为探究L-瓜氨酸(L-Cit)与来曲唑(LE)对湖羊母羊繁殖性能的影响,本试验选取体重(51.57±2.64)kg、健康的湖羊经产母羊60只,随机分为3个处理组,分别为CON组、L-Cit组和L-Cit+LE组,每组20只羊,每日摄入2.2 kg TMR。在此日粮基础上,L-Ci...为探究L-瓜氨酸(L-Cit)与来曲唑(LE)对湖羊母羊繁殖性能的影响,本试验选取体重(51.57±2.64)kg、健康的湖羊经产母羊60只,随机分为3个处理组,分别为CON组、L-Cit组和L-Cit+LE组,每组20只羊,每日摄入2.2 kg TMR。在此日粮基础上,L-Cit组每只母羊每天一次性补充10 g L-Cit,L-Cit+LE组在每只母羊补饲10 g/d L-Cit基础上,于试验第7~9天每只母羊每天补饲LE 2.5 mg,进行为期21 d的试验。结果表明:(1)补饲L-瓜氨酸至第7天时,L-Cit组和L-Cit+LE组与对照组相比促性腺激素释放激素(GnRH)含量分别提高了11.11%、15.23%(P<0.01),促卵泡素(FSH)含量分别降低了9.19%、23.68%(P<0.01),促黄体激素(LH)含量分别降低了11.54%、18.95%(P=0.06),L-Cit+LE组较CON组孕酮(P4)含量提高了145.45%(P<0.05),雌二醇(E2)、睾酮(T)含量均无显著差异(P>0.05)。(2)试验第9天时,L-Cit组和L-Cit+LE组与对照组相比GnRH含量分别提高了11.22%、17.45%(P<0.01),E2含量分别降低了46.37%、45.01%(P<0.01)。(3)试验第14天时,L-Cit组和L-Cit+LE组与对照组相比GnRH含量分别提高了5.69%、16.13%(P<0.01),FSH含量分别降低了19.95%、32.63%(P<0.05),L-Cit+LE组与其他两组相比LH含量降低了26.35%、19.97%(P<0.01)。(4)L-Cit组双羔率较L-Cit+LE组提高了35.82%,双羔出生重较对照组提高了0.12 kg。综上所述,在本试验条件下,补喂10 g/d·只的L-瓜氨酸可极显著提高经产湖羊母羊的发情率、产羔数和血浆中GnRH含量。展开更多
The considerable post-harvest losses (5% to 40% depending on storage time) due to the high water content level of the onion varieties in Senegal are the main cause of the problem of access to local onion all year roun...The considerable post-harvest losses (5% to 40% depending on storage time) due to the high water content level of the onion varieties in Senegal are the main cause of the problem of access to local onion all year round. Therefore, drying is one of the techniques that can be used to solve the problem of onion perishability. This study deals with the characterization of naturally convective kinetics drying of four onion varieties in relation to their maturity level. The experiment was carried out using the gravimetric method. The Welch and Turkey statistical tests display a significant difference between the effective diffusivity coefficients depending on the maturity level within each variety and across the four varieties. The effective diffusivity coefficients of the Galmi Violet, Safari, Gandiol F1 and Orient F1varieties range from 2.18 × 10−11 ± 2.69 × 10−12 to 1.32 × 10−10 ± 1.17 × 10−11 m2⋅s−1 at a maturity level less than 80%. When the maturity level is greater than 85%, the effective diffusivity coefficients range from 1.30 × 10−11 ± 1.24 × 10−12 to 8.05 × 10−11 ± 8.94 × 10−13 m2⋅s−1. As far as the activation energy is concerned, the study only reveals a significant difference between the varieties whatever the maturity level is. The Galmi Violet variety stands out with an average activation energy of 66.71 ± 0.12 KJ⋅mol−1 K−1 for the maturity level below 80% and 58.74 ± 0.11 KJ⋅mol−1 for the maturity level above 85%. For the three remaining varieties, the average activation energy ranges from 58.15 ± 0.19 to 59.12 ± 0.13 KJ⋅mol−1 for a maturity level less than 80% whereas the rates go from 47.63 ± 0.28 to 49.96 ± 0.77 KJ⋅mol−1 when the maturity level is greater than 85%. In summary, the higher the maturity level is, the lower the effective diffusivity coefficients will be. The same tendency was observed with the activation energy. The Galmi Violet variety represents the limitative one in case of the drying of the four varieties mix together.展开更多
This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two C...This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two CO2 levels (360 and 1000 μl/L) and three Cd levels (0,4,and 16 mg/L) under hydroponic conditions.The results show that elevated levels of CO2 increased shoot biomass more,compared to root biomass,but decreased Cd concentrations in all plant tissues.Cd exposure caused toxicity to both Lolium species,as shown by the restrictions of the root morphological parameters including root length,surface area,volume,and tip numbers.These parameters were significantly higher under elevated levels of CO2 than under ambient CO2,especially for the number of fine roots.The increases in magnitudes of those parameters triggered by elevated levels of CO2 under Cd stress were more than those under non-Cd stress,suggesting an ameliorated Cd stress under elevated levels of CO2.The total Cd uptake per pot,calculated on the basis of biomass,was significantly greater under elevated levels of CO2 than under ambient CO2.Ameliorated Cd toxicity,decreased Cd concentration,and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation.展开更多
