (Non-Dominated Sorting Genetic Algorithm-II) TARDINESS DETERIORATION Cost
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基于探针捕获法的病原靶向高通量测序检测流程的建立和验证研究 被引量:2
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作者 李丽莉 任珊珊 +4 位作者 王嘉平 易玉婷 田超 刘东来 许四宏 《中国药事》 2025年第4期415-429,共15页
目的:研究基于探针捕获法的病原靶向高通量测序(tNGS)检测流程的建立和优化,以及自动化的实现,并对分析性能进行验证研究。方法:分析tNGS的影响因素,包括探针设计、生产质控、数据量和测序读长等,通过采用多种细菌、真菌、病毒和非典型... 目的:研究基于探针捕获法的病原靶向高通量测序(tNGS)检测流程的建立和优化,以及自动化的实现,并对分析性能进行验证研究。方法:分析tNGS的影响因素,包括探针设计、生产质控、数据量和测序读长等,通过采用多种细菌、真菌、病毒和非典型病原体等62种微生物构建的参考盘进行分析性能评价,同时比较手工操作与自动化流程在检出限、精密度和交叉污染等性能指标的差异。结果:62种微生物不同病原浓度的准确性、检出限、精密度、特异性及线性的结果显示随着探针覆盖度的提高,目标病原的检出限性能随之提高,且仍保持较高的特异性;经优化后的tNGS检测流程在准确性、检出限、精密度、特异性、线性等方面均表现出良好的分析性能;自动化流程在检出限性能保持一致的前提下,精密度和抗交叉污染性能均优于手工操作流程。结论:研究建立的探针捕获法tNGS检测流程具有良好的分析性能,为临床病原检测提供了新策略。未来进一步研究应着重于临床样本确认、自动化流程的全面验证,以推动该技术在临床实际工作中更好的应用。 展开更多
关键词 病原靶向高通量测序 探针捕获法 分析性能 自动化
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糖肾康调控脱氧核糖核酸甲基化减轻糖尿病肾病足细胞焦亡的机制
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作者 周乐 高冉冉 +3 位作者 韩聪 王一川 鲍孝云 李伟 《世界中医药》 北大核心 2025年第11期1906-1911,1922,共7页
目的:探讨糖肾康通过调控DNA甲基化和足细胞焦亡改善糖尿病肾病(DKD)的机制。方法:采用30 mmol/L葡萄糖培养基诱导小鼠足细胞(MPC5)48 h建立足细胞损伤模型,制备糖肾康大鼠含药血清、采用DNA甲基化抑制剂(5-Aza-CdR)干预足细胞,观察细... 目的:探讨糖肾康通过调控DNA甲基化和足细胞焦亡改善糖尿病肾病(DKD)的机制。方法:采用30 mmol/L葡萄糖培养基诱导小鼠足细胞(MPC5)48 h建立足细胞损伤模型,制备糖肾康大鼠含药血清、采用DNA甲基化抑制剂(5-Aza-CdR)干预足细胞,观察细胞焦亡炎症介质Nod样受体蛋白3(NLRP3)、半胱氨酸天冬氨酸蛋白水解酶1(Caspase-1)、白细胞介素(IL-18)及IL-1β表达情况。采用高通量甲基化捕获测序筛选甲基化基因并结合网站预测,筛选出差异基因特异性蛋白1(Sp1)并进行实时定量PCR(qPCR)验证,构建并转染Sp1过表达质粒,观察糖肾康通过影响Sp1甲基化对足细胞焦亡的影响。结果:5-Aza-Cd可减少细胞焦亡炎症介质的表达(P<0.01)。结合甲基化测序、网站预测及qPCR验证,Sp1发生了甲基化且表达水平更高最具差异性,加入5-Aza-CdR后,Sp1表达明显减少(P<0.01),糖肾康对Sp1表达影响与5-Aza-CdR差异无统计学意义(P>0.05)。Sp1过表达后加入糖肾康或5-Aza-CdR均能减轻细胞焦亡因子的表达(P<0.01或P<0.05),且糖肾康的保护作用优于5-Aza-CdR(P<0.05)。结论:DNA甲基化可能通过调控足细胞焦亡参与了DKD进展,其机制可能是通过Sp1甲基化激活了细胞焦亡关键因子NLRP3炎性体,进一步通过NLRP3-Caspase-1-IL-1β/IL-18轴促进炎症介质表达,导致了足细胞焦亡,糖肾康或正是通过影响Sp1甲基化发挥足细胞保护作用,延缓DKD进展。 展开更多
关键词 糖尿病肾病 DNA甲基化 足细胞 细胞焦亡 高通量甲基化捕获测序 差异基因特异性蛋白1 糖肾康颗粒 益气活血清泄法
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生物炭强化高负荷接触稳定工艺碳捕获的效果研究
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作者 孙剑平 周佳迪 +1 位作者 李绍峰 刘婉怡 《安全与环境学报》 北大核心 2025年第6期2361-2371,共11页
