Objective:To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.Methods:Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was trans...Objective:To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.Methods:Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was transfected into 9S-C.The experiment group was divided into blank control group,empty vector transfected group and MicroRNA-lOb transfected group.Real time quantitative RT-PCR was used to detect the expression of MicroRNA-lOb and KLF4mRNA expression.Proliferations of cells were detected by cell proliferation assay,invasion of the delected the cell Transwell experiments,the expression of KLF4 protein was detected in Western blotting cells.Results:The proliferation rate of MicroRNA-lOb piasmid transfection group increased significantly after transfection,invasion and migration ability enhancement,by comparison,there are statistically significant differences in the blank control group and negative control group(P<0.05);the expression of MicroRNA-lOb piasmid transfection group KLF4protein decreased,the difference was statistically significant(P<0.05);reduce the expression of MicroRNA-l0b piasmid transfection group KLF4mRNA,but no significant differences(P>0.05).Conclusions:MicroRNA-l0b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.展开更多
基金supported by Sichuan Province Natural Science Foundation of China:(c271628)
文摘Objective:To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.Methods:Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was transfected into 9S-C.The experiment group was divided into blank control group,empty vector transfected group and MicroRNA-lOb transfected group.Real time quantitative RT-PCR was used to detect the expression of MicroRNA-lOb and KLF4mRNA expression.Proliferations of cells were detected by cell proliferation assay,invasion of the delected the cell Transwell experiments,the expression of KLF4 protein was detected in Western blotting cells.Results:The proliferation rate of MicroRNA-lOb piasmid transfection group increased significantly after transfection,invasion and migration ability enhancement,by comparison,there are statistically significant differences in the blank control group and negative control group(P<0.05);the expression of MicroRNA-lOb piasmid transfection group KLF4protein decreased,the difference was statistically significant(P<0.05);reduce the expression of MicroRNA-l0b piasmid transfection group KLF4mRNA,but no significant differences(P>0.05).Conclusions:MicroRNA-l0b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.
