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Limit analysis method for active earth pressure on laggings between stabilizing piles 被引量:2
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作者 WANG Ming-min WU Shu-guang WANG Gui-lin 《Journal of Mountain Science》 SCIE CSCD 2017年第1期196-204,共9页
Stabilizing pile is a kind of earth shoring structure frequently used in slope engineering. When the piles have cantilever segments above the ground,laggings are usually installed to avoid collapse of soil between pil... Stabilizing pile is a kind of earth shoring structure frequently used in slope engineering. When the piles have cantilever segments above the ground,laggings are usually installed to avoid collapse of soil between piles. Evaluating the earth pressure acting on laggings is of great importance in design process.Since laggings are usually less stiff than piles,the lateral pressure on lagging is much closer to active earth pressure. In order to estimate the lateral earth pressure on lagging more accurately,first,a model test of cantilever stabilizing pile and lagging systems was carried out. Then,basing the experimental results a three-dimensional sliding wedge model was established. Last,the calculation process of the total active force on lagging is presented based on the kinematic approach of limit analysis. A comparison is made between the total active force on lagging calculated by the formula presented in this study and the force on a same-size rigid retaining wall obtained from Rankine's theory. It is found that the proposed method fits well with the experimental results.Parametric studies show that the total active force on lagging increases with the growth of the lagging height and the lagging clear span; while decreases asthe soil internal friction angle and soil cohesion increase. 展开更多
关键词 Stabilizing pile lagging Active earth pressure Limit analysis method Sliding surface
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Lagging and Its Kinetic Mechanism of Hydrocarbon Re-generation from Organic Matters in Coal
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作者 Qin Yong Zhang Yousheng Zhu Yanming Fan Bingheng Jiang Bo Li Tianzhong College of Mineral Resources and Environment Science, China University of Mining and Technology, Xuzhou 221008 《Journal of Earth Science》 SCIE CAS CSCD 2000年第1期86-90,共5页
Based on the composite analysis of the coal sample series with natural and artificial maturation, the lagging and its kinetic mechanism of the hydrocarbon re-generation from the organic matters in coals were studied u... Based on the composite analysis of the coal sample series with natural and artificial maturation, the lagging and its kinetic mechanism of the hydrocarbon re-generation from the organic matters in coals were studied using the Rock-Eval gas chromatogram (PyGC) method. The results show that the maturation at the hydrocarbon regeneration peak shifts regularly forward with increasing the starting maturation and the deadline of the hydrocarbon regeneration lies about at 4.0% R o. The difference value between the peak and starting maturation of the hydrocarbon re-generation develops in a parabolalike pattern with increasing the starting maturation, and the resolute and relative laggings evolve in stage, from which the lagging depth could be predicted. The peak halfwidth of the hydrocarbon re-generation curve develops as the starting maturation increases, which might indicate that the hydrocarbonderived rocks with the starting maturation lied about at oilgenerated peak might be relatively high in the hydrocarbonregenerated amount. In the meantime, the mean reactivated energy of the coal samples with starting maturation develops in four stages that are highly consistent with those of the hydrocarbonregenerated amount and lagging, which indicated that the hydrocarbon re-generation is strictly controlled by the geochemical mechanism of the reactive kinetics. 展开更多
关键词 COAL organic matter hydrocarbon re-generation lagging kinetics.
