摘要
以地涌金莲(Musella lasiocarpa)吸芽为外植体建立了有效的快繁体系。外植体经灭菌处理后在MS+6-BA4.0 mg L-1+NAA 0.2 mg L-1+维生素C 150 mg L-1+10%椰子乳+3%蔗糖培养基上能进行不定芽的诱导和增殖,培养60 d后每个芽平均能产生4.10个不定芽。第6代增殖后,丛生芽的增殖系数可达4.23。生根培养基以1/2MS+NAA1.0 mg L-1+AC 50 mg L-1的效果较好。以沙:泥炭土:珍珠岩=1:1:1为基质移栽试管苗,成活率达到93.5%以上。经过12个月的组织培养已生产10 000多株试管苗。
An efficient and rapid micropropagation system by using auxiliary buds as explants has been established for Musella lasiocarpa. Murashige and Skoog （MS） medium supplemented with 4.0 mg L^-1 6-benzylaminopurine, 0.2 mg L^-1 1-napthaleneacetic acid, 150 mg L^-1 vitamin C, 10% coconut milk and 3% sucrose was suitable for shoot induction and proliferation from divided buds. Each explant produced an average of 4.10 shoots after 60-day culture. After the sixth subculture, the shoot proliferation rate of plantlets was 4.23-fold. The half-strength MS medium supplemented with 1.0 mg L^-1 indole-3-butyric acid and 150 mg L^-1 activated charcoal was suitable for rooting. When micropropagated plantlets with well-developed root systems were transferred to planting bed containing a mixture of sand, sieved peat and perlite （1：1：1; v/v） in a greenhouse, 93.5% of the plantlets survived. About 10 000 plantlets were produced successfully for field transfer after 12 months of culture initiation. This production system is useful for ex situ conservation and large-scale multiplication of M. lasiocarpa.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2007年第1期55-62,共8页
Journal of Tropical and Subtropical Botany
基金
Guangdong Incubation Foundation Grant(No.97FF05)
Guanzhou Key Technology Research and Development Program Grant(No.2003F1-E0021)
关键词
地涌金莲
组织培养
微繁殖
Musella lasiocarpa
Tissue culture
Micropropagation
