AIM To investigate the material basis and mechanism underlying the therapeutic effect of DLC in T2DM.METHODS T2DM was triggered in rats using a high-sugar,high-fat diet alongside 35 mg/kg streptozotocin.The effect of ...AIM To investigate the material basis and mechanism underlying the therapeutic effect of DLC in T2DM.METHODS T2DM was triggered in rats using a high-sugar,high-fat diet alongside 35 mg/kg streptozotocin.The effect of DLC on the intestinal microbiota in T2DM rats was analyzed via 16S rDNA sequencing.Targeted metabolomics was conducted to evaluate the impact of DLC on the levels of nine short-chain fatty acids(SCFAs).Untargeted metabolomics examined DLC-induced alterations in fecal metabolites and associated metabolic pathways.Additionally,Spearman’s correlation analysis assessed gut microbiota and fecal metabolite relationships.RESULTS DLC significantly attenuated pathological weight loss,reduced fasting blood glucose levels,restored blood sugar homeostasis,and ameliorated dyslipidemia in T2DM rats.The 16S rDNA sequencing revealed that DLC enhanced microbial diversity and reversed intestinal dysbiosis.Targeted metabolomics indicated decreased acetic acid and propionic acid levels and increased butyric acid,isobutyric acid,and 2-methylbutyric acid levels after DLC treatment.Untargeted metabolomics revealed 57 metabolites with altered expression associated with amino acid,carbohydrate,purine,and biotin pathways.The Spearman analysis demonstrated significant links between specific gut microbiota taxa and fecal metabolites.CONCLUSION DLC may exert hypoglycemic effects by modulating intestinal flora genera,SCFA levels,and fecal metabolites.展开更多
Aiming at the problem that longan trees in Guangdong Province have long been affected by pests and diseases,and to address issues such as low efficiency,high cost,and limited coverage in longan pest and disease inspec...Aiming at the problem that longan trees in Guangdong Province have long been affected by pests and diseases,and to address issues such as low efficiency,high cost,and limited coverage in longan pest and disease inspection,this paper designs a drone-based AI inspection system for longan pests and diseases.The system uses drones as a platform to collect images of longan orchards,which are transmitted in real time via 4G/5G networks.Meanwhile,it integrates an AI algorithm model for AI early warning and prescription suggestions.In practical applications,the system can quickly locate the areas where pests and diseases occur,identify longan pests and diseases,and provide fruit farmers with a basis for timely prevention and control.It significantly enhances the timeliness and accuracy of longan pest and disease control,and offers strong technical support for the precise management of the longan industry.展开更多
本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信...本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信号肽、亚细胞定位、亲疏水性、二级结构、三级结构等进行预测。结果表明:成功克隆获得一条长度为1 098 bp的Dlf3h基因,由366个氨基酸组成;该基因在根中表达量最高,其次为叶,在茎中表达量最低;生物信息学分析表明Dlf3h没有信号肽和跨膜结构,是一种可溶性亲水蛋白,定位于细胞核内;二级结构元件主要由α-螺旋和无规则卷曲组成,具有卷曲螺旋;Ramachandran评估表明应用SWISS-MODEL构建的三级结构模型可靠,其配体结合位点为217 His和275 His。本研究结果为进一步研究龙眼类黄酮合成的分子调控机制提供依据。展开更多
[Objective] The aim was to screen longan varieties suitable for local production in Vietnam.[Method] The fruit botanical characteristics and quality characteristics of longan varieties introduced into Northern Mountai...[Objective] The aim was to screen longan varieties suitable for local production in Vietnam.[Method] The fruit botanical characteristics and quality characteristics of longan varieties introduced into Northern Mountainous Agriculture and Forestry Science Institute of Vietnam Academy of Agriculture Sciences were compared and analyzed.[Result] Two varieties with good general qualities including PMP6 and PHS1-1 which were suitable to be cultivated in the local area were selected.In addition,Phu Dong was the available processed germplasm resource.[Conclusion] This research will provide reference for longan production and breeding in Northern Mountainous.展开更多
[Objective] This study was conducted to investigate the fresh eating-quality of Hainan Iongan. [Methed] The quality analysis was conducted on on-season and off-season Iongan, which are main cultivars in Hainan consist...[Objective] This study was conducted to investigate the fresh eating-quality of Hainan Iongan. [Methed] The quality analysis was conducted on on-season and off-season Iongan, which are main cultivars in Hainan consistent in maturity. In order to understand the quality characteristics of Hainan fresh Iongan, saccharide contents, vitamin C content, edible rate, TSS, weight of single fruit, weight per fruit cluster and pesticide residues were detected in this study. [Result] There were no significant differences in quality between on-season and off-season Iongan, and the content of TSS in off-season fruit was slightly higher than that in on-season Iongan. The two main cultivars ‘Shixia＇ and ＇Chuliang＇ in Hainan differed significantly in edible quality. The weight of single fruit and edible rate of ‘Chuliang＇ were slightly higher than those of ‘Shixia＇ Iongan, and their sucrose contents were nearly equivalent. Vitamin C and TSS contents in ‘Shixia＇ Ionganwere higher than those in ‘Chuliang＇.‘Shixia＇ had a monosaccharide content significantly higher than ‘Chuliang＇, while its sucrose content was lower than ‘Chuliang＇. Only low contents of residual cypermethrin and diflubenzuron were detected in pericarp, and the contents of the 9 pesticides in fruit flesh were all lower than their detection limits. [Conclusion] Longan fruit produced in Hainan could all be eaten safely.展开更多
