摘要
目的探讨miRNA-10b对人肺腺癌细胞A549的增殖及侵袭能力的影响。方法用脂质体法将miRNA-10b真核表达质粒转染入A549,实验设置空白对照组、阴性对照组和miRNA-10b质粒转染组,转染后荧光显微镜下观察转染效率,实时定量PCR检测各组细胞miRNA-10b的表达,细胞增殖实验检测各组细胞的增殖情况,Transwell实验检测各组细胞侵袭能力。结果与空白对照组和阴性对照组相比,miRNA-10b质粒转染组细胞转染后增殖速度加快,细胞侵袭能力增强(P<0.05)。结论 miRNA-10b可以促进A549细胞的增殖及侵袭能力。
Objective To investigate the effects of miRNA -10b on proliferation and invasion of human lung adenocarci- noma cell line （A549）. Methods Under the induction of Lipofectamine TM 2000, the recombinant of miRNA-lOb was transfect- ed into A549. The experiment set up 3 groups：blank group, empty vector transfected group and miRNA-lOb expression plasmid transfected group. Transfection effciency was observed by fluorescence microscope, miRNA-t0b expression level was detected by real-time PCR after transfection, the cell proliferation was detected by cell proliferation assay ; the invasive ability of cells were de- tected by Transwell experiment. Results Compared with the blank control group and the empty vector transfection group, the cell proliferation rate increased obviously and the cell invasion enhanced in the microRNA-10b plasmid transfected group, the differ- ence was statistically significant （P 〈 0.05）. Conclusion miRNA-10b can promote the cell proliferation and increase the cell invasion ＇of A549.
出处
《实用癌症杂志》
2013年第5期469-471,共3页
The Practical Journal of Cancer
基金
新疆医科大学创新基金课题(XJC201156)