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The Lagging behind Security Ties and the Way out
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作者 Sun Cheng is a Researcher Professor at China Institute of International Studies. 《Contemporary International Relations》 2003年第11期10-16,共7页
The present Sino-Japanese relations have developed smoothly and achieved marked results, but some problems are yet to be, solved. One of them is that, compared with the closer economic ties, political and security rel... The present Sino-Japanese relations have developed smoothly and achieved marked results, but some problems are yet to be, solved. One of them is that, compared with the closer economic ties, political and security relations between the two states are lagging behind, in which security relations in particular have obstructed the deepening of ties between the two nations. As two major powers in East Asia, the ties be- 展开更多
关键词 that HAVE into The lagging behind Security Ties and the Way out SECURITY
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Abrasive WaterJet Machining of Thick Carrara Marble: Cutting Performance vs. Profile, Lagging and WaterJet Angle Assessments
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作者 Mohammad S. Alsoufi Dhia K. Suker +1 位作者 Mohammed W. Alhazmi Sufyan Azam 《Materials Sciences and Applications》 2017年第5期361-375,共15页
This paper deals with an assessment of the machined surface created by abrasive waterjet technology regarding its cutting performance versus profile, lagging and waterjet angle assessments. The results of the experime... This paper deals with an assessment of the machined surface created by abrasive waterjet technology regarding its cutting performance versus profile, lagging and waterjet angle assessments. The results of the experiments presented in this study are with regard to Carrara marble. The machined surfaces were measured in seven different locations across a 40 mm depth of cut by a high precision contact-type profilometer and thus assessed using the standardized amplitude parameters of the profile distribution. The lagging and waterjet angle were also evaluated by creating a digital photo of the machined surface together with a reference gauge. The existence of machining marks on the machined surface has been mostly noticeable in the bottom zone around ~20 mm depth of cut down to jet exit. This investigation leads to a conclusion that, stand-off distance and traverse rate play the roles of the utmost importance in considerations of the machined surface quality in contrast to abrasive mass flow rate. In addition, while the striation zone (rough surface) cannot be eliminated entirely, by selecting proper process parameters, a smooth cutting machined surface can be accomplished. 展开更多
关键词 SKEWNESS KURTOSIS lagging Waterjet ANGLE ABRASIVE WaterJet Carrara MARBLE
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Animation industry “still lagging”
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《China's Foreign Trade》 2012年第6期40-40,共1页
The animation and cartoonindustry is booming in China but experts say there is still a long way to go before it will make a big impression on the international market, according to a China Daily report.
关键词 ANIMATION STILL lagging ANIMATION DAILY IMPRESSION
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Advancing and lagging effects of weather conditions on intercity traffic volume: A geographically weighted regression analysis in the Guangdong-Hong Kong-Macao Greater Bay Area
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作者 Peiqun Lin Yuanbo Hong +1 位作者 Yitao He Mingyang Pei 《International Journal of Transportation Science and Technology》 2024年第1期58-76,共19页
With the rapid expansion of urban areas,intercity highways have become crucial for daily transportation.Traffic administrators and planners increasingly rely on evaluating high way traffic volume.This paper aims to in... With the rapid expansion of urban areas,intercity highways have become crucial for daily transportation.Traffic administrators and planners increasingly rely on evaluating high way traffic volume.This paper aims to investigate the relationship between various factors and intercity traffic volume,with a specific focus on exploring the advancing and lagging effects of weather conditions on traffic volume in the districts of urban agglomerations.Using multiple data sources in the Guangdong-Hong Kong-Macao Greater Bay Area,includ ing weather factors(i.e.,rain,temperature,wind,and visibility),traffic factors(i.e.,total traffic volume and travel time),and other factors(i.e.,node degree,hub cities,and time of day),a mixed geographically weighted regression(MGWR)model is applied to examine the spatial heterogeneity of these factors.The results show that intercity traffic volume is influenced by weather,traffic,and other factors.Additionally,the advancing and lagging effects of different weather factors exhibit spatial heterogeneity across districts.Moreover,the weather lagging effect has a more significant impact than the advancing effect on intercity traffic volume.These findings provide valuable insights into the impact of weather on intercity travel volume and offer precise traffic guidance for intercity travelers. 展开更多
