AIM： To evaluate the reproducibility of a modified 13^C breath test-based measurement of solid phase gastric emptying （GE） within the frames of a simple-repeated measure study protocol. METHODS： Twelve healthy sub...AIM： To evaluate the reproducibility of a modified 13^C breath test-based measurement of solid phase gastric emptying （GE） within the frames of a simple-repeated measure study protocol. METHODS： Twelve healthy subjects （6 females and 6 males, mean age 24.9＋0.7 years） were recruited to undergo three identical GE examinations. In six subjects the first two examinations were performed 2 d apart, and the third session was carried out at a median interval of 19.5 d （range 18 - 20 d） from the second one. In another six subjects the first two measurements were taken 20 d apart （median, range： 17-23 d）, whereas the third session took place 2 d after the second one. Probes of expiratory air collected before and during six hours after intake of a solid meal （378 kcal） labelled with 75 μL （68 mg） 13^C-octanoic acid, were measured for 13^CO2 enrichment with the nondispersive isotopeselective infrared spectrometry NDIRS apparatus. RESULTS： Taking coefficients of variation for paired examinations into account, the short-term reproducibility of the GE measurement was slightly but not significantly better than the medium-term one： 7.7% and 11.2% for the lag phase （T-Lag）, 7.3% and 10.9% for the gastric half emptying time （T1/2）. The least differences in GE parameters detectable at P= 0.05 level in the 12 paired examinations were 9.6 and 15.6 min for T-Lag, 11.6 and 19.7 min for T1/2 by a two-day or two to three-week time gap, respectively CONCLUSION： The low-cost modification of the breath test involving a lower dose of 13^C-octanoic acid and NDIRS, renders good short- and medium-term reproducibility, as well as sensitivity of the measurement of gastric emptying of solids.展开更多
基金Supported by a research grant(3 P05D 054 24)from the Ministry of Scientific Research and Information Technology(formerly:State Committee For Scientific Research)of the Republic of Poland-contract #0617/P05/2003/24
文摘AIM： To evaluate the reproducibility of a modified 13^C breath test-based measurement of solid phase gastric emptying （GE） within the frames of a simple-repeated measure study protocol. METHODS： Twelve healthy subjects （6 females and 6 males, mean age 24.9＋0.7 years） were recruited to undergo three identical GE examinations. In six subjects the first two examinations were performed 2 d apart, and the third session was carried out at a median interval of 19.5 d （range 18 - 20 d） from the second one. In another six subjects the first two measurements were taken 20 d apart （median, range： 17-23 d）, whereas the third session took place 2 d after the second one. Probes of expiratory air collected before and during six hours after intake of a solid meal （378 kcal） labelled with 75 μL （68 mg） 13^C-octanoic acid, were measured for 13^CO2 enrichment with the nondispersive isotopeselective infrared spectrometry NDIRS apparatus. RESULTS： Taking coefficients of variation for paired examinations into account, the short-term reproducibility of the GE measurement was slightly but not significantly better than the medium-term one： 7.7% and 11.2% for the lag phase （T-Lag）, 7.3% and 10.9% for the gastric half emptying time （T1/2）. The least differences in GE parameters detectable at P= 0.05 level in the 12 paired examinations were 9.6 and 15.6 min for T-Lag, 11.6 and 19.7 min for T1/2 by a two-day or two to three-week time gap, respectively CONCLUSION： The low-cost modification of the breath test involving a lower dose of 13^C-octanoic acid and NDIRS, renders good short- and medium-term reproducibility, as well as sensitivity of the measurement of gastric emptying of solids.
