Objective: Gut microbiome is an intricate micro-ecosystem mediating the human health and drug efficacy. Physalis alkekengi(PAL) is an edible and time-honored traditional Chinese medicine. Several pharmacological effec...Objective: Gut microbiome is an intricate micro-ecosystem mediating the human health and drug efficacy. Physalis alkekengi(PAL) is an edible and time-honored traditional Chinese medicine. Several pharmacological effects of PAL have been verified and gut bacteria are implied in its therapeutic actions.However, the detailed modulation of PAL on gut bacterial species and on gut fungi remains largely unknown. We, therefore, designed a preliminary experiment in normal mice to reveal the modulation effect of PAL on both gut bacteria and fungi, and explore the interaction between them.Methods: Herein, the aqueous extract of PAL was orally administrated to normal C57BL/6 mice for four weeks. The full-length 16S rRNA and ITS1/2 gene sequencing were explored to detect the taxa of gut bacteria and gut fungi after PAL treatment, respectively.Results: Oral administration of PAL notably enriched anti-infammatory bacterial species such as Duncaniella spp. and Kineothrix alysoides, whereas decreased pro-infammatory species such as Mucispirillum schaedleri. Simultaneously, PAL increased the abundance of gut fungi Aspergillus ochraceus,Cladosporium sp. and Alternaria sp., and decreased Penicillium janthinellum. Correlation network analysis identified two co-existing microbial groups(groups 1 and 2) that were negatively associated with each other. The group 1 comprised PAL-enriched bacteria and fungi, while group 2 was mainly normal chow-enriched bacteria and fungi. In group 1, Antrodia monomitica, Aspergillus clavatus, Mortierella kuhlmanii and Sarcinomyces sp. MA 4787 were positively correlated with Bifidobacterium globosum,Romboutsia ilealis and so on. In group 2, Chaetomium subspirilliferum, Septoria orchidearum and Cephaliophora tropica were positively related to Lactobacillus spp.Conclusion: Altogether, this preliminary study first demonstrated the modulation effect of PAL on both gut bacteria and gut fungi, which may shed light on the elucidation of PAL’s pharmacological mechanism.展开更多
氯仿(CHCl_(3))和溴仿(CHBr_(3))是两种重要的挥发性卤代烃(Volatile halohydrocarbon,VHCs),可通过光化学作用,引起臭氧层破坏和温室效应,影响全球气候变化。以黄河三角洲湿地的优势物种—盐地碱蓬(Suaeda glauca)为研究对象,采用室内...氯仿(CHCl_(3))和溴仿(CHBr_(3))是两种重要的挥发性卤代烃(Volatile halohydrocarbon,VHCs),可通过光化学作用,引起臭氧层破坏和温室效应,影响全球气候变化。以黄河三角洲湿地的优势物种—盐地碱蓬(Suaeda glauca)为研究对象,采用室内盆栽培养来探究不同浓度氮输入水平(CK,对照,6.0 g N m^(-2) a^(-1);1.5N0,低氮处理,9.0 g N m^(-2) a^(-1);3.0N0,高氮处理,18.0 g N m^(-2) a^(-1))对盐地碱蓬以及盆栽微生态系统CHCl_(3)、CHBr_(3)通量特征的影响。结果表明,不同氮梯度下盐地碱蓬CHCl_(3)和CHBr_(3)排放通量均呈现出先降后升的趋势,低浓度1.5N0下可以促进盐地碱蓬CHCl_(3)的释放,高浓度3.0N0则抑制;盐地碱蓬CHCl_(3)、CHBr_(3)气体通量的峰值分别出现在枯萎期与苗期,其主要原因是不同氮梯度刺激了气体消耗与产生之间的平衡以及植物各生长期生长因子的改变。由相关性分析可知,盐地碱蓬CHCl_(3)排放通量受多种因子相互作用,植物根长与CHBr_(3)排放之间存在显著相关性,根部越长,CHBr_(3)的排放通量越低。不同氮梯度下盆栽微生态系统内CHCl_(3)、CHBr_(3)排放通量的变化趋势不同,CHCl_(3)通量为先升后降,CHBr_(3)为先降后升,气体通量的最高值与最低值出现在植物不同生长时期,盆栽微生态系统气体排放通量主要受土壤NH^(+)_(4)-N、NO^(-)_(3)-N、以及植物凋落物等交互作用的影响。展开更多
