Seasonal dynamics of above- and belowground biomass and nutrient characteristics （nitrogen, carbon, and phosphorus） of Carex lasiocarpa were investigated in the typical wetland of Sanjiang Plain, China from May 2007...Seasonal dynamics of above- and belowground biomass and nutrient characteristics （nitrogen, carbon, and phosphorus） of Carex lasiocarpa were investigated in the typical wetland of Sanjiang Plain, China from May 2007 to September 2008. The results show that the changes of aboveground biomass during the growing season are best described by the twice function curve model, whereas the changes of belowground biomass follow the exponential increase curve model. Both the organic carbon contents in the above- and belowground plant parts show significant positive linear correlations with the growing time, and the coefficients R2 are 0.983 and 0.746, respectively. The carbon accu-mulations of the above- and belowground plant parts during the growing season show the same dynamics as those of the biomass. However, the nitrogen contents and accumulation in C. lasiocarpa aboveground and belowground parts show exponential increase during the growing season. The dynamics of C. lasiocarpa phosphorus contents follows the twice function curve model, whereas the accumulation of phosphorus shows the linear increase. The ratios of C/N in different parts of C. lasiocarpa fit the negative linear relations with total nitrogen content in the growing season. Moreover, the ratios of C/P in C. lasiocarpa plant also fit the negative linear relations with total phosphorus content. The results show that nitrogen is the primary limiting nutrient for C. lasiocarpa growth as compared with carbon and phosphorus.展开更多
Phytochemical investigation of the aerial parts of the monotypic plant,Musella lasiocarpa,led to the isolation of four rare bicyclic diarylheptanoids,musellarins B-E(2-5),two new phenylphenalenones,2-methoxy-9-(3′,4...Phytochemical investigation of the aerial parts of the monotypic plant,Musella lasiocarpa,led to the isolation of four rare bicyclic diarylheptanoids,musellarins B-E(2-5),two new phenylphenalenones,2-methoxy-9-(3′,4′-dihydroxyphenyl)-1H-phenalen-1-one(9),2-methoxy-9-(3′-methoxy-4′-hydroxyphenyl)-1H-phenalen-1-one(10),a new acenaphtylene derivative,trans-(1S,2S)-3-(4′-methoxyphenyl)-acenaphthene-1,2-diol(13),and two new sucrose esters,1,2′,3′,4′,6′-O-pentaacetyl-3-O-trans-p-coumaroylsucrose(16),1,2′,3′,4′,6′-O-pentaacetyl-3-O-cis-p-coumaroylsucrose(17),together with nine known compounds.In addition,(4E,6E)-1-(3′,4′-dihydroxyphenyl)-7-(4′′-hydroxyphenyl)-hepta-4,6-dien-3-one(15)was isolated for the first time from a natural source.The structures of new compounds were elucidated by analysis of their spectroscopic data.Compounds 2,6,8-10,12,and 14 were cytotoxic toward several of the human tumor cell lines(HL-60,SMMC-7721,A-549,MCF-7,and SW480).Of these,the new compound 9 was the most potent one,with IC50 values of 5.8,10.3,6.3,3.3,and 2.3μM,respectively.展开更多
