Malignant tumours always threaten human health.For tumour diagnosis,positron emission tomography(PET)is the most sensitive and advanced imaging technique by radiotracers,such as radioactive^(18)F,^(11)C,^(64)Cu,^(68)G...Malignant tumours always threaten human health.For tumour diagnosis,positron emission tomography(PET)is the most sensitive and advanced imaging technique by radiotracers,such as radioactive^(18)F,^(11)C,^(64)Cu,^(68)Ga,and^(89)Zr.Among the radiotracers,the radioactive^(18)F-labelled chemical agent as PET probes plays a predominant role in monitoring,detecting,treating,and predicting tumours due to its perfect half-life.In this paper,the^(18)F-labelled chemical materials as PET probes are systematically summarized.First,we introduce various radionuclides of PET and elaborate on the mechanism of PET imaging.It highlights the^(18)F-labelled chemical agents used as PET probes,including[^(18)F]-2-deoxy-2-[^(18)F]fluoro-D-glucose([^(18)F]-FDG),^(18)F-labelled amino acids,^(18)F-labelled nucleic acids,^(18)F-labelled receptors,^(18)F-labelled reporter genes,and^(18)F-labelled hypoxia agents.In addition,some PET probes with metal as a supplementary element are introduced briefly.Meanwhile,the^(18)F-labelled nanoparticles for the PET probe and the multi-modality imaging probe are summarized in detail.The approach and strategies for the fabrication of^(18)F-labelled PET probes are also described briefly.The future development of the PET probe is also prospected.The development and application of^(18)F-labelled PET probes will expand our knowledge and shed light on the diagnosis and theranostics of tumours.展开更多
In 2012, Ponraj et al. defined a concept of k-product cordial labeling as follows: Let f be a map from V(G)to { 0,1,⋯,k−1 }where k is an integer, 1≤k≤| V(G) |. For each edge uvassign the label f(u)f(v)(modk). f is c...In 2012, Ponraj et al. defined a concept of k-product cordial labeling as follows: Let f be a map from V(G)to { 0,1,⋯,k−1 }where k is an integer, 1≤k≤| V(G) |. For each edge uvassign the label f(u)f(v)(modk). f is called a k-product cordial labeling if | vf(i)−vf(j) |≤1, and | ef(i)−ef(j) |≤1, i,j∈{ 0,1,⋯,k−1 }, where vf(x)and ef(x)denote the number of vertices and edges respectively labeled with x (x=0,1,⋯,k−1). Motivated by this concept, we further studied and established that several families of graphs admit k-product cordial labeling. In this paper, we show that the path graphs Pnadmit k-product cordial labeling.展开更多
Semantic segmentation is a core task in computer vision that allows AI models to interact and understand their surrounding environment. Similarly to how humans subconsciously segment scenes, this ability is crucial fo...Semantic segmentation is a core task in computer vision that allows AI models to interact and understand their surrounding environment. Similarly to how humans subconsciously segment scenes, this ability is crucial for scene understanding. However, a challenge many semantic learning models face is the lack of data. Existing video datasets are limited to short, low-resolution videos that are not representative of real-world examples. Thus, one of our key contributions is a customized semantic segmentation version of the Walking Tours Dataset that features hour-long, high-resolution, real-world data from tours of different cities. Additionally, we evaluate the performance of open-vocabulary, semantic model OpenSeeD on our own custom dataset and discuss future implications.展开更多
Site-specific protein labeling plays important roles in drug discovery and illuminating biological processes at the molecular level.However,it is challenging to label proteins with high specificity while not affecting...Site-specific protein labeling plays important roles in drug discovery and illuminating biological processes at the molecular level.However,it is challenging to label proteins with high specificity while not affecting their structures and biochemical activities.Over the last few years,a variety of promising strategies have been devised that address these challenges including those that involve introduction of small-size peptide tags or unnatural amino acids(UAAs),chemical labeling of specific protein residues,and affinity-driven labeling.This review summarizes recent developments made in the area of site-specific protein labeling utilizing genetically encoding-and chemical-based methods,and discusses future issues that need to be addressed by researchers in this field.展开更多
The China National Institute of Standardization(CNIS)held the Academic Meeting on 20th Anniversary of China Energy Label in Beijing on June 27.The event took place during the 35th National Energy Conservation Publicit...The China National Institute of Standardization(CNIS)held the Academic Meeting on 20th Anniversary of China Energy Label in Beijing on June 27.The event took place during the 35th National Energy Conservation Publicity Week,which ran from June 23 to 29.展开更多
The distance distributions between two site-specifically anchored spin labels in a protein,measured by pulsed electron-electron double resonance(PELDOR or DEER),provide rich sources of structural and conformational re...The distance distributions between two site-specifically anchored spin labels in a protein,measured by pulsed electron-electron double resonance(PELDOR or DEER),provide rich sources of structural and conformational restraints on the proteins or their complexes.The rigid connection of the nitroxide spin label to the protein improves the accuracy and precision of distance measurement.We report a new spin labelling approach by formation of thioester bond between nitroxide(NO)spin label,NOAI(NO spin labels activated by acetylimidazole),and a protein thiol,and this spin labeling method has demonstrated high performance in DEER distance measurement on proteins.The results showed that NOAI has shorter connection to the protein ligation site than 2,2,5,5-tetramethyl-pyrroline-1-oxyl methanethiosulfonate(MTSL)and 3-maleimido-proxyl(M-Prox)in the respective protein conjugate and produces narrower distance distributions for the tested proteins including ubiquitin(Ub),immunoglobulin-binding b1 domain of streptococcal protein G(GB1),and second mitochondria-derived activator of caspases(Smac).The NOAI protein conjugate connected by a thioester bond is resistant to reducing reagent and offers highfidelity DEER distance measurements in cell lysates.展开更多
