Microorganisms constitute an essential component in the indoor environment,which is closely related to hu-man health.However,there is limited evidence regarding the associations between indoor airborne microbiome and ...Microorganisms constitute an essential component in the indoor environment,which is closely related to hu-man health.However,there is limited evidence regarding the associations between indoor airborne microbiome and systemic inflammation,as well as whether this association is modified by indoor particulate matter and the underlying mechanisms.In this prospective repeated-measure study among 66 participants,indoor airborne mi-crobiome was characterized using amplicon sequencing and qPCR.Indoor fine particulate matter(PM_(2.5))and inhalable particulate matter(PM10)were measured.Systemic inflammatory biomarkers were assessed,including white blood cell(WBC),neutrophil(NEUT),monocyte,eosinophil counts,and their proportions.Targeted serum amino acid metabolomics were conducted to explore the underlying mechanisms.Linear mixed-effect models re-vealed that bacterial and fungal Simpson diversity were significantly associated with decreased WBC and NEUT.For example,for each interquartile range increase in the bacterial Simpson diversity,WBC and NEUT changed by-4.53%(95%CI:-8.25%,-0.66%)and-5.95%(95%CI:-11.3%,-0.27%),respectively.Notably,increased inflammatory risks of airborne microbial exposure were observed when indoor PM_(2.5) and PM10 levels were below the WHO air quality guidelines.Mediation analyses indicated that dopamine metabolism partially mediated the anti-inflammatory effects of fungal diversity exposure.Overall,our study indicated protection from a diverse indoor microbial environment on cardiovascular health and proposed an underlying mechanism through amino acid metabolism.Additionally,health risks associated with microbial exposure deserve more attention in con-texts of low indoor particulate matter pollution.Further research is necessary to fully disentangle the complex relationships between indoor microbiome,air pollutants,and human health.展开更多
The gut microbiome comprises a vast community of microbes inhabiting the human alimentary canal,playing a crucial role in various physiological functions.These microbes generally live in harmony with the host;however,...The gut microbiome comprises a vast community of microbes inhabiting the human alimentary canal,playing a crucial role in various physiological functions.These microbes generally live in harmony with the host;however,when dysbiosis occurs,it can contribute to the pathogenesis of diseases,including osteoporosis.Osteoporosis,a systemic skeletal disease characterized by reduced bone mass and increased fracture risk,has attracted significant research attention concerning the role of gut microbes in its development.Advances in molecular biology have highlighted the influence of gut microbiota on osteoporosis through mechanisms involving immunoregulation,modulation of the gut-brain axis,and regulation of the intestinal barrier and nutrient absorption.These microbes can enhance bone mass by inhibiting osteoclast differentiation,inducing apoptosis,reducing bone resorption,and promoting osteoblast proliferation and maturation.Despite these promising findings,the therapeutic effectiveness of targeting gut microbes in osteoporosis requires further investigation.Notably,gut microbiota has been increasingly studied for their potential in early diagnosis,intervention,and as an adjunct therapy for osteoporosis,suggesting a growing utility in improving bone health.Further research is essential to fully elucidate the therapeutic potential and clinical application of gut microbiome modulation in the management of osteoporosis.展开更多
BACKGROUND The gastric microbiome is closely associated with gastric cancer,and single-region 16S rRNA sequencing has limitations in analyzing its characteristics,necessitating the search for a better sequencing metho...BACKGROUND The gastric microbiome is closely associated with gastric cancer,and single-region 16S rRNA sequencing has limitations in analyzing its characteristics,necessitating the search for a better sequencing method.AIM To evaluate the effectiveness of multi-region 16S rRNA gene sequencing in studying the microbiome of gastric cancer tissues.METHODS Patients with gastric cancer(n=118)who underwent surgery at Liyang People's Hospital from January 2022 to December 2024 were enrolled.Fifty-nine paraffinembedded and 59 fresh tissue samples were obtained.The ZymoBIOMICSTM microbial community standard and Escherichia coli ATCC 25922 were used as positive controls.Multi-region and single-region 16S rRNA gene sequencing were performed.Species identification,detection rates at varying microbial abundances,operational taxonomic unit(OTU)counts,and alpha diversity indices in gastric cancer tissues were compared between the two methods.RESULTS Multi-region 16S rRNA sequencing identified more species(eight species and eight genera)in the positive controls compared with single-region sequencing(one species and six genera).Detection rates at concentrations of 103,102,and 10 CFU/mg were significantly higher using multi-region sequencing(P<0.05).Multi-region sequencing also revealed significantly higher OTU counts and alpha diversity indices(Shannon,Simpson,and Chao1)in gastric cancer tissues(P<0.05).CONCLUSION Compared with single-region sequencing,multi-region 16S rRNA gene sequencing demonstrates superior species resolution and detection sensitivity,providing a more comprehensive profile of microbial diversity in gastric cancer tissues.展开更多
Background Dietary protein level and amino acid(AA)balance are crucial determinants of animal health and productivity.Supplementing rumen-protected AAs in low-protein diets was considered as an efficient strategy to i...Background Dietary protein level and amino acid(AA)balance are crucial determinants of animal health and productivity.Supplementing rumen-protected AAs in low-protein diets was considered as an efficient strategy to improve the growth performance of ruminants.The colon serves as a crucial conduit for nutrient metabolism during rumen-protected methionine(RPMet)and rumen-protected lysine(RPLys)supplementation,however,it has been challenging to clarify which specific microbiota and their metabolites play a pivotal role in this process.Here,we applied metagenomic and metabolomic approaches to compare the characteristic microbiome and metabolic strategies in the colon of lambs fed a control diet(CON),a low-protein diet(LP)or a LP diet supplemented with RPMet and RPLys(LR).Results The LP treatment decreased the average daily weight gain(ADG)in lambs,while the LR treatment tended to elicit a remission in ADG.The butyrate molar concentration was greater(P<0.05),while acetate molar concentration(P<0.05)was lower for lambs fed the LP and LR diets compared to those fed the CON diet.Moreover,the LP treatment remarkably decreased total AA concentration(P<0.05),while LR treatment showed an improvement in the concentrations of methionine,lysine,leucine,glutamate,and tryptophan.Metagenomic insights proved that the microbial metabolic potentials referring to biosynthesis of volatile fatty acids(VFAs)and AAs in the colon were remarkably altered by three dietary treatments.Metagenomic binning identified distinct microbial markers for the CON group(Alistipes spp.,Phocaeicola spp.,and Ruminococcus spp.),LP group(Fibrobacter spp.,Prevotella spp.,Ruminococcus spp.,and Escherichia coli),and LR group(Akkermansia muciniphila and RUG099 spp.).Conclusions Our findings suggest that RPMet and RPLys supplementation to the low-protein diet could enhance the microbial biosynthesis of butyrate and amino acids,enriche the beneficial bacteria in the colon,and thereby improve the growth performance of lambs.展开更多
The intricate interplay between natural compounds like curcumin and the gut microbiome has gained significant attention in recent years due to their potential therapeutic implications in various health conditions.Curc...The intricate interplay between natural compounds like curcumin and the gut microbiome has gained significant attention in recent years due to their potential therapeutic implications in various health conditions.Curcumin,a polyphenolic compound derived from turmeric,exhibits diverse pharmacological properties,including anti-inflammatory,antioxidant,and anticancer effects.Understanding how curcumin modulates gut microbiota composition and function is crucial for elucidating its therapeutic mechanisms.This review examines the current literature on the interactions between curcumin and the gut microbiome.A systematic search of relevant databases was conducted to identify studies investigating the effects of curcumin on gut microbial diversity and abundance.Key findings from studies exploring curcumin's efficacy in neurological disorders,gastrointestinal diseases,and metabolic dysfunction are synthesized and discussed.Studies have demonstrated that curcumin supplementation can modulate gut microbiota composition and function,leading to beneficial effects on gut health and homeostasis.Mechanisms underlying curcumin's therapeutic effects include immune modulation,neuroprotection,and inflammation regulation.However,challenges such as poor bioavailability and safety concerns remain significant hurdles to overcome.The interactions between curcumin and the gut microbiome hold promise for therapeutic interventions in a diverse range of health conditions.Further research is needed to optimize curcumin formulations,improve bioavailability,and address safety concerns.展开更多
