Emerging evidence suggests that metabolic signals regulate mitochondrial homeostasis,with mitochondria-derived vesicles(MDVs)serving as a critical link between metabolites and mitochondrial quality control.In a recent...Emerging evidence suggests that metabolic signals regulate mitochondrial homeostasis,with mitochondria-derived vesicles(MDVs)serving as a critical link between metabolites and mitochondrial quality control.In a recent study,Tang et al.uncovered a novel mechanism in which metabolites modulate mitochondrial homeostasis throughβ-hydroxybutyrylation of sorting nexin 9(SNX9),thereby promoting MDV biogenesis[1].展开更多
Recombinant fowlpox viruses(rFPVs)coexpressing MDV gB and chicken type Ⅱ interferon (IFN-Ⅱ) gene were constructed by using different promoters of Ps and PE/L.rFPVs were selected and purified by blue plaque expressi...Recombinant fowlpox viruses(rFPVs)coexpressing MDV gB and chicken type Ⅱ interferon (IFN-Ⅱ) gene were constructed by using different promoters of Ps and PE/L.rFPVs were selected and purified by blue plaque expressing β-galactosidase.The expression of MDV gB gene was confirmed by indirect immunofluorescence assay.The levels of MDV gB expressed in rFPV-gB-IFN under the control of the synthetic promoter Ps was same as that in rFPV-gB.The expression of IFN-Ⅱ was examined in chicken embryo fibroblast(CEF) by protection against vesicular stomatitis virus-induced cytopathic effect.The levels of IFN expressed in CEF was 640～1280 units/mL.When rFPVs were propagated in CEF,the plaque sizes of rFPV-gB were larger than those of rFPV-gB-IFN,the plaque formation units of rFPV-gB were also more than those of rFPV-gB-IFN.展开更多
基金supported by research grants from the National Natural Science Foundation of China(32470795)the Guangdong Basic and Applied Basic Research Foundation(2025A1515010506 and 2022A1515111047)+4 种基金the Guangzhou Basic and Applied Basic Research Foundation(2023A04J1951)the Fundamental Research Funds for the Central UniversitiesSun Yat-sen University(24pnpy249)supported by the following research grants from University of Macao(CPG2025-0031-FHS and UM-MYRG2020-00022-FHS)the Macao Science and Technology Development Fund(FDCT0078/2020/A2,FDCT0031/2021/A1,FDCT0081/2022/AMJ,and FDCT 0004/2021/AKP)。
文摘Emerging evidence suggests that metabolic signals regulate mitochondrial homeostasis,with mitochondria-derived vesicles(MDVs)serving as a critical link between metabolites and mitochondrial quality control.In a recent study,Tang et al.uncovered a novel mechanism in which metabolites modulate mitochondrial homeostasis throughβ-hydroxybutyrylation of sorting nexin 9(SNX9),thereby promoting MDV biogenesis[1].
文摘Recombinant fowlpox viruses(rFPVs)coexpressing MDV gB and chicken type Ⅱ interferon (IFN-Ⅱ) gene were constructed by using different promoters of Ps and PE/L.rFPVs were selected and purified by blue plaque expressing β-galactosidase.The expression of MDV gB gene was confirmed by indirect immunofluorescence assay.The levels of MDV gB expressed in rFPV-gB-IFN under the control of the synthetic promoter Ps was same as that in rFPV-gB.The expression of IFN-Ⅱ was examined in chicken embryo fibroblast(CEF) by protection against vesicular stomatitis virus-induced cytopathic effect.The levels of IFN expressed in CEF was 640～1280 units/mL.When rFPVs were propagated in CEF,the plaque sizes of rFPV-gB were larger than those of rFPV-gB-IFN,the plaque formation units of rFPV-gB were also more than those of rFPV-gB-IFN.
