In this research, Lysolecithin-a substance made with 100% natural ingredients - was given to ICR mice as medication to measure its periodic effect on the noradrenalin (NA), dopamine (DA), and serotonin (5-HT) levels o...In this research, Lysolecithin-a substance made with 100% natural ingredients - was given to ICR mice as medication to measure its periodic effect on the noradrenalin (NA), dopamine (DA), and serotonin (5-HT) levels of the brain. Both ICR and SAM mice were separated into two groups - control group and Lysolecithin (K. Lysolecithin: hydrolytic lysolecithin) medicated group, and given 1-week preparation period. The K. Lysolecithin group was given 500mg/kg of K. Lysolecithin at 0.2mL per dosage for 4 weeks, and the control group was given the same amount of dosage of water during the same period. NA, DA and 5-HT concentrations were measured from the blood before medication and 8 weeks / 12 weeks / 16 weeks after the first medication. For the SAM mice, 8 weeks after they were medicated with K .Lysolecithin, Morris Water Maze Test was conducted for 7 consecutive days and then the concentrations were measured by drawing blood from the heart. The K. Lysolecithin medicated group showed a tendency to have a statistically significant higher concentrations of 5-HT and NA in the blood. Also, periodic examination showed that the monoamine levels were highest in the 12th week and declined thereafter.展开更多
An 8-week trial was conducted to investigate graded levels of dietary LPC on growth,apparent digestibility(ADs)of nutrients and lipid metabolism in juvenile turbot Scophthalmus maximus L.Five experimental diets were f...An 8-week trial was conducted to investigate graded levels of dietary LPC on growth,apparent digestibility(ADs)of nutrients and lipid metabolism in juvenile turbot Scophthalmus maximus L.Five experimental diets were formulated which contain 11%crude fat with graded amounts of LPC(0,1000,2500,4000,5500 mg/kg diet).The fish fed diet with 11%crude lipid and 0 LPC was used as the negative control group(C-),meanwhile the fish fed diet with 12%crude fat and 0 LPC was used as the positive control group(C+).Each diet was fed to triplicate groups of turbot(initial body weight of 41 g)for 56d.Weight gain rate and specific growth rate of the fish fed LPC supplemented diets were significantly higher than the control groups,and the growth performance of the C-group was significantly lower than the others.The viscerosomatic index(VSI)was significantly decreased and the gall bladder somatic index(GSI)was significantly increased by dietary LPC.Crude lipid contents of muscle were elevated by dietary LPC.Apparent digestibility of energy was decreased by dietary LPC,but protein and lipid were not affected.Both total cholesterol(T-CHO)and high density lipoprotein cholesterol(HDL-C)were up-regulated,meanwhile neither alanine aminotransferase(ALT)nor total protein(TP)were down-regulated by dietary LPC.Enzymes involved in lipid metabolism(total lipase,hepatic lipase,lipoprotein lipase,fatty acids synthase,and lipase)were all elevated by dietary LPC.In conclusion,the lipid requirements of turbot were decreased by dietary LPC and the lipid utilization coefficient was enhanced.Based on SAS NLIN regression,dietary 870.37 mg/kg LPC was appropriate for turbot juvenile.展开更多
Porphyromonas gingivalis is a notorious keystone periodontal pathogen.However,it is difficult to selectively inhibit its growth in the oral cavity.Thereby,liposome-based antibacterial agents were investigated to suppr...Porphyromonas gingivalis is a notorious keystone periodontal pathogen.However,it is difficult to selectively inhibit its growth in the oral cavity.Thereby,liposome-based antibacterial agents were investigated to suppress the growth of P.gingivalis.The antibacterial activities of enzymatically prepared lysolecithin-fatty acid liposomes(Lyso-FAL)were comparable to those of free-form linoleic acid in a simple aqueous system with a minimum inhibitory concentration(MIC)of 4.8μg/mL.However,this value was 2.8-fold lower than those of the lecithin liposomes including the same amount of free fatty acids(e.g.,linoleic acid).This indicated that the lysolecithin liposomes are very efficient in the delivery of free fatty acids from the aqueous medium to P.gingivalis.Remarkably,the antibacterial activities in the presence of milk and milk alternatives were significantly stronger(MIC,35μg/mL)than free-form linoleic acid(MIC,≥100μg/mL).The higher antibacterial activities of the liposomal fatty acids might be due to the efficient delivery of the antibacterial fatty acids from the liposomes to P.gingivalis even in the presence of emulsified fat globules and protein micelles in milk and milk substitutes.Besides,the lysolecithin-free fatty acid liposomes hardly inhibited the growth of Gram(+)bacteria including the oral probiotics(e.g.,Limosilactobacillus reuteri).Overall,we envisage that the lysolecithin liposomes may serve as an excellent nano-scale delivery system of multi-functional free fatty acids to targets in complex food systems.展开更多
文摘In this research, Lysolecithin-a substance made with 100% natural ingredients - was given to ICR mice as medication to measure its periodic effect on the noradrenalin (NA), dopamine (DA), and serotonin (5-HT) levels of the brain. Both ICR and SAM mice were separated into two groups - control group and Lysolecithin (K. Lysolecithin: hydrolytic lysolecithin) medicated group, and given 1-week preparation period. The K. Lysolecithin group was given 500mg/kg of K. Lysolecithin at 0.2mL per dosage for 4 weeks, and the control group was given the same amount of dosage of water during the same period. NA, DA and 5-HT concentrations were measured from the blood before medication and 8 weeks / 12 weeks / 16 weeks after the first medication. For the SAM mice, 8 weeks after they were medicated with K .Lysolecithin, Morris Water Maze Test was conducted for 7 consecutive days and then the concentrations were measured by drawing blood from the heart. The K. Lysolecithin medicated group showed a tendency to have a statistically significant higher concentrations of 5-HT and NA in the blood. Also, periodic examination showed that the monoamine levels were highest in the 12th week and declined thereafter.