This study investigates the diversity of gut microbiota in Metaphire peguana,an earthworm species commonly found in agricultural areas of Thailand.Earthworms play a critical role in soil ecosystems by supporting nutri...This study investigates the diversity of gut microbiota in Metaphire peguana,an earthworm species commonly found in agricultural areas of Thailand.Earthworms play a critical role in soil ecosystems by supporting nutrient cycling and breaking down organic matter.Understanding the microbial diversity in their gut is essential for exploring their ecological contributions.Using Next Generation Sequencing(NGS),we analyzed the mycobiome in the gut of M.peguana.Our findings revealed a high diversity of fungal species,primarily belonging to two major phyla:Ascomycota and Basidiomycota.Ascomycota was the most abundant phylum,comprising 40.1% of the total fungal species identified.A total of 33 distinct fungal species were identified,which underscores the richness of microbial life within the earthworm gut.This study successfully created the first genetic database of the microbial community in M.peguana,providing a foundation for future research in agricultural applications.The microbial species identified,particularly siderophoreproducing fungi,could have significant implications for improving soil fertility and promoting sustainable agricultural practices.The use of NGS technology has enabled comprehensive profiling of microbial communities,allowing for precise identification of fungi that may play essential roles in soil health.Furthermore,the study paves the way for future studies on the potential applications of earthworm gut microbiomes in biotechnology,especially in enhancing soil nutrient availability and plant growth.The findings of this research contribute to the broader understanding of the ecological roles of earthworms and their microbiomes in soil ecosystems.展开更多
The environments of tropical and subtropical coral reef regions(CRR)differ from each other;however,it is not known if these environmental differences influence coral polyp and skeleton microbiome composition.In this s...The environments of tropical and subtropical coral reef regions(CRR)differ from each other;however,it is not known if these environmental differences influence coral polyp and skeleton microbiome composition.In this study,Coelastrea palauensis corals were collected from tropical and subtropical CRR in the South China Sea,and bacterial,archaeal,and fungal communities in polyps and skeletons were analyzed.Results showed that the microbial diversity and composition of C.palauensis significantly differed between the polyps and skeletons,and between the tropical and subtropical CRR.Regarding bacteria associated with corals,C.palauensis was mainly associated with bacteria closely related to the nitrogen cycle in the subtropical CRR.The relative abundances of Terasakiellaceae and Chlorobium in both coral polyps and skeletons in the subtropical CRR were higher than those in the tropical CRR.In the tropical CRR,C.palauensis was mainly associated with opportunistic pathogenic bacteria.The relative abundances of Tenacibaculum and Vibrio in coral polyps and skeletons in the tropical CRR were higher than those in the subtropical CRR.Regarding archaea associated with corals,polyps and skeletons of C.palauensis in both tropical and subtropical reef areas were dominated by n_Woesearchaeales,and the relative abundance of n_Woesearchaeales in skeletons is significantly higher than that in polyps.In addition,the relative abundances of n_Woesearchaeales in polyps and skeletons in the subtropical CRR were significantly higher than those in the tropical CRR.Regarding fungi associated with corals,Ascomycota was dominant in polyps and skeletons in the subtropical CRR,while Sordariomycetes,Periconia,Cladosporium,and Aspergillus were dominant in polyps and skeletons in the tropical CRR.Besides,the diversity differences of coral-associated microorganisms were related to environmental factors such as nutrients and temperature that may affect the survival of coral-associated microorganisms.These results implied that corals may adjust the composition of microorganisms,conducive the coral holobiont to better adapting the environment.Our research will be beneficial in understanding the differences and adaptations of coral polyp and skeletal microbiome.展开更多
The community of microorganisms that colonize certain areas of the human body is called microbiota.Microorganisms such as bacteria,fungi and viruses make up the microbiota.The sum of the genomes of these microorganism...The community of microorganisms that colonize certain areas of the human body is called microbiota.Microorganisms such as bacteria,fungi and viruses make up the microbiota.The sum of the genomes of these microorganisms and microorganisms refers to the microbiome.It has been shown that microbiota has important effects such as protecting the organ from pathogens,contributing to metabolic functions(such as vitamin synthesis,carbohydrate digestion)and providing immunoregulation.Dysbiosis refers to compositional and functional changes in the microbiota.At the beginning of the 21st century,numerous studies have investigated the human microbiota and its imbalance in relation to various diseases and found that dysbiosis is associated with many diseases.The aim of this minireview article is to provide brief information about dysbiosis and its care and to raise awareness.展开更多
BACKGROUND The gut microbiome is integral to human health,with emerging research underscoring its potential impact on ocular health through the gut-eye axis.Various ocular disorders,such as dry eye syndrome,retinal va...BACKGROUND The gut microbiome is integral to human health,with emerging research underscoring its potential impact on ocular health through the gut-eye axis.Various ocular disorders,such as dry eye syndrome,retinal vascular diseases,macular degeneration,and glaucoma,may be influenced by gut dysbiosis,which could significantly contribute to their development and progression.AIM To evaluate the influence of the gut microbiome on the pathogenesis and progression of various ocular diseases.METHODS An extensive search of the scientific literature was undertaken by adhering to Preferred Reporting Items for Systematic Reviews&Meta-Analyses standards,using PubMed(MEDLINE),Scopus,EMBASE,and the Cochrane Library as sources to locate studies addressing the relationship between the gut microbiome and human health.To capture all relevant publications,search terms were systematically applied across these major databases,without limiting the search by language or publication date.Inclusion criteria covered randomized controlled trials,non-randomized controlled trial,prospective studies,cross-sectional studies,and case-control studies.Out of the 3077 articles,36 full texts were included in the review.RESULTS Ocular health appears to be shaped by the gut microbial community through mechanisms such as immune regulation,preservation of the blood–retinal barrier,and the generation of protective metabolites.Disturbances in this microbial balance can provoke measurable alterations in host immunity,providing a plausible immunopathogenic pathway that connects intestinal dysbiosis with eye disease.Both laboratory models and early human data suggest that targeted interventions,including prebiotics,probiotics,synbiotics,and faecal microbiota transfer,hold therapeutic potential.CONCLUSION The gut–eye relationship reflects a multifaceted interaction in which the intestinal microbiome contributes to ocular health through complex biological pathways.Integrating microbiome assessments into diagnostic methods can revolutionize disease management through early detection and targeted interventions.Further,randomised controlled clinical trials are necessary for ocular diseases to prove causal relationships.展开更多