This study summarizes the examination data of registration labels for ordinary cosmetics in Beijing from May 2021 to April 2024.It analyzes and categorizes the issues identified during label evaluations,explores the u...This study summarizes the examination data of registration labels for ordinary cosmetics in Beijing from May 2021 to April 2024.It analyzes and categorizes the issues identified during label evaluations,explores the underlying causes,and proposes regulatory countermeasures and recommendations for registrants,regulatory authorities,and social organizations.The objective is to offer practical insights and regulatory guidance to support the enhancement of cosmetic registration and regulatory standards.展开更多
Digital twin technology is revolutionizing personalized healthcare by creating dynamic virtual replicas of individual patients.This paper presents a novel multi-modal architecture leveraging digital twins to enhance p...Digital twin technology is revolutionizing personalized healthcare by creating dynamic virtual replicas of individual patients.This paper presents a novel multi-modal architecture leveraging digital twins to enhance precision in predictive diagnostics and treatment planning of phoneme labeling.By integrating real-time images,electronic health records,and genomic information,the system enables personalized simulations for disease progression modeling,treatment response prediction,and preventive care strategies.In dysarthric speech,which is characterized by articulation imprecision,temporal misalignments,and phoneme distortions,existing models struggle to capture these irregularities.Traditional approaches,often relying solely on audio features,fail to address the full complexity of phoneme variations,leading to increased phoneme error rates(PER)and word error rates(WER).To overcome these challenges,we propose a novel multi-modal architecture that integrates both audio and articulatory data through a combination of Temporal Convolutional Networks(TCNs),Graph Convolutional Networks(GCNs),Transformer Encoders,and a cross-modal attention mechanism.The audio branch of the model utilizes TCNs and Transformer Encoders to capture both short-and long-term dependencies in the audio signal,while the articulatory branch leverages GCNs to model spatial relationships between articulators,such as the lips,jaw,and tongue,allowing the model to detect subtle articulatory imprecisions.A cross-modal attention mechanism fuses the encoded audio and articulatory features,enabling dynamic adjustment of the model’s focus depending on input quality,which significantly improves phoneme labeling accuracy.The proposed model consistently outperforms existing methods,achieving lower Phoneme Error Rates(PER),Word Error Rates(WER),and Articulatory Feature Misclassification Rates(AFMR).Specifically,across all datasets,the model achieves an average PER of 13.43%,an average WER of 21.67%,and an average AFMR of 12.73%.By capturing both the acoustic and articulatory intricacies of speech,this comprehensive approach not only improves phoneme labeling precision but also marks substantial progress in speech recognition technology for individuals with dysarthria.展开更多
Since the 1970s,a series of international and national sources have supported the principle of accessibility,which slowly has become a statuary norm and a legislative obligation.Each country has implemented accessibil...Since the 1970s,a series of international and national sources have supported the principle of accessibility,which slowly has become a statuary norm and a legislative obligation.Each country has implemented accessibility through a singular policy.But in addition to the accessibility of a place or an activity,to inform about what is accessible is very important as well,and has not really taken off.Indeed,for disabled people,the difficulty lies not only with access to places and the use of resources,but also with the visibility of these resources.This means that information concerning accessibility has to be disclosed and provided effectively to disabled people,those involved with them and the relevant institutions.In different countries all over the world,many labels and pictograms have been created for this purpose and give information relating to accessibility.Using a socio-historical approach,we will present and analyze the different types of icons,symbols,pictograms and labels that have been put in place around the world and in France:what are they used for and for whom are they made?We will show that they are pointers which firstly reflect the diversity and range within the target group concerned by accessibility,and secondly the evolution of accessibility as a dynamic and ecological principle.展开更多
This paper is concerned with design-ing symbol labeling for a low-density parity-check(LDPC)-coded delayed bit-interleaved coded modu-lation(DBICM)scheme in a two-way relay channel(TWRC).We first present some properti...This paper is concerned with design-ing symbol labeling for a low-density parity-check(LDPC)-coded delayed bit-interleaved coded modu-lation(DBICM)scheme in a two-way relay channel(TWRC).We first present some properties of symbol labeling within a phase shift keying(PSK)modula-tion.These properties reduce the candidate labeling search space.Based on this search space,we take DBICM capacity as the cost function and propose a general method for optimizing symbol labeling by em-ploying the differential evolution algorithm.Numeri-cal results show that our labeling obtains a signal-to-noise ratio(SNR)gain up to 0.45 dB with respect to Gray labeling.展开更多