The strength of phylogeographic breaks can vary among species in the same area despite being subject to the same geological and climate history due to differences in biological traits.Several important phylogeographic...The strength of phylogeographic breaks can vary among species in the same area despite being subject to the same geological and climate history due to differences in biological traits.Several important phylogeographic breaks exist around the Sichuan Basin in Southwest China but few studies have focused on wind-dispersed plants.Here,we investigated the phylogeographic patterns and the evolutionary history of Populus lasiocarpa,a wind-pollinated and wind-dispersed tree species with a circum-Sichuan Basin distribution in southwest China.We sequenced and analyzed three plastid DNA fragments(ptDNA)and eight nuclear microsatellites(nSSRs)of 265 individuals of P.lasiocarpa from 21 populations spanning the entire distribution range.Distribution patterns based on nSSR data revealed that there are three genetic groups in P.lasiocarpa.This is consistent with the three phylogeographic breaks(Sichuan Basin,the Kaiyong Line and the 105°E line),where the Sichuan basin acts as the main barrier to gene flow between western and eastern groups.However,the distribution pattern based on ptDNA haplotypes poorly matched the phylogeographic breaks,and wind-dispersed seeds may be one of the main contributing factors.Species distribution modelling suggested a larger potential distribution in the last glacial maximum with a severe bottleneck during the last interglacial.A DIYABC model also suggested a population contraction and expansion for both western and eastern lineages.These results indicate that biological traits are likely to affect the evolutionary history of plants,and that nuclear molecular markers,which experience higher levels of gene flow,might be better indicators of phylogeographic breaks.展开更多
In order to investigate the dew amounts and analysis formation conditions of dew by examining the correlations between dew amounts and meteorological factors in Carex lasiocarpa marsh in the Sanjiang Plain,the dew amo...In order to investigate the dew amounts and analysis formation conditions of dew by examining the correlations between dew amounts and meteorological factors in Carex lasiocarpa marsh in the Sanjiang Plain,the dew amounts and several meteorological factors were monitored during the growing season from June to September in 2005 and 2008 at the Sanjiang Mire Wetland Experimental Station,Chinese Academy of Sciences.Dew was collected by woodstick made by poplar tree in Carex lasiocarpa marsh and the amounts were estimated by subtraction method.The results indicated that average daily accumulated dew amounts reached the peak in August.The highest dew amounts in Carex lasiocarpa marsh were 0.201 mm/d,and the most frequent dew volume ranged from 0.08 mm/d to 0.14 mm/d.The amounts correlated positively with relative humidity,dew point temperature,and water vapor pressure.Whereas it correlated negatively with wind speed.The dew production in Carex lasiocarpa marsh more frequently occurred under the conditions of wind speed around 2.0 m/s,the water vapor pressure above 9 hPa;the relative humidity between 90% and 95% and dew point temperature above 6 ℃.展开更多
利用超高效液相色谱四极杆飞行时间串联质谱联用(UPLC-Q-TOF-MS)代谢组学技术,对地涌金莲[Musella lasiocarpa(Franch.) C. Y. Wu ex H. W. Li]等6种芭蕉亚科(subfam. Musoideae)植物种子代谢物进行非靶向分析,并进一步对地涌金莲不同...利用超高效液相色谱四极杆飞行时间串联质谱联用(UPLC-Q-TOF-MS)代谢组学技术,对地涌金莲[Musella lasiocarpa(Franch.) C. Y. Wu ex H. W. Li]等6种芭蕉亚科(subfam. Musoideae)植物种子代谢物进行非靶向分析,并进一步对地涌金莲不同成熟期种子及不同组织的代谢物进行靶向分析,还对7种化合物进行了体外抗真菌活性检测。结果显示:地涌金莲种子代谢物与芭蕉亚科另5种植物差异明显。地涌金莲与蕉麻(Musa textilis Née)之间的显著差异代谢物最少(279),与朝天蕉(Musa velutina H. Wendl. et Drude)之间的显著差异代谢物最多(429);地涌金莲与另5种植物的共有显著差异代谢物数量为63,识别鉴定出37个代谢物,其中,4个二芳基庚烷类化合物在地涌金莲种子中的相对含量显著高于另5种植物。靶向分析结果显示:在地涌金莲4个成熟期种子中共鉴定到12个二芳基庚烷类显著差异代谢物,且多数化合物在黑色或深褐色种子中的相对含量最高。对地涌金莲不同组织中的二芳基庚烷类化合物含量进行比较,结果显示这类化合物多集中在地下组织(假茎、假茎皮、根)。抗真菌活性实验结果显示:2-methoxy-9-phenyl-1H-phenalen-1-one对尖孢镰刀菌(Fusarium oxysporum)、胶孢炭疽菌(Colletotrichum gloeosporioides)和链格孢菌(Alternaria alternata)的抑制率显著(P<0.05)高于其他化合物。综合分析结果显示:地涌金莲富含二芳基庚烷类化合物,且多数二芳基庚烷类化合物为苯基非那烯酮类化合物,且具有较高的抗真菌活性。展开更多