Menopause is characterized by the cessation of menstruation and a decline in reproductive function,which is an intrinsic component of the aging process.However,it has been a frequently overlooked field of women’s hea...Menopause is characterized by the cessation of menstruation and a decline in reproductive function,which is an intrinsic component of the aging process.However,it has been a frequently overlooked field of women’s health.The oral and gut microbiota,constituting the largest ecosystem within the human body,are important for maintaining human health and notably contribute to the healthy aging of menopausal women.Therefore,a comprehensive review elucidating the impact of the gut and oral microbiota on menopause for healthy aging is of paramount importance.This paper presents the current understanding of the microbiome during menopause,with a particular focus on alterations in the oral and gut microbiota.Our study elucidates the complex interplay between the microbiome and sex hormone levels,explores microbial crosstalk dynamics,and investigates the associations between the microbiome and diseases linked to menopause.Additionally,this review explores the potential of microbiome-targeting therapies for managing menopause-related diseases.Given that menopause can last for approximately 30 years,gaining insights into how the microbiome and menopause interact could pave the way for innovative interventions,which may result in symptomatic relief from menopause and an increase in quality of life in women.展开更多
Phyllosphere microbiome plays an irreplaceable role in maintaining plant health under stress,but its structure and functions in heavy metal-hyperaccumulating plants remain elusive.Here,the phyllosphere microbiome,inha...Phyllosphere microbiome plays an irreplaceable role in maintaining plant health under stress,but its structure and functions in heavy metal-hyperaccumulating plants remain elusive.Here,the phyllosphere microbiome,inhabiting hyperaccumulating(HE)and non-hyperaccumulating ecotype(NHE)of Sedum alfredii grown in soils with varying heavy metal concentration,was characterized.Compared with NHE,the microbial communityα-diversity was greater in HE.Core phyllosphere taxa with high relative abundance(>10%),including Streptomyces and Nocardia(bacteria),Cladosporium and Acremonium(fungi),were significantly related to cadmium(Cd)and zinc(Zn)concentration and biomass of host plants.Moreover,microbial co-occurrence networks in HE exhibited greater complexity than those in NHE.Additionally,proportions of positive associations in HE bacterial networks increased with the rising heavy metal concentration,indicating a higher resistance of HE phyllosphere microbiome to heavy metal stress.Furthermore,in contrast to NHE,microbial community functions,primarily involved in heavy metal stress resistance,were more abundant in HE,in which microbiome assisted hosts to resist heavy metal stress better.Collectively,this study indicated that phyllosphere microbiome of the hyperaccumulator played an indispensable role in assisting hosts to resist heavy metal stress,and provided new insights into phyllosphere microbial application potential in phytoremediation.展开更多
Atrial fibrillation(AF)is a growing global health burden,with a prevalence of over 52.55 million cases.Rising disability-adjusted life-years,increasing age,and disparities in care have contributed to the worsening sev...Atrial fibrillation(AF)is a growing global health burden,with a prevalence of over 52.55 million cases.Rising disability-adjusted life-years,increasing age,and disparities in care have contributed to the worsening severity and mortality of AF.Modifiable risk factors,such as hypertension,obesity,and diabetes mellitus,are associated with alterations in gut microbiota,making the gut-heart axis a potential therapeutic target.Gut dysbiosis influences AF pathogenesis through inflam-mation,metabolic disruption,and autonomic dysfunction.Key mechanisms include gut barrier dysfunction,short-chain fatty acid(SCFA)depletion,lipopoly-saccharides(LPS)-induced inflammation,and ferroptosis-mediated atrial remodeling.Trimethylamine N-oxide,bile acids,and tryptophan metabolites contribute to arrhythmogenic remodeling.Emerging evidence suggests that dietary interventions,including prebiotics and probiotics,as well as gut surveillance,may help mitigate AF progression.Clinical implications of gut modulation in AF include person-alized dietary strategies,microbiome assessment through metagenomic sequencing,and targeted interventions such as SCFA-based therapies and ferroptosis inhibition.Metabolite surveillance,including LPS and indoxyl sulfate monitoring,may influence the effectiveness of anticoagulant and antiarrhythmic therapy.Despite growing mechanistic evidence linking gut dysbiosis to AF,clinical applications remain unexplored.This review summarizes the current understanding of the gut microbiome's role in AF.展开更多
The gut microbiome,a complex ecosystem of microorganisms,has a significant role in modulating pain,particularly within orthopaedic conditions.Its impact on immune and neurological functions is underscored by the gut-b...The gut microbiome,a complex ecosystem of microorganisms,has a significant role in modulating pain,particularly within orthopaedic conditions.Its impact on immune and neurological functions is underscored by the gut-brain axis,which influences inflammation,pain perception,and systemic immune responses.This integrative review examines current research on how gut dysbiosis is associated with various pain pathways,notably nociceptive and neuroinflammatory mechanisms linked to central sensitization.We highlight advancements in meta-omics technologies,such as metagenomics and metaproteomics,which deepen our understanding of microbiome-host interactions and their implications in pain.Recent studies emphasize that gut-derived short-chain fatty acids and microbial metabolites play roles in modulating neuroinflammation and nociception,contributing to pain management.Probiotics,prebiotics,synbiotics,and faecal microbiome transplants are explored as potential therapeutic strategies to alleviate pain through gut microbiome modulation,offering an adjunct or alternative to opioids.However,variability in individual microbiomes poses challenges to standardizing these treatments,necessitating further rigorous clinical trials.A multidisciplinary approach combining microbiology,immunology,neurology,and orthopaedics is essential to develop innovative,personalized pain management strategies rooted in gut health,with potential to transform orthopaedic pain care.展开更多
Brief description Climate change represents an unparalleled existential threat to humanity in the twenty-first century,demanding urgent and sustained global attention.Among the vast array of actors shaping Earth’s cl...Brief description Climate change represents an unparalleled existential threat to humanity in the twenty-first century,demanding urgent and sustained global attention.Among the vast array of actors shaping Earth’s climate system,microorganisms occupy a uniquely significant position.As the planet’s most abundant and diverse life forms,they not only respond sensitively to climatic change but also exert profound influence upon them.Microbes-comprising viruses,bacteria,archaea,fungi,algae,and protozoa-pervade from terrestrial soils and urban infrastructures to atmospheric layers,subterranean environments,and aquatic ecosystems.By virtue of their staggering abundance and metabolic diversity,microbes drive the cycling of essential elements at a planetary scale,sustain key symbiotic relationships with agricultural crops,and function as both sources and sinks of greenhouse gases.Thus,microorganisms must be recognized as indispensable agents within the Earth system,integral to understanding and addressing the dynamics of climate change.展开更多
Gastrointestinal malignancies,particularly pancreatobiliary and gastroesophageal cancers,are associated with poor prognosis due to their frequent late-stage diagnosis.Many of these tumors contribute to anorexia-cachex...Gastrointestinal malignancies,particularly pancreatobiliary and gastroesophageal cancers,are associated with poor prognosis due to their frequent late-stage diagnosis.Many of these tumors contribute to anorexia-cachexia syndrome and malnutrition,further exacerbating disease progression.Inflammation plays a crucial role in tumor proliferation,and growing evidence suggests that gut microbiome significantly influence inflammatory responses and clinical outcomes in these patients.Additionally,the gut microbiome contributes to carcinogenesis through multiple mechanisms,including DNA damage,activation of oncogenic pathways,and modulation of immune responses.The emerging field of nutritional interventions highlight the microbiome’s impact on anticancer drug responses,affecting both chemotherapy and molecular-targeted treatments.Given its pivotal role,microbiome modulation through probiotics,fecal microbiome transplantation,and antibiotics represents a promising approach for cancer pre-vention and treatment.In this review,we explore the intricate interplay between gut microbiome,inflammation,and nutritional status in gastrointestinal cancers,emphasizing potential therapeutic strategies to improve patient outcomes.展开更多
