Bacteriostasic activity experiments on anthocyanin extracted from Malva sylvestris inhibiting Staphylococcus aureus, Escherichia coli, Aspergillus niger, were conducted by using solid and liquid culture methods. The r...Bacteriostasic activity experiments on anthocyanin extracted from Malva sylvestris inhibiting Staphylococcus aureus, Escherichia coli, Aspergillus niger, were conducted by using solid and liquid culture methods. The results showed that the anthocyanin of M. sylvestris had a great bacteriostasic activity to Staphylococcus aureus but had no bacteriostasic activity to both Escherichia coli and Aspergillus niger. The bacteriostasic activity to Staphylococcus aureus increased with increasing content of anthocyanin of Malva Sylvestris in the solid-culture experiment. The average diameters of bacteriostasic circle for Staphylococcus aureus was 6, 13.5, and 16.0 mm at 10g·L^-1 , 20g· L^-1, and 30g· L^-1 contents of anthocyanin of M. sylvestris, respectively, Moreover, this bacteriostasic activity kept long time as anthocyanin was of the high concentration.展开更多
Objective:To guide for selection of plants with antibacterial activity for further phytochemical works on the isolation and identification of the active compounds.Methods:Ethanolic extracts of 3 species from Malvaceae...Objective:To guide for selection of plants with antibacterial activity for further phytochemical works on the isolation and identification of the active compounds.Methods:Ethanolic extracts of 3 species from Malvaceae family were evaluated by agar disc diffusion method for antibacterial activity against some gram-positive and gram-negative bacteria(Pseudomonas aeruginosa,Listeria monocytogenes,Staphylococcus epidermidis.Staphylococcus aureus, Klebsiella pneumoniae.Salmonella typhi,Bacillus cereus.Bacillus anthracis,Escherichia coli and Streptococcus pyogenes).The extracts were obtained from aerial parts of Hibiscus rosa(H. rosa)-sinensis(leaf and flower),Alcea rosea(A.rosea) L.(leaf and flower) and Malva neglecta(M. neglecta) Wallr(flower).Results:These extracts had inhibitory effects at different concentrations (0.05,0.10,0.20 and 0.40 g/mL) against above mentioned bacteria.Escherichia coli was the most resistant strain.The highest inhibitory zone was showed by ethanolic extract of M.neglecta against Staphylococcus epidermidis(22 mm) and followed by ethanolic extract from flower of H.rosa against Staphylococcus epidermidis and Staphylococcus aureus(20 mm).The Minimum Inhibitory Concentration(MIC) and Minimum Bactericidal Concentration(MBC) values against Staphylococcus epidermidis were equal(M1C=MBC=5 mg/mL for M.neglecta extract and for H. rosa extract MIC=MBC=20 mg/mL).Conclusions:These findings suggest that these native plants have good antibacterial properties that can be used for infection control and treatment and could also be as new source for antibiotics discovery and infection treatment.展开更多
Objective: The aim of this study was to evaluate the analgesic and anti-inflammatory effects of the hydroalcoholic extracts of Malva sylvestris flowers or Carum carvi and Medicago sativa seeds, alone and in combinatio...Objective: The aim of this study was to evaluate the analgesic and anti-inflammatory effects of the hydroalcoholic extracts of Malva sylvestris flowers or Carum carvi and Medicago sativa seeds, alone and in combination, which have been used in traditional Iranian medicine.Methods: Male Wistar rats were divided into 6 treatment groups: distilled water, sodium salicylate(SS),M. sylvestris extract(600 mg/kg), C. carvi extract(600 mg/kg), M. sativa extract(300 mg/kg) and combined extract(including 300 mg/kg M. sylvestris and C. carvi extracts, and 150 mg/kg M. sativa extract). The formalin pain model was used to evaluate the antinociceptive effects of the treatments. For anti-inflammatory effect, acute(one hour after injection) and chronic(during a week after injection)paw inflammation was measured after subcutaneous injection of 2.5% formalin in the hindpaw.Finally, tissue samples from all groups were prepared for histopathological studies.Results: The combined extract significantly