为了进一步提升高负荷接触稳定法(High-rate Contact-Stabilization, HiCS)的碳捕获能力,开展了污泥基生物炭强化HiCS工艺碳捕获及其对微生物群落影响的研究,并通过多种分析手段考察生物炭投加量对HiCS系统的COD捕获效率和NH+4-N去除率... 为了进一步提升高负荷接触稳定法(High-rate Contact-Stabilization, HiCS)的碳捕获能力,开展了污泥基生物炭强化HiCS工艺碳捕获及其对微生物群落影响的研究,并通过多种分析手段考察生物炭投加量对HiCS系统的COD捕获效率和NH+4-N去除率的影响。同时,考察生物炭投加量对污泥性质、胞外聚合物和微生物丰度变化的影响。结果显示,投加60 mg/L生物炭的碳捕获率最高为58%,较对照组提高15百分点。NH+4-N的去除效率在生物炭为80 mg/L时达到最高,为55.1%,较对照组提高了29.9百分点。此外,生物炭作为载体可以富集大量功能微生物,提高整体的生物量,通过对污泥粒径及扫描电子显微镜观察发现,投加生物炭后的污泥粒径有所减小且粒径分布较为集中,且污泥表面产生较多孔隙并更加粗糙,这使得污泥具有更强的捕获能力。通过测定Zeta电位发现,随着生物炭投加量的增加,污泥的Zeta电位的绝对值在6~8 mV内逐渐减小,这表明污泥絮凝能力得到了提升。污泥的ρ(MLVSS)/ρ(MLSS)也随着生物炭的投加而增加,表明投加生物炭增强了系统内的生物活性。生物炭的投加也促进了胞外聚合物的分泌,高通量测序结果显示,生物炭会提高吸附、降解污染物相关的优势菌门的丰度,如变形菌门和拟杆菌门。 展开更多
关键词 环境工程学 高负荷接触稳定法 碳捕获 生物炭 高通量测序
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基于多目标序列捕捉与时空特征提取的电力人员行为识别算法
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作者 金浩远 闫云凤 +1 位作者 张春林 齐冬莲 《高电压技术》 北大核心 2025年第12期5788-5800,共13页
电力安全生产监管通常以人工督查方式为主,难以满足准确性、客观性和实时性的要求。随着计算机视觉技术的引入,智能识别方式得到了广泛关注。然而,现有基于单帧图像的电力人员行为识别算法,因未充分考虑运动连续性和上下文信息,不可避... 电力安全生产监管通常以人工督查方式为主,难以满足准确性、客观性和实时性的要求。随着计算机视觉技术的引入,智能识别方式得到了广泛关注。然而,现有基于单帧图像的电力人员行为识别算法,因未充分考虑运动连续性和上下文信息,不可避免地存在大量误检和漏检情况。因此,该文提出了一种基于多目标序列捕捉与时空特征提取的电力人员行为识别算法。该算法通过行为序列捕捉模块获得人员行为序列,并利用时空特征提取模块提取行为时空信息。该算法在公开数据集和电力数据集上完成了验证,在典型电力人员行为类别上的综合准确率达到88.6%以上。同时,其仍存在改进空间,可作为未来相关研究的基础框架。 展开更多
关键词 电力安全生产监管 计算机视觉 电力人员行为识别 多目标序列捕捉 时空特征提取
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High-performance single-chip exon capture allows accurate whole exome sequencing using the Illumina Genome Analyzer 被引量:3
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作者 JIANG Tao YANG Lei +2 位作者 JIANG Hui TIAN Geng ZHANG XiuQing 《Science China(Life Sciences)》 SCIE CAS 2011年第10期945-952,共8页
Here we present an adaptation of NimbleGen 2.1M-probe array sequence capture for whole exome sequencing using the Illumina Genome Analyzer (GA) platform.The protocol involves two-stage library construction.The specifi... Here we present an adaptation of NimbleGen 2.1M-probe array sequence capture for whole exome sequencing using the Illumina Genome Analyzer (GA) platform.The protocol involves two-stage library construction.The specificity of exome enrichment was approximately 80% with 95.6% even coverage of the 34 Mb target region at an average sequencing depth of 33-fold.Comparison of our results with whole genome shot-gun resequencing results showed that the exome SNP calls gave only 0.97% false positive and 6.27% false negative variants.Our protocol is also well suited for use with whole genome amplified DNA.The results presented here indicate that there is a promising future for large-scale population genomics and medical studies using a whole exome sequencing approach. 展开更多
