[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric ...[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric acid, thus providing resistant materials for breeding Fusarium wilt-resistant red edible seed watermel- on. [ Method] Using Zhongxin No. 1 red edible seed watermelon advemitious buds as screening materials and fusaric acid （FA） as a stress agent, in vitro screen- ing of Fusarium wilt-resistant red edible seed watermelon clonal variants and identification of Fusarium wilt-resistance of the germplasm resources of red edible seed watermelon were performed. [ Result] The results showed that the appropriate FA for in vitro screening of Fusarium wilt-resistant red edible seed watermelon vari- ants was 15 mg/L. In vitro screening system for Fusarium wilt-resistant red edible seed watermelon variants was established preliminarily and FA-resistant regenera- ted plants were obtained. Among the 36 germplasm resources of red edible seed watermelon, there were 2 highly resistant materials, 6 moderately resistant materi- als, 11 slightly resistant materials and 17 highly susceptible materials. [ Conclusion] This study confirmed preliminarily that in vitro screening method is effective for obtaining resistant materials of red edible seed watermelon.展开更多
Objective:To determine the chemical profile and steroids composition of the medicinally important plant Aerva lanata(A.lanata) L.Methods:Preliminary phytochemicul screening was done by the method as Harborne described...Objective:To determine the chemical profile and steroids composition of the medicinally important plant Aerva lanata(A.lanata) L.Methods:Preliminary phytochemicul screening was done by the method as Harborne described.HPTLC studies were canied out as Harborne and Wagner et al described.The Ethyl acetate-ethanol-water(8:2:1.2) was employed as mobile phase for glycosides.Results:The desired aim was achieved using Chloroform-acetone(8:2) as the mobile phase.The methanolic extract of stem,leaves,root,flower and seeds of A.lanata showed the presence of 30 different types of steroids with 30 different Rf values from 0.04 to 0.97. Maximum number(11) of steroids has been observed in leaves followed by root(10).Conclusions: HPTLC profile of steroids has been chosen here to reveal the diversity existing in A.lanata.Such finger printing is useful in differentiating the species from the adulterant and act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies.展开更多
Objective:To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva Lanata（A.lanata） stem.Methods:During the preliminary phytochemical analysis,the aqueous extract of A...Objective:To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva Lanata（A.lanata） stem.Methods:During the preliminary phytochemical analysis,the aqueous extract of A.Ianata was screened for the presence of carbohydrates,proteins,phenolic compounds,oil and fats,saponins,flavonoids,alkaloids. tannins and phytosterols.Antioxidant activity of the extract was determined by 2.2-dipbenyl- 1-picrylhydrazyl radical scavenging activity,metal chelating activity,reducing power activity and DNA damage inhibition activity.Analysis of phenolic compounds was performed by FolinCiocaiteau reagent method and gradient high performance liquid chromatography technique. Results:Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins.flavonoids.tannins and phytosterols as major phytochemical groups.The extract exhibited high 2.2—diphenyl-1-picrylhydrazyl radical scavenging activity(IC<sub>50</sub>= 110.74μg/ mL).metal chelating activity(IC<sub>50</sub>= 758.17μg/mL).reducing power activity and DIA damage inhibition efficiency.The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid（3,4,3-OH）,apigenin-7—O-glucoside tapigetrin), quercetin-3—O-rutinoside（rutin） and myricetin（3,5,7,3,4,5-OH）by high performance liquid chromatography analysis.The extract was found non toxic towards human erythrocytes in the hemolytic assay(IC<sub>50</sub>= 24.89 mg/mL).Conclusions:These results conclud that A.lanata stem possesses high antioxidant activity and can he used for the development of natural and sale antioxidant compounds.展开更多