Acute lung injury(ALI)is a serious clinical condition with a high mortality rate.Oxidative stress and inflammatory responses play pivotal roles in the pathogenesis of ALI.ONOO^(−)is a key mediator that exacerbates oxi...Acute lung injury(ALI)is a serious clinical condition with a high mortality rate.Oxidative stress and inflammatory responses play pivotal roles in the pathogenesis of ALI.ONOO^(−)is a key mediator that exacerbates oxidative damage and microvascular permeability in ALI.Accurate detection of ONOO^(−)would facilitate early diagnosis and intervention in ALI.Near-infrared fluorescence(NIRF)probes offer new solutions due to their sensitivity,depth of tissue penetration,and imaging capabilities.However,the developed ONOO^(−)fluorescent probes face problems such as interference from other reactive oxygen species and easy intracellular diffusion.To address these issues,we introduced an innovative self-immobilizing NIRF probe,DCI2F-OTf,which was capable of monitoring ONOO^(−)in vitro and in vivo.Importantly,leveraging the high reactivity of the methylene quinone(QM)intermediate,DCI2F-OTf was able to covalently label proteins in the presence of ONOO^(−),enabling in situ imaging.In mice models of ALI,DCI2F-OTf enabled real-time imaging of ONOO^(−)levels and found that ONOO^(−)was tightly correlated with the progression of ALI.Our findings demonstrated that DCI2F-OTf was a promising chemical tool for the detection of ONOO^(−),which could help to gain insight into the pathogenesis of ALI and monitor treatment efficacy.展开更多
Many existing immune detection algorithms rely on a large volume of labeled self-training samples,which are often difficult to obtain in practical scenarios,thus limiting the training of detection models.Furthermore,n...Many existing immune detection algorithms rely on a large volume of labeled self-training samples,which are often difficult to obtain in practical scenarios,thus limiting the training of detection models.Furthermore,noise inherent in the samples can substantially degrade the detection accuracy of these algorithms.To overcome these challenges,we propose an immune generation algorithm that leverages clustering and a rebound mechanism for label propagation(LP-CRI).The dataset is randomly partitioned into multiple subsets,each of which undergoes clustering followed by label propagation and evaluation.The rebound mechanism assesses the model’s performance after propagation and determines whether to revert to its previous state,initiating a subsequent round of propagation to ensure stable and effective training.Experimental results demonstrate that the proposed method is both computationally efficient and easy to train,significantly enhancing detector performance and outperforming traditional immune detection algorithms.展开更多
Intelligent fault diagnosis technology plays an indispensable role in ensuring the safety,stability,and efficiency of railway operations.However,existing studies have the following limitations.1)They are typical black-...Intelligent fault diagnosis technology plays an indispensable role in ensuring the safety,stability,and efficiency of railway operations.However,existing studies have the following limitations.1)They are typical black-box models that lacks interpretability as well as they fuse features by simply stacking them,overlooking the discrepancies in the importance of different features,which reduces the credibility and diagnosis accuracy of the models.2)They ignore the effects of potentially mistaken labels in the training datasets disrupting the ability of the models to learn the true data distribution,which degrades the generalization performance of intelligent diagnosis models,especially when the training samples are limited.To address the above items,an interpretable few-shot framework for fault diagnosis with noisy labels is proposed for train transmission systems.In the proposed framework,a feature extractor is constructed by stacked frequency band focus modules,which can capture signal features in different frequency bands and further adaptively concentrate on the features corresponding to the potential fault characteristic frequency.Then,according to prototypical network,a novel metric-based classifier is developed that is tolerant to mislabeled support samples in the case of limited samples.Besides,a new loss function is designed to decrease the impact of label mistakes in query datasets.Finally,fault simulation experiments of subway train transmission systems are designed and conducted,and the effectiveness as well as superiority of the proposed method are proved by ablation experiments and comparison with the existing methods.展开更多
This paper addresses the verification of strong currentstate opacity with respect to real-time observations generated from a discrete-event system that is modeled with time labeled Petri nets. The standard current-sta...This paper addresses the verification of strong currentstate opacity with respect to real-time observations generated from a discrete-event system that is modeled with time labeled Petri nets. The standard current-state opacity cannot completely characterize higher-level security. To ensure the higher-level security requirements of a time-dependent system, we propose a strong version of opacity known as strong current-state opacity. For any path(state-event sequence with time information)π derived from a real-time observation that ends at a secret state, the strong current-state opacity of the real-time observation signifies that there is a non-secret path with the same real-time observation as π. We propose general and non-secret state class graphs, which characterize the general and non-secret states of time-dependent systems, respectively. To capture the observable behavior of non-secret states, a non-secret observer is proposed.Finally, we develop a structure called a real-time concurrent verifier to verify the strong current-state opacity of time labeled Petri nets. This approach is efficient since the real-time concurrent verifier can be constructed by solving a certain number of linear programming problems.展开更多