基金This research was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (No.KZCX2–YW–309)the Major State Basic Research Development Program of China (973 Program No. 2004CB418507)
文摘Seasonal dynamics of above- and belowground biomass and nutrient characteristics （nitrogen, carbon, and phosphorus） of Carex lasiocarpa were investigated in the typical wetland of Sanjiang Plain, China from May 2007 to September 2008. The results show that the changes of aboveground biomass during the growing season are best described by the twice function curve model, whereas the changes of belowground biomass follow the exponential increase curve model. Both the organic carbon contents in the above- and belowground plant parts show significant positive linear correlations with the growing time, and the coefficients R2 are 0.983 and 0.746, respectively. The carbon accu-mulations of the above- and belowground plant parts during the growing season show the same dynamics as those of the biomass. However, the nitrogen contents and accumulation in C. lasiocarpa aboveground and belowground parts show exponential increase during the growing season. The dynamics of C. lasiocarpa phosphorus contents follows the twice function curve model, whereas the accumulation of phosphorus shows the linear increase. The ratios of C/N in different parts of C. lasiocarpa fit the negative linear relations with total nitrogen content in the growing season. Moreover, the ratios of C/P in C. lasiocarpa plant also fit the negative linear relations with total phosphorus content. The results show that nitrogen is the primary limiting nutrient for C. lasiocarpa growth as compared with carbon and phosphorus.
基金This work was financially supported by the National Basic Research Program of China(973 Program No.2009CB522303 and No.2011CB915503)the NSFC(No.U0932602)+1 种基金the National Natural Science Foundation of China(No.90813004)the State Key Laboratory of Phytochemistry and Plant Resources in West China(No.P2010-ZZ05).
文摘Phytochemical investigation of the aerial parts of the monotypic plant,Musella lasiocarpa,led to the isolation of four rare bicyclic diarylheptanoids,musellarins B-E(2-5),two new phenylphenalenones,2-methoxy-9-(3′,4′-dihydroxyphenyl)-1H-phenalen-1-one(9),2-methoxy-9-(3′-methoxy-4′-hydroxyphenyl)-1H-phenalen-1-one(10),a new acenaphtylene derivative,trans-(1S,2S)-3-(4′-methoxyphenyl)-acenaphthene-1,2-diol(13),and two new sucrose esters,1,2′,3′,4′,6′-O-pentaacetyl-3-O-trans-p-coumaroylsucrose(16),1,2′,3′,4′,6′-O-pentaacetyl-3-O-cis-p-coumaroylsucrose(17),together with nine known compounds.In addition,(4E,6E)-1-(3′,4′-dihydroxyphenyl)-7-(4′′-hydroxyphenyl)-hepta-4,6-dien-3-one(15)was isolated for the first time from a natural source.The structures of new compounds were elucidated by analysis of their spectroscopic data.Compounds 2,6,8-10,12,and 14 were cytotoxic toward several of the human tumor cell lines(HL-60,SMMC-7721,A-549,MCF-7,and SW480).Of these,the new compound 9 was the most potent one,with IC50 values of 5.8,10.3,6.3,3.3,and 2.3μM,respectively.
