By using an LKB-2277 Bioactivity Monitor, the effect ofporphyrin-cholesterol esters on cancer cells RL-60 were examined at 25℃.There is a dirrerence in the shape of the thermogenesls curve samong different kinds or ...By using an LKB-2277 Bioactivity Monitor, the effect ofporphyrin-cholesterol esters on cancer cells RL-60 were examined at 25℃.There is a dirrerence in the shape of the thermogenesls curve samong different kinds or Porphyrln-cholesterol esters,and the death rate or HL-60 cells is also different. The results show that porphyrin-chsterol esters can powerfully inhibit their metabolism, the inhibitory sequence is Ⅱ >I >Ⅲ,but the inhibitory way of each ester is different.展开更多
The complex of samaric chloride lower hydrate with diethylammoniumdiethyldithiocarbamate (D-DDC) was synthesized conveniently in absolute alcohol and dry N_2atmosphere. The title complex was identified as Et_2NH_2[Sm(...The complex of samaric chloride lower hydrate with diethylammoniumdiethyldithiocarbamate (D-DDC) was synthesized conveniently in absolute alcohol and dry N_2atmosphere. The title complex was identified as Et_2NH_2[Sm(S_2CNEt_2)_4] by chemical and elementalanalyses, the bonding characteristics of which was characterized by IR. The enthalpies of solutionof samaric chloride hydrate and D-DDC in absolute alcohol at 298.15 K and the enthalpies change ofliquid-phase reaction of formation for Et_2NH_2[Sm(S_2CNEt_2)_4] at different temperatures weredetermined by mi-crocalorimetry. On the basis of experimental and calculated results, threethermodynamic parameters (the activation enthalpy, the activation entropy, and the activation freeenergy), the rate constant, and three kinetic parameters (the apparent activation energy, thepre-exponential constant, and the reaction order) of liquid phase reaction of formation wereobtained. The enthalpy change of the solid-phase title reaction at 298.15 K was calculated by athermochemical cycle.展开更多
The complex of neodymium chloride lower hydrate with diethylammonium diethyldithiocarbamate (D-DDC) was synthesized conveniently in absolute alcohol and dry N_2 atmosphere. The title complex was identified as Et_2NH_2...The complex of neodymium chloride lower hydrate with diethylammonium diethyldithiocarbamate (D-DDC) was synthesized conveniently in absolute alcohol and dry N_2 atmosphere. The title complex was identified as Et_2NH_2[Nd(S_2CNEt_2)_4] by chemical and elemental analyses and the bonding characteristics of which was characterized by IR. The enthalpies of solution of neodymium chloride hydrate and D-DDC in absolute alcohol at 298.15 K and the enthalpies change of liquid-phase reaction of formation for Et_2NH_2[Nd (S_2CNEt_2)_4] at different temperatures were determined by microcalorimetry. On the basis of experimental and calculated results, three thermodynamic parameters (the activation enthalpy, the activation entropy and the activation free energy), the rate constant and three kinetic parameters (the apparent activation energy, the pre-exponential constant and the reaction order) of liquid-phase reaction of formation were obtained. The enthalpy change of the solid-phase title reaction at 298.15 K was calculated by a thermochemical cycle.展开更多
It has been found that the two-phase reactions of aqueous HCl, HOAc or H_3PO_4 with primary amine N_ 1923 in chloroform are oscillating reactions. Their power-time curves were measured by the titration microcalorimet...It has been found that the two-phase reactions of aqueous HCl, HOAc or H_3PO_4 with primary amine N_ 1923 in chloroform are oscillating reactions. Their power-time curves were measured by the titration microcalorimetric method, and the induction period (t_ in). The first oscillating period (t_ p.1) and the second oscillating period (t_ p.2) were determined. The apparent activating parameters and the orders of the oscillating systems were calculated and the following relationships were established: for the oscillating system of hydrochloric acid t_ in∝c 0.147_ HClexp(1.35×10 3T), t_ p.1∝c 0.241_ HCl· exp(4.33×10 3T), t_ p.2∝c 0.290_ HClexp(5.59×10 3T); for the oscillating system of acetic acid, t_ in∝c 0.883_ HOAcexp(2.32×10 3T), t_ p.1∝c 0.399_ HOAc· exp(4.50×10 3T), t_ p.2∝c 0.301_ HOAcexp(5.88×10 3T); for the oscillating system of phosphoric acid, t_ in∝c 1.14_ H_3PO_4exp(7.70×10 4T), t_ p.1∝c 1.42_ H_3PO_4exp(1.14×10 4T), t_ p.2∝c 1.47_ H_3PO_4exp(1.27×10 4T).展开更多
The microcalorimetric method was used to study the antibacterial activity of two newly synthesized Schiff base compounds （H2L3＇ and H2L3） on Escherichia coli, trying to obtain the action on both of multiplying bact...The microcalorimetric method was used to study the antibacterial activity of two newly synthesized Schiff base compounds （H2L3＇ and H2L3） on Escherichia coli, trying to obtain the action on both of multiplying bacteria and non-multiplying bacteria at one experiment. The metabolic power-time curves of the bacteria treated with the compounds were obtained, and the thermokinetic parameters were analyzed, from which the antibacterial activities of these compounds were evaluated. The results showed that both of the two compounds have good activity on aerobic multiplying metabolism of E. coli, with the value of ICso 75.8 and 168.8 mg/L respectively, but have not effective action on fermentation metabolism of E. coli. The action of the compounds on the non-multiplying metabolism was investigated by taking the heat output of E. coli in the stationary phase as the guideline of the activity. The value of MSCso （minimum stationary-cidal concentration 50） of them is 118 and 187.5 mg/L, respectively. So, H2L^3 has stronger antibacterial action on E. coli than H2L^3 either for multiplying bacteria or non-multiplying bacteria, and their activity on the aerobic multiplying bacteria of E. coil is mainly shown. It does strongly suggest that the calorimetric method should play an important role in the fight against the drug-resistant bacteria.展开更多
文摘By using an LKB-2277 Bioactivity Monitor, the effect ofporphyrin-cholesterol esters on cancer cells RL-60 were examined at 25℃.There is a dirrerence in the shape of the thermogenesls curve samong different kinds or Porphyrln-cholesterol esters,and the death rate or HL-60 cells is also different. The results show that porphyrin-chsterol esters can powerfully inhibit their metabolism, the inhibitory sequence is Ⅱ >I >Ⅲ,but the inhibitory way of each ester is different.