To extract and display the significant information of combat systems,this paper introduces the methodology of functional cartography into combat networks and proposes an integrated framework named“functional cartogra...To extract and display the significant information of combat systems,this paper introduces the methodology of functional cartography into combat networks and proposes an integrated framework named“functional cartography of heterogeneous combat networks based on the operational chain”(FCBOC).In this framework,a functional module detection algorithm named operational chain-based label propagation algorithm(OCLPA),which considers the cooperation and interactions among combat entities and can thus naturally tackle network heterogeneity,is proposed to identify the functional modules of the network.Then,the nodes and their modules are classified into different roles according to their properties.A case study shows that FCBOC can provide a simplified description of disorderly information of combat networks and enable us to identify their functional and structural network characteristics.The results provide useful information to help commanders make precise and accurate decisions regarding the protection,disintegration or optimization of combat networks.Three algorithms are also compared with OCLPA to show that FCBOC can most effectively find functional modules with practical meaning.展开更多
Background:Rabies virus(RABV)-derived neuronal tracing tools are extensively applied in retrograde tracing due to their strict retrograde transsynaptic transfer property and low neurotoxicity.However,the RABV infectio...Background:Rabies virus(RABV)-derived neuronal tracing tools are extensively applied in retrograde tracing due to their strict retrograde transsynaptic transfer property and low neurotoxicity.However,the RABV infection and expression of fluorescence products would be gradually cleared while the infected neurons still survive,a phenomenon known as non-cytolytic immune clearance(NCLIC).This phenomenon introduced the risk of fluorescence loss and led to the omission of a subset of neurons that should be labeled,thereby interfering in the analysis of tracing results.Methods:To compensate for the fluorescence loss problem,in this study,we developed a novel marker footprints(MF)mouse,involving a Cre recombinase-dependent red fluorescent reporter system and systemic expression of glycoprotein(G)and ASLV-A receptor(TVA).Using this mouse model combined with the well-developed RABV-EnvA-ΔG-GFP-Cre viral tool,we developed a novel green-to-red spectral labeling strategy.Results:Neurons in the MF mouse could be co-labeled with green fluorescence from the very quick expression of the viral tool and with red fluorescence from the relatively slow expression of the neuron itself,so neurons undergoing NCLIC with green fluorescence loss could be relabeled red.Furthermore,newly infected neurons could be labeled green and other neurons could be labeled yellow due to the temporal expression difference between the two fluorescent proteins.Conclusions:This is the first polysynaptic retrograde tracing labeling strategy that could label neurons using spectral fluorescence colors with only one injection of the viral tool,enabling its application in recognizing the labeling sequence of neurons in brain regions and enhancing the spatiotemporal resolution of neuronal tracing.展开更多
Nanoclays have large specific surface area,good adsorption properties,and biocompatibility that have great potential for drug delivery applications[1].Evaluating the in vivo metabolic pathways of nanoclays can help to...Nanoclays have large specific surface area,good adsorption properties,and biocompatibility that have great potential for drug delivery applications[1].Evaluating the in vivo metabolic pathways of nanoclays can help to understand their pharmacodynamic sites and the toxicological effects caused by their in vivo retention time[2].展开更多
The unsupervised vehicle re-identification task aims at identifying specific vehicles in surveillance videos without utilizing annotation information.Due to the higher similarity in appearance between vehicles compare...The unsupervised vehicle re-identification task aims at identifying specific vehicles in surveillance videos without utilizing annotation information.Due to the higher similarity in appearance between vehicles compared to pedestrians,pseudo-labels generated through clustering are ineffective in mitigating the impact of noise,and the feature distance between inter-class and intra-class has not been adequately improved.To address the aforementioned issues,we design a dual contrastive learning method based on knowledge distillation.During each iteration,we utilize a teacher model to randomly partition the entire dataset into two sub-domains based on clustering pseudo-label categories.By conducting contrastive learning between the two student models,we extract more discernible vehicle identity cues to improve the problem of imbalanced data distribution.Subsequently,we propose a context-aware pseudo label refinement strategy that leverages contextual features by progressively associating granularity information from different bottleneck blocks.To produce more trustworthy pseudo-labels and lessen noise interference during the clustering process,the context-aware scores are obtained by calculating the similarity between global features and contextual ones,which are subsequently added to the pseudo-label encoding process.The proposed method has achieved excellent performance in overcoming label noise and optimizing data distribution through extensive experimental results on publicly available datasets.展开更多
Self-labeling protein (SLP) tags, such as HaloTag, have gained considerable interest as advanced tools for live cell labeling. However, the chloroalkane-based substrates that can be directly used for protein labeling ...Self-labeling protein (SLP) tags, such as HaloTag, have gained considerable interest as advanced tools for live cell labeling. However, the chloroalkane-based substrates that can be directly used for protein labeling are limited. Here, we report two bioorthogonal small molecule linkers, chloroalkane-tetrazine (CA-Tz) and chloroalkane-azide (CA-N3), which can penetrate cell membranes and facilitate click chemistry-based labeling in live cells. We compare their labeling capability using two clickable silicon rhodamine dyes (SiR-PEG_(3)-TCO and SiR-PEG_(4)-DBCO). Confocal imaging results demonstrate that using CA-Tz and SiR-PEG_(3)-TCO dye exhibits superior intracellular labeling with low nonspecific signals. We subsequently compared the photostability of SiR dyes with that of green fluorescent proteins (mEmerald). Total internal reflection fluorescence (TIRF) imaging indicates that SiR dyes exhibit superior photostability under identical excitation conditions, making them suitable for long-term cell imaging. Furthermore, SiR dyes labeling also shows high structure retention for the fourth-order super-resolution optical fluctuation imaging (SOFI) compared to fluorescent proteins. This study presents clickable HaloTag linkers as effective tools for live cell labeling and imaging, highlighting the high-quality labeling of chloroalkane linkers and clickable dyes for live cell imaging.展开更多