BACKGROUND Cognitive impairment is one of the common clinical manifestations of depression,causing negative distress to patients.Elevated homocysteine(Hcy)concentrations and gut microbiome dysfunction may be observed ...BACKGROUND Cognitive impairment is one of the common clinical manifestations of depression,causing negative distress to patients.Elevated homocysteine(Hcy)concentrations and gut microbiome dysfunction may be observed in patients with depression.AIM To investigate the relationship between Hcy,microbiome,and cognition in depressive patients.METHODS We recruited 67 patients with major depressive disorder(MDD)(MDD group)and 94 healthy controls(HCs)individuals(HCs group).Serum Hcy levels were determined using the enzyme circulation method.16s rRNA sequencing was used to classify and identify the fecal bacteria.17 Hamilton depression rating scale and MATRICS consensus cognitive battery were used to evaluate mood states and cognition in patients with MDD. Correlation analysis was performed to explore the correlation between fecal flora,Hcy, and depressive cognitive function.RESULTSElevated serum levels of Hcy were seen in patients with MDD compared to healthy individuals. Patients withMDD indicated significant decreases in cognitive scores (P < 0.001) in six modules: Speed of processing, workingmemory, visual learning, reasoning and problem-solving, social cognition, and total scores. Hcy levels showed anegative correlation with processing speed, social cognition, and total MDD scores (P < 0.05). Hcy was alsosignificantly negatively correlated with Alistipes, Ruminococcae, Tenericides, and Porphyromonas (P < 0.05).CONCLUSIONOur results highlight that Hcy was correlated with cognition and gut microbiome in MDD. This interaction may berelated to the physiological and pathological mechanisms underlying cognitive deficits in depression.展开更多
BACKGROUND The gut microbiome is associated with hepatic encephalopathy(HE),but research results on the gut microbiome characteristics of patients with liver cirrhosis with and without HE are inconsistent.AIM To study...BACKGROUND The gut microbiome is associated with hepatic encephalopathy(HE),but research results on the gut microbiome characteristics of patients with liver cirrhosis with and without HE are inconsistent.AIM To study the gut microbiota characteristics of patients with liver cirrhosis with and without HE.METHODS We searched the PubMed,Web of Science,EMBASE,and Cochrane databases using two keywords,HE,and gut microbiome.According to the inclusion and exclusion criteria,suitable literature was screened to extract data on the diversity and composition of the fecal microbiota in patients with liver cirrhosis with and without HE.The data were analyzed using RevMan and STATA.RESULTS Seventeen studies were included:(1)A meta-analysis of 7 studies revealed that the Shannon index in liver cirrhosis patients with HE was significantly lower than that in patients without HE[-0.20,95%confidence interval(CI):-0.28 to-0.13,I2=20%];(2)The relative abundances of Lachnospiraceae(-2.73,95%CI:-4.58 to-0.87,I2=38%)and Ruminococcaceae(-2.93,95%CI:-4.29 to-1.56,I2=0%)in liver cirrhosis patients with HE was significantly lower than those in patients without HE;(3)In patients with HE,Enterococcus,Proteobacteria,Enterococcaceae,and Enterobacteriaceae proportions increased,but Ruminococcaceae,Lachnospiraceae,Prevotellaceae,and Bacteroidetes proportions decreased;(4)Differences in the fecal metabolome between liver cirrhosis patients with and without HE were detected;and(5)Differential gut microbiomes may serve as diagnostic and prognostic tools.CONCLUSION The gut microbiomes of patients with liver cirrhosis with and without HE differ.Some gut microbiomes may distinguish liver cirrhosis patients with or without HE and determine patient prognosis.展开更多
The gut microbiome,a complex ecosystem of trillions of microorganisms,plays a crucial role in immune system regulation and overall health.This review explores the intricate cross-talk between the gut microbiota and th...The gut microbiome,a complex ecosystem of trillions of microorganisms,plays a crucial role in immune system regulation and overall health.This review explores the intricate cross-talk between the gut microbiota and the host immune system,emphasizing how microbial communities shape immune cell differentiation,modulate inflammatory responses,and contribute to immune homeostasis.Key interactions between innate and adaptive immune cells–including macrophages,dendritic cells,natural killer cells,innate Lymphoid cells,T cells,and B cells–and gut microbiota-derived metabolites such as short-chain fatty acids are discussed.The role of commensal bacteria in neonatal immune system development,mucosal barrier integrity,and systemic immunity is highlighted,along with implications for autoimmune diseases,inflammatory conditions,and cancer immunotherapy.Recent advances in metagenomics,metabolomics,and single-cell sequencing have provided deeper insights into the microbiota-immune axis,opening new avenues for microbiome-based therapeutic strategies.Understanding these interactions paves the way for novel interventions targeting immune-mediated diseases and optimizing health through microbiome modulation.展开更多
The oral microbiome is the second largest microbial community in the human body after the gut microbiome.It includes an array of fungi,bacteria,amoebae,flagellates,archaea,and viruses,all of which are potential pathog...The oral microbiome is the second largest microbial community in the human body after the gut microbiome.It includes an array of fungi,bacteria,amoebae,flagellates,archaea,and viruses,all of which are potential pathogens.This microbiome can act as a facilitator not only for protection but also for aggravation when dysbiosis occurs.Although conventional thought is this is primarily in terms of oral health issues,such as dental caries and gingival disease.The systemic effects of the oral microbiome however,are relevant to both gastrointestinal(GI)disease and non-GI disease.These systemic risks occur for several reasons,including upregulation of cytokines,adhesion cell-like processes,toll-like receptors,reactive oxidative species or generation of mutation inducing DNA changes.Additionally,there is translocation risk of potential active pathogens or their metabolic byproducts.There is a substantial and growing body of evidence that the oral microbiome influences diseases including Barrett’s esophagus,metabolicassociated steatosis liver disease,and GI cancers.Additionally,there is burgeoning evidence of a causal association with systemic inflammatory diseases,including inflammatory bowel disease.This report discusses the most recent evidence of this association and highlights new approaches to potentially enhance our“best practice”strategies for optimal care of patients with inflammatory bowel disease.展开更多
The gut microbiome plays a pivotal role in immune homeostasis and systemic inflammatory regulation,both of which are critically involved in the pathogenesis and progression of pediatric leukemias.Recent evidence revea...The gut microbiome plays a pivotal role in immune homeostasis and systemic inflammatory regulation,both of which are critically involved in the pathogenesis and progression of pediatric leukemias.Recent evidence reveals that children with leukemia often exhibit distinct gut microbiome profiles at diagnosis,marked by reduced microbial diversity and the enrichment of pro-inflammatory taxa such as Enterococcus and Streptococcus.This microbial dysbiosis may promote leukemogenesis by disrupting immune regulation and driving chronic inflammation.Chemotherapy significantly alters the gut microbiome,inducing dysbiosis characterized by a loss of beneficial commensals and the dominance of pathobionts.Specific microbial signatures,such as the enrichment of Bacteroides,correlate with reduced inflammation and improved prognosis,underscoring the gut microbiome's prognostic value.Emerging therapies,including dietary adjustments,probiotics,and fecal gut microbiome transplantation,aim to restore microbial balance and reduce treatment-related complications.Moreover,gut microbiome profiling shows potential for identifying biomarkers linked to leukemia predisposition,paving the way for early diagnosis and tailored preventive strategies.This mini-review explores recent advancements in understanding the influence of the gut microbiome on pediatric leukemias,emphasizing its role as both a therapeutic target and a prognostic biomarker.Integrating gut microbiome research into clinical practice may help optimize treatment outcomes and improve quality of life for children with leukemia.展开更多
基金supported by the National Key Research and Development Program of China(No.2022YFC3702704)the National Natural Science Foundation of China(Nos.22376005,22076006 and 82073506).