关键词 whole exome sequencing exon capture SNP detection indel detection high-throughput resequencing
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基于混合策略的自适应红嘴蓝鹊优化算法
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作者 段博文 殷继彬 张航 《计算机科学》 北大核心 2025年第S2期127-136,共10页
针对红嘴蓝鹊优化算法(Red-billed Blue Magpie Optimization Algorithm,RBMO)存在多样性迅速退化、寻优精度差、易陷入局部最优的问题,提出了一种基于混合策略的自适应红嘴蓝鹊优化算法(Adaptive Red-Billed Blue Magpie Optimization ... 针对红嘴蓝鹊优化算法(Red-billed Blue Magpie Optimization Algorithm,RBMO)存在多样性迅速退化、寻优精度差、易陷入局部最优的问题,提出了一种基于混合策略的自适应红嘴蓝鹊优化算法(Adaptive Red-Billed Blue Magpie Optimization Algorithm Based on Mixed Strategy,JRBMO)。首先,引入Hammersley序列初始化种群,使初始解分布更均匀,为寻优提供基础;其次,在勘探阶段,提出自适应螺旋围捕策略,通过动态控制个体的勘探范围与方向,提高RBMO的搜索能力。在开发阶段,引入莱维飞行策略,对当前最优解进行局部扰动,增强算法局部开发能力;最后,提出自适应维度变异策略,根据种群适应度分布的变化,对个体进行维度变异,避免算法陷入局部最优。在CEC2017与CEC2019测试集上对算法性能进行评估,结果显示JRBMO均值胜率分别达到88.9%和70%,验证了JRBMO的有效性。此外,将JRBMO应用于拉(压)弹簧设计问题和三维无线传感器网络(WSN)节点覆盖问题上,JRBMO均取得了最优的结果,其中WSN节点均值覆盖率高出原算法6.3%,体现了JRBMO在实际应用中的普适性。 展开更多
关键词 红嘴蓝鹊优化算法 自适应 Hammersley序列 螺旋围捕 莱维飞行 维度变异
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两种二代测序技术在儿童中枢神经系统感染病原学检测中的应用
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作者 翁婷 王程毅 《福建医药杂志》 2025年第8期12-15,共4页
目的比较脑脊液靶向二代测序(targeted next-generation sequencing,tNGS)与宏基因捕获法(metagenomics capture,MetaCAP)二代测序技术在儿童中枢神经系统感染(central nervous system infection,CNSI)病原学检测中的价值。方法回顾性... 目的比较脑脊液靶向二代测序(targeted next-generation sequencing,tNGS)与宏基因捕获法(metagenomics capture,MetaCAP)二代测序技术在儿童中枢神经系统感染(central nervous system infection,CNSI)病原学检测中的价值。方法回顾性分析于福建省儿童医院救治的CNSI患儿69例,按脑脊液二代测序检测方式分为A组(MetaCAP组,n=21)和B组(tNGS组,n=48)。统计患儿临床资料,包括年龄、性别、基础疾病、传统微生物学检测(conventional microbiological tests,CMTs)结果、二代测序结果及影像学检查结果等,探讨两种测序技术在儿童CNSI病原学检测中的价值。结果tNGS与MetaCAP的检测周期均短于脑脊液培养(均P<0.05),tNGS阳性率不受采样前抗菌药物影响(P>0.05)。MetaCAP与tNGS阳性率均高于CMTs阳性率(均P<0.001)。两种NGS技术在病原体种类方面呈现互补特征:MetaCAP细菌检出率高于tNGS(P<0.05),而tNGS在病毒检出方面略有优势(39.6%vs.28.6%)。MetaCAP在耐药株检出和指导抗感染方案方面均较tNGS突出(均P<0.05)。结论MetaCAP和tNGS均为儿童CNSI诊断提供了重要补充,为精准诊疗提供了重要技术支持。 展开更多
关键词 中枢神经系统感染 宏基因捕获法二代测序 靶向二代测序 儿童
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宏基因捕获法二代测序技术在肺部感染病原学诊断中的价值
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作者 黄珊珠 朱红军 黄裔腾 《汕头大学医学院学报》 2025年第2期98-101,共4页