文摘Malignant tumours always threaten human health.For tumour diagnosis,positron emission tomography(PET)is the most sensitive and advanced imaging technique by radiotracers,such as radioactive^(18)F,^(11)C,^(64)Cu,^(68)Ga,and^(89)Zr.Among the radiotracers,the radioactive^(18)F-labelled chemical agent as PET probes plays a predominant role in monitoring,detecting,treating,and predicting tumours due to its perfect half-life.In this paper,the^(18)F-labelled chemical materials as PET probes are systematically summarized.First,we introduce various radionuclides of PET and elaborate on the mechanism of PET imaging.It highlights the^(18)F-labelled chemical agents used as PET probes,including[^(18)F]-2-deoxy-2-[^(18)F]fluoro-D-glucose([^(18)F]-FDG),^(18)F-labelled amino acids,^(18)F-labelled nucleic acids,^(18)F-labelled receptors,^(18)F-labelled reporter genes,and^(18)F-labelled hypoxia agents.In addition,some PET probes with metal as a supplementary element are introduced briefly.Meanwhile,the^(18)F-labelled nanoparticles for the PET probe and the multi-modality imaging probe are summarized in detail.The approach and strategies for the fabrication of^(18)F-labelled PET probes are also described briefly.The future development of the PET probe is also prospected.The development and application of^(18)F-labelled PET probes will expand our knowledge and shed light on the diagnosis and theranostics of tumours.
文摘In 2012, Ponraj et al. defined a concept of k-product cordial labeling as follows: Let f be a map from V(G)to { 0,1,⋯,k−1 }where k is an integer, 1≤k≤| V(G) |. For each edge uvassign the label f(u)f(v)(modk). f is called a k-product cordial labeling if | vf(i)−vf(j) |≤1, and | ef(i)−ef(j) |≤1, i,j∈{ 0,1,⋯,k−1 }, where vf(x)and ef(x)denote the number of vertices and edges respectively labeled with x (x=0,1,⋯,k−1). Motivated by this concept, we further studied and established that several families of graphs admit k-product cordial labeling. In this paper, we show that the path graphs Pnadmit k-product cordial labeling.
文摘Semantic segmentation is a core task in computer vision that allows AI models to interact and understand their surrounding environment. Similarly to how humans subconsciously segment scenes, this ability is crucial for scene understanding. However, a challenge many semantic learning models face is the lack of data. Existing video datasets are limited to short, low-resolution videos that are not representative of real-world examples. Thus, one of our key contributions is a customized semantic segmentation version of the Walking Tours Dataset that features hour-long, high-resolution, real-world data from tours of different cities. Additionally, we evaluate the performance of open-vocabulary, semantic model OpenSeeD on our own custom dataset and discuss future implications.
基金supported by the National Key R&D Program of China(No.2021YFC2103600)the National Natural Science Foundation of China(Nos.22278224,22478191)+1 种基金the Project of Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the State Key Laboratory of Materials Oriented Chemical Engineering(No.KL21-08)。
文摘Site-specific protein labeling plays important roles in drug discovery and illuminating biological processes at the molecular level.However,it is challenging to label proteins with high specificity while not affecting their structures and biochemical activities.Over the last few years,a variety of promising strategies have been devised that address these challenges including those that involve introduction of small-size peptide tags or unnatural amino acids(UAAs),chemical labeling of specific protein residues,and affinity-driven labeling.This review summarizes recent developments made in the area of site-specific protein labeling utilizing genetically encoding-and chemical-based methods,and discusses future issues that need to be addressed by researchers in this field.
文摘The China National Institute of Standardization(CNIS)held the Academic Meeting on 20th Anniversary of China Energy Label in Beijing on June 27.The event took place during the 35th National Energy Conservation Publicity Week,which ran from June 23 to 29.
基金supported by National Natural Science Foundation of China(22161142018,21991081,22177056,and 22174074)the Ministry of Science and Technology of China(2021YFA1600304).
文摘The distance distributions between two site-specifically anchored spin labels in a protein,measured by pulsed electron-electron double resonance(PELDOR or DEER),provide rich sources of structural and conformational restraints on the proteins or their complexes.The rigid connection of the nitroxide spin label to the protein improves the accuracy and precision of distance measurement.We report a new spin labelling approach by formation of thioester bond between nitroxide(NO)spin label,NOAI(NO spin labels activated by acetylimidazole),and a protein thiol,and this spin labeling method has demonstrated high performance in DEER distance measurement on proteins.The results showed that NOAI has shorter connection to the protein ligation site than 2,2,5,5-tetramethyl-pyrroline-1-oxyl methanethiosulfonate(MTSL)and 3-maleimido-proxyl(M-Prox)in the respective protein conjugate and produces narrower distance distributions for the tested proteins including ubiquitin(Ub),immunoglobulin-binding b1 domain of streptococcal protein G(GB1),and second mitochondria-derived activator of caspases(Smac).The NOAI protein conjugate connected by a thioester bond is resistant to reducing reagent and offers highfidelity DEER distance measurements in cell lysates.
