目的 观察气管插管时不同气囊内压力下气管黏膜的病理改变.方法 选择气管内插管机械通气深昏迷患者60例,按随机原则分为囊内压25、30、35、40 mm Hg(1 mm Hg=0.133 kPa)组(分别记为A1、A2、A3、A4组),观察插管后1、24、48、72 h受压气...目的 观察气管插管时不同气囊内压力下气管黏膜的病理改变.方法 选择气管内插管机械通气深昏迷患者60例,按随机原则分为囊内压25、30、35、40 mm Hg(1 mm Hg=0.133 kPa)组(分别记为A1、A2、A3、A4组),观察插管后1、24、48、72 h受压气管黏膜病理改变.结果 4组患者气管黏膜均有不同程度病理改变,但组内比较差异均无统计学意义.随气囊内压增加,气管黏膜损伤加重;A1组气管黏膜病理改变轻微,均为Ⅰ度;A2组未发现Ⅲ度损伤.损伤1 h时,A3组Ⅱ度损伤例数较A2组明显增多(10例比6例,P【0.05);A4组Ⅱ度及Ⅲ度损伤例数与A3组比较差异有统计学意义(Ⅱ度:7例比10例,Ⅲ度:6例比2例,均P【0.05);而且损伤1 h后,损伤程度不随时间变化而加重.结论 气管插管机械通气气囊内压低于30 mm Hg时气管黏膜病理改变轻微,气管插管时气囊内压低于30 mm Hg较为妥当.展开更多
Raman spectroscopy offers non-destructive and highly sensitive molecular insights into bacterial species,making it a valuable tool for detection,identification,and antibiotic susceptibility testing.However,achieving c...Raman spectroscopy offers non-destructive and highly sensitive molecular insights into bacterial species,making it a valuable tool for detection,identification,and antibiotic susceptibility testing.However,achieving clinically relevant accuracy,quantitative data,and reproducibility remains challenging due to the dominance of bulk signals and the uncontrollable heterogeneity of analytes.In this study,we introduce an innovative diagnostic tool:a plasmonic fidget spinner(P-FS)incorporating a nitrocellulose membrane integrated with a metallic feature,referred to as a nanoplasmonic-enhanced matrix,designed for simultaneous bacterial filtration and detection.We developed a method to fabricate a plasmonic array patterned nitrocellulose membrane using photolithography,which is then integrated with a customized fidget spinner.Testing the P-FS device with various bacterial species(E.coli 25922,S.aureus 25923,E.coli MG1655,Lactobacillus brevis,and S.mutans 3065)demonstrated successful identification based on their unique Raman fingerprints.The bacterial interface with regions within the plasmonic array,where the electromagnetic field is most intensely concentrated—called nanoplasmonic hotspots—on the P-FS significantly enhances sensitivity,enabling more precise detection.SERS intensity mappings from the Raman spectrometer are transformed into digital signals using a threshold-based approach to identify and quantify bacterial distribution.Given the P-FS’s ability to enhance vibrational signatures and its scalable fabrication under routine conditions,we anticipate that nanoplasmonic-enhanced Raman spectroscopy—utilizing nanostructures made from metals(specifically gold and silver)deposited onto a nitrocellulose membrane to amplify Raman scattering signals—will become the preferred technology for reliable and ultrasensitive detection of various analytes,including those crucial to human health,with strong potential for transitioning from laboratory research to clinical applications.展开更多
文摘目的 观察气管插管时不同气囊内压力下气管黏膜的病理改变.方法 选择气管内插管机械通气深昏迷患者60例,按随机原则分为囊内压25、30、35、40 mm Hg(1 mm Hg=0.133 kPa)组(分别记为A1、A2、A3、A4组),观察插管后1、24、48、72 h受压气管黏膜病理改变.结果 4组患者气管黏膜均有不同程度病理改变,但组内比较差异均无统计学意义.随气囊内压增加,气管黏膜损伤加重;A1组气管黏膜病理改变轻微,均为Ⅰ度;A2组未发现Ⅲ度损伤.损伤1 h时,A3组Ⅱ度损伤例数较A2组明显增多(10例比6例,P【0.05);A4组Ⅱ度及Ⅲ度损伤例数与A3组比较差异有统计学意义(Ⅱ度:7例比10例,Ⅲ度:6例比2例,均P【0.05);而且损伤1 h后,损伤程度不随时间变化而加重.结论 气管插管机械通气气囊内压低于30 mm Hg时气管黏膜病理改变轻微,气管插管时气囊内压低于30 mm Hg较为妥当.
基金supported by the taxpayers of South Korea through the Institute for Basic Science(IBS-R020-D1)the Bio&Medical Technology Development Program of the National Research Foundation(NRF)funded by the Korean government(MSIT)(No.RS-2024-00508821).
文摘Raman spectroscopy offers non-destructive and highly sensitive molecular insights into bacterial species,making it a valuable tool for detection,identification,and antibiotic susceptibility testing.However,achieving clinically relevant accuracy,quantitative data,and reproducibility remains challenging due to the dominance of bulk signals and the uncontrollable heterogeneity of analytes.In this study,we introduce an innovative diagnostic tool:a plasmonic fidget spinner(P-FS)incorporating a nitrocellulose membrane integrated with a metallic feature,referred to as a nanoplasmonic-enhanced matrix,designed for simultaneous bacterial filtration and detection.We developed a method to fabricate a plasmonic array patterned nitrocellulose membrane using photolithography,which is then integrated with a customized fidget spinner.Testing the P-FS device with various bacterial species(E.coli 25922,S.aureus 25923,E.coli MG1655,Lactobacillus brevis,and S.mutans 3065)demonstrated successful identification based on their unique Raman fingerprints.The bacterial interface with regions within the plasmonic array,where the electromagnetic field is most intensely concentrated—called nanoplasmonic hotspots—on the P-FS significantly enhances sensitivity,enabling more precise detection.SERS intensity mappings from the Raman spectrometer are transformed into digital signals using a threshold-based approach to identify and quantify bacterial distribution.Given the P-FS’s ability to enhance vibrational signatures and its scalable fabrication under routine conditions,we anticipate that nanoplasmonic-enhanced Raman spectroscopy—utilizing nanostructures made from metals(specifically gold and silver)deposited onto a nitrocellulose membrane to amplify Raman scattering signals—will become the preferred technology for reliable and ultrasensitive detection of various analytes,including those crucial to human health,with strong potential for transitioning from laboratory research to clinical applications.