目的:探讨宏基因捕获法(metagenomics capture,MetaCAP)二代测序技术在肺部感染病原学诊断中的临床应用价值。方法:选取2024年1—12月汕头市中心医院122例肺部感染患者。所有患者均通过支气管镜获取肺泡灌洗液标本,并行MetaCAP检测和传... 目的:探讨宏基因捕获法(metagenomics capture,MetaCAP)二代测序技术在肺部感染病原学诊断中的临床应用价值。方法:选取2024年1—12月汕头市中心医院122例肺部感染患者。所有患者均通过支气管镜获取肺泡灌洗液标本,并行MetaCAP检测和传统病原学培养。比较两种检测方法在病原体阳性率、不同类型病原体的检出率以及混合感染检出率等方面的差异。结果:MetaCAP总体阳性检出率为89.3%(109/122),显著高于传统培养的39.3%(48/122)(P<0.05)。在各类病原体检测中,MetaCAP均展现出显著优势:细菌(80.3%vs.35.2%)、真菌(27.9%vs.4.9%)、结核分枝杆菌(8.2%vs.0%)及病毒(59.8%vs.0%),差异均具有统计学意义(P<0.05)。特别在混合感染检测方面,MetaCAP检出率66.1%(72/109)的三重及以上混合感染,而传统培养均为阴性。结论:MetaCAP二代测序技术可显著提高肺部感染病原体的检出率,尤其对病毒、结核分枝杆菌及混合感染的诊断具有独特优势。 展开更多
关键词 宏基因捕获法 二代测序 支气管肺泡灌洗液 肺部感染
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基于脑瘫患儿的外显子组测序:TruSeq与NimbleGen试剂盒的捕获性能比较
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作者 王妍龚 成业 +2 位作者 刘洋 许毅然 邢清和 《复旦学报(医学版)》 北大核心 2025年第6期868-876,共9页
目的比较TruSeq^(®)Exome与NimbleGen SeqCap EZ Human Exome两种外显子捕获试剂在脑瘫患儿中的捕获性能差异,为临床遗传学研究和诊断提供技术选择依据。方法纳入48例散发脑瘫患儿外周血样本,分别采用TruSeq(DNA探针)和NimbleGen(... 目的比较TruSeq^(®)Exome与NimbleGen SeqCap EZ Human Exome两种外显子捕获试剂在脑瘫患儿中的捕获性能差异,为临床遗传学研究和诊断提供技术选择依据。方法纳入48例散发脑瘫患儿外周血样本,分别采用TruSeq(DNA探针)和NimbleGen(RNA探针)构建外显子组文库,经Illumina HiSeq 2000平台测序。通过生物信息学分析评估比对率、目标区域覆盖度、变异检出一致性等指标,并基于脑瘫相关基因集(2293个基因)分析捕获性能的临床相关性,采用配对t检验进行统计学分析(显著性阈值α=0.05)。结果NimbleGen和TruSeq两种外显子组捕获试剂盒在基础数据质量(比对率、插入片段长度)和GC含量上差异无统计学意义。然而,在关键性能指标上呈现互补特征,NimbleGen在特定深度覆盖上表现更优(1×覆盖率,P=1.84×10^(-5);20×覆盖率,P=1.49×10^(-20));而TruSeq则展现出更高的Indel检测灵敏度(TruSeq vs.NimbleGen:11371±1689 vs.11274±1670,P=3.24×10^(-7))和罕见变异捕获能力(TruSeq vs.NimbleGen:3164±766 vs.3072±774,P=1.20×10^(-4)),并成功检出所有11个阳性致病变异(包括NimbleGen漏检的2例)。结论TruSeq凭借更优的变异检出率更适合临床诊断场景,而NimbleGen的覆盖稳定性可能有利于研究性项目。 展开更多
关键词 全外显子组测序(WES) 捕获 试剂盒 脑瘫
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黑药脱除废水中共存重金属Cu、Zn和Cd机理研究
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作者 韩敬瑞 王彦军 张旗 《化学工程》 北大核心 2025年第11期7-12,共6页
以黑药为重金属捕集剂,深入探究酸性工业废水中共存重金属Cu、Zn、Cd的脱除机理。通过对捕集剂及其螯合物进行FT-IR(傅里叶变换红外光谱)和XPS(X射线光电子能谱)结构表征,阐明了黑药活性基团二硫代磷酸根—P(=S)S—与重金属离子间的配... 以黑药为重金属捕集剂,深入探究酸性工业废水中共存重金属Cu、Zn、Cd的脱除机理。通过对捕集剂及其螯合物进行FT-IR(傅里叶变换红外光谱)和XPS(X射线光电子能谱)结构表征,阐明了黑药活性基团二硫代磷酸根—P(=S)S—与重金属离子间的配位机制。构建共存重金属体系,经实验测定获得重金属沉积顺序:Cu^(2+)>Cd^(2+)>Zn^(2+),进一步结合价键理论和Lewis软硬酸碱理论,从配合物轨道类型、电子接受能力及软硬酸碱方面进行沉积顺序机理解释。此外,负电螯合物对重金属离子的吸附电中和及多金属螯合物絮体增大,综合提升了药剂的沉淀性能,使共存重金属较单金属体系脱除效果更好。机理研究揭示了黑药捕集剂与废水中共存重金属间的作用规律,为进一步实际应用提供重要的理论依据。 展开更多
关键词 捕集剂 黑药 共存重金属 沉积顺序机理 螯合物
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