inhibited the nociception in the acute phase of the formalin test(P < 0.001). In the chronic phase, all the extracts and SS had significant analgesic effect(P < 0.001).Analgesic activity of the combined extract was significantly stronger than SS(P < 0.01). In the acute inflammation model, M. sylvestris, C. carvi and the combined drug had significant inhibitory effects against paw edema(P < 0.05). All extracts, individually and in combination, significantly alleviated chronic paw inflammation(P < 0.01). The combined extract had much more anti-inflammatory activity than SS(P < 0.05). Histopathological results indicated improvement and reduction of inflammatory factors in the treatment groups.Conclusion: M. sylvestris, C. carvi and M. sativa have analgesic and anti-inflammatory properties.Potentially, each of these extracts or a mixture of them might be a valuable alternative drug to control pain and inflammation.展开更多
Four short-term in vivo and in vitro tests were used to further confirm the antitumor activities of MCP, a vegetable powder, prepared from Malru crispo L. (i) In the H22 hepatoma-transplanting test, MCP had antitumor ...Four short-term in vivo and in vitro tests were used to further confirm the antitumor activities of MCP, a vegetable powder, prepared from Malru crispo L. (i) In the H22 hepatoma-transplanting test, MCP had antitumor action, but MCP residue did not show such action; 5-FU appeared to have more potent antitumor activities and more harmful effects than MCP. (ii) In the micronucleus (MN) test, MCP significantly decreased MN frequency. (iii) In the cancer cell culture systems, the MCP fat-soluble extract revealed inhibitory effects on the growth and proliferation of the human hepatoma and the gastric cancer cells in a dose-response manner. (iv) In the colony formation test, MCP also altered the morphology of human gastric cancer cells. It was suggested that MCP could be consumed not only by healthy subJects for cancer prevention but also by patients with cancer as supplernentary treatrnent in combination with anticarcinogenic drug such as 5-FU, cyclophosphamide (CP)展开更多
Objective:To establish quality control parameters of a locally occurring medicinal plant,Malva parviflora which is utilized as folk medicine in Sialkot area in Pakistan.Methods:In pharmacognostic studies different typ...Objective:To establish quality control parameters of a locally occurring medicinal plant,Malva parviflora which is utilized as folk medicine in Sialkot area in Pakistan.Methods:In pharmacognostic studies different types of evaluations were carried out that focus on microscopic,macroscopic,fluorescence analysis and organoleptic evaluations.Results:The distinguishing characters of stem were the presence of parenchyma,cork cells,irregular shape calcium oxalate crystals,simple and compound starch granules and fusiform fibers with pits.Root microscopic characters were presence of simple and spherical starch granules with rounded or slit hilum,groups of lignified xylem fibers,reticulate vessek,and sieve tissues.Leaves microscopy indicated the presence of paracytic stomata,lignified fibers having pits,spiral and annular vessels,numerous sclereids while in fruit microscopy epicarp,thin walled cells endocarp,thin walled parenchyma and collenchyma of mesocarp and abundant thick walled endospermic cells containing aleurone grains and micro rosette crystals.Macroscopic study of leaves showed,5-7 lobed reniform-shape,glabrous-surface,reticulate-venation in the leaves.Macroscopic features of roots showed type of root-taproot,surface-glabrous and stem was 1-10dm tall simple to branched and may be prostrate or ascending.Similarly fruit was of schizocarp type.Conclusions:This study provides the scientific data for the proper identification and establishment of standards for the use of Malva parviflora.展开更多