Somatic embryogenesis （SE） is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE （SERK） gene is known to function...Somatic embryogenesis （SE） is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE （SERK） gene is known to function in SE. A homolog GhSERK2 （accession number： JF430801） was cloned from Upland cotton and characterized for its functions in SE. GhSERK2 expressed in different tissues and showed higher expression level in floral organs than vegetative ones with the highest levels in ovule and anther. GhSERK2 expressed during SE with a high level at globular embryos stage. Upon treatment with indole-3-butytic acid （IBA）, the transcription level of GhSERK2 was induced and promoted SE subsequently. A 2-day treatment of 2,4-dichlorophenoxyacetic acid （2,4-D） induced the expression of GhSERK2, but treatments of 2,4-D for longer periods sharply inhibited the GhSERK2 transcription level of embryogenic callus （EC）. The levels of hormones, including 3-indoleacetic acid （IAA）, abscisic acid （ABA）, and brassinosteroid （BR）, were increased in the initial calli induced from the over-expression of GhSERK2 cotton. Our results indicated that GhSERK2 expression was associated with induction of SE and closely related to hormone levels during tissue culture in Upland cotton, and the gene might play an important role in regeneration of cotton.展开更多
The investigation was carried out to study the response of winter rapeseed to potassium （K） feritlization and the critical soil available K level for current winter rapeseed production in the Yangtze River Valley （...The investigation was carried out to study the response of winter rapeseed to potassium （K） feritlization and the critical soil available K level for current winter rapeseed production in the Yangtze River Valley （YRV） of China. A total of 132 field experiments were conducted in fields of farmers in the major winter rapeseed growing areas in YRV in 2000/2001 and 2004/2005 to 2006/2007 during growing season. Results of these field experiments showed that the average rapeseed yield increment resulting from 100 kg K ha-1 application was 358 kg ha-1, an increase over the control CK （no K） of 18.0% in 2005/2006 and 2006/2007. The average internal use efficiency （IE） of K was higher in the CK treatment （21.9 kg grain, kg-1 K uptake） than in the ＋K （100 kg K ha-1） treatment （17.7 kg grain, kg-1 K uptake）. Winter rapeseed required 68.1 kg of K to produce 1 000 kg seed. The recovery efficiency of K fertilizer in rapeseed production averaged 39.3%. The K balance was negative, with an average net removal of 117.6 kg K ha-1 in the CK treatment annually, and 56.8 kg K ha-1 in the ＋K treatment. The results indicated that there was a significant negative relationship between yield increments by K application and soil available K content. Based on the relative yield of CK/＋K at 90% level, the critical level of soil available K （NH4OAc-extractable K） was 135 mg kg-1.展开更多
Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragment...Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes.展开更多
文摘The considerable post-harvest losses (5% to 40% depending on storage time) due to the high water content level of the onion varieties in Senegal are the main cause of the problem of access to local onion all year round. Therefore, drying is one of the techniques that can be used to solve the problem of onion perishability. This study deals with the characterization of naturally convective kinetics drying of four onion varieties in relation to their maturity level. The experiment was carried out using the gravimetric method. The Welch and Turkey statistical tests display a significant difference between the effective diffusivity coefficients depending on the maturity level within each variety and across the four varieties. The effective diffusivity coefficients of the Galmi Violet, Safari, Gandiol F1 and Orient F1varieties range from 2.18 × 10−11 ± 2.69 × 10−12 to 1.32 × 10−10 ± 1.17 × 10−11 m2⋅s−1 at a maturity level less than 80%. When the maturity level is greater than 85%, the effective diffusivity coefficients range from 1.30 × 10−11 ± 1.24 × 10−12 to 8.05 × 10−11 ± 8.94 × 10−13 m2⋅s−1. As far as the activation energy is concerned, the study only reveals a significant difference between the varieties whatever the maturity level is. The Galmi Violet variety stands out with an average activation energy of 66.71 ± 0.12 KJ⋅mol−1 K−1 for the maturity level below 80% and 58.74 ± 0.11 KJ⋅mol−1 for the maturity level above 85%. For the three remaining varieties, the average activation energy ranges from 58.15 ± 0.19 to 59.12 ± 0.13 KJ⋅mol−1 for a maturity level less than 80% whereas the rates go from 47.63 ± 0.28 to 49.96 ± 0.77 KJ⋅mol−1 when the maturity level is greater than 85%. In summary, the higher the maturity level is, the lower the effective diffusivity coefficients will be. The same tendency was observed with the activation energy. The Galmi Violet variety represents the limitative one in case of the drying of the four varieties mix together.
