MicroRNAs(miRNAs) are a class of small noncoding RNAs that post-transcriptionally regulate the expression of many target genes via mRNA degradation or translation inhibition. Many studies have shown that miRNAs are in...MicroRNAs(miRNAs) are a class of small noncoding RNAs that post-transcriptionally regulate the expression of many target genes via mRNA degradation or translation inhibition. Many studies have shown that miRNAs are involved in the modulation of gene expression and replication of hepatitis B virus(HBV) and hepatitis C virus(HCV) and play a pivotal role in host-virus interactions. Increasing evidence also demonstrates that viral infection leads to alteration of the miRNA expression profile in hepatic tissues or circulation. The deregulated miRNAs participate in hepatocellular carcinoma(HCC)initiation and progression by functioning as oncogenes or tumor suppressor genes by targeting various genes involved in cancer-related signaling pathways. The distinct expression pattern of miRNAs may be a useful marker for the diagnosis and prognosis of virus-related diseases considering the limitation of currently used biomarkers. Moreover, the role of deregulated miRNA in host-virus interactions and HCC development suggested that miRNAs may serve as therapeutic targets or astools. In this review, we summarize the recent findings about the deregulation and the role of miRNAs during HBV/HCV infection and HCC development, and we discuss the possible mechanism of action of miRNAs in the pathogenesis of virus-related diseases. Furthermore, we discuss the potential of using miRNAs as markers for diagnosis and prognosis as well as therapeutic targets and drugs.展开更多
Recent findings demonstrate the common genetic basis for many immune-mediated diseases, and consequently, the partially shared pathogenesis. We collected these findings and reviewed the extension of these overlaps to ...Recent findings demonstrate the common genetic basis for many immune-mediated diseases, and consequently, the partially shared pathogenesis. We collected these findings and reviewed the extension of these overlaps to other disease characteristics. Two autoimmune diseases were selected that also share the specific target organ, the bowel. The etiology and immunopathogenesis of both conditions characterized by chronic intestinal inflammation, inflammatory bowel disease (IBD) and celiac disease (CeD), are not completely understood. Both are complex diseases with genetics and environment contributing to dysregulation of innate and adaptive immune responses, leading to chronic inflammation and disease. CeD constitutes a particular disease because the main environmental and genetic triggers are largely known. IBD comprises two main clinical forms, Crohn’s disease and ulcerative colitis, which most likely involve a complex interplay between some components of the commensal microbiota and other environmental factors in their origin. These multifactorial diseases encompass a broad spectrum of clinical phenotypes and ages of onset, although the clinical presentation often differs depending on childhood or adult onset, with greater heterogeneity commonly observed in adults.展开更多
This paper aims to discus some practical ways to develop the learners’autonomous English learning abilities.After analyzed the motivation problems,the author strengthened learners’motivation through classroom teachi...This paper aims to discus some practical ways to develop the learners’autonomous English learning abilities.After analyzed the motivation problems,the author strengthened learners’motivation through classroom teaching successfully by building good social relations,promoting negotiation,promoting interaction in the classroom and setting up adequate evaluation systems,etc.The author finds that using the content of regular college classroom teaching as a meaningful context for the development of responsibility can enhance the learning effect.The teacher’s guiding role in autonomous learning should never be ignored.Developing autonomous English learning in vocational colleges in China is our long-term pedagogical goal.展开更多
The ribosome,a 2.6 megadalton biomolecule measuring approximately 20 nm in diameter,coordinates numerous ligands,factors,and regulators to translate proteins with high fidelity and speed.Understanding its complex func...The ribosome,a 2.6 megadalton biomolecule measuring approximately 20 nm in diameter,coordinates numerous ligands,factors,and regulators to translate proteins with high fidelity and speed.Understanding its complex functions necessitates multiperspective observations.We developed a dualFRET single-molecule Förste Resonance Energy Transfer method(dual-smFRET),allowing simultaneous observation and correlation of tRNA dynamics and Elongation Factor G(EF-G)conformations in the same complex,in a 10 s time window.By synchronizing laser shutters and motorized filter sets,two FRET signals are captured in consecutive 5 s intervals with a time gap of 50-100 ms.We observed distinct fluorescent emissions from single-,double-,and quadruple-labeled ribosome complexes.Through comprehensive spectrum analysis and correction,we distinguish and correlate conformational changes in two parts of the ribosome,offering additional perspectives on its coordination and timing during translocation.Our setup’s versatility,accommodating up to six FRET pairs,suggests broader applications in studying large biomolecules and various biological systems.展开更多
Paper mulberry(Broussonetia papyrifera)is a well-known woody tree historically used for Cai Lun papermaking,one of the four great inventions of ancient China.More recently,Paper mulberry has also been used as forage t...Paper mulberry(Broussonetia papyrifera)is a well-known woody tree historically used for Cai Lun papermaking,one of the four great inventions of ancient China.More recently,Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.In this study,we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches,including Illumina and PacBio sequencing platform as well as Hi-C,optical,and genetic maps.The assembled Paper mulberry genome consists of 386.83 Mb,which is close to the estimated size,and 99.25%(383.93 Mb)of the assembly was assigned to 13 pseudochromosomes.Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families,respectively,accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.Moreover,the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine,forage,papermaking,and barkcloth making.We also identified the rootassociated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.Collectively,these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making,and as forage.These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide.展开更多
基金Supported by National Natural Science Foundation of China,No.91029714,No.31071191,No.31270818 and No.31101000Natural Science Foundation of Tianjin,No.09JCZDJC17500 and No.12JCZDJC25100
文摘MicroRNAs(miRNAs) are a class of small noncoding RNAs that post-transcriptionally regulate the expression of many target genes via mRNA degradation or translation inhibition. Many studies have shown that miRNAs are involved in the modulation of gene expression and replication of hepatitis B virus(HBV) and hepatitis C virus(HCV) and play a pivotal role in host-virus interactions. Increasing evidence also demonstrates that viral infection leads to alteration of the miRNA expression profile in hepatic tissues or circulation. The deregulated miRNAs participate in hepatocellular carcinoma(HCC)initiation and progression by functioning as oncogenes or tumor suppressor genes by targeting various genes involved in cancer-related signaling pathways. The distinct expression pattern of miRNAs may be a useful marker for the diagnosis and prognosis of virus-related diseases considering the limitation of currently used biomarkers. Moreover, the role of deregulated miRNA in host-virus interactions and HCC development suggested that miRNAs may serve as therapeutic targets or astools. In this review, we summarize the recent findings about the deregulation and the role of miRNAs during HBV/HCV infection and HCC development, and we discuss the possible mechanism of action of miRNAs in the pathogenesis of virus-related diseases. Furthermore, we discuss the potential of using miRNAs as markers for diagnosis and prognosis as well as therapeutic targets and drugs.