A rapid assessment on the status and distribution patterns of coarse woody debris （CWD） was conducted by laying five curvilinear transects （transects A, B, C, D and E; major trails）, along an altitudinal gradient ...A rapid assessment on the status and distribution patterns of coarse woody debris （CWD） was conducted by laying five curvilinear transects （transects A, B, C, D and E; major trails）, along an altitudinal gradient starting from 900 to 2 600 m, in three major watersheds （Charigad, Dogarhigad and upper Gosigad） of Goriganga catchment in Askot Wildlife Sanctuary, India. At every 100 m rise in altitude a hectare plot （100 m x 100 m） was selected. Results showed that the percentage contribution by different succession phases was in the decreasing order： phase I 〉 phase II 〉 phase IV 〉 phase III for snags, and phase III〉 phase II 〉 phase IV 〉 phase I for logs. Snag density in chir pine forest was high in transect A （11 snags＇ha^-1） at 1 500 m, and the value in rianj oak forest was high in transect B （10 snags.ha-l） at 2 300 m. The total available mass of snags and logs in chit pine forest was 13.9 t, of which snags mass accounted for 41% of the total mass and logs mass for 59%. While the total value was 5.6 t in rianj oak forest, of which snags and logs accounted for 60% and 40% of the total mass, respectively. Moreover, the presence of CWD in the study area if not harvested, would provide a great opportunity to the orchids in future to flourish by providing protection. The high densities of snags and logs in chir pine forest at mid-altitudinal zone led to less species richness and lower density of ground flora as the zone receives more light, accompanying with lower soil moisture, and thus only the dominant species occupy the habitat.展开更多
Objective:Aerva lanata (Linn) Juss ex Schult,family Amaranthaceae,is a common wayside weed.The herb is accepted by the Ayurvedic Formulary of India.This study was undertaken to establish morphoanatomic and physiochemi...Objective:Aerva lanata (Linn) Juss ex Schult,family Amaranthaceae,is a common wayside weed.The herb is accepted by the Ayurvedic Formulary of India.This study was undertaken to establish morphoanatomic and physiochemical standards of A.lanata.Methods:Leaf constants and high-performance thin layer chromatography fingerprint profiles of A.lanata were performed.Results and conclusion:The physico-chemical,morphologic,and histologic parameters presented in this paper may be proposed as standards to establish the authenticity of A.lanata and help differentiate it from other species such as Aerva tomentosa.展开更多
A rapid assessment on the status and distribution patterns of coarse woody debris (CWD) was conducted by laying five curvilinear transects (transects A, B, C, D and E; major trails), along an altitudinal gradient star...A rapid assessment on the status and distribution patterns of coarse woody debris (CWD) was conducted by laying five curvilinear transects (transects A, B, C, D and E; major trails), along an altitudinal gradient starting from 900 to 2 600 m, in three major watersheds (Charigad, Dogarhigad and upper Gosigad) of Goriganga catchment in Askot Wildlife Sanctuary, India. At every 100 m rise in altitude a hectare plot (100 m x 100 m) was selected. Results showed that the percentage contribution by different succession phases was in the decreasing order: phase I > phase II > phase IV > phase III for snags, and phase III > phase II > phase IV > phase I for logs. Snag density in chir pine forest was high in transect A (11 snags·ha-1) at 1 500 m, and the value in rianj oak forest was high in transect B (10 snags·ha-1) at 2 300 m. The total available mass of snags and logs in chir pine forest was 13.9 t, of which snags mass accounted for 41% of the total mass and logs mass for 59%. While the total value was 5.6 t in rianj oak forest, of which snags and logs accounted for 60% and 40% of the total mass, respectively. Moreover, the presence of CWD in the study area if not harvested, would provide a great opportunity to the orchids in future to flourish by providing protection. The high densities of snags and logs in chir pine forest at mid-altitudinal zone led to less species richness and lower density of ground flora as the zone receives more light, accompanying with lower soil moisture, and thus only the dominant species occupy the habitat.展开更多
We previously developed an HPLC method for determination of lanatoside C, digoxin and α-acetyldigoxin in digitalis glycosides isolated from Digitalis lanata leaves. Here, we present an improved HPLC-UV method to dete...We previously developed an HPLC method for determination of lanatoside C, digoxin and α-acetyldigoxin in digitalis glycosides isolated from Digitalis lanata leaves. Here, we present an improved HPLC-UV method to determine those compounds and deslanoside. We used the improved method to examine the effects of various pretreatments on the amounts of the four compounds isolated from the leaves, with the aim of maximizing the yield of digoxin. Leaves were extracted with 50% methanol, followed by clean-up on a Sep-Pak C18 cartridge prior to HPLC analysis. The amounts of lanatoside C, digoxin and α-acetyldigoxin per 100 mg of the leaves without pretreatment were 115.6, 7.45 and 23.8 μg, respectively (deslanoside was not detected). Pretreatment with acetic acid, which activated deglucosylation mediated by digilanidase present in the leaves, increased the amounts of digoxin and α-acetyldigoxin, while lanatoside C and deslanoside were not detected. Pretreatment with sodium methoxide, which hydrolyzed lanatoside C to deslanoside, increased the yields of deslanoside and digoxin, while lanatoside C and α-acetyldigoxin were not detected. The combination of both pretreatments afforded only digoxin in a yield of 115.1 μg/100 mg leaves. Use of the combined pretreatments appears to be effective for maximizing the yield of digoxin from the leaves.展开更多