关键词 Intercity traffic volume Guangdong-Hong Kong-Macao Greater Bay Area Advancing and lagging effects of weather conditions Mixed geographically weighted regression Spatiotemporal heterogeneity
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EQ^(rot)_1 Nonconforming Finite Element Method for Nonlinear Dual Phase Lagging Heat Conduction Equations 被引量:6
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作者 Yan-min Zhao Fen-ling Wang Dong-yang Shi 《Acta Mathematicae Applicatae Sinica》 SCIE CSCD 2013年第1期201-214,共14页
EQrot nonconforming finite element approximation to a class of nonlinear dual phase lagging heat conduction equations is discussed for semi-discrete and fully-discrete schemes. By use of a special property, that is, t... EQrot nonconforming finite element approximation to a class of nonlinear dual phase lagging heat conduction equations is discussed for semi-discrete and fully-discrete schemes. By use of a special property, that is, the consistency error of this element is of order O(h2) one order higher than its interpolation error O(h), the superclose results of order O(h2) in broken Hi-norm are obtained. At the same time, the global superconvergence in broken Hi-norm is deduced by interpolation postprocessing technique. Moreover, the extrapolation result with order O(h4) is derived by constructing a new interpolation postprocessing operator and extrapolation scheme based on the known asymptotic expansion formulas of EQrot element. Finally, optimal error estimate is gained for a proposed fully-discrete scheme by different approaches from the previous literature. 展开更多
关键词 nonlinear dual phase lagging heat conduction equations EQrot nonconforming finite element superclose and superconvergence EXTRAPOLATION semi-discrete and fully-discrete schemes
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Numerical investigation on the responses of existing single piles due to adjacent twin tunneling considering the lagging distance 被引量:1
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作者 Chana Phutthananon Somkiat Lertkultanon +3 位作者 Pornkasem Jongpradist Ochok Duangsano Suched Likitlersuang Pitthaya Jamsawang 《Underground Space》 SCIE EI CSCD 2023年第4期171-188,共18页
This paper presents an assessment of the influence of the lagging distance between two horizontal tunnel faces of the side-by-side twin tunnels on the responses of the adjacent existing single pile by a series of thre... This paper presents an assessment of the influence of the lagging distance between two horizontal tunnel faces of the side-by-side twin tunnels on the responses of the adjacent existing single pile by a series of three-dimensional numerical analyses.Two different relative positions between the pile tip and the tunnel are considered to cover the short and long pile behaviors.The responses of the existing pile in terms of pile head settlement,axial force,lateral movement and bending moment are considered and discussed.The numerical results indicate that the lagging distance between twin tunnel faces significantly affects not only the soil movements but also the responses of the existing single pile.The critical case that produces unsatisfactory pile responses due to twin tunneling is when the lagging distance between the second tunnel and the preceding tunnel equals to the shield length.It is recommended that the lagging distance be not less than three times of shield length when the two tunnels need to be concurrently excavated. 展开更多
关键词 Twin tunnels Tunnel lagging distance Existing pile Finite element analysis Pile responses
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Spatio-temporal patterns and climatic drivers of spring phenology in eight forest communities across the north-south transitional zone of China 被引量:1
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作者 ZHU Wenbin LU Yu 《Journal of Geographical Sciences》 2025年第1期17-38,共22页
The Qinba Mountains are climatically and ecologically recognized as the north-south transitional zone of China.Analysis of its phenology is critical for comprehending the response of vegetation to climatic change.We r... The Qinba Mountains are climatically and ecologically recognized as the north-south transitional zone of China.Analysis of its phenology is critical for comprehending the response of vegetation to climatic change.We retrieved the start of spring phenology(SOS)of eight forest communities from the MODIS products and adopted it as an indicator for spring phenology.Trend analysis,partial correlation analysis,and GeoDetector were employed to reveal the spatio-temporal patterns and climatic drivers of SOS.The results indicated that the SOS presented an advance trend from 2001 to 2020,with a mean rate of−0.473 d yr^(−1).The SOS of most forests correlated negatively with air temperature(TEMP)and positively with precipitation(PRE),suggesting that rising TEMP and increasing PRE in spring would forward and delay SOS,respectively.The dominant factors influencing the sensitivity of SOS to climatic variables were altitude,forest type,and latitude,while the effects of slope and aspect were relatively minor.The response of SOS to climatic factors varied significantly in space and among forest communities,partly due to the influence of altitude,slope,and aspect. 展开更多
