摘要
为了进一步提升高负荷接触稳定法(High-rate Contact-Stabilization, HiCS)的碳捕获能力,开展了污泥基生物炭强化HiCS工艺碳捕获及其对微生物群落影响的研究,并通过多种分析手段考察生物炭投加量对HiCS系统的COD捕获效率和NH+4-N去除率的影响。同时,考察生物炭投加量对污泥性质、胞外聚合物和微生物丰度变化的影响。结果显示,投加60 mg/L生物炭的碳捕获率最高为58%,较对照组提高15百分点。NH+4-N的去除效率在生物炭为80 mg/L时达到最高,为55.1%,较对照组提高了29.9百分点。此外,生物炭作为载体可以富集大量功能微生物,提高整体的生物量,通过对污泥粒径及扫描电子显微镜观察发现,投加生物炭后的污泥粒径有所减小且粒径分布较为集中,且污泥表面产生较多孔隙并更加粗糙,这使得污泥具有更强的捕获能力。通过测定Zeta电位发现,随着生物炭投加量的增加,污泥的Zeta电位的绝对值在6~8 mV内逐渐减小,这表明污泥絮凝能力得到了提升。污泥的ρ(MLVSS)/ρ(MLSS)也随着生物炭的投加而增加,表明投加生物炭增强了系统内的生物活性。生物炭的投加也促进了胞外聚合物的分泌,高通量测序结果显示,生物炭会提高吸附、降解污染物相关的优势菌门的丰度,如变形菌门和拟杆菌门。
To further improve the carbon capture capacity of the High-Load Contact Stabilization(HiCS)method,this study examined the reinforcement of carbon capture using sludge-based biochar and its effects on microbial communities.Various analytical tools were employed to investigate the impact of biochar dosing on Chemical Oxygen Demand(COD)capture efficiency and NH+4 N removal within the HiCS system.Additionally,the study explored how biochar dosing influenced changes in sludge properties,extracellular polymeric substances,and microbial abundance.The results revealed that the addition of 60 mg/L of biochar resulted in the highest carbon capture rate of 58%,representing a 15%improvement compared to the control group.Furthermore,the removal efficiency of NH+4 N peaked at 55.1%with a biochar dosing of 80 mg/L,which was 29.9%higher than the control group s performance.Furthermore,biochar acted as a carrier that enriched a diverse array of functional microorganisms.Observations of sludge particle size and Scanning Electron Microscopy revealed that the addition of biochar led to a reduction in sludge particle size,with a more concentrated distribution of particle sizes.Additionally,the surface of the sludge exhibited increased porosity and roughness,enhancing its capacity for carbon capture.Zeta potential measurements indicated that the absolute value of sludge zeta potential gradually decreased from 6 mV to 8 mV with increasing biochar dosage,suggesting an enhancement in the flocculation ability of the sludge.Additionally,the ratio ofρ(MLVSS)toρ(MLSS)also increased with the addition of biochar,indicating that the incorporation of biochar enhanced biological activity within the system.The addition of biochar also stimulated the secretion of Extracellular Polymers(EPS).Specifically,LB EPS levels in the system increased from 118.92 mg/g VSS to a range of 129.45 mg/g VSS to 157.47 mg/g VSS,while TB EPS rose from 204.98 mg/g VSS in the control group to between 223.66 mg/g VSS and 274.82 mg/g VSS.High-throughput sequencing results revealed that biochar enhanced the abundance of dominant phyla associated with pollutant adsorption and degradation,including Proteobacteria and Bacteroidetes.
作者
孙剑平
周佳迪
李绍峰
刘婉怡
SUN Jianping;ZHOU Jiadi;LI Shaofeng;LIU Wanyi(Municipal and Environmental Engineering College,Shenyang Jianzhu University,Shenyang 110168,China;Materials and Environmental Engineering College,Shenzhen Polytechnic University,Shenzhen 518055,Guangdong,China)
出处
《安全与环境学报》
北大核心
2025年第6期2361-2371,共11页
Journal of Safety and Environment
关键词
环境工程学
高负荷接触稳定法
碳捕获
生物炭
高通量测序
environmental engineering
high intensity contact stabilization
carbon capture
biochar
high-throughput sequence
