The hormone defined serum free conditioned medium (SFCM) of human nasopharyngeal carcinoma epithelioid cell line (CNE1) was assayed by both the 3H-thymidine incorporation test and the soft agar test. It was found that...The hormone defined serum free conditioned medium (SFCM) of human nasopharyngeal carcinoma epithelioid cell line (CNE1) was assayed by both the 3H-thymidine incorporation test and the soft agar test. It was found that the SFCM stimulated the growth of long-term serum-free cultured CNE4 cells in ac-cordence with the fact that the growth rate of long-term serum-free cultured CNE1 cells was directly proportional to the plating density. Alternatively 5% SFCM inhibited the growth of short-term serum-free cultured CNE4 cells by 51% in which the indicator cell remained the responsiveness state of growing in the serum-supplemented medium to the effector of interest. Furthermore, SFCM resulted in the inhibition of anchorage-independent growth of CNE4 cells and A431 cells. Also in soft agar test. SFCM reduced the colony formation of NRK(?),9F cells in the presence of EGF or EGF plus TGF-β. These finding suggested that CNE4 secreted autocrine growth stimulating factor(s) and growth inhibiting factor(s) in the serum-free medium, the latter strongly reverse malignant phenotypes of CNE4 and A431 cells in serum-supplemented surrounding.展开更多
The near crack line field analysis method has been used io investigate into theexact elastic-plastic solutions of a mode II crack under plane stress condilion in anelastic-perfectly plastic solid. The assumptions of t...The near crack line field analysis method has been used io investigate into theexact elastic-plastic solutions of a mode II crack under plane stress condilion in anelastic-perfectly plastic solid. The assumptions of the usual small scale yielding theory.hare been completely. dbandoned and the correct formulations of matching conditionsat the elaslic-plastic boundary. have been given. By, matching the general solution ofthe plastic slress field (bul not the special solution used to be adopted) with the exactelastic stress field (but not the crack tip K-dominant field) at the elastic-plasticboundary, near the crack line, the plastic stresses. the length of the plastic =one and theunit normal vector of the elastic-plastic boundary.which are sufficiently precise near the crack line region ,have been given.展开更多
The near crack line field analysis method has been used to investigate into the exact elastic-plastic solutions of a mode Ⅱ crack under plane stress condition in anelastic-perfectly plastic solid. The assumptions of ...The near crack line field analysis method has been used to investigate into the exact elastic-plastic solutions of a mode Ⅱ crack under plane stress condition in anelastic-perfectly plastic solid. The assumptions of the usual small scale yielding theoryhave been completely. dbandoned and the correct .formulations of matching conditionsat the elastic-plastic boundary have been given. By matching the general solution of the plastic stress field (but not the special solution used to be adopted) will the exactelastic stress field (but not the crack tip K-dominant field) at the elastic-plasticboundary. near the crack line, the plastic .stresses, the length of the plastic zone and theunit normal vector of the elaslic-plastic boundary. which sufficiently precise nearthe crack line region, hare been given.展开更多
文摘The hormone defined serum free conditioned medium (SFCM) of human nasopharyngeal carcinoma epithelioid cell line (CNE1) was assayed by both the 3H-thymidine incorporation test and the soft agar test. It was found that the SFCM stimulated the growth of long-term serum-free cultured CNE4 cells in ac-cordence with the fact that the growth rate of long-term serum-free cultured CNE1 cells was directly proportional to the plating density. Alternatively 5% SFCM inhibited the growth of short-term serum-free cultured CNE4 cells by 51% in which the indicator cell remained the responsiveness state of growing in the serum-supplemented medium to the effector of interest. Furthermore, SFCM resulted in the inhibition of anchorage-independent growth of CNE4 cells and A431 cells. Also in soft agar test. SFCM reduced the colony formation of NRK(?),9F cells in the presence of EGF or EGF plus TGF-β. These finding suggested that CNE4 secreted autocrine growth stimulating factor(s) and growth inhibiting factor(s) in the serum-free medium, the latter strongly reverse malignant phenotypes of CNE4 and A431 cells in serum-supplemented surrounding.
文摘The near crack line field analysis method has been used io investigate into theexact elastic-plastic solutions of a mode II crack under plane stress condilion in anelastic-perfectly plastic solid. The assumptions of the usual small scale yielding theory.hare been completely. dbandoned and the correct formulations of matching conditionsat the elaslic-plastic boundary. have been given. By, matching the general solution ofthe plastic slress field (bul not the special solution used to be adopted) with the exactelastic stress field (but not the crack tip K-dominant field) at the elastic-plasticboundary, near the crack line, the plastic stresses. the length of the plastic =one and theunit normal vector of the elastic-plastic boundary.which are sufficiently precise near the crack line region ,have been given.
文摘The near crack line field analysis method has been used to investigate into the exact elastic-plastic solutions of a mode Ⅱ crack under plane stress condition in anelastic-perfectly plastic solid. The assumptions of the usual small scale yielding theoryhave been completely. dbandoned and the correct .formulations of matching conditionsat the elastic-plastic boundary have been given. By matching the general solution of the plastic stress field (but not the special solution used to be adopted) will the exactelastic stress field (but not the crack tip K-dominant field) at the elastic-plasticboundary. near the crack line, the plastic .stresses, the length of the plastic zone and theunit normal vector of the elaslic-plastic boundary. which sufficiently precise nearthe crack line region, hare been given.