文摘This study summarizes the examination data of registration labels for ordinary cosmetics in Beijing from May 2021 to April 2024.It analyzes and categorizes the issues identified during label evaluations,explores the underlying causes,and proposes regulatory countermeasures and recommendations for registrants,regulatory authorities,and social organizations.The objective is to offer practical insights and regulatory guidance to support the enhancement of cosmetic registration and regulatory standards.
基金funded by the Ongoing Research Funding program(ORF-2025-867),King Saud University,Riyadh,Saudi Arabia.
文摘Digital twin technology is revolutionizing personalized healthcare by creating dynamic virtual replicas of individual patients.This paper presents a novel multi-modal architecture leveraging digital twins to enhance precision in predictive diagnostics and treatment planning of phoneme labeling.By integrating real-time images,electronic health records,and genomic information,the system enables personalized simulations for disease progression modeling,treatment response prediction,and preventive care strategies.In dysarthric speech,which is characterized by articulation imprecision,temporal misalignments,and phoneme distortions,existing models struggle to capture these irregularities.Traditional approaches,often relying solely on audio features,fail to address the full complexity of phoneme variations,leading to increased phoneme error rates(PER)and word error rates(WER).To overcome these challenges,we propose a novel multi-modal architecture that integrates both audio and articulatory data through a combination of Temporal Convolutional Networks(TCNs),Graph Convolutional Networks(GCNs),Transformer Encoders,and a cross-modal attention mechanism.The audio branch of the model utilizes TCNs and Transformer Encoders to capture both short-and long-term dependencies in the audio signal,while the articulatory branch leverages GCNs to model spatial relationships between articulators,such as the lips,jaw,and tongue,allowing the model to detect subtle articulatory imprecisions.A cross-modal attention mechanism fuses the encoded audio and articulatory features,enabling dynamic adjustment of the model’s focus depending on input quality,which significantly improves phoneme labeling accuracy.The proposed model consistently outperforms existing methods,achieving lower Phoneme Error Rates(PER),Word Error Rates(WER),and Articulatory Feature Misclassification Rates(AFMR).Specifically,across all datasets,the model achieves an average PER of 13.43%,an average WER of 21.67%,and an average AFMR of 12.73%.By capturing both the acoustic and articulatory intricacies of speech,this comprehensive approach not only improves phoneme labeling precision but also marks substantial progress in speech recognition technology for individuals with dysarthria.
文摘Since the 1970s,a series of international and national sources have supported the principle of accessibility,which slowly has become a statuary norm and a legislative obligation.Each country has implemented accessibility through a singular policy.But in addition to the accessibility of a place or an activity,to inform about what is accessible is very important as well,and has not really taken off.Indeed,for disabled people,the difficulty lies not only with access to places and the use of resources,but also with the visibility of these resources.This means that information concerning accessibility has to be disclosed and provided effectively to disabled people,those involved with them and the relevant institutions.In different countries all over the world,many labels and pictograms have been created for this purpose and give information relating to accessibility.Using a socio-historical approach,we will present and analyze the different types of icons,symbols,pictograms and labels that have been put in place around the world and in France:what are they used for and for whom are they made?We will show that they are pointers which firstly reflect the diversity and range within the target group concerned by accessibility,and secondly the evolution of accessibility as a dynamic and ecological principle.
文摘This paper is concerned with design-ing symbol labeling for a low-density parity-check(LDPC)-coded delayed bit-interleaved coded modu-lation(DBICM)scheme in a two-way relay channel(TWRC).We first present some properties of symbol labeling within a phase shift keying(PSK)modula-tion.These properties reduce the candidate labeling search space.Based on this search space,we take DBICM capacity as the cost function and propose a general method for optimizing symbol labeling by em-ploying the differential evolution algorithm.Numeri-cal results show that our labeling obtains a signal-to-noise ratio(SNR)gain up to 0.45 dB with respect to Gray labeling.
基金supported by the National Natural Science Foundation of China(Nos.22264013,21961010)Hainan Province Science and Technology Special Fund(Nos.ZDYF2021SHFZ219,ZDYF2022SHFZ037)+4 种基金Special Funds of S&T Cooperation and Exchange Projects of Shanxi Province(No.202204041101040)Natural Science Research Talent Project of Hainan Medical University(No.JBGS202101)Postgraduate Innovative Research Project of Hainan(No.Qhys2021-384)Hainan Province Clinical Medical Center(2021)Project for Functional Materials and Molecular Imaging Science Innovation Group of Hainan Medical University.
文摘Acute lung injury(ALI)is a serious clinical condition with a high mortality rate.Oxidative stress and inflammatory responses play pivotal roles in the pathogenesis of ALI.ONOO^(−)is a key mediator that exacerbates oxidative damage and microvascular permeability in ALI.Accurate detection of ONOO^(−)would facilitate early diagnosis and intervention in ALI.Near-infrared fluorescence(NIRF)probes offer new solutions due to their sensitivity,depth of tissue penetration,and imaging capabilities.However,the developed ONOO^(−)fluorescent probes face problems such as interference from other reactive oxygen species and easy intracellular diffusion.To address these issues,we introduced an innovative self-immobilizing NIRF probe,DCI2F-OTf,which was capable of monitoring ONOO^(−)in vitro and in vivo.Importantly,leveraging the high reactivity of the methylene quinone(QM)intermediate,DCI2F-OTf was able to covalently label proteins in the presence of ONOO^(−),enabling in situ imaging.In mice models of ALI,DCI2F-OTf enabled real-time imaging of ONOO^(−)levels and found that ONOO^(−)was tightly correlated with the progression of ALI.Our findings demonstrated that DCI2F-OTf was a promising chemical tool for the detection of ONOO^(−),which could help to gain insight into the pathogenesis of ALI and monitor treatment efficacy.