Objective Nephrolithiasis is a common urological disease. This study aims to evaluate the preventive and therapeutic effects of hydro-alcoholic extract of Aerva lanata(L.) roots(HAEAL) on ethylene glycol-induced nephr...Objective Nephrolithiasis is a common urological disease. This study aims to evaluate the preventive and therapeutic effects of hydro-alcoholic extract of Aerva lanata(L.) roots(HAEAL) on ethylene glycol-induced nephrolithiasis in rats.Methods Fifty grams of shade-dried coarsely powdered Aerva lanata(L.) root was successively extracted with organic solvents in increasing order of polarity [petroleum ether(60-80 ℃), chloroform, and ethanol] using a Soxhlet apparatus, and then concentrated. Physical tests including nature, color, odor, and texture were performed on the herbal suspension. In vitro nephrolithiasis assessment was performed by nucleation assay, aggregation assay, and crystal growth assay. Thirty adult male Wistar albino rats were randomly divided into five groups(six rats in each group). Group 1: negative control group without induction or treatment till day 28. Group 2: positive control group receiving a daily oral solution of 0.75% ethylene glycol till day 14, and mixed with distilled water till day 28. Group 3: standard group receiving a daily oral solution of 0.75% ethylene glycol till day 14 and Cystone(750 mg/kg) from day 15 to day 28. Group 4: low dose HAEAL group receiving a daily oral solution of 0.75%ethylene glycol till day 14, and 400 mg/kg HAEAL from day 15 to day 28(1 mL per day). Group 5: high dose HAEAL group receiving a daily oral solution of 0.75% ethylene glycol till day 14,and 800 mg/kg HAEAL from day 15 to day 28(1 mL per day). Urine(urine volume, pH value,appearance, odor, and turbidity) examination and serum test were performed. On day 29, the kidneys were dissected, and histopathology examination was performed to determine the degree of tubular injury.Results The suspension showed stability and aroma with no turbidity at room temperature.The suspension did not show changes in color and odor until day 3, indicating that the preparation was stable for 72 h. Body weight decreased in the positive control group indicating stone formation and changes in water intake. Both standard and HAEAL treatments restored the body weight to normal levels after treatment, indicating the beneficial effects of the treatment. Histopathological examination revealed no significant findings in the negative control group, whereas the positive control group showed inflammation in the kidney parenchyma.Compared with positive control group, there was increase in urine volume and excretion of urinary constituents such as calcium and oxalate(P < 0.01) as well as improved clearance rate(P < 0.05) in HAEAL treatment groups, in addition, the urine pH value of HAEAL groups was increased.Conclusion HAEAL reduced nephrolithiasis formation and had a diuretic effect, which could be used to promote the expulsion of stones. Further studies are needed to enhance the stability of the suspension for the production of better pharmaceutical formulations.展开更多
Objective: Digoxin is a therapeutic cardenolide widely used to treat various heart conditions such as atrial flutter, atrial fibrillation and heart failure in both Western as well as Chinese medicine. Digoxin is extra...Objective: Digoxin is a therapeutic cardenolide widely used to treat various heart conditions such as atrial flutter, atrial fibrillation and heart failure in both Western as well as Chinese medicine. Digoxin is extracted from cultivated Digitalis lanata Ehrh. plants, known as Mao Hua Yang Di Huang in Chinese medicine. This manuscript presents two studies that were conducted to optimize the cultivation conditions for digoxin production in the TCM Mao Hua Yan Di Huang in a greenhouse under GAP conditions.Methods: Two experiments were designed in which 4 growth conditions were compared. Levels of digoxin, gitoxin, digitoxin, α-acetyldigoxin, β-acetyl-digoxin were measured using HPLC-UV and compared between the conditions.Results: Normal