The objective of this research was to investigate the effect of Malva nut gum （MG） replacement on the pasting characteristics and freeze-thaw stability of wheat, rice or waxy rice flours. Pasting properties and free...The objective of this research was to investigate the effect of Malva nut gum （MG） replacement on the pasting characteristics and freeze-thaw stability of wheat, rice or waxy rice flours. Pasting properties and freeze-thaw stability of different flours incorporated with 0, 0.5%, 1%, 2%, 3% and 5% of MG were investigated. Pasting temperature （60 ℃-87 ℃） of the pastes significantly decreased with increasing of MG content for wheat and rice flours, but had no significant effect for waxy rice flour. Incorporation of MG into all flours significantly elevated the peak viscosity by about 0.9-2.6 folds when compared to non-MG samples. Hot paste viscosity, breakdown and final viscosity for all flour mixtures significantly increased with increasing of MG which ranged from 81-427, 37-559 and 152-463 RVU, respectively. Freeze-thaw stability measurement demonstrated that higher level of MG in wheat and rice gel mixtures could decrease syneresis. However, MG had no effect on syneresis of waxy rice gel. Presence of MG in flours alters the pasting properties and syneresis effect. It is suggested that higher viscosity and lower syneresis of gels could be modified by MG.展开更多
To the Editor: Dietary supplements and herbal products are an increasingly common source of drug-induced liver injury. An increasing number of case reports have suggested causative supplement-associated liver toxicity...To the Editor: Dietary supplements and herbal products are an increasingly common source of drug-induced liver injury. An increasing number of case reports have suggested causative supplement-associated liver toxicity. [1] Malva grandifolia is a plant which is frequently found in flower and vegetable gardens (Fig.). Mallow products are used展开更多
This study investigates the fermentation of Malva sylvestris aerial parts using three lactic acid bacteria:Lactiplantibacillus plantarum 299V,Pediococcus acidilactici IRZ12B,and Lacticaseibacillus rhamnosus GG.The fer...This study investigates the fermentation of Malva sylvestris aerial parts using three lactic acid bacteria:Lactiplantibacillus plantarum 299V,Pediococcus acidilactici IRZ12B,and Lacticaseibacillus rhamnosus GG.The fermentation process resulted in a notable increase in microbial counts of LAB populations,production of organic acids,reduction of antinutritional factors such as tannins,and enhancement the bioavailability of some essential minerals.Post-fermentation analyses revealed a threefold increase in total phenolic content compared to the non-fermented extract.Antioxidant activity also showed a substantial enhancement,with L.plantarum 299V and P.acidilactici IRZ12B nearly doubling the DPPH inhibition percentage,while L.rhamnosus GG exhibited no improvement.Furthermore,antimicrobial activity varied among strains,with P.acidilactici IRZ12B and L.rhamnosus GG effectively inhibiting Y.enterocolitica growth,while all fermented samples significantly reduced S.enterica proliferation.These findings support the use of lactic acid bacteria fermentation as a bioprocess to improve the phytochemical profile of malva,with potential therapeutic applications.Furthermore,the optimized fermentation conditions could facilitate the incorporation of fermented malva as a functional ingredient in plant-based foods,aligning with the growing consumer demand for health-oriented products.展开更多
文摘Bacteriostasic activity experiments on anthocyanin extracted from Malva sylvestris inhibiting Staphylococcus aureus, Escherichia coli, Aspergillus niger, were conducted by using solid and liquid culture methods. The results showed that the anthocyanin of M. sylvestris had a great bacteriostasic activity to Staphylococcus aureus but had no bacteriostasic activity to both Escherichia coli and Aspergillus niger. The bacteriostasic activity to Staphylococcus aureus increased with increasing content of anthocyanin of Malva Sylvestris in the solid-culture experiment. The average diameters of bacteriostasic circle for Staphylococcus aureus was 6, 13.5, and 16.0 mm at 10g·L^-1 , 20g· L^-1, and 30g· L^-1 contents of anthocyanin of M. sylvestris, respectively, Moreover, this bacteriostasic activity kept long time as anthocyanin was of the high concentration.