文摘Microorganisms constitute an essential component in the indoor environment,which is closely related to hu-man health.However,there is limited evidence regarding the associations between indoor airborne microbiome and systemic inflammation,as well as whether this association is modified by indoor particulate matter and the underlying mechanisms.In this prospective repeated-measure study among 66 participants,indoor airborne mi-crobiome was characterized using amplicon sequencing and qPCR.Indoor fine particulate matter(PM_(2.5))and inhalable particulate matter(PM10)were measured.Systemic inflammatory biomarkers were assessed,including white blood cell(WBC),neutrophil(NEUT),monocyte,eosinophil counts,and their proportions.Targeted serum amino acid metabolomics were conducted to explore the underlying mechanisms.Linear mixed-effect models re-vealed that bacterial and fungal Simpson diversity were significantly associated with decreased WBC and NEUT.For example,for each interquartile range increase in the bacterial Simpson diversity,WBC and NEUT changed by-4.53%(95%CI:-8.25%,-0.66%)and-5.95%(95%CI:-11.3%,-0.27%),respectively.Notably,increased inflammatory risks of airborne microbial exposure were observed when indoor PM_(2.5) and PM10 levels were below the WHO air quality guidelines.Mediation analyses indicated that dopamine metabolism partially mediated the anti-inflammatory effects of fungal diversity exposure.Overall,our study indicated protection from a diverse indoor microbial environment on cardiovascular health and proposed an underlying mechanism through amino acid metabolism.Additionally,health risks associated with microbial exposure deserve more attention in con-texts of low indoor particulate matter pollution.Further research is necessary to fully disentangle the complex relationships between indoor microbiome,air pollutants,and human health.
文摘The gut microbiome comprises a vast community of microbes inhabiting the human alimentary canal,playing a crucial role in various physiological functions.These microbes generally live in harmony with the host;however,when dysbiosis occurs,it can contribute to the pathogenesis of diseases,including osteoporosis.Osteoporosis,a systemic skeletal disease characterized by reduced bone mass and increased fracture risk,has attracted significant research attention concerning the role of gut microbes in its development.Advances in molecular biology have highlighted the influence of gut microbiota on osteoporosis through mechanisms involving immunoregulation,modulation of the gut-brain axis,and regulation of the intestinal barrier and nutrient absorption.These microbes can enhance bone mass by inhibiting osteoclast differentiation,inducing apoptosis,reducing bone resorption,and promoting osteoblast proliferation and maturation.Despite these promising findings,the therapeutic effectiveness of targeting gut microbes in osteoporosis requires further investigation.Notably,gut microbiota has been increasingly studied for their potential in early diagnosis,intervention,and as an adjunct therapy for osteoporosis,suggesting a growing utility in improving bone health.Further research is essential to fully elucidate the therapeutic potential and clinical application of gut microbiome modulation in the management of osteoporosis.
基金Supported by Liyang People's Hospital Technology Project,No.2021YJKT0013。
文摘BACKGROUND The gastric microbiome is closely associated with gastric cancer,and single-region 16S rRNA sequencing has limitations in analyzing its characteristics,necessitating the search for a better sequencing method.AIM To evaluate the effectiveness of multi-region 16S rRNA gene sequencing in studying the microbiome of gastric cancer tissues.METHODS Patients with gastric cancer(n=118)who underwent surgery at Liyang People's Hospital from January 2022 to December 2024 were enrolled.Fifty-nine paraffinembedded and 59 fresh tissue samples were obtained.The ZymoBIOMICSTM microbial community standard and Escherichia coli ATCC 25922 were used as positive controls.Multi-region and single-region 16S rRNA gene sequencing were performed.Species identification,detection rates at varying microbial abundances,operational taxonomic unit(OTU)counts,and alpha diversity indices in gastric cancer tissues were compared between the two methods.RESULTS Multi-region 16S rRNA sequencing identified more species(eight species and eight genera)in the positive controls compared with single-region sequencing(one species and six genera).Detection rates at concentrations of 103,102,and 10 CFU/mg were significantly higher using multi-region sequencing(P<0.05).Multi-region sequencing also revealed significantly higher OTU counts and alpha diversity indices(Shannon,Simpson,and Chao1)in gastric cancer tissues(P<0.05).CONCLUSION Compared with single-region sequencing,multi-region 16S rRNA gene sequencing demonstrates superior species resolution and detection sensitivity,providing a more comprehensive profile of microbial diversity in gastric cancer tissues.
基金jointly supported by the International Partnership Program of Chinese Academy of Sciences(161343KYSB20200015)Guangxi Provincial Natural Science Foundation of China(2023GXNSFAA026107)+1 种基金the Science and Technology Innovation Program of Hunan Province(2022RC1158)Youth Innovation Promotion Association CAS(2023382)。
文摘Background Dietary protein level and amino acid(AA)balance are crucial determinants of animal health and productivity.Supplementing rumen-protected AAs in low-protein diets was considered as an efficient strategy to improve the growth performance of ruminants.The colon serves as a crucial conduit for nutrient metabolism during rumen-protected methionine(RPMet)and rumen-protected lysine(RPLys)supplementation,however,it has been challenging to clarify which specific microbiota and their metabolites play a pivotal role in this process.Here,we applied metagenomic and metabolomic approaches to compare the characteristic microbiome and metabolic strategies in the colon of lambs fed a control diet(CON),a low-protein diet(LP)or a LP diet supplemented with RPMet and RPLys(LR).Results The LP treatment decreased the average daily weight gain(ADG)in lambs,while the LR treatment tended to elicit a remission in ADG.The butyrate molar concentration was greater(P<0.05),while acetate molar concentration(P<0.05)was lower for lambs fed the LP and LR diets compared to those fed the CON diet.Moreover,the LP treatment remarkably decreased total AA concentration(P<0.05),while LR treatment showed an improvement in the concentrations of methionine,lysine,leucine,glutamate,and tryptophan.Metagenomic insights proved that the microbial metabolic potentials referring to biosynthesis of volatile fatty acids(VFAs)and AAs in the colon were remarkably altered by three dietary treatments.Metagenomic binning identified distinct microbial markers for the CON group(Alistipes spp.,Phocaeicola spp.,and Ruminococcus spp.),LP group(Fibrobacter spp.,Prevotella spp.,Ruminococcus spp.,and Escherichia coli),and LR group(Akkermansia muciniphila and RUG099 spp.).Conclusions Our findings suggest that RPMet and RPLys supplementation to the low-protein diet could enhance the microbial biosynthesis of butyrate and amino acids,enriche the beneficial bacteria in the colon,and thereby improve the growth performance of lambs.