基金granted by Key Project of Beijing Municipal Social Science Foundation(No.15ZHA004)Key Project of Beijing Municipal Social Science Foundation and Beijing Municipal Education Commission Social Science Program(No.SZ20231123202).
文摘Many existing immune detection algorithms rely on a large volume of labeled self-training samples,which are often difficult to obtain in practical scenarios,thus limiting the training of detection models.Furthermore,noise inherent in the samples can substantially degrade the detection accuracy of these algorithms.To overcome these challenges,we propose an immune generation algorithm that leverages clustering and a rebound mechanism for label propagation(LP-CRI).The dataset is randomly partitioned into multiple subsets,each of which undergoes clustering followed by label propagation and evaluation.The rebound mechanism assesses the model’s performance after propagation and determines whether to revert to its previous state,initiating a subsequent round of propagation to ensure stable and effective training.Experimental results demonstrate that the proposed method is both computationally efficient and easy to train,significantly enhancing detector performance and outperforming traditional immune detection algorithms.
基金supported in part by the National Key R&D Program of China under Grant 2022YFB4300601in part by the State Key Laboratory of Advanced Rail Autonomous Operation under Grant RAO2023ZZ003.
文摘Intelligent fault diagnosis technology plays an indispensable role in ensuring the safety,stability,and efficiency of railway operations.However,existing studies have the following limitations.1)They are typical black-box models that lacks interpretability as well as they fuse features by simply stacking them,overlooking the discrepancies in the importance of different features,which reduces the credibility and diagnosis accuracy of the models.2)They ignore the effects of potentially mistaken labels in the training datasets disrupting the ability of the models to learn the true data distribution,which degrades the generalization performance of intelligent diagnosis models,especially when the training samples are limited.To address the above items,an interpretable few-shot framework for fault diagnosis with noisy labels is proposed for train transmission systems.In the proposed framework,a feature extractor is constructed by stacked frequency band focus modules,which can capture signal features in different frequency bands and further adaptively concentrate on the features corresponding to the potential fault characteristic frequency.Then,according to prototypical network,a novel metric-based classifier is developed that is tolerant to mislabeled support samples in the case of limited samples.Besides,a new loss function is designed to decrease the impact of label mistakes in query datasets.Finally,fault simulation experiments of subway train transmission systems are designed and conducted,and the effectiveness as well as superiority of the proposed method are proved by ablation experiments and comparison with the existing methods.
基金supported by the Special Fund for Scientific and Technological Innovation Strategy of Guangdong Province(2022A0505030025)the Science and Technology Fund,FDCT,Macao SAR(0064/2021/A2)
文摘This paper addresses the verification of strong currentstate opacity with respect to real-time observations generated from a discrete-event system that is modeled with time labeled Petri nets. The standard current-state opacity cannot completely characterize higher-level security. To ensure the higher-level security requirements of a time-dependent system, we propose a strong version of opacity known as strong current-state opacity. For any path(state-event sequence with time information)π derived from a real-time observation that ends at a secret state, the strong current-state opacity of the real-time observation signifies that there is a non-secret path with the same real-time observation as π. We propose general and non-secret state class graphs, which characterize the general and non-secret states of time-dependent systems, respectively. To capture the observable behavior of non-secret states, a non-secret observer is proposed.Finally, we develop a structure called a real-time concurrent verifier to verify the strong current-state opacity of time labeled Petri nets. This approach is efficient since the real-time concurrent verifier can be constructed by solving a certain number of linear programming problems.
文摘To extract and display the significant information of combat systems,this paper introduces the methodology of functional cartography into combat networks and proposes an integrated framework named“functional cartography of heterogeneous combat networks based on the operational chain”(FCBOC).In this framework,a functional module detection algorithm named operational chain-based label propagation algorithm(OCLPA),which considers the cooperation and interactions among combat entities and can thus naturally tackle network heterogeneity,is proposed to identify the functional modules of the network.Then,the nodes and their modules are classified into different roles according to their properties.A case study shows that FCBOC can provide a simplified description of disorderly information of combat networks and enable us to identify their functional and structural network characteristics.The results provide useful information to help commanders make precise and accurate decisions regarding the protection,disintegration or optimization of combat networks.Three algorithms are also compared with OCLPA to show that FCBOC can most effectively find functional modules with practical meaning.