基金supported by National Natural Science Foundation of China(grants 31971567 and 31622015)Fundamental Research Funds for the Central Universities(YJ201936,SCU2020D003,SCU2021D006,SCU2022D003)。
文摘The strength of phylogeographic breaks can vary among species in the same area despite being subject to the same geological and climate history due to differences in biological traits.Several important phylogeographic breaks exist around the Sichuan Basin in Southwest China but few studies have focused on wind-dispersed plants.Here,we investigated the phylogeographic patterns and the evolutionary history of Populus lasiocarpa,a wind-pollinated and wind-dispersed tree species with a circum-Sichuan Basin distribution in southwest China.We sequenced and analyzed three plastid DNA fragments(ptDNA)and eight nuclear microsatellites(nSSRs)of 265 individuals of P.lasiocarpa from 21 populations spanning the entire distribution range.Distribution patterns based on nSSR data revealed that there are three genetic groups in P.lasiocarpa.This is consistent with the three phylogeographic breaks(Sichuan Basin,the Kaiyong Line and the 105°E line),where the Sichuan basin acts as the main barrier to gene flow between western and eastern groups.However,the distribution pattern based on ptDNA haplotypes poorly matched the phylogeographic breaks,and wind-dispersed seeds may be one of the main contributing factors.Species distribution modelling suggested a larger potential distribution in the last glacial maximum with a severe bottleneck during the last interglacial.A DIYABC model also suggested a population contraction and expansion for both western and eastern lineages.These results indicate that biological traits are likely to affect the evolutionary history of plants,and that nuclear molecular markers,which experience higher levels of gene flow,might be better indicators of phylogeographic breaks.
基金Under the auspices of National Natural Science Foundation of China(40771035)the Knowledge Innovation Project,Chinese Academy of Sciences(KZCX2-YW-Q06-03)
文摘In order to investigate the dew amounts and analysis formation conditions of dew by examining the correlations between dew amounts and meteorological factors in Carex lasiocarpa marsh in the Sanjiang Plain,the dew amounts and several meteorological factors were monitored during the growing season from June to September in 2005 and 2008 at the Sanjiang Mire Wetland Experimental Station,Chinese Academy of Sciences.Dew was collected by woodstick made by poplar tree in Carex lasiocarpa marsh and the amounts were estimated by subtraction method.The results indicated that average daily accumulated dew amounts reached the peak in August.The highest dew amounts in Carex lasiocarpa marsh were 0.201 mm/d,and the most frequent dew volume ranged from 0.08 mm/d to 0.14 mm/d.The amounts correlated positively with relative humidity,dew point temperature,and water vapor pressure.Whereas it correlated negatively with wind speed.The dew production in Carex lasiocarpa marsh more frequently occurred under the conditions of wind speed around 2.0 m/s,the water vapor pressure above 9 hPa;the relative humidity between 90% and 95% and dew point temperature above 6 ℃.
文摘利用超高效液相色谱四极杆飞行时间串联质谱联用(UPLC-Q-TOF-MS)代谢组学技术,对地涌金莲[Musella lasiocarpa(Franch.) C. Y. Wu ex H. W. Li]等6种芭蕉亚科(subfam. Musoideae)植物种子代谢物进行非靶向分析,并进一步对地涌金莲不同成熟期种子及不同组织的代谢物进行靶向分析,还对7种化合物进行了体外抗真菌活性检测。结果显示:地涌金莲种子代谢物与芭蕉亚科另5种植物差异明显。地涌金莲与蕉麻(Musa textilis Née)之间的显著差异代谢物最少(279),与朝天蕉(Musa velutina H. Wendl. et Drude)之间的显著差异代谢物最多(429);地涌金莲与另5种植物的共有显著差异代谢物数量为63,识别鉴定出37个代谢物,其中,4个二芳基庚烷类化合物在地涌金莲种子中的相对含量显著高于另5种植物。靶向分析结果显示:在地涌金莲4个成熟期种子中共鉴定到12个二芳基庚烷类显著差异代谢物,且多数化合物在黑色或深褐色种子中的相对含量最高。对地涌金莲不同组织中的二芳基庚烷类化合物含量进行比较,结果显示这类化合物多集中在地下组织(假茎、假茎皮、根)。抗真菌活性实验结果显示:2-methoxy-9-phenyl-1H-phenalen-1-one对尖孢镰刀菌(Fusarium oxysporum)、胶孢炭疽菌(Colletotrichum gloeosporioides)和链格孢菌(Alternaria alternata)的抑制率显著(P<0.05)高于其他化合物。综合分析结果显示:地涌金莲富含二芳基庚烷类化合物,且多数二芳基庚烷类化合物为苯基非那烯酮类化合物,且具有较高的抗真菌活性。
基金This study was supported by the National Basic Research Program of China(973 Program,2007CB-411600)National Natural Science Foundation of China(No.30670160).