关键词 spring phenology climatic drivers altitude forest communities lag effect Qinba Mountains
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Spatiotemporal changes and interconnections between meteorological and hydrological droughts in China over past 34 years
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作者 Ke Zhang Zhi-lin Li +3 位作者 Wu-zhi Shi Ran Tao Xu Yang Yi-ming Huang 《Water Science and Engineering》 2025年第3期274-287,共14页
Understanding the evolution and lag effects of droughts is critical to effective drought warning and water resources management.However,due to limited hydrological data,few studies have examined hydrological droughts ... Understanding the evolution and lag effects of droughts is critical to effective drought warning and water resources management.However,due to limited hydrological data,few studies have examined hydrological droughts and their lag time from meteorological droughts at a daily scale.In this study,precipitation data were collected to calculate the standardized precipitation index(SPI),and runoff data simulated by the variable infiltration capacity(VIC)model were utilized to compute the standardized runoff index(SRI).The three-threshold run theory was used to identify drought characteristics in China.These drought characteristics were utilized to investigate spatiotemporal variations,seasonal trends,and temporal changes in areas affected by meteorological and hydrological droughts.Additionally,the interconnections and lag effects between meteorological and hydrological droughts were explored.The results indicated that(1)drought occurred during approximately 28%of the past 34 years in China;(2)drought conditions tended to worsen in autumn and weaken in winter;(3)drought-affected areas shifted from northwest to northeast and finally to southern China;and(4)the correlation between meteorological and hydrological droughts was lower in the northwest and higher in the southeast,with all correlation coefficients exceeding 0.7.The lag times between meteorological and hydrological droughts were longest(5 d)in the Yangtze River,Yellow River,and Hai River basins,and shortest(0 d)in the Tarim River Basin.This study provides a scientific basis for effective early warning of droughts. 展开更多
关键词 Drought characteristic Meteorological drought Hydrological drought Lag time VIC
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Rate-frequency dependent shear behavior of rough rock joint experiencing normal load oscillations
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作者 DANG Wen-gang LI Xing-ling +1 位作者 TAO Kang FU Jin-yang 《Journal of Central South University》 2025年第5期1873-1886,共14页
Dynamic disturbances certainly reduce shear strength of rock joints,yet the mechanism needs deeper explanation.We investigate the shear behavior of a rough basalt joint by conducting laboratory shear experiments.Const... Dynamic disturbances certainly reduce shear strength of rock joints,yet the mechanism needs deeper explanation.We investigate the shear behavior of a rough basalt joint by conducting laboratory shear experiments.Constant and superimposed oscillating normal loads are applied at the upper block.Meanwhile,the bottom block moves at a constant shear rate.We investigate the shear behavior by:1)altering the normal load oscillation frequency with a same shear rate,2)altering the shear rate with a same normal load oscillation frequency,and 3)altering the normal load oscillation frequency and shear rate simultaneously with a constant ratio.The results show that the oscillating normal load reduces the coefficient of friction(COF).The reduce degree of COF increases with higher shear rate,decreases when increasing normal load oscillation frequency,and keeps constant if the special ratio,v/f(shear rate divided by normal oscillation frequency),is constant.Moreover,we identify a time lag between peak normal load and peak shear load.And the lagging proportion increases with higher shear rate,and decreases with larger static COF.Our results imply that a lower creep rate with a higher normal load oscillation frequency easily destabilizes the creeping fault zones. 展开更多
关键词 normal load oscillation shear rate oscillation frequency friction reduction time lag
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Research on the hysteresis effect of topic related evolution for emerging trends prediction