soil, no CO_2 enrichment combined with a cold shock was found to be the optimal condition for producing digoxin in the first experiment. Gitoxin content was significantly lower in plants grown in this condition. Mechanical stress as well as the time of harvesting showed no statistically significant differences in the production of cardenolides. In the second experiment the optimal condition was found to be a combination of cold nights, sun screen, fertilizer use and no milled soil.Conclusion: This study shows that digoxin production can be increased by controlling the growth conditions of D. lanata Ehrh. The effect of cold was important in both experiments for improving digoxin production. Cultivating Chinese herbal medicines in optimized greenhouse conditions might be an economically attractive alternative to regular open air cultivation.展开更多
基金Supported by National Space Breeding Project of China(2006HT100113)China Agriculture Research System(CARS-26)Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(GNK2013YZ26)
文摘[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric acid, thus providing resistant materials for breeding Fusarium wilt-resistant red edible seed watermel- on. [ Method] Using Zhongxin No. 1 red edible seed watermelon advemitious buds as screening materials and fusaric acid （FA） as a stress agent, in vitro screen- ing of Fusarium wilt-resistant red edible seed watermelon clonal variants and identification of Fusarium wilt-resistance of the germplasm resources of red edible seed watermelon were performed. [ Result] The results showed that the appropriate FA for in vitro screening of Fusarium wilt-resistant red edible seed watermelon vari- ants was 15 mg/L. In vitro screening system for Fusarium wilt-resistant red edible seed watermelon variants was established preliminarily and FA-resistant regenera- ted plants were obtained. Among the 36 germplasm resources of red edible seed watermelon, there were 2 highly resistant materials, 6 moderately resistant materi- als, 11 slightly resistant materials and 17 highly susceptible materials. [ Conclusion] This study confirmed preliminarily that in vitro screening method is effective for obtaining resistant materials of red edible seed watermelon.
文摘Objective:To determine the chemical profile and steroids composition of the medicinally important plant Aerva lanata(A.lanata) L.Methods:Preliminary phytochemicul screening was done by the method as Harborne described.HPTLC studies were canied out as Harborne and Wagner et al described.The Ethyl acetate-ethanol-water(8:2:1.2) was employed as mobile phase for glycosides.Results:The desired aim was achieved using Chloroform-acetone(8:2) as the mobile phase.The methanolic extract of stem,leaves,root,flower and seeds of A.lanata showed the presence of 30 different types of steroids with 30 different Rf values from 0.04 to 0.97. Maximum number(11) of steroids has been observed in leaves followed by root(10).Conclusions: HPTLC profile of steroids has been chosen here to reveal the diversity existing in A.lanata.Such finger printing is useful in differentiating the species from the adulterant and act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies.
文摘Objective:To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva Lanata（A.lanata） stem.Methods:During the preliminary phytochemical analysis,the aqueous extract of A.Ianata was screened for the presence of carbohydrates,proteins,phenolic compounds,oil and fats,saponins,flavonoids,alkaloids. tannins and phytosterols.Antioxidant activity of the extract was determined by 2.2-dipbenyl- 1-picrylhydrazyl radical scavenging activity,metal chelating activity,reducing power activity and DNA damage inhibition activity.Analysis of phenolic compounds was performed by FolinCiocaiteau reagent method and gradient high performance liquid chromatography technique. Results:Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins.flavonoids.tannins and phytosterols as major phytochemical groups.The extract exhibited high 2.2—diphenyl-1-picrylhydrazyl radical scavenging activity(IC<sub>50</sub>= 110.74μg/ mL).metal chelating activity(IC<sub>50</sub>= 758.17μg/mL).reducing power activity and DIA damage inhibition efficiency.The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid（3,4,3-OH）,apigenin-7—O-glucoside tapigetrin), quercetin-3—O-rutinoside（rutin） and myricetin（3,5,7,3,4,5-OH）by high performance liquid chromatography analysis.The extract was found non toxic towards human erythrocytes in the hemolytic assay(IC<sub>50</sub>= 24.89 mg/mL).Conclusions:These results conclud that A.lanata stem possesses high antioxidant activity and can he used for the development of natural and sale antioxidant compounds.