基金supported financially by the National Natural Science Foundation of China (No. 81973217)。
文摘Objective: Gut microbiome is an intricate micro-ecosystem mediating the human health and drug efficacy. Physalis alkekengi(PAL) is an edible and time-honored traditional Chinese medicine. Several pharmacological effects of PAL have been verified and gut bacteria are implied in its therapeutic actions.However, the detailed modulation of PAL on gut bacterial species and on gut fungi remains largely unknown. We, therefore, designed a preliminary experiment in normal mice to reveal the modulation effect of PAL on both gut bacteria and fungi, and explore the interaction between them.Methods: Herein, the aqueous extract of PAL was orally administrated to normal C57BL/6 mice for four weeks. The full-length 16S rRNA and ITS1/2 gene sequencing were explored to detect the taxa of gut bacteria and gut fungi after PAL treatment, respectively.Results: Oral administration of PAL notably enriched anti-infammatory bacterial species such as Duncaniella spp. and Kineothrix alysoides, whereas decreased pro-infammatory species such as Mucispirillum schaedleri. Simultaneously, PAL increased the abundance of gut fungi Aspergillus ochraceus,Cladosporium sp. and Alternaria sp., and decreased Penicillium janthinellum. Correlation network analysis identified two co-existing microbial groups(groups 1 and 2) that were negatively associated with each other. The group 1 comprised PAL-enriched bacteria and fungi, while group 2 was mainly normal chow-enriched bacteria and fungi. In group 1, Antrodia monomitica, Aspergillus clavatus, Mortierella kuhlmanii and Sarcinomyces sp. MA 4787 were positively correlated with Bifidobacterium globosum,Romboutsia ilealis and so on. In group 2, Chaetomium subspirilliferum, Septoria orchidearum and Cephaliophora tropica were positively related to Lactobacillus spp.Conclusion: Altogether, this preliminary study first demonstrated the modulation effect of PAL on both gut bacteria and gut fungi, which may shed light on the elucidation of PAL’s pharmacological mechanism.
文摘氯仿(CHCl_(3))和溴仿(CHBr_(3))是两种重要的挥发性卤代烃(Volatile halohydrocarbon,VHCs),可通过光化学作用,引起臭氧层破坏和温室效应,影响全球气候变化。以黄河三角洲湿地的优势物种—盐地碱蓬(Suaeda glauca)为研究对象,采用室内盆栽培养来探究不同浓度氮输入水平(CK,对照,6.0 g N m^(-2) a^(-1);1.5N0,低氮处理,9.0 g N m^(-2) a^(-1);3.0N0,高氮处理,18.0 g N m^(-2) a^(-1))对盐地碱蓬以及盆栽微生态系统CHCl_(3)、CHBr_(3)通量特征的影响。结果表明,不同氮梯度下盐地碱蓬CHCl_(3)和CHBr_(3)排放通量均呈现出先降后升的趋势,低浓度1.5N0下可以促进盐地碱蓬CHCl_(3)的释放,高浓度3.0N0则抑制;盐地碱蓬CHCl_(3)、CHBr_(3)气体通量的峰值分别出现在枯萎期与苗期,其主要原因是不同氮梯度刺激了气体消耗与产生之间的平衡以及植物各生长期生长因子的改变。由相关性分析可知,盐地碱蓬CHCl_(3)排放通量受多种因子相互作用,植物根长与CHBr_(3)排放之间存在显著相关性,根部越长,CHBr_(3)的排放通量越低。不同氮梯度下盆栽微生态系统内CHCl_(3)、CHBr_(3)排放通量的变化趋势不同,CHCl_(3)通量为先升后降,CHBr_(3)为先降后升,气体通量的最高值与最低值出现在植物不同生长时期,盆栽微生态系统气体排放通量主要受土壤NH^(+)_(4)-N、NO^(-)_(3)-N、以及植物凋落物等交互作用的影响。