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作者 Ziqiang Liu Haiyun Xu +1 位作者 Lixin Yue Zenghui Yue 《Journal of Data and Information Science》 2025年第3期52-77,共26页
Purpose:The study examines the synergy and hysteresis in the evolution of funding and its supported literature,depicts their temporal correlation mechanism,which aids in improving trend predictions.Design/methodology/... Purpose:The study examines the synergy and hysteresis in the evolution of funding and its supported literature,depicts their temporal correlation mechanism,which aids in improving trend predictions.Design/methodology/approach:The study uses the LDA model to identify topics in funding texts and supported papers.A cosine similarity algorithm was employed to estimate the nexus between topics and construct the topic evolution time series.Similarly,the hysteresis effect in topic evolution is analyzed based on topic popularity and content,leading to insights into their temporal correlation mechanism.Findings:The study finds that fund and sponsored paper topics exhibit strong collaboration with a noticeable lag in evolution.The fund topics significantly influence sponsored paper topics after a two-year lag.Moreover,the lag effect is inversely proportional to the topic’s similarity.Research limitations:We use the LDA model to determine the hysteresis effect in topic evolution despite its limitations in handling long-tail words and domain-specific vocabulary.Furthermore,the timing of the emergence of the focal topic in funds is undermined,affecting the findings.Practical implications:These findings enhance the accuracy and scientific validity of trend prediction.Estimating and identifying patterns can help technology managers anticipate future research hotspots,supporting informed decision-making and technology management.Originality/value:This study introduces a research framework to quantitatively and visually analyze the hysteresis effect,revealing the correlation and evolutionary patterns between fund research topics and their funded papers. 展开更多
关键词 Emerging trend prediction Topic association Topic evolution Hysteresis effect Lag period
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Anticipating Lag Synchronization Based on Machine Learning
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作者 WU Yongqing BAO Xingxing 《数学理论与应用》 2025年第1期115-126,共12页
This paper propose a comprehensive data-driven prediction framework based on machine learning methods to investigate the lag synchronization phenomenon in coupled chaotic systems,particularly in cases where accurate m... This paper propose a comprehensive data-driven prediction framework based on machine learning methods to investigate the lag synchronization phenomenon in coupled chaotic systems,particularly in cases where accurate mathematical models are challenging to establish or where system equations remain unknown.The Long Short-Term Memory(LSTM)neural network is trained using time series acquired from the desynchronization system states,subsequently predicting the lag synchronization transition.In the experiments,we focus on the Lorenz system with time-varying delayed coupling,studying the effects of coupling coefficients and time delays on lag synchronization,respectively.The results indicate that with appropriate training,the machine learning model can adeptly predict the lag synchronization occurrence and transition.This study not only enhances our comprehension of complex network synchronization behaviors but also underscores the potential and practical applications of machine learning in exploring nonlinear dynamic systems. 展开更多
关键词 Coupled chaotic system LSTM neural network Anticipating synchronization Lag synchronization
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Optimized Lagged Multiple Linear Regression Model for MJO Prediction:Considering the Surface and Subsurface Oceanic Processes over the Maritime Continent
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作者 LU Kecheng LI Yiran +1 位作者 HU Haibo WANG Ziyi 《Journal of Ocean University of China》 2025年第4期840-850,共11页
The Madden-Julian Oscillation(MJO)is a key atmospheric component connecting global weather and climate.It func-tions as a primary source for subseasonal forecasts.Previous studies have highlighted the vital impact of ... The Madden-Julian Oscillation(MJO)is a key atmospheric component connecting global weather and climate.It func-tions as a primary source for subseasonal forecasts.Previous studies have highlighted the vital impact of oceanic processes on MJO propagation.However,few existing MJO prediction approaches adequately consider these factors.This study determines the critical region for the oceanic processes affecting MJO propagation by utilizing 22-year Climate Forecast System Reanalysis data.By intro-ducing surface and subsurface oceanic temperature within this critical region into a lagged multiple linear regression model,the MJO forecasting skill is considerably optimized.This optimization leads to a 12 h enhancement in the forecasting skill of the first principal component and efficiently decreases prediction errors for the total predictions.Further analysis suggests that,during the years in which MJO events propagate across the Maritime Continent over a more southerly path,the optimized statistical forecasting model obtains better improvements in MJO prediction. 展开更多