文摘The intricate interplay between natural compounds like curcumin and the gut microbiome has gained significant attention in recent years due to their potential therapeutic implications in various health conditions.Curcumin,a polyphenolic compound derived from turmeric,exhibits diverse pharmacological properties,including anti-inflammatory,antioxidant,and anticancer effects.Understanding how curcumin modulates gut microbiota composition and function is crucial for elucidating its therapeutic mechanisms.This review examines the current literature on the interactions between curcumin and the gut microbiome.A systematic search of relevant databases was conducted to identify studies investigating the effects of curcumin on gut microbial diversity and abundance.Key findings from studies exploring curcumin's efficacy in neurological disorders,gastrointestinal diseases,and metabolic dysfunction are synthesized and discussed.Studies have demonstrated that curcumin supplementation can modulate gut microbiota composition and function,leading to beneficial effects on gut health and homeostasis.Mechanisms underlying curcumin's therapeutic effects include immune modulation,neuroprotection,and inflammation regulation.However,challenges such as poor bioavailability and safety concerns remain significant hurdles to overcome.The interactions between curcumin and the gut microbiome hold promise for therapeutic interventions in a diverse range of health conditions.Further research is needed to optimize curcumin formulations,improve bioavailability,and address safety concerns.
文摘This study investigates the diversity of gut microbiota in Metaphire peguana,an earthworm species commonly found in agricultural areas of Thailand.Earthworms play a critical role in soil ecosystems by supporting nutrient cycling and breaking down organic matter.Understanding the microbial diversity in their gut is essential for exploring their ecological contributions.Using Next Generation Sequencing(NGS),we analyzed the mycobiome in the gut of M.peguana.Our findings revealed a high diversity of fungal species,primarily belonging to two major phyla:Ascomycota and Basidiomycota.Ascomycota was the most abundant phylum,comprising 40.1% of the total fungal species identified.A total of 33 distinct fungal species were identified,which underscores the richness of microbial life within the earthworm gut.This study successfully created the first genetic database of the microbial community in M.peguana,providing a foundation for future research in agricultural applications.The microbial species identified,particularly siderophoreproducing fungi,could have significant implications for improving soil fertility and promoting sustainable agricultural practices.The use of NGS technology has enabled comprehensive profiling of microbial communities,allowing for precise identification of fungi that may play essential roles in soil health.Furthermore,the study paves the way for future studies on the potential applications of earthworm gut microbiomes in biotechnology,especially in enhancing soil nutrient availability and plant growth.The findings of this research contribute to the broader understanding of the ecological roles of earthworms and their microbiomes in soil ecosystems.
基金The National Natural Science Foundation of China under contract Nos 42206157,42030502,and 42090041the Natural Science Foundation of Guangxi Province under contract No.2022GXNSFBA035449the Self-Topic Project of Guangxi Laboratory on the Study of Coral Reefs in the South China Sea under contract No.GXLSCRSCS2022103.
文摘The environments of tropical and subtropical coral reef regions(CRR)differ from each other;however,it is not known if these environmental differences influence coral polyp and skeleton microbiome composition.In this study,Coelastrea palauensis corals were collected from tropical and subtropical CRR in the South China Sea,and bacterial,archaeal,and fungal communities in polyps and skeletons were analyzed.Results showed that the microbial diversity and composition of C.palauensis significantly differed between the polyps and skeletons,and between the tropical and subtropical CRR.Regarding bacteria associated with corals,C.palauensis was mainly associated with bacteria closely related to the nitrogen cycle in the subtropical CRR.The relative abundances of Terasakiellaceae and Chlorobium in both coral polyps and skeletons in the subtropical CRR were higher than those in the tropical CRR.In the tropical CRR,C.palauensis was mainly associated with opportunistic pathogenic bacteria.The relative abundances of Tenacibaculum and Vibrio in coral polyps and skeletons in the tropical CRR were higher than those in the subtropical CRR.Regarding archaea associated with corals,polyps and skeletons of C.palauensis in both tropical and subtropical reef areas were dominated by n_Woesearchaeales,and the relative abundance of n_Woesearchaeales in skeletons is significantly higher than that in polyps.In addition,the relative abundances of n_Woesearchaeales in polyps and skeletons in the subtropical CRR were significantly higher than those in the tropical CRR.Regarding fungi associated with corals,Ascomycota was dominant in polyps and skeletons in the subtropical CRR,while Sordariomycetes,Periconia,Cladosporium,and Aspergillus were dominant in polyps and skeletons in the tropical CRR.Besides,the diversity differences of coral-associated microorganisms were related to environmental factors such as nutrients and temperature that may affect the survival of coral-associated microorganisms.These results implied that corals may adjust the composition of microorganisms,conducive the coral holobiont to better adapting the environment.Our research will be beneficial in understanding the differences and adaptations of coral polyp and skeletal microbiome.
文摘The community of microorganisms that colonize certain areas of the human body is called microbiota.Microorganisms such as bacteria,fungi and viruses make up the microbiota.The sum of the genomes of these microorganisms and microorganisms refers to the microbiome.It has been shown that microbiota has important effects such as protecting the organ from pathogens,contributing to metabolic functions(such as vitamin synthesis,carbohydrate digestion)and providing immunoregulation.Dysbiosis refers to compositional and functional changes in the microbiota.At the beginning of the 21st century,numerous studies have investigated the human microbiota and its imbalance in relation to various diseases and found that dysbiosis is associated with many diseases.The aim of this minireview article is to provide brief information about dysbiosis and its care and to raise awareness.
文摘BACKGROUND The gut microbiome is integral to human health,with emerging research underscoring its potential impact on ocular health through the gut-eye axis.Various ocular disorders,such as dry eye syndrome,retinal vascular diseases,macular degeneration,and glaucoma,may be influenced by gut dysbiosis,which could significantly contribute to their development and progression.AIM To evaluate the influence of the gut microbiome on the pathogenesis and progression of various ocular diseases.METHODS An extensive search of the scientific literature was undertaken by adhering to Preferred Reporting Items for Systematic Reviews&Meta-Analyses standards,using PubMed(MEDLINE),Scopus,EMBASE,and the Cochrane Library as sources to locate studies addressing the relationship between the gut microbiome and human health.To capture all relevant publications,search terms were systematically applied across these major databases,without limiting the search by language or publication date.Inclusion criteria covered randomized controlled trials,non-randomized controlled trial,prospective studies,cross-sectional studies,and case-control studies.Out of the 3077 articles,36 full texts were included in the review.RESULTS Ocular health appears to be shaped by the gut microbial community through mechanisms such as immune regulation,preservation of the blood–retinal barrier,and the generation of protective metabolites.Disturbances in this microbial balance can provoke measurable alterations in host immunity,providing a plausible immunopathogenic pathway that connects intestinal dysbiosis with eye disease.Both laboratory models and early human data suggest that targeted interventions,including prebiotics,probiotics,synbiotics,and faecal microbiota transfer,hold therapeutic potential.CONCLUSION The gut–eye relationship reflects a multifaceted interaction in which the intestinal microbiome contributes to ocular health through complex biological pathways.Integrating microbiome assessments into diagnostic methods can revolutionize disease management through early detection and targeted interventions.Further,randomised controlled clinical trials are necessary for ocular diseases to prove causal relationships.