[Objective] The study aimed to provide supports for developing chilling and freezing injury monitoring and disaster damage assessment of longan(Dimocarpus Longan Lour.).[Method] Based on field observation data,the rel...[Objective] The study aimed to provide supports for developing chilling and freezing injury monitoring and disaster damage assessment of longan(Dimocarpus Longan Lour.).[Method] Based on field observation data,the relationships between longan canopy temperature and air temperature under different weather types(sunny,cloudy to sunny,cloudy,rainy,radiation chilling injury and advection chilling injury)in 2007-2008 winter were analyzed.[Result] Diurnal variations of longan canopy temperature under sunny and radiation chilling injury weather conditions were most dramatic,followed with those under cloudy to sunny condition,while variations under cloudy,rainy and advection chilling injury conditions were mild.Diurnal variations of orchard air temperature were also closely related to weather types.By using linear and curvilinear regression methods,the relationship models between longan canopy temperature and observation station air temperature were established.The models for cloudy,rainy and advection chilling injury had better effects than those for sunny,cloudy to sunny and radiation chilling injury;the models for night were better than those for daytime and the whole day.[Conclusion] To some extent,applying the relationship models between longan canopy temperature and observation station air temperature could make up the shortcoming of meteorological data which were higher than the real values.展开更多
基金Supported by the National Natural Science Foundation of China,No.82160771NATCM's Project of High-Level Construction of Key TCM Disciplines:Traditional Medicine of Chinese Minority(Zhuang Medicine),No.zyyzdxk-2023165+7 种基金Guangxi One Thousand Young and Middle-Aged College and University Backbones Teachers Cultivation Program,No.[2019]5Guangxi Traditional Chinese Medicine Multidisciplinary Cross Innovation Team Project,No.GZKJ2309Guangxi Key R&D Plan Project,No.AB21196016Guangxi Key Discipline of Traditional Chinese Medicine Zhuang Pharmacy,No.GZXK-Z-20-64The First-Class Subject of Traditional Chinese Medicine(Ethnic Pharmacy)in Guangxi,No.[2018]12Guangxi Science and Technology Base and Talent Special Project,No.AD20238058 and No.AD21238031the Third Batch of Cultivating High-level Talent Teams in the“Qi Huang Project”of the Guangxi University of Chinese Medicine,No.202406and Huang Danian Style Teacher Team From Universities in Guangxi Zhuang Autonomous Region“Traditional Chinese Medicine Inheritance and Innovation Teacher Team”,No.[2023]31.
文摘AIM To investigate the material basis and mechanism underlying the therapeutic effect of DLC in T2DM.METHODS T2DM was triggered in rats using a high-sugar,high-fat diet alongside 35 mg/kg streptozotocin.The effect of DLC on the intestinal microbiota in T2DM rats was analyzed via 16S rDNA sequencing.Targeted metabolomics was conducted to evaluate the impact of DLC on the levels of nine short-chain fatty acids(SCFAs).Untargeted metabolomics examined DLC-induced alterations in fecal metabolites and associated metabolic pathways.Additionally,Spearman’s correlation analysis assessed gut microbiota and fecal metabolite relationships.RESULTS DLC significantly attenuated pathological weight loss,reduced fasting blood glucose levels,restored blood sugar homeostasis,and ameliorated dyslipidemia in T2DM rats.The 16S rDNA sequencing revealed that DLC enhanced microbial diversity and reversed intestinal dysbiosis.Targeted metabolomics indicated decreased acetic acid and propionic acid levels and increased butyric acid,isobutyric acid,and 2-methylbutyric acid levels after DLC treatment.Untargeted metabolomics revealed 57 metabolites with altered expression associated with amino acid,carbohydrate,purine,and biotin pathways.The Spearman analysis demonstrated significant links between specific gut microbiota taxa and fecal metabolites.CONCLUSION DLC may exert hypoglycemic effects by modulating intestinal flora genera,SCFA levels,and fecal metabolites.
文摘Aiming at the problem that longan trees in Guangdong Province have long been affected by pests and diseases,and to address issues such as low efficiency,high cost,and limited coverage in longan pest and disease inspection,this paper designs a drone-based AI inspection system for longan pests and diseases.The system uses drones as a platform to collect images of longan orchards,which are transmitted in real time via 4G/5G networks.Meanwhile,it integrates an AI algorithm model for AI early warning and prescription suggestions.In practical applications,the system can quickly locate the areas where pests and diseases occur,identify longan pests and diseases,and provide fruit farmers with a basis for timely prevention and control.It significantly enhances the timeliness and accuracy of longan pest and disease control,and offers strong technical support for the precise management of the longan industry.