基金Project supported by the Central Public Research Institute Basic Fund for Research and Development (2008-jxh-1),Agro-environmental Protection Institute,Ministry of Agriculture,China
文摘This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two CO2 levels (360 and 1000 μl/L) and three Cd levels (0,4,and 16 mg/L) under hydroponic conditions.The results show that elevated levels of CO2 increased shoot biomass more,compared to root biomass,but decreased Cd concentrations in all plant tissues.Cd exposure caused toxicity to both Lolium species,as shown by the restrictions of the root morphological parameters including root length,surface area,volume,and tip numbers.These parameters were significantly higher under elevated levels of CO2 than under ambient CO2,especially for the number of fine roots.The increases in magnitudes of those parameters triggered by elevated levels of CO2 under Cd stress were more than those under non-Cd stress,suggesting an ameliorated Cd stress under elevated levels of CO2.The total Cd uptake per pot,calculated on the basis of biomass,was significantly greater under elevated levels of CO2 than under ambient CO2.Ameliorated Cd toxicity,decreased Cd concentration,and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation.
基金supported in part by the National Natural Science Foundation of China (31371666)a grant from the National Key Specific Program to Hua Jinping (2016ZX08005-003)
文摘Somatic embryogenesis （SE） is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE （SERK） gene is known to function in SE. A homolog GhSERK2 （accession number： JF430801） was cloned from Upland cotton and characterized for its functions in SE. GhSERK2 expressed in different tissues and showed higher expression level in floral organs than vegetative ones with the highest levels in ovule and anther. GhSERK2 expressed during SE with a high level at globular embryos stage. Upon treatment with indole-3-butytic acid （IBA）, the transcription level of GhSERK2 was induced and promoted SE subsequently. A 2-day treatment of 2,4-dichlorophenoxyacetic acid （2,4-D） induced the expression of GhSERK2, but treatments of 2,4-D for longer periods sharply inhibited the GhSERK2 transcription level of embryogenic callus （EC）. The levels of hormones, including 3-indoleacetic acid （IAA）, abscisic acid （ABA）, and brassinosteroid （BR）, were increased in the initial calli induced from the over-expression of GhSERK2 cotton. Our results indicated that GhSERK2 expression was associated with induction of SE and closely related to hormone levels during tissue culture in Upland cotton, and the gene might play an important role in regeneration of cotton.
基金funded by the National Key Technologies R&D Program during the 11th Five-Year Plan period of China (2008BADA4B08)the Program for New Century Excellent Talents in University of Ministry of Education of China (NCET-07-0345)+2 种基金the Earmarked Fund for Modern Agro-Industry Technology Research System of Ministry of Agriculture of China (nycytx-005)the collaborative project between China and the International Plant Nutrition Institute (IPNI)between China and the International Potash Institute (IPI)
文摘The investigation was carried out to study the response of winter rapeseed to potassium （K） feritlization and the critical soil available K level for current winter rapeseed production in the Yangtze River Valley （YRV） of China. A total of 132 field experiments were conducted in fields of farmers in the major winter rapeseed growing areas in YRV in 2000/2001 and 2004/2005 to 2006/2007 during growing season. Results of these field experiments showed that the average rapeseed yield increment resulting from 100 kg K ha-1 application was 358 kg ha-1, an increase over the control CK （no K） of 18.0% in 2005/2006 and 2006/2007. The average internal use efficiency （IE） of K was higher in the CK treatment （21.9 kg grain, kg-1 K uptake） than in the ＋K （100 kg K ha-1） treatment （17.7 kg grain, kg-1 K uptake）. Winter rapeseed required 68.1 kg of K to produce 1 000 kg seed. The recovery efficiency of K fertilizer in rapeseed production averaged 39.3%. The K balance was negative, with an average net removal of 117.6 kg K ha-1 in the CK treatment annually, and 56.8 kg K ha-1 in the ＋K treatment. The results indicated that there was a significant negative relationship between yield increments by K application and soil available K content. Based on the relative yield of CK/＋K at 90% level, the critical level of soil available K （NH4OAc-extractable K） was 135 mg kg-1.
基金Foundation： Special program for public health of traditional Chinese medicine （Fiscal agency [ 2011 ] No.76） Special program for Chinese pharmaceutical industry （No.201207002）
基金Supported by Natural Science Foundation of Yunnan Province(2011FB049)National Natural Science Foundation of China(31260481,31460516)+2 种基金Fund of Yunnan Education Department(2013Y251)Fund of the Department of Life Science and Technology,Kunming University(GXKM201505)Talent Fund for PhD(YJL11015)
文摘Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes.