基金Shahid Chamran University for preparing research grant
文摘Objective:To guide for selection of plants with antibacterial activity for further phytochemical works on the isolation and identification of the active compounds.Methods:Ethanolic extracts of 3 species from Malvaceae family were evaluated by agar disc diffusion method for antibacterial activity against some gram-positive and gram-negative bacteria(Pseudomonas aeruginosa,Listeria monocytogenes,Staphylococcus epidermidis.Staphylococcus aureus, Klebsiella pneumoniae.Salmonella typhi,Bacillus cereus.Bacillus anthracis,Escherichia coli and Streptococcus pyogenes).The extracts were obtained from aerial parts of Hibiscus rosa(H. rosa)-sinensis(leaf and flower),Alcea rosea(A.rosea) L.(leaf and flower) and Malva neglecta(M. neglecta) Wallr(flower).Results:These extracts had inhibitory effects at different concentrations (0.05,0.10,0.20 and 0.40 g/mL) against above mentioned bacteria.Escherichia coli was the most resistant strain.The highest inhibitory zone was showed by ethanolic extract of M.neglecta against Staphylococcus epidermidis(22 mm) and followed by ethanolic extract from flower of H.rosa against Staphylococcus epidermidis and Staphylococcus aureus(20 mm).The Minimum Inhibitory Concentration(MIC) and Minimum Bactericidal Concentration(MBC) values against Staphylococcus epidermidis were equal(M1C=MBC=5 mg/mL for M.neglecta extract and for H. rosa extract MIC=MBC=20 mg/mL).Conclusions:These findings suggest that these native plants have good antibacterial properties that can be used for infection control and treatment and could also be as new source for antibiotics discovery and infection treatment.
文摘Objective: The aim of this study was to evaluate the analgesic and anti-inflammatory effects of the hydroalcoholic extracts of Malva sylvestris flowers or Carum carvi and Medicago sativa seeds, alone and in combination, which have been used in traditional Iranian medicine.Methods: Male Wistar rats were divided into 6 treatment groups: distilled water, sodium salicylate(SS),M. sylvestris extract(600 mg/kg), C. carvi extract(600 mg/kg), M. sativa extract(300 mg/kg) and combined extract(including 300 mg/kg M. sylvestris and C. carvi extracts, and 150 mg/kg M. sativa extract). The formalin pain model was used to evaluate the antinociceptive effects of the treatments. For anti-inflammatory effect, acute(one hour after injection) and chronic(during a week after injection)paw inflammation was measured after subcutaneous injection of 2.5% formalin in the hindpaw.Finally, tissue samples from all groups were prepared for histopathological studies.Results: The combined extract significantly inhibited the nociception in the acute phase of the formalin test(P < 0.001). In the chronic phase, all the extracts and SS had significant analgesic effect(P < 0.001).Analgesic activity of the combined extract was significantly stronger than SS(P < 0.01). In the acute inflammation model, M. sylvestris, C. carvi and the combined drug had significant inhibitory effects against paw edema(P < 0.05). All extracts, individually and in combination, significantly alleviated chronic paw inflammation(P < 0.01). The combined extract had much more anti-inflammatory activity than SS(P < 0.05). Histopathological results indicated improvement and reduction of inflammatory factors in the treatment groups.Conclusion: M. sylvestris, C. carvi and M. sativa have analgesic and anti-inflammatory properties.Potentially, each of these extracts or a mixture of them might be a valuable alternative drug to control pain and inflammation.