文摘A rapid assessment on the status and distribution patterns of coarse woody debris （CWD） was conducted by laying five curvilinear transects （transects A, B, C, D and E; major trails）, along an altitudinal gradient starting from 900 to 2 600 m, in three major watersheds （Charigad, Dogarhigad and upper Gosigad） of Goriganga catchment in Askot Wildlife Sanctuary, India. At every 100 m rise in altitude a hectare plot （100 m x 100 m） was selected. Results showed that the percentage contribution by different succession phases was in the decreasing order： phase I 〉 phase II 〉 phase IV 〉 phase III for snags, and phase III〉 phase II 〉 phase IV 〉 phase I for logs. Snag density in chir pine forest was high in transect A （11 snags＇ha^-1） at 1 500 m, and the value in rianj oak forest was high in transect B （10 snags.ha-l） at 2 300 m. The total available mass of snags and logs in chit pine forest was 13.9 t, of which snags mass accounted for 41% of the total mass and logs mass for 59%. While the total value was 5.6 t in rianj oak forest, of which snags and logs accounted for 60% and 40% of the total mass, respectively. Moreover, the presence of CWD in the study area if not harvested, would provide a great opportunity to the orchids in future to flourish by providing protection. The high densities of snags and logs in chir pine forest at mid-altitudinal zone led to less species richness and lower density of ground flora as the zone receives more light, accompanying with lower soil moisture, and thus only the dominant species occupy the habitat.
基金The authors thank the Principal of Shivalik College of Pharmacy,Nangal and BITS,Ranchi for providing the facilities for this research work.Support was also provided by Capital Application Project on Clinical Characteristics of Science and Technology Commission of Beijing Municipality(No.Z111107058811056)by the Planned Project on Beijing Traditional Chinese Medicine“Inheritance of 3 t 3 Program”of the Beijing Chinese Medicine Administration Bureau.
文摘Objective:Aerva lanata (Linn) Juss ex Schult,family Amaranthaceae,is a common wayside weed.The herb is accepted by the Ayurvedic Formulary of India.This study was undertaken to establish morphoanatomic and physiochemical standards of A.lanata.Methods:Leaf constants and high-performance thin layer chromatography fingerprint profiles of A.lanata were performed.Results and conclusion:The physico-chemical,morphologic,and histologic parameters presented in this paper may be proposed as standards to establish the authenticity of A.lanata and help differentiate it from other species such as Aerva tomentosa.
文摘A rapid assessment on the status and distribution patterns of coarse woody debris (CWD) was conducted by laying five curvilinear transects (transects A, B, C, D and E; major trails), along an altitudinal gradient starting from 900 to 2 600 m, in three major watersheds (Charigad, Dogarhigad and upper Gosigad) of Goriganga catchment in Askot Wildlife Sanctuary, India. At every 100 m rise in altitude a hectare plot (100 m x 100 m) was selected. Results showed that the percentage contribution by different succession phases was in the decreasing order: phase I > phase II > phase IV > phase III for snags, and phase III > phase II > phase IV > phase I for logs. Snag density in chir pine forest was high in transect A (11 snags·ha-1) at 1 500 m, and the value in rianj oak forest was high in transect B (10 snags·ha-1) at 2 300 m. The total available mass of snags and logs in chir pine forest was 13.9 t, of which snags mass accounted for 41% of the total mass and logs mass for 59%. While the total value was 5.6 t in rianj oak forest, of which snags and logs accounted for 60% and 40% of the total mass, respectively. Moreover, the presence of CWD in the study area if not harvested, would provide a great opportunity to the orchids in future to flourish by providing protection. The high densities of snags and logs in chir pine forest at mid-altitudinal zone led to less species richness and lower density of ground flora as the zone receives more light, accompanying with lower soil moisture, and thus only the dominant species occupy the habitat.
文摘We previously developed an HPLC method for determination of lanatoside C, digoxin and α-acetyldigoxin in digitalis glycosides isolated from Digitalis lanata leaves. Here, we present an improved HPLC-UV method to determine those compounds and deslanoside. We used the improved method to examine the effects of various pretreatments on the amounts of the four compounds isolated from the leaves, with the aim of maximizing the yield of digoxin. Leaves were extracted with 50% methanol, followed by clean-up on a Sep-Pak C18 cartridge prior to HPLC analysis. The amounts of lanatoside C, digoxin and α-acetyldigoxin per 100 mg of the leaves without pretreatment were 115.6, 7.45 and 23.8 μg, respectively (deslanoside was not detected). Pretreatment with acetic acid, which activated deglucosylation mediated by digilanidase present in the leaves, increased the amounts of digoxin and α-acetyldigoxin, while lanatoside C and deslanoside were not detected. Pretreatment with sodium methoxide, which hydrolyzed lanatoside C to deslanoside, increased the yields of deslanoside and digoxin, while lanatoside C and α-acetyldigoxin were not detected. The combination of both pretreatments afforded only digoxin in a yield of 115.1 μg/100 mg leaves. Use of the combined pretreatments appears to be effective for maximizing the yield of digoxin from the leaves.
