摘要
Objective:To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva Lanata（A.lanata） stem.Methods:During the preliminary phytochemical analysis,the aqueous extract of A.Ianata was screened for the presence of carbohydrates,proteins,phenolic compounds,oil and fats,saponins,flavonoids,alkaloids. tannins and phytosterols.Antioxidant activity of the extract was determined by 2.2-dipbenyl- 1-picrylhydrazyl radical scavenging activity,metal chelating activity,reducing power activity and DNA damage inhibition activity.Analysis of phenolic compounds was performed by FolinCiocaiteau reagent method and gradient high performance liquid chromatography technique. Results:Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins.flavonoids.tannins and phytosterols as major phytochemical groups.The extract exhibited high 2.2—diphenyl-1-picrylhydrazyl radical scavenging activity(IC<sub>50</sub>= 110.74μg/ mL).metal chelating activity(IC<sub>50</sub>= 758.17μg/mL).reducing power activity and DIA damage inhibition efficiency.The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid（3,4,3-OH）,apigenin-7—O-glucoside tapigetrin), quercetin-3—O-rutinoside（rutin） and myricetin（3,5,7,3,4,5-OH）by high performance liquid chromatography analysis.The extract was found non toxic towards human erythrocytes in the hemolytic assay(IC<sub>50</sub>= 24.89 mg/mL).Conclusions:These results conclud that A.lanata stem possesses high antioxidant activity and can he used for the development of natural and sale antioxidant compounds.
Objective:To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva Lanata（A.lanata） stem.Methods:During the preliminary phytochemical analysis,the aqueous extract of A.Ianata was screened for the presence of carbohydrates,proteins,phenolic compounds,oil and fats,saponins,flavonoids,alkaloids. tannins and phytosterols.Antioxidant activity of the extract was determined by 2.2-dipbenyl- 1-picrylhydrazyl radical scavenging activity,metal chelating activity,reducing power activity and DNA damage inhibition activity.Analysis of phenolic compounds was performed by FolinCiocaiteau reagent method and gradient high performance liquid chromatography technique. Results:Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins.flavonoids.tannins and phytosterols as major phytochemical groups.The extract exhibited high 2.2—diphenyl-1-picrylhydrazyl radical scavenging activity(IC50= 110.74μg/ mL).metal chelating activity(IC50= 758.17μg/mL).reducing power activity and DIA damage inhibition efficiency.The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid（3,4,3-OH）,apigenin-7—O-glucoside tapigetrin), quercetin-3—O-rutinoside（rutin） and myricetin（3,5,7,3,4,5-OH）by high performance liquid chromatography analysis.The extract was found non toxic towards human erythrocytes in the hemolytic assay(IC50= 24.89 mg/mL).Conclusions:These results conclud that A.lanata stem possesses high antioxidant activity and can he used for the development of natural and sale antioxidant compounds.