基金We all thank Kemin Industries(Zhuhai)Co.Ltd for supplying LPC and supporting many advices for the trial.The work was supported by key research and development Plan of Shandong Province(2016GSF115005)Technology Development Plan Project of Shandong Province(2014GHY115006).
文摘An 8-week trial was conducted to investigate graded levels of dietary LPC on growth,apparent digestibility(ADs)of nutrients and lipid metabolism in juvenile turbot Scophthalmus maximus L.Five experimental diets were formulated which contain 11%crude fat with graded amounts of LPC(0,1000,2500,4000,5500 mg/kg diet).The fish fed diet with 11%crude lipid and 0 LPC was used as the negative control group(C-),meanwhile the fish fed diet with 12%crude fat and 0 LPC was used as the positive control group(C+).Each diet was fed to triplicate groups of turbot(initial body weight of 41 g)for 56d.Weight gain rate and specific growth rate of the fish fed LPC supplemented diets were significantly higher than the control groups,and the growth performance of the C-group was significantly lower than the others.The viscerosomatic index(VSI)was significantly decreased and the gall bladder somatic index(GSI)was significantly increased by dietary LPC.Crude lipid contents of muscle were elevated by dietary LPC.Apparent digestibility of energy was decreased by dietary LPC,but protein and lipid were not affected.Both total cholesterol(T-CHO)and high density lipoprotein cholesterol(HDL-C)were up-regulated,meanwhile neither alanine aminotransferase(ALT)nor total protein(TP)were down-regulated by dietary LPC.Enzymes involved in lipid metabolism(total lipase,hepatic lipase,lipoprotein lipase,fatty acids synthase,and lipase)were all elevated by dietary LPC.In conclusion,the lipid requirements of turbot were decreased by dietary LPC and the lipid utilization coefficient was enhanced.Based on SAS NLIN regression,dietary 870.37 mg/kg LPC was appropriate for turbot juvenile.
基金supported by National Research Foundation of Korea(NRF)(Project No.RS-2023-00218150)by Ottogi Ham Taiho Foundation(Project No.2022-013).
文摘Porphyromonas gingivalis is a notorious keystone periodontal pathogen.However,it is difficult to selectively inhibit its growth in the oral cavity.Thereby,liposome-based antibacterial agents were investigated to suppress the growth of P.gingivalis.The antibacterial activities of enzymatically prepared lysolecithin-fatty acid liposomes(Lyso-FAL)were comparable to those of free-form linoleic acid in a simple aqueous system with a minimum inhibitory concentration(MIC)of 4.8μg/mL.However,this value was 2.8-fold lower than those of the lecithin liposomes including the same amount of free fatty acids(e.g.,linoleic acid).This indicated that the lysolecithin liposomes are very efficient in the delivery of free fatty acids from the aqueous medium to P.gingivalis.Remarkably,the antibacterial activities in the presence of milk and milk alternatives were significantly stronger(MIC,35μg/mL)than free-form linoleic acid(MIC,≥100μg/mL).The higher antibacterial activities of the liposomal fatty acids might be due to the efficient delivery of the antibacterial fatty acids from the liposomes to P.gingivalis even in the presence of emulsified fat globules and protein micelles in milk and milk substitutes.Besides,the lysolecithin-free fatty acid liposomes hardly inhibited the growth of Gram(+)bacteria including the oral probiotics(e.g.,Limosilactobacillus reuteri).Overall,we envisage that the lysolecithin liposomes may serve as an excellent nano-scale delivery system of multi-functional free fatty acids to targets in complex food systems.