文摘Objective Nephrolithiasis is a common urological disease. This study aims to evaluate the preventive and therapeutic effects of hydro-alcoholic extract of Aerva lanata(L.) roots(HAEAL) on ethylene glycol-induced nephrolithiasis in rats.Methods Fifty grams of shade-dried coarsely powdered Aerva lanata(L.) root was successively extracted with organic solvents in increasing order of polarity [petroleum ether(60-80 ℃), chloroform, and ethanol] using a Soxhlet apparatus, and then concentrated. Physical tests including nature, color, odor, and texture were performed on the herbal suspension. In vitro nephrolithiasis assessment was performed by nucleation assay, aggregation assay, and crystal growth assay. Thirty adult male Wistar albino rats were randomly divided into five groups(six rats in each group). Group 1: negative control group without induction or treatment till day 28. Group 2: positive control group receiving a daily oral solution of 0.75% ethylene glycol till day 14, and mixed with distilled water till day 28. Group 3: standard group receiving a daily oral solution of 0.75% ethylene glycol till day 14 and Cystone(750 mg/kg) from day 15 to day 28. Group 4: low dose HAEAL group receiving a daily oral solution of 0.75%ethylene glycol till day 14, and 400 mg/kg HAEAL from day 15 to day 28(1 mL per day). Group 5: high dose HAEAL group receiving a daily oral solution of 0.75% ethylene glycol till day 14,and 800 mg/kg HAEAL from day 15 to day 28(1 mL per day). Urine(urine volume, pH value,appearance, odor, and turbidity) examination and serum test were performed. On day 29, the kidneys were dissected, and histopathology examination was performed to determine the degree of tubular injury.Results The suspension showed stability and aroma with no turbidity at room temperature.The suspension did not show changes in color and odor until day 3, indicating that the preparation was stable for 72 h. Body weight decreased in the positive control group indicating stone formation and changes in water intake. Both standard and HAEAL treatments restored the body weight to normal levels after treatment, indicating the beneficial effects of the treatment. Histopathological examination revealed no significant findings in the negative control group, whereas the positive control group showed inflammation in the kidney parenchyma.Compared with positive control group, there was increase in urine volume and excretion of urinary constituents such as calcium and oxalate(P < 0.01) as well as improved clearance rate(P < 0.05) in HAEAL treatment groups, in addition, the urine pH value of HAEAL groups was increased.Conclusion HAEAL reduced nephrolithiasis formation and had a diuretic effect, which could be used to promote the expulsion of stones. Further studies are needed to enhance the stability of the suspension for the production of better pharmaceutical formulations.
基金the city of Emmen, the province Drenthe (KEI program)"Kenniscentrum plantenstoffen"+1 种基金"LTO Noord Projecten" in the Netherlandspartly funded by Boehringer Ingelheim Pharma Gmb H & Co.KG
文摘Objective: Digoxin is a therapeutic cardenolide widely used to treat various heart conditions such as atrial flutter, atrial fibrillation and heart failure in both Western as well as Chinese medicine. Digoxin is extracted from cultivated Digitalis lanata Ehrh. plants, known as Mao Hua Yang Di Huang in Chinese medicine. This manuscript presents two studies that were conducted to optimize the cultivation conditions for digoxin production in the TCM Mao Hua Yan Di Huang in a greenhouse under GAP conditions.Methods: Two experiments were designed in which 4 growth conditions were compared. Levels of digoxin, gitoxin, digitoxin, α-acetyldigoxin, β-acetyl-digoxin were measured using HPLC-UV and compared between the conditions.Results: Normal soil, no CO_2 enrichment combined with a cold shock was found to be the optimal condition for producing digoxin in the first experiment. Gitoxin content was significantly lower in plants grown in this condition. Mechanical stress as well as the time of harvesting showed no statistically significant differences in the production of cardenolides. In the second experiment the optimal condition was found to be a combination of cold nights, sun screen, fertilizer use and no milled soil.Conclusion: This study shows that digoxin production can be increased by controlling the growth conditions of D. lanata Ehrh. The effect of cold was important in both experiments for improving digoxin production. Cultivating Chinese herbal medicines in optimized greenhouse conditions might be an economically attractive alternative to regular open air cultivation.