基金Supported by Grants from"Fondo de Investigaciones Sanitarias",PI11/00614"Fundación Eugenio Rodríguez Pascual"
文摘Recent findings demonstrate the common genetic basis for many immune-mediated diseases, and consequently, the partially shared pathogenesis. We collected these findings and reviewed the extension of these overlaps to other disease characteristics. Two autoimmune diseases were selected that also share the specific target organ, the bowel. The etiology and immunopathogenesis of both conditions characterized by chronic intestinal inflammation, inflammatory bowel disease (IBD) and celiac disease (CeD), are not completely understood. Both are complex diseases with genetics and environment contributing to dysregulation of innate and adaptive immune responses, leading to chronic inflammation and disease. CeD constitutes a particular disease because the main environmental and genetic triggers are largely known. IBD comprises two main clinical forms, Crohn’s disease and ulcerative colitis, which most likely involve a complex interplay between some components of the commensal microbiota and other environmental factors in their origin. These multifactorial diseases encompass a broad spectrum of clinical phenotypes and ages of onset, although the clinical presentation often differs depending on childhood or adult onset, with greater heterogeneity commonly observed in adults.
文摘This paper aims to discus some practical ways to develop the learners’autonomous English learning abilities.After analyzed the motivation problems,the author strengthened learners’motivation through classroom teaching successfully by building good social relations,promoting negotiation,promoting interaction in the classroom and setting up adequate evaluation systems,etc.The author finds that using the content of regular college classroom teaching as a meaningful context for the development of responsibility can enhance the learning effect.The teacher’s guiding role in autonomous learning should never be ignored.Developing autonomous English learning in vocational colleges in China is our long-term pedagogical goal.
基金supported by NIGMS grant:R01GM111452,NSF:2130427the Welch Foundation:E-1721 to Y.Wang。
文摘The ribosome,a 2.6 megadalton biomolecule measuring approximately 20 nm in diameter,coordinates numerous ligands,factors,and regulators to translate proteins with high fidelity and speed.Understanding its complex functions necessitates multiperspective observations.We developed a dualFRET single-molecule Förste Resonance Energy Transfer method(dual-smFRET),allowing simultaneous observation and correlation of tRNA dynamics and Elongation Factor G(EF-G)conformations in the same complex,in a 10 s time window.By synchronizing laser shutters and motorized filter sets,two FRET signals are captured in consecutive 5 s intervals with a time gap of 50-100 ms.We observed distinct fluorescent emissions from single-,double-,and quadruple-labeled ribosome complexes.Through comprehensive spectrum analysis and correction,we distinguish and correlate conformational changes in two parts of the ribosome,offering additional perspectives on its coordination and timing during translocation.Our setup’s versatility,accommodating up to six FRET pairs,suggests broader applications in studying large biomolecules and various biological systems.
基金the National Natural Science Foundation of China(31770360,31870247)the Poverty Relief Project of the Chinese Academy of Sciences(KFJ-FP-24)the Huimin Technology Demonstration Project of the Natio nal Modem Agricultural Science and Technology Achievements City(Z151100001015008).
文摘Paper mulberry(Broussonetia papyrifera)is a well-known woody tree historically used for Cai Lun papermaking,one of the four great inventions of ancient China.More recently,Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.In this study,we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches,including Illumina and PacBio sequencing platform as well as Hi-C,optical,and genetic maps.The assembled Paper mulberry genome consists of 386.83 Mb,which is close to the estimated size,and 99.25%(383.93 Mb)of the assembly was assigned to 13 pseudochromosomes.Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families,respectively,accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.Moreover,the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine,forage,papermaking,and barkcloth making.We also identified the rootassociated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.Collectively,these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making,and as forage.These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide.