基金This work is financially supported by the National Natural Science Foundation of China (No. 20171036) and the Education Department of Shaanxi Province (No. 01JK229)
文摘The complex of samaric chloride lower hydrate with diethylammoniumdiethyldithiocarbamate (D-DDC) was synthesized conveniently in absolute alcohol and dry N_2atmosphere. The title complex was identified as Et_2NH_2[Sm(S_2CNEt_2)_4] by chemical and elementalanalyses, the bonding characteristics of which was characterized by IR. The enthalpies of solutionof samaric chloride hydrate and D-DDC in absolute alcohol at 298.15 K and the enthalpies change ofliquid-phase reaction of formation for Et_2NH_2[Sm(S_2CNEt_2)_4] at different temperatures weredetermined by mi-crocalorimetry. On the basis of experimental and calculated results, threethermodynamic parameters (the activation enthalpy, the activation entropy, and the activation freeenergy), the rate constant, and three kinetic parameters (the apparent activation energy, thepre-exponential constant, and the reaction order) of liquid phase reaction of formation wereobtained. The enthalpy change of the solid-phase title reaction at 298.15 K was calculated by athermochemical cycle.
文摘The complex of neodymium chloride lower hydrate with diethylammonium diethyldithiocarbamate (D-DDC) was synthesized conveniently in absolute alcohol and dry N_2 atmosphere. The title complex was identified as Et_2NH_2[Nd(S_2CNEt_2)_4] by chemical and elemental analyses and the bonding characteristics of which was characterized by IR. The enthalpies of solution of neodymium chloride hydrate and D-DDC in absolute alcohol at 298.15 K and the enthalpies change of liquid-phase reaction of formation for Et_2NH_2[Nd (S_2CNEt_2)_4] at different temperatures were determined by microcalorimetry. On the basis of experimental and calculated results, three thermodynamic parameters (the activation enthalpy, the activation entropy and the activation free energy), the rate constant and three kinetic parameters (the apparent activation energy, the pre-exponential constant and the reaction order) of liquid-phase reaction of formation were obtained. The enthalpy change of the solid-phase title reaction at 298.15 K was calculated by a thermochemical cycle.
基金ProjectsupportedbytheNaturalScienceFoundationofShandongProvince (No .Y2 0 0 0B0 3)
文摘It has been found that the two-phase reactions of aqueous HCl, HOAc or H_3PO_4 with primary amine N_ 1923 in chloroform are oscillating reactions. Their power-time curves were measured by the titration microcalorimetric method, and the induction period (t_ in). The first oscillating period (t_ p.1) and the second oscillating period (t_ p.2) were determined. The apparent activating parameters and the orders of the oscillating systems were calculated and the following relationships were established: for the oscillating system of hydrochloric acid t_ in∝c 0.147_ HClexp(1.35×10 3T), t_ p.1∝c 0.241_ HCl· exp(4.33×10 3T), t_ p.2∝c 0.290_ HClexp(5.59×10 3T); for the oscillating system of acetic acid, t_ in∝c 0.883_ HOAcexp(2.32×10 3T), t_ p.1∝c 0.399_ HOAc· exp(4.50×10 3T), t_ p.2∝c 0.301_ HOAcexp(5.88×10 3T); for the oscillating system of phosphoric acid, t_ in∝c 1.14_ H_3PO_4exp(7.70×10 4T), t_ p.1∝c 1.42_ H_3PO_4exp(1.14×10 4T), t_ p.2∝c 1.47_ H_3PO_4exp(1.27×10 4T).
基金Project supported by the National Natural Science Foundation of China （Nos. 30570015 and 20373051）, the Teaching and Research Award Program for 0utstanding Young Professors of High Education Institutes, Ministry of Education, China （2001）.
文摘The microcalorimetric method was used to study the antibacterial activity of two newly synthesized Schiff base compounds （H2L3＇ and H2L3） on Escherichia coli, trying to obtain the action on both of multiplying bacteria and non-multiplying bacteria at one experiment. The metabolic power-time curves of the bacteria treated with the compounds were obtained, and the thermokinetic parameters were analyzed, from which the antibacterial activities of these compounds were evaluated. The results showed that both of the two compounds have good activity on aerobic multiplying metabolism of E. coli, with the value of ICso 75.8 and 168.8 mg/L respectively, but have not effective action on fermentation metabolism of E. coli. The action of the compounds on the non-multiplying metabolism was investigated by taking the heat output of E. coli in the stationary phase as the guideline of the activity. The value of MSCso （minimum stationary-cidal concentration 50） of them is 118 and 187.5 mg/L, respectively. So, H2L^3 has stronger antibacterial action on E. coli than H2L^3 either for multiplying bacteria or non-multiplying bacteria, and their activity on the aerobic multiplying bacteria of E. coil is mainly shown. It does strongly suggest that the calorimetric method should play an important role in the fight against the drug-resistant bacteria.