文摘本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信号肽、亚细胞定位、亲疏水性、二级结构、三级结构等进行预测。结果表明:成功克隆获得一条长度为1 098 bp的Dlf3h基因,由366个氨基酸组成;该基因在根中表达量最高,其次为叶,在茎中表达量最低;生物信息学分析表明Dlf3h没有信号肽和跨膜结构,是一种可溶性亲水蛋白,定位于细胞核内;二级结构元件主要由α-螺旋和无规则卷曲组成,具有卷曲螺旋;Ramachandran评估表明应用SWISS-MODEL构建的三级结构模型可靠,其配体结合位点为217 His和275 His。本研究结果为进一步研究龙眼类黄酮合成的分子调控机制提供依据。
基金Supported by Introduction of Overseas Tropic Crops Germplasm Resources(948Program of China)(2010-G2-08)a Program for Construction of Modern Agriculture Industrial Technology System(nycytx-32-17)~~
文摘[Objective] The aim was to screen longan varieties suitable for local production in Vietnam.[Method] The fruit botanical characteristics and quality characteristics of longan varieties introduced into Northern Mountainous Agriculture and Forestry Science Institute of Vietnam Academy of Agriculture Sciences were compared and analyzed.[Result] Two varieties with good general qualities including PMP6 and PHS1-1 which were suitable to be cultivated in the local area were selected.In addition,Phu Dong was the available processed germplasm resource.[Conclusion] This research will provide reference for longan production and breeding in Northern Mountainous.
基金Supported by National Science&Technology Infrastructure-National Infrastructure of Agriculture Germplasm Resources"National infrastructure of tropical crops germplasm resources/NICGR2016-067"China Agriculture Research System(CARS-33-25)~~
文摘[Objective] This study was conducted to investigate the fresh eating-quality of Hainan Iongan. [Methed] The quality analysis was conducted on on-season and off-season Iongan, which are main cultivars in Hainan consistent in maturity. In order to understand the quality characteristics of Hainan fresh Iongan, saccharide contents, vitamin C content, edible rate, TSS, weight of single fruit, weight per fruit cluster and pesticide residues were detected in this study. [Result] There were no significant differences in quality between on-season and off-season Iongan, and the content of TSS in off-season fruit was slightly higher than that in on-season Iongan. The two main cultivars ‘Shixia＇ and ＇Chuliang＇ in Hainan differed significantly in edible quality. The weight of single fruit and edible rate of ‘Chuliang＇ were slightly higher than those of ‘Shixia＇ Iongan, and their sucrose contents were nearly equivalent. Vitamin C and TSS contents in ‘Shixia＇ Ionganwere higher than those in ‘Chuliang＇.‘Shixia＇ had a monosaccharide content significantly higher than ‘Chuliang＇, while its sucrose content was lower than ‘Chuliang＇. Only low contents of residual cypermethrin and diflubenzuron were detected in pericarp, and the contents of the 9 pesticides in fruit flesh were all lower than their detection limits. [Conclusion] Longan fruit produced in Hainan could all be eaten safely.
基金Supported by National Key Project of Scientific and Technical Support-ing Programs Funded by Ministry of Science & Technology of China during the11thFive-Year Plan Period"Study on Monitoring,Early Warning and Control Techniques of Major Agricultural Meteorological Disasters--Study on Monitoring and Early Warning Techniques of Chilling Injury in South China"(2006BAD04B03)Subject of National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Tech-nology of China"Study on Chilling and Freezing Injuries Assessment,Mo-nitoring and Warning Techniques of Main Subtropical Crops"(2008BADB8B01)~~
文摘[Objective] The study aimed to provide supports for developing chilling and freezing injury monitoring and disaster damage assessment of longan(Dimocarpus Longan Lour.).[Method] Based on field observation data,the relationships between longan canopy temperature and air temperature under different weather types(sunny,cloudy to sunny,cloudy,rainy,radiation chilling injury and advection chilling injury)in 2007-2008 winter were analyzed.[Result] Diurnal variations of longan canopy temperature under sunny and radiation chilling injury weather conditions were most dramatic,followed with those under cloudy to sunny condition,while variations under cloudy,rainy and advection chilling injury conditions were mild.Diurnal variations of orchard air temperature were also closely related to weather types.By using linear and curvilinear regression methods,the relationship models between longan canopy temperature and observation station air temperature were established.The models for cloudy,rainy and advection chilling injury had better effects than those for sunny,cloudy to sunny and radiation chilling injury;the models for night were better than those for daytime and the whole day.[Conclusion] To some extent,applying the relationship models between longan canopy temperature and observation station air temperature could make up the shortcoming of meteorological data which were higher than the real values.