基金Hubei Natural Science Foundation of China,Grant/Award Number:2024AFB593。
文摘Background:Rabies virus(RABV)-derived neuronal tracing tools are extensively applied in retrograde tracing due to their strict retrograde transsynaptic transfer property and low neurotoxicity.However,the RABV infection and expression of fluorescence products would be gradually cleared while the infected neurons still survive,a phenomenon known as non-cytolytic immune clearance(NCLIC).This phenomenon introduced the risk of fluorescence loss and led to the omission of a subset of neurons that should be labeled,thereby interfering in the analysis of tracing results.Methods:To compensate for the fluorescence loss problem,in this study,we developed a novel marker footprints(MF)mouse,involving a Cre recombinase-dependent red fluorescent reporter system and systemic expression of glycoprotein(G)and ASLV-A receptor(TVA).Using this mouse model combined with the well-developed RABV-EnvA-ΔG-GFP-Cre viral tool,we developed a novel green-to-red spectral labeling strategy.Results:Neurons in the MF mouse could be co-labeled with green fluorescence from the very quick expression of the viral tool and with red fluorescence from the relatively slow expression of the neuron itself,so neurons undergoing NCLIC with green fluorescence loss could be relabeled red.Furthermore,newly infected neurons could be labeled green and other neurons could be labeled yellow due to the temporal expression difference between the two fluorescent proteins.Conclusions:This is the first polysynaptic retrograde tracing labeling strategy that could label neurons using spectral fluorescence colors with only one injection of the viral tool,enabling its application in recognizing the labeling sequence of neurons in brain regions and enhancing the spatiotemporal resolution of neuronal tracing.
基金supported by National Key Research and Development Program of China(Grant No.:2023YFF0716000)National Natural Science Foundation of China(Grant No.:82071965)+1 种基金Major plan of Jointly Constructed Project by the Science and Technology Department of the State Administration of Traditional Chinese Medicine and the Zhejiang Provincial Administration of Traditional Chinese Medicine,China(Grant No.:GZY-ZJ-KJ-24025)Zhejiang Provincial Natural Science Foundation of China(Grant No.:LQ23H180005).
文摘Nanoclays have large specific surface area,good adsorption properties,and biocompatibility that have great potential for drug delivery applications[1].Evaluating the in vivo metabolic pathways of nanoclays can help to understand their pharmacodynamic sites and the toxicological effects caused by their in vivo retention time[2].
基金supported by the National Natural Science Foundation of China under Grant Nos.62461037,62076117 and 62166026the Jiangxi Provincial Natural Science Foundation under Grant Nos.20224BAB212011,20232BAB202051,20232BAB212008 and 20242BAB25078the Jiangxi Provincial Key Laboratory of Virtual Reality under Grant No.2024SSY03151.
文摘The unsupervised vehicle re-identification task aims at identifying specific vehicles in surveillance videos without utilizing annotation information.Due to the higher similarity in appearance between vehicles compared to pedestrians,pseudo-labels generated through clustering are ineffective in mitigating the impact of noise,and the feature distance between inter-class and intra-class has not been adequately improved.To address the aforementioned issues,we design a dual contrastive learning method based on knowledge distillation.During each iteration,we utilize a teacher model to randomly partition the entire dataset into two sub-domains based on clustering pseudo-label categories.By conducting contrastive learning between the two student models,we extract more discernible vehicle identity cues to improve the problem of imbalanced data distribution.Subsequently,we propose a context-aware pseudo label refinement strategy that leverages contextual features by progressively associating granularity information from different bottleneck blocks.To produce more trustworthy pseudo-labels and lessen noise interference during the clustering process,the context-aware scores are obtained by calculating the similarity between global features and contextual ones,which are subsequently added to the pseudo-label encoding process.The proposed method has achieved excellent performance in overcoming label noise and optimizing data distribution through extensive experimental results on publicly available datasets.
基金funded by the National Natural Science Foundation of China (Nos. 62235007 and 22204070)the National Key Research and Development Program (No. 2020YFA0909000)+2 种基金Guangdong Provincial Key Laboratory of Advanced Biomaterials (No. 2022B1212010003)Shenzhen Science and Technology Innovation Project (Nos. KQTD20170810111314625, SGDX20211123114002003 and JCYJ20210324115807021)Shenzhen Bay Laboratory (No. SZBL2021080601002).
文摘Self-labeling protein (SLP) tags, such as HaloTag, have gained considerable interest as advanced tools for live cell labeling. However, the chloroalkane-based substrates that can be directly used for protein labeling are limited. Here, we report two bioorthogonal small molecule linkers, chloroalkane-tetrazine (CA-Tz) and chloroalkane-azide (CA-N3), which can penetrate cell membranes and facilitate click chemistry-based labeling in live cells. We compare their labeling capability using two clickable silicon rhodamine dyes (SiR-PEG_(3)-TCO and SiR-PEG_(4)-DBCO). Confocal imaging results demonstrate that using CA-Tz and SiR-PEG_(3)-TCO dye exhibits superior intracellular labeling with low nonspecific signals. We subsequently compared the photostability of SiR dyes with that of green fluorescent proteins (mEmerald). Total internal reflection fluorescence (TIRF) imaging indicates that SiR dyes exhibit superior photostability under identical excitation conditions, making them suitable for long-term cell imaging. Furthermore, SiR dyes labeling also shows high structure retention for the fourth-order super-resolution optical fluctuation imaging (SOFI) compared to fluorescent proteins. This study presents clickable HaloTag linkers as effective tools for live cell labeling and imaging, highlighting the high-quality labeling of chloroalkane linkers and clickable dyes for live cell imaging.