基金supported by Science&Technology Fundamental Resources Investigation Program(2022FY100800)the CAMS Innovation Fund for Medical Sciences(CIFMS)(2021-12M-1-023/2023-12M-C&T-B-005)+1 种基金Funding for Reform and Development of Beijing Municipal Health Commissionthe National High Level Hospital Clinical Research Funding(2022-PUMCH-B-094).
文摘Menopause is characterized by the cessation of menstruation and a decline in reproductive function,which is an intrinsic component of the aging process.However,it has been a frequently overlooked field of women’s health.The oral and gut microbiota,constituting the largest ecosystem within the human body,are important for maintaining human health and notably contribute to the healthy aging of menopausal women.Therefore,a comprehensive review elucidating the impact of the gut and oral microbiota on menopause for healthy aging is of paramount importance.This paper presents the current understanding of the microbiome during menopause,with a particular focus on alterations in the oral and gut microbiota.Our study elucidates the complex interplay between the microbiome and sex hormone levels,explores microbial crosstalk dynamics,and investigates the associations between the microbiome and diseases linked to menopause.Additionally,this review explores the potential of microbiome-targeting therapies for managing menopause-related diseases.Given that menopause can last for approximately 30 years,gaining insights into how the microbiome and menopause interact could pave the way for innovative interventions,which may result in symptomatic relief from menopause and an increase in quality of life in women.
基金supported by the National Natural Science Foundation of China(Nos.42177008,and 42377005)the fellowship of China Postdoctoral Science Foundation(No.2022M712770)the Fundamental Research Funds for the Central Universities.
文摘Phyllosphere microbiome plays an irreplaceable role in maintaining plant health under stress,but its structure and functions in heavy metal-hyperaccumulating plants remain elusive.Here,the phyllosphere microbiome,inhabiting hyperaccumulating(HE)and non-hyperaccumulating ecotype(NHE)of Sedum alfredii grown in soils with varying heavy metal concentration,was characterized.Compared with NHE,the microbial communityα-diversity was greater in HE.Core phyllosphere taxa with high relative abundance(>10%),including Streptomyces and Nocardia(bacteria),Cladosporium and Acremonium(fungi),were significantly related to cadmium(Cd)and zinc(Zn)concentration and biomass of host plants.Moreover,microbial co-occurrence networks in HE exhibited greater complexity than those in NHE.Additionally,proportions of positive associations in HE bacterial networks increased with the rising heavy metal concentration,indicating a higher resistance of HE phyllosphere microbiome to heavy metal stress.Furthermore,in contrast to NHE,microbial community functions,primarily involved in heavy metal stress resistance,were more abundant in HE,in which microbiome assisted hosts to resist heavy metal stress better.Collectively,this study indicated that phyllosphere microbiome of the hyperaccumulator played an indispensable role in assisting hosts to resist heavy metal stress,and provided new insights into phyllosphere microbial application potential in phytoremediation.
文摘Atrial fibrillation(AF)is a growing global health burden,with a prevalence of over 52.55 million cases.Rising disability-adjusted life-years,increasing age,and disparities in care have contributed to the worsening severity and mortality of AF.Modifiable risk factors,such as hypertension,obesity,and diabetes mellitus,are associated with alterations in gut microbiota,making the gut-heart axis a potential therapeutic target.Gut dysbiosis influences AF pathogenesis through inflam-mation,metabolic disruption,and autonomic dysfunction.Key mechanisms include gut barrier dysfunction,short-chain fatty acid(SCFA)depletion,lipopoly-saccharides(LPS)-induced inflammation,and ferroptosis-mediated atrial remodeling.Trimethylamine N-oxide,bile acids,and tryptophan metabolites contribute to arrhythmogenic remodeling.Emerging evidence suggests that dietary interventions,including prebiotics and probiotics,as well as gut surveillance,may help mitigate AF progression.Clinical implications of gut modulation in AF include person-alized dietary strategies,microbiome assessment through metagenomic sequencing,and targeted interventions such as SCFA-based therapies and ferroptosis inhibition.Metabolite surveillance,including LPS and indoxyl sulfate monitoring,may influence the effectiveness of anticoagulant and antiarrhythmic therapy.Despite growing mechanistic evidence linking gut dysbiosis to AF,clinical applications remain unexplored.This review summarizes the current understanding of the gut microbiome's role in AF.
文摘The gut microbiome,a complex ecosystem of microorganisms,has a significant role in modulating pain,particularly within orthopaedic conditions.Its impact on immune and neurological functions is underscored by the gut-brain axis,which influences inflammation,pain perception,and systemic immune responses.This integrative review examines current research on how gut dysbiosis is associated with various pain pathways,notably nociceptive and neuroinflammatory mechanisms linked to central sensitization.We highlight advancements in meta-omics technologies,such as metagenomics and metaproteomics,which deepen our understanding of microbiome-host interactions and their implications in pain.Recent studies emphasize that gut-derived short-chain fatty acids and microbial metabolites play roles in modulating neuroinflammation and nociception,contributing to pain management.Probiotics,prebiotics,synbiotics,and faecal microbiome transplants are explored as potential therapeutic strategies to alleviate pain through gut microbiome modulation,offering an adjunct or alternative to opioids.However,variability in individual microbiomes poses challenges to standardizing these treatments,necessitating further rigorous clinical trials.A multidisciplinary approach combining microbiology,immunology,neurology,and orthopaedics is essential to develop innovative,personalized pain management strategies rooted in gut health,with potential to transform orthopaedic pain care.
基金supported by the China Social Science Foundation(24BZX097)and Noncommunicable Chronic Diseases-National Science and Technology Major Project(2023ZD0509602).
文摘Brief description Climate change represents an unparalleled existential threat to humanity in the twenty-first century,demanding urgent and sustained global attention.Among the vast array of actors shaping Earth’s climate system,microorganisms occupy a uniquely significant position.As the planet’s most abundant and diverse life forms,they not only respond sensitively to climatic change but also exert profound influence upon them.Microbes-comprising viruses,bacteria,archaea,fungi,algae,and protozoa-pervade from terrestrial soils and urban infrastructures to atmospheric layers,subterranean environments,and aquatic ecosystems.By virtue of their staggering abundance and metabolic diversity,microbes drive the cycling of essential elements at a planetary scale,sustain key symbiotic relationships with agricultural crops,and function as both sources and sinks of greenhouse gases.Thus,microorganisms must be recognized as indispensable agents within the Earth system,integral to understanding and addressing the dynamics of climate change.
文摘Gastrointestinal malignancies,particularly pancreatobiliary and gastroesophageal cancers,are associated with poor prognosis due to their frequent late-stage diagnosis.Many of these tumors contribute to anorexia-cachexia syndrome and malnutrition,further exacerbating disease progression.Inflammation plays a crucial role in tumor proliferation,and growing evidence suggests that gut microbiome significantly influence inflammatory responses and clinical outcomes in these patients.Additionally,the gut microbiome contributes to carcinogenesis through multiple mechanisms,including DNA damage,activation of oncogenic pathways,and modulation of immune responses.The emerging field of nutritional interventions highlight the microbiome’s impact on anticancer drug responses,affecting both chemotherapy and molecular-targeted treatments.Given its pivotal role,microbiome modulation through probiotics,fecal microbiome transplantation,and antibiotics represents a promising approach for cancer pre-vention and treatment.In this review,we explore the intricate interplay between gut microbiome,inflammation,and nutritional status in gastrointestinal cancers,emphasizing potential therapeutic strategies to improve patient outcomes.