关键词 Madden-Julian Oscillation statistical forecasting Maritime Continent oceanic processes lagged multiple linear re-gression model
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Spatial Modeling of COVID-19 Occurrence and Vaccination Rate across Counties in Ohio State from Jan. 2020 to April 2023
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作者 Olawale Oluwafemi Oluwaseun Ibukun +3 位作者 Yaw Kwarteng Kehinde Adebowale Yahaya Danjuma Samson Mela 《Journal of Geographic Information System》 2025年第1期80-96,共17页
The study aims to investigate county-level variations of the COVID-19 disease and vaccination rate. The COVID-19 data was acquired from usafact.org, and the vaccination records were acquired from the Ohio vaccination ... The study aims to investigate county-level variations of the COVID-19 disease and vaccination rate. The COVID-19 data was acquired from usafact.org, and the vaccination records were acquired from the Ohio vaccination tracker dashboard. GIS-based exploratory analysis was conducted to select four variables (poverty, black race, population density, and vaccination) to explain COVID-19 occurrence during the study period. Consequently, spatial statistical techniques such as Moran’s I, Hot Spot Analysis, Spatial Lag Model (SLM), and Spatial Error Model (SEM) were used to explain the COVID-19 occurrence and vaccination rate across the 88 counties in Ohio. The result of the Local Moran’s I analysis reveals that the epicenters of COVID-19 and vaccination followed the same patterns. Indeed, counties like Summit, Franklin, Fairfield, Hamilton, and Medina were categorized as epicenters for both COVID-19 occurrence and vaccination rate. The SEM seems to be the best model for both COVID-19 and vaccination rates, with R2 values of 0.68 and 0.70, respectively. The GWR analysis proves to be better than Ordinary Least Squares (OLS), and the distribution of R2 in the GWR is uneven throughout the study area for both COVID-19 cases and vaccinations. Some counties have a high R2 of up to 0.70 for both COVID-19 cases and vaccinations. The outcomes of the regression analyses show that the SEM models can explain 68% - 70% of COVID-19 cases and vaccination across the entire counties within the study period. COVID-19 cases and vaccination rates exhibited significant positive associations with black race and poverty throughout the study area. 展开更多
关键词 COVID-19 Prevalence COVID-19 Vaccination OHIO Spatial Lag Model Spatial Error Model
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Mutual Relationship between Grip Strength and Cognitive Function in Chinese Middle-Aged and Elderly People over 10 Years:A Cross-Lagged Panel Analysis
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作者 Jiaqi Wang Ye Ruan +5 位作者 Yanfei Guo Shuangyuan Sun Anli Jiang Yujun Dong Yan Shi Fan Wu 《Biomedical and Environmental Sciences》 2025年第10期1308-1313,共6页
In 2019,China had over 13.14 million dementia cases,with incidence rates of(56.47–207.08)/100,000[1].Early cognitive impairment—a key dementia symptom—reduces quality of life,increases care dependence,and lowers su... In 2019,China had over 13.14 million dementia cases,with incidence rates of(56.47–207.08)/100,000[1].Early cognitive impairment—a key dementia symptom—reduces quality of life,increases care dependence,and lowers survival in older adults[2].A decline in physical function can also be observed in older adults with increasing age.Grip strength has been shown to be a marker of overall physiological function in older adults. 展开更多
关键词 decline physical function physiological function cognitive function cognitive impairment Chinese middle aged elderly grip strength cross lagged panel analysis DEMENTIA
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阿尔茨海默病中免疫检查位点LAG3的研究
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作者 孟凌云 段冉 陈静 《中国免疫学杂志》 CAS CSCD 北大核心 2024年第2期273-277,共5页
目的:探讨免疫检查位点基因LAG3在阿尔茨海默病(AD)中的作用和意义。方法:对GEO(https://www.ncbi.nlm.nih.gov/geo/)数据库中AD患者脑组织测序数据集GSE48350和血液测序数据集GSE140829进行分析,采用CIBERSORT计算脑组织中的免疫细胞评... 目的:探讨免疫检查位点基因LAG3在阿尔茨海默病(AD)中的作用和意义。方法:对GEO(https://www.ncbi.nlm.nih.gov/geo/)数据库中AD患者脑组织测序数据集GSE48350和血液测序数据集GSE140829进行分析,采用CIBERSORT计算脑组织中的免疫细胞评分,并观察LAG3的表达量变化;使用人源Aβ1-42淀粉样蛋白诱导小鼠海马神经元细胞系HT22作为AD细胞模型,RT-PCR检测LAG3的表达量变化。通过GSEA分析研究与LAG3直接相关的通路,String网站构建PPI网络,发现与LAG3相互作用的基因,最后通过KEGG和GO分析这些基因的生物学功能。结果:AD患者脑组织中免疫细胞与健康人存在较大差异,AD患者脑组织和血液中LAG3水平均高于健康人,AD细胞模型中LAG3的表达量也增高。结论:AD脑组织中免疫细胞构成与健康人存在差异,AD脑组织中未激活的记忆CD4^(+)T细胞和单核细胞的比例较高,激活的NK细胞偏低,且免疫检查位点LAG3的表达量增高,其可能与CD4细胞有关,也与免疫细胞的活化和调节存在联系。 展开更多