文摘Four short-term in vivo and in vitro tests were used to further confirm the antitumor activities of MCP, a vegetable powder, prepared from Malru crispo L. (i) In the H22 hepatoma-transplanting test, MCP had antitumor action, but MCP residue did not show such action; 5-FU appeared to have more potent antitumor activities and more harmful effects than MCP. (ii) In the micronucleus (MN) test, MCP significantly decreased MN frequency. (iii) In the cancer cell culture systems, the MCP fat-soluble extract revealed inhibitory effects on the growth and proliferation of the human hepatoma and the gastric cancer cells in a dose-response manner. (iv) In the colony formation test, MCP also altered the morphology of human gastric cancer cells. It was suggested that MCP could be consumed not only by healthy subJects for cancer prevention but also by patients with cancer as supplernentary treatrnent in combination with anticarcinogenic drug such as 5-FU, cyclophosphamide (CP)
基金M.Phil degree project and was fully funded under the grant number 36-79/155 by University College of Pharmacy,University of the Punjab,Lahore,PakistanTechnical support is also provided by GCU,Lahore,Pakistan
文摘Objective:To establish quality control parameters of a locally occurring medicinal plant,Malva parviflora which is utilized as folk medicine in Sialkot area in Pakistan.Methods:In pharmacognostic studies different types of evaluations were carried out that focus on microscopic,macroscopic,fluorescence analysis and organoleptic evaluations.Results:The distinguishing characters of stem were the presence of parenchyma,cork cells,irregular shape calcium oxalate crystals,simple and compound starch granules and fusiform fibers with pits.Root microscopic characters were presence of simple and spherical starch granules with rounded or slit hilum,groups of lignified xylem fibers,reticulate vessek,and sieve tissues.Leaves microscopy indicated the presence of paracytic stomata,lignified fibers having pits,spiral and annular vessels,numerous sclereids while in fruit microscopy epicarp,thin walled cells endocarp,thin walled parenchyma and collenchyma of mesocarp and abundant thick walled endospermic cells containing aleurone grains and micro rosette crystals.Macroscopic study of leaves showed,5-7 lobed reniform-shape,glabrous-surface,reticulate-venation in the leaves.Macroscopic features of roots showed type of root-taproot,surface-glabrous and stem was 1-10dm tall simple to branched and may be prostrate or ascending.Similarly fruit was of schizocarp type.Conclusions:This study provides the scientific data for the proper identification and establishment of standards for the use of Malva parviflora.
文摘The objective of this research was to investigate the effect of Malva nut gum （MG） replacement on the pasting characteristics and freeze-thaw stability of wheat, rice or waxy rice flours. Pasting properties and freeze-thaw stability of different flours incorporated with 0, 0.5%, 1%, 2%, 3% and 5% of MG were investigated. Pasting temperature （60 ℃-87 ℃） of the pastes significantly decreased with increasing of MG content for wheat and rice flours, but had no significant effect for waxy rice flour. Incorporation of MG into all flours significantly elevated the peak viscosity by about 0.9-2.6 folds when compared to non-MG samples. Hot paste viscosity, breakdown and final viscosity for all flour mixtures significantly increased with increasing of MG which ranged from 81-427, 37-559 and 152-463 RVU, respectively. Freeze-thaw stability measurement demonstrated that higher level of MG in wheat and rice gel mixtures could decrease syneresis. However, MG had no effect on syneresis of waxy rice gel. Presence of MG in flours alters the pasting properties and syneresis effect. It is suggested that higher viscosity and lower syneresis of gels could be modified by MG.
文摘To the Editor: Dietary supplements and herbal products are an increasingly common source of drug-induced liver injury. An increasing number of case reports have suggested causative supplement-associated liver toxicity. [1] Malva grandifolia is a plant which is frequently found in flower and vegetable gardens (Fig.). Mallow products are used
文摘This study investigates the fermentation of Malva sylvestris aerial parts using three lactic acid bacteria:Lactiplantibacillus plantarum 299V,Pediococcus acidilactici IRZ12B,and Lacticaseibacillus rhamnosus GG.The fermentation process resulted in a notable increase in microbial counts of LAB populations,production of organic acids,reduction of antinutritional factors such as tannins,and enhancement the bioavailability of some essential minerals.Post-fermentation analyses revealed a threefold increase in total phenolic content compared to the non-fermented extract.Antioxidant activity also showed a substantial enhancement,with L.plantarum 299V and P.acidilactici IRZ12B nearly doubling the DPPH inhibition percentage,while L.rhamnosus GG exhibited no improvement.Furthermore,antimicrobial activity varied among strains,with P.acidilactici IRZ12B and L.rhamnosus GG effectively inhibiting Y.enterocolitica growth,while all fermented samples significantly reduced S.enterica proliferation.These findings support the use of lactic acid bacteria fermentation as a bioprocess to improve the phytochemical profile of malva,with potential therapeutic applications.Furthermore,the optimized fermentation conditions could facilitate the incorporation of fermented malva as a functional ingredient in plant-based foods,aligning with the growing consumer demand for health-oriented products.