基金Supported by the Wuxi Municipal Health Commission Youth Fund Project,No.Q202268Wuxi Scientific and technological breakthrough of“Light of the Taihu Lake”(Basic Research),No.K20221039+4 种基金Jiangsu Shuangchuang Doctoral Program,No.JSSCBS20221991Beijing Municipal Administration of Hospital Incubating Program,No.PX2023070 and No.PX2024072Capital’s Funds for Health Improvement and Research,No.SF2024-4-2134Beijing Hospitals Authority Youth Program,No.QML20232003the Top Talent Support Program for young and middle-aged people of Wuxi Health Committee,No.HB2023089.
文摘BACKGROUND Cognitive impairment is one of the common clinical manifestations of depression,causing negative distress to patients.Elevated homocysteine(Hcy)concentrations and gut microbiome dysfunction may be observed in patients with depression.AIM To investigate the relationship between Hcy,microbiome,and cognition in depressive patients.METHODS We recruited 67 patients with major depressive disorder(MDD)(MDD group)and 94 healthy controls(HCs)individuals(HCs group).Serum Hcy levels were determined using the enzyme circulation method.16s rRNA sequencing was used to classify and identify the fecal bacteria.17 Hamilton depression rating scale and MATRICS consensus cognitive battery were used to evaluate mood states and cognition in patients with MDD. Correlation analysis was performed to explore the correlation between fecal flora,Hcy, and depressive cognitive function.RESULTSElevated serum levels of Hcy were seen in patients with MDD compared to healthy individuals. Patients withMDD indicated significant decreases in cognitive scores (P < 0.001) in six modules: Speed of processing, workingmemory, visual learning, reasoning and problem-solving, social cognition, and total scores. Hcy levels showed anegative correlation with processing speed, social cognition, and total MDD scores (P < 0.05). Hcy was alsosignificantly negatively correlated with Alistipes, Ruminococcae, Tenericides, and Porphyromonas (P < 0.05).CONCLUSIONOur results highlight that Hcy was correlated with cognition and gut microbiome in MDD. This interaction may berelated to the physiological and pathological mechanisms underlying cognitive deficits in depression.
文摘BACKGROUND The gut microbiome is associated with hepatic encephalopathy(HE),but research results on the gut microbiome characteristics of patients with liver cirrhosis with and without HE are inconsistent.AIM To study the gut microbiota characteristics of patients with liver cirrhosis with and without HE.METHODS We searched the PubMed,Web of Science,EMBASE,and Cochrane databases using two keywords,HE,and gut microbiome.According to the inclusion and exclusion criteria,suitable literature was screened to extract data on the diversity and composition of the fecal microbiota in patients with liver cirrhosis with and without HE.The data were analyzed using RevMan and STATA.RESULTS Seventeen studies were included:(1)A meta-analysis of 7 studies revealed that the Shannon index in liver cirrhosis patients with HE was significantly lower than that in patients without HE[-0.20,95%confidence interval(CI):-0.28 to-0.13,I2=20%];(2)The relative abundances of Lachnospiraceae(-2.73,95%CI:-4.58 to-0.87,I2=38%)and Ruminococcaceae(-2.93,95%CI:-4.29 to-1.56,I2=0%)in liver cirrhosis patients with HE was significantly lower than those in patients without HE;(3)In patients with HE,Enterococcus,Proteobacteria,Enterococcaceae,and Enterobacteriaceae proportions increased,but Ruminococcaceae,Lachnospiraceae,Prevotellaceae,and Bacteroidetes proportions decreased;(4)Differences in the fecal metabolome between liver cirrhosis patients with and without HE were detected;and(5)Differential gut microbiomes may serve as diagnostic and prognostic tools.CONCLUSION The gut microbiomes of patients with liver cirrhosis with and without HE differ.Some gut microbiomes may distinguish liver cirrhosis patients with or without HE and determine patient prognosis.
文摘The gut microbiome,a complex ecosystem of trillions of microorganisms,plays a crucial role in immune system regulation and overall health.This review explores the intricate cross-talk between the gut microbiota and the host immune system,emphasizing how microbial communities shape immune cell differentiation,modulate inflammatory responses,and contribute to immune homeostasis.Key interactions between innate and adaptive immune cells–including macrophages,dendritic cells,natural killer cells,innate Lymphoid cells,T cells,and B cells–and gut microbiota-derived metabolites such as short-chain fatty acids are discussed.The role of commensal bacteria in neonatal immune system development,mucosal barrier integrity,and systemic immunity is highlighted,along with implications for autoimmune diseases,inflammatory conditions,and cancer immunotherapy.Recent advances in metagenomics,metabolomics,and single-cell sequencing have provided deeper insights into the microbiota-immune axis,opening new avenues for microbiome-based therapeutic strategies.Understanding these interactions paves the way for novel interventions targeting immune-mediated diseases and optimizing health through microbiome modulation.
文摘The oral microbiome is the second largest microbial community in the human body after the gut microbiome.It includes an array of fungi,bacteria,amoebae,flagellates,archaea,and viruses,all of which are potential pathogens.This microbiome can act as a facilitator not only for protection but also for aggravation when dysbiosis occurs.Although conventional thought is this is primarily in terms of oral health issues,such as dental caries and gingival disease.The systemic effects of the oral microbiome however,are relevant to both gastrointestinal(GI)disease and non-GI disease.These systemic risks occur for several reasons,including upregulation of cytokines,adhesion cell-like processes,toll-like receptors,reactive oxidative species or generation of mutation inducing DNA changes.Additionally,there is translocation risk of potential active pathogens or their metabolic byproducts.There is a substantial and growing body of evidence that the oral microbiome influences diseases including Barrett’s esophagus,metabolicassociated steatosis liver disease,and GI cancers.Additionally,there is burgeoning evidence of a causal association with systemic inflammatory diseases,including inflammatory bowel disease.This report discusses the most recent evidence of this association and highlights new approaches to potentially enhance our“best practice”strategies for optimal care of patients with inflammatory bowel disease.
文摘The gut microbiome plays a pivotal role in immune homeostasis and systemic inflammatory regulation,both of which are critically involved in the pathogenesis and progression of pediatric leukemias.Recent evidence reveals that children with leukemia often exhibit distinct gut microbiome profiles at diagnosis,marked by reduced microbial diversity and the enrichment of pro-inflammatory taxa such as Enterococcus and Streptococcus.This microbial dysbiosis may promote leukemogenesis by disrupting immune regulation and driving chronic inflammation.Chemotherapy significantly alters the gut microbiome,inducing dysbiosis characterized by a loss of beneficial commensals and the dominance of pathobionts.Specific microbial signatures,such as the enrichment of Bacteroides,correlate with reduced inflammation and improved prognosis,underscoring the gut microbiome's prognostic value.Emerging therapies,including dietary adjustments,probiotics,and fecal gut microbiome transplantation,aim to restore microbial balance and reduce treatment-related complications.Moreover,gut microbiome profiling shows potential for identifying biomarkers linked to leukemia predisposition,paving the way for early diagnosis and tailored preventive strategies.This mini-review explores recent advancements in understanding the influence of the gut microbiome on pediatric leukemias,emphasizing its role as both a therapeutic target and a prognostic biomarker.Integrating gut microbiome research into clinical practice may help optimize treatment outcomes and improve quality of life for children with leukemia.
