Resistant cultivar deployment is an effective method for cereal aphid management.Under greenhouse conditions,preliminary antibiosis resistance screening was conducted on 114 Ethiopian and 22 Chinese spring wheat acces...Resistant cultivar deployment is an effective method for cereal aphid management.Under greenhouse conditions,preliminary antibiosis resistance screening was conducted on 114 Ethiopian and 22 Chinese spring wheat accessions.After performing a bioassay to determine antibiosis resistance,aphid feeding behaviour and phenolic acid content analyses were performed on the aphid resistant wheat accessions by electrical penetration graph(EPG)and high performance liquid chromatography(HPLC),respectively.Among the wheat accessions,two high resistances,27moderate-resistances,and 35 low-resistances to Sitobion miscanthi were identified.The antibiosis resistance test showed prolonged pre-adult and pre-reproductive periods,shorter reproductive periods,lower fecundity,an intrinsic rate(rm)of increase,and a finite rate(λ)of increase of S.miscanthi on Lunxuan 145,Wane,Lunxuan 6,204511,Lunxuan 103and 5215 than those on the aphid-susceptible accession Beijing 837.The changes for the parameters of aphid feeding behaviour,including spending a longer time in the penetration and phloem salivation phases and less time in the phloem sap-feeding phase on the resistant wheat accessions,indicated that the aphid resistance may occur during the phloem phase and may be due to difficulties in the mechanical probing of the mesophyll cells.Additionally,the HPLC analysis showed higher contents of:1)ferulic acid in Lunxuan 145,Lunxuan 103 and Lunxuan 6;2)p-coumaric acid in Lunxuan145;3)vanillic acid in Lunxuan 145,Wane and Lunxuan 6;4)syringic acid in Lunxuan 103;and 5)caffeic acid in 5215.The contents of some phenolic acids within wheat leaves,such as p-courmaric acid and vanillic acid showed significant positive correlation with the duration of aphid development,but negative correlation with the aphid fecundity.The concentrations of these acids may be the causes of antibiosis resistance to S.miscanthi.The identification of grain aphid-resistant wheat accessions in our study will be helpful in future breeding program for pest control.展开更多
The wheat aphid, Sitobion miscanthi, is one of the most destructive pests of wheat plants in the temperate regions of China. Little is known about the genetic structure evolution of the different geographic population...The wheat aphid, Sitobion miscanthi, is one of the most destructive pests of wheat plants in the temperate regions of China. Little is known about the genetic structure evolution of the different geographic populations of S. miscanthi with its migration. In this study, we investigated the population genetic structure and demographic history of S. miscanthi by analysing 18 geographical populations across China using one mitochondrial gene, COI;one nuclear gene, EF-1α;and two endosymbiont Buchnera genes, gnd and trpA. Analysis of data from the various groups showed high haplotype diversity and low nucleotide variation. SAMOVA analysis did not find a correlation between genetic distance and geographic distance. However, areas with high population diversity exhibited high haplotype diversity. Therefore, we speculate that there are two main natural migration pathways of S. miscanthi in China. One is from Yunnan to the Sichuan Basin, and the other is from Wuhan, Xinyang and Jiaodong Peninsula areas to the northwest. Based on this hypothesis, we inferred that these aphid populations appear first in the southwestern and southern regions and spread to the north with the help of the southeastern and southwestern monsoons, which occur in spring and summer. In autumn, the aphids spread southward with the northeastern and northwestern monsoons.展开更多
基金supported by the National Natural Science Foundation of China(31871979 and 31901881)the National Key R&D Program of China(2017YFD0201700,2017YFD0200900 and 2016YFD0300700)the China’s Donation to the Centre Agriculture Bioscience International(CABI)Development Fund。
文摘Resistant cultivar deployment is an effective method for cereal aphid management.Under greenhouse conditions,preliminary antibiosis resistance screening was conducted on 114 Ethiopian and 22 Chinese spring wheat accessions.After performing a bioassay to determine antibiosis resistance,aphid feeding behaviour and phenolic acid content analyses were performed on the aphid resistant wheat accessions by electrical penetration graph(EPG)and high performance liquid chromatography(HPLC),respectively.Among the wheat accessions,two high resistances,27moderate-resistances,and 35 low-resistances to Sitobion miscanthi were identified.The antibiosis resistance test showed prolonged pre-adult and pre-reproductive periods,shorter reproductive periods,lower fecundity,an intrinsic rate(rm)of increase,and a finite rate(λ)of increase of S.miscanthi on Lunxuan 145,Wane,Lunxuan 6,204511,Lunxuan 103and 5215 than those on the aphid-susceptible accession Beijing 837.The changes for the parameters of aphid feeding behaviour,including spending a longer time in the penetration and phloem salivation phases and less time in the phloem sap-feeding phase on the resistant wheat accessions,indicated that the aphid resistance may occur during the phloem phase and may be due to difficulties in the mechanical probing of the mesophyll cells.Additionally,the HPLC analysis showed higher contents of:1)ferulic acid in Lunxuan 145,Lunxuan 103 and Lunxuan 6;2)p-coumaric acid in Lunxuan145;3)vanillic acid in Lunxuan 145,Wane and Lunxuan 6;4)syringic acid in Lunxuan 103;and 5)caffeic acid in 5215.The contents of some phenolic acids within wheat leaves,such as p-courmaric acid and vanillic acid showed significant positive correlation with the duration of aphid development,but negative correlation with the aphid fecundity.The concentrations of these acids may be the causes of antibiosis resistance to S.miscanthi.The identification of grain aphid-resistant wheat accessions in our study will be helpful in future breeding program for pest control.
基金funded by the National Natural Science Foundation of China(31871979,32001900 and 31901881)the National Key R&D Program in China(2017YFD0201700,2017YFD0200900 and 2016YFD0300700)the China Agriculture Research System of MOF and MARA(CARS-22)。
文摘The wheat aphid, Sitobion miscanthi, is one of the most destructive pests of wheat plants in the temperate regions of China. Little is known about the genetic structure evolution of the different geographic populations of S. miscanthi with its migration. In this study, we investigated the population genetic structure and demographic history of S. miscanthi by analysing 18 geographical populations across China using one mitochondrial gene, COI;one nuclear gene, EF-1α;and two endosymbiont Buchnera genes, gnd and trpA. Analysis of data from the various groups showed high haplotype diversity and low nucleotide variation. SAMOVA analysis did not find a correlation between genetic distance and geographic distance. However, areas with high population diversity exhibited high haplotype diversity. Therefore, we speculate that there are two main natural migration pathways of S. miscanthi in China. One is from Yunnan to the Sichuan Basin, and the other is from Wuhan, Xinyang and Jiaodong Peninsula areas to the northwest. Based on this hypothesis, we inferred that these aphid populations appear first in the southwestern and southern regions and spread to the north with the help of the southeastern and southwestern monsoons, which occur in spring and summer. In autumn, the aphids spread southward with the northeastern and northwestern monsoons.