关键词 阿尔茨海默病 LAG3 免疫检查位点 CD4 免疫细胞评分
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重组真核细胞抗原免疫小鼠制备抗人LAG3单克隆抗体及其鉴定 被引量:1
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作者 李欣悦 蔡秀 金月 《细胞与分子免疫学杂志》 CSCD 北大核心 2024年第12期1110-1114,共5页
目的制备小鼠抗人淋巴细胞活化基因3(LAG3)单克隆抗体(mAb)并进行抗体免疫学鉴定。方法以构建的稳定表达人LAG3胞外区和跨膜区的小鼠3T3单克隆细胞(LAG3-mLumin-3T3)免疫BALB/c小鼠,流式细胞术和免疫荧光法检测小鼠血清中是否含有抗人L... 目的制备小鼠抗人淋巴细胞活化基因3(LAG3)单克隆抗体(mAb)并进行抗体免疫学鉴定。方法以构建的稳定表达人LAG3胞外区和跨膜区的小鼠3T3单克隆细胞(LAG3-mLumin-3T3)免疫BALB/c小鼠,流式细胞术和免疫荧光法检测小鼠血清中是否含有抗人LAG3抗体。用SP2/0细胞皮下注射BALB/c小鼠形成实体骨髓瘤,体外分离的小鼠骨髓瘤细胞与免疫小鼠的脾细胞融合建立杂交瘤,用有限细胞稀释法分离出杂交瘤单克隆细胞。收集杂交瘤单克隆细胞培养液,流式细胞术检测细胞分泌LAG3 mAb。构建的表达LAG3胞外区4个独立结构域的3T3细胞株(LAG3-胞外结构域1/-2/-3/-4-3T3)用于流式细胞术区分mAb结合抗原表位。流式细胞术检测LAG3 mAb与活化状态下的人外周血单核细胞(PBMC)共同培养前后细胞LAG3表达。结果重组真核细胞抗原免疫后小鼠能产生特异性抗人LAG3抗体。通过杂交瘤融合获得的杂交瘤单克隆细胞能够分泌小鼠抗人LAG3 mAb;不同单克隆细胞株分泌的mAb识别的LAG3抗原结构域存在差异。结论成功制备小鼠抗人LAG3 mAb,不同杂交瘤细胞克隆分泌的mAb识别表位不同,为进一步研究LAG3生物学特性及肿瘤治疗阻断抗体的研发奠定基础。 展开更多
关键词 淋巴细胞活化基因3(LAG3) 真核细胞抗原 活体骨髓瘤细胞 杂交瘤 单克隆抗体(mAb)
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Shear mechanical properties and frictional sliding responses of rough joint surfaces under dynamic normal displacement conditions 被引量:1
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作者 ZHU Qiang YIN Qian +9 位作者 TAO Zhi-gang HE Man-chao ZHENG Bo-wen JING Hong-wen REN Shu-lin ZHANG Qiang MENG Bo BAI Dong-feng WU Sai-sai WU Jiang-yu 《Journal of Central South University》 SCIE EI CAS CSCD 2024年第7期2393-2410,共18页
A comprehensive understanding of the dynamic frictional characteristics in rock joints under high normal load and strong confinement is essential for ensuring the safety of deep engineering construction and mitigating... A comprehensive understanding of the dynamic frictional characteristics in rock joints under high normal load and strong confinement is essential for ensuring the safety of deep engineering construction and mitigating geological disasters.This study conducted shear experiments on rough rock joints under displacement-controlled dynamic normal loads,investigating the shear behaviors of joints across varying initial normal loads,normal loading frequencies,and normal loading amplitudes.Experimental results showed that the peak/valley shear force values increased with initial normal loads and normal loading frequencies but showed an initial increase followed by a decrease with normal loading amplitudes.Dynamic normal loading can either increase or decrease shear strength,while this study demonstrates that higher frequencies lead to enhanced friction.Increased initial normal loading and normal loading frequency result in a gradual decrease in joint roughness coefficient(JRC)values of joint surfaces after shearing.Positive correlations existed between frictional energy dissipation and peak shear forces,while post-shear joint surface roughness exhibited a negative correlation with peak shear forces through linear regression analysis.This study contributes to a better understanding of the sliding responses and shear mechanical characteristics of rock joints under dynamic disturbances. 展开更多
关键词 dynamic normal displacement shear strength variations phase lag phenomenon STICK-SLIP
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Cross-Correlation between Global Temperature and Atmospheric CO2 with a Temperature-Leading Time Lag 被引量:1
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作者 Masaharu Nishioka 《Atmospheric and Climate Sciences》 2024年第4期484-494,共11页
The temperature change and rate of CO2 change are correlated with a time lag, as reported in a previous paper. The correlation was investigated by calculating a correlation coefficient r of these changes for selected ... The temperature change and rate of CO2 change are correlated with a time lag, as reported in a previous paper. The correlation was investigated by calculating a correlation coefficient r of these changes for selected ENSO events in this study. Annual periodical increases and decreases in the CO2 concentration were considered, with a regular pattern of minimum values in August and maximum values in May each year. An increased deviation in CO2 and temperature was found in response to the occurrence of El Niño, but the increase in CO2 lagged behind the change in temperature by 5 months. This pattern was not observed for La Niña events. An increase in global CO2 emissions and a subsequent increase in global temperature proposed by IPCC were not observed, but an increase in global temperature, an increase in soil respiration, and a subsequent increase in global CO2 emissions were noticed. This natural process can be clearly detected during periods of increasing temperature specifically during El Niño events. The results cast strong doubts that anthropogenic CO2 is the cause of global warming. 展开更多
关键词 Global Warming Thermally-Induced CO2 Soil Respiration Cross-Correlation Coefficient Time Lag El Niño
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