Background： The aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization. Wheat straw and wood chips were treated with Ceriporiopsis subvermispora and Lentinul...Background： The aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization. Wheat straw and wood chips were treated with Ceriporiopsis subvermispora and Lentinula edodes with various amounts of colonized millet grains（0.5, 1.5 or 3.0 % per g of wet weight of substrate） added to the substrates. Also, wheat straw and wood chips were chopped to either 0.5 or 2 cm.Effectiveness of the fungal treatment after 0, 2, 4, 6, or 8 wk of incubation was determined by changes in chemical composition, in vitro gas production（IVGP） as a measure for rumen degradability, and ergosterol content as a measure of fungal biomass.Results： Incomplete colonization was observed for C. subvermispora treated wheat straw and L. edodes treated wood chips. The different particle sizes and amounts of inoculum tested, had no significant effects on the chemical composition and the IVGP of C. subvermispora treated wood chips. Particle size did influence L.edodes treatment of wheat straw. The L. edodes treatment of 2 cm wheat straw resulted in a more selective delignification and a higher IVGP than the smaller particles. Addition of 1.5 % or 3 % L. edodes inoculum to wheat straw resulted in more selective delignification and a higher IVGP than addition of 0.5 % inoculum.Conclusion： Particle size and amount of inoculum did not have an effect on C. subvermispora treatment of wood chips. At least 1.5 % L. edodes colonized millet grains should be added to 2 cm wheat straw to result in an increased IVGP and acid detergent lignin（ADL） degradation.展开更多
The effects of inoculum forms (single-spore, multi-spores, or colonized root pieces) and host plants (Nicotiana tabacum L., Sorghum sudanense (Piper) Stapf, and Trifolium repens L.) on the development and inoculum pot...The effects of inoculum forms (single-spore, multi-spores, or colonized root pieces) and host plants (Nicotiana tabacum L., Sorghum sudanense (Piper) Stapf, and Trifolium repens L.) on the development and inoculum potential (IP) of the arbuscular mycorrhizal fungi (AMF) :Glo-mus macrocarpum Tul & Tul, donuis mosseae (Nicol & Gerd.) Gerdemann & Trappe, Glomus ver-siforme (Karsten) Berch, and Sclerocystis sinu/osa Gerdemann & Bakhi cultured in pots were investigated. The lag phase of treatment with 50 spores or 0.5 g (fresh weight) of colonized root pieces was 4 weeks, much shorter than that of the treatment with 1 spore (8 weeks); the value of IP (VIP) and percentage of root colonization (PRC) of the former were greater than those of the latter. Only on the early stages of colonization was there difference between the 50 spores and the 0.5 g (fresh weight) of colonized root piece inoculation treatments. The EP per plant inoculated with 0.5 g (fresh weight) of colonized root pieces of AMF was greater than that of the other two treatments except G. versiforme on Nicotiana tabacum, while the PRC of the plants inoculated with 50 spores and 0.5 g (fresh weight) of colonized root pieces of AMF was higher than that of the 1 spore inoculation after 10 weeks. Trie VIP of AMF on Trifolium repens was significantly higher than that on the other two hosts. The VIP of G. mosseae, G. versiforme, and S. sinuosa was respectively greater than that of G. macrocarpum. This suggested that different species of AMF produced different VIP of the inoculum . Nicotiana tabacum was much better than the other host plants which used to be inoculated with single spore, and to produce inocula of AMF.展开更多
The research designed four application quantities of 0, 2 250, 4 500 and 6 750 g/hm2 based on Liangyou No. 959 to explore the effects of microbial inocu- lure fertilizer on growth and yield of super hybrid rice. The r...The research designed four application quantities of 0, 2 250, 4 500 and 6 750 g/hm2 based on Liangyou No. 959 to explore the effects of microbial inocu- lure fertilizer on growth and yield of super hybrid rice. The results indicate that the treatment with traditional fertilizers and 4 500 g/hm2 HYT microbial inoculum fertilizer performed the best, where average number of tiller was 2.4 more than that of the control; the number of effective ear was 11.05% higher; yield increased by 10.3%. These have demonstrated that microbial inoculum fertilizer is of great value in pro- motion on super hybrid rice production.展开更多
Invasive pests and pathogens cause immense damage globally,costing an estimated US$248 billion to the agricultural industry alone.Vehicles,such as farming and timber harvesting machinery and transportation trucks,can ...Invasive pests and pathogens cause immense damage globally,costing an estimated US$248 billion to the agricultural industry alone.Vehicles,such as farming and timber harvesting machinery and transportation trucks,can facilitate the rapid spread of biological invaders over distances far greater and more quickly than their natural dispersal ability.Understanding how frequent trips by these vehicles increase the spread of invasive agricultural and forestry pests can help inform effective biosecurity procedures before,during,or after an incursion.We used a case study of timber transport trucks in Aotearoa New Zealand to examine whether and how vehicles facilitate the spread of soil-borne pathogens between commercial forest plantations.Our results show that long-distance dispersal associated with truck movement facilitated the introduction of oomycete-like pathogens in 97% of forest sites within only one year,with pathogen loads within infected sites predicted at 84%of the sites’carrying capacity.Implementing preventative management strategies to reduce the transportation of infected soil by logging trucks,however,can reduce the spread by up to 50% after one year and reduce the pathogen load within infested sites by more than three times.Mitigating other human-assisted dispersal pathways can also help reduce spread.Reducing movement of forest visitors not involved in forestry activities,for instance,by closing forest sites to the public,can help to further reduce spread in addition to management related to harvesting activities.These results highlight the benefits of preventative management strategies in reducing the spread rate of novel soil pathogens through a high-intensity commercial forestry network but show that pest spread is still likely even with significant investment.展开更多
Through a pot experiment, effects of various microbial inoculums on soil microbial diversity and enzyme activity in a typical reclaimed mining area in Shanxi Province were discussed based on quantitative analysis of P...Through a pot experiment, effects of various microbial inoculums on soil microbial diversity and enzyme activity in a typical reclaimed mining area in Shanxi Province were discussed based on quantitative analysis of PLFA, soil urease, phosphatase and sucrase activity. The results showed that the application of microbial inoculums increased microorganism quantity in rhizosphere of rape by 2.3% -66.4%, and quantities of bac- teria, gram-positive bacteria, gram-negative bacteria, fungi and actinomycetes in the treatments with microbial inoculums were significantly higher than the contrast （P 〈0.05）, while there was no obvious change in protist quantity. In comparison with the contrast, the application of various mi- crobial inoculums also improved soil urease, sucrase and phosphatase activity by 4.2% - 61.4%, 18.0% - 32.5% and 64.2% - 199.0% respec- tively. It indicated that the application of microbial inoculums can improve soil microbial diversity and enzyme activity, so it is an effective way to sl^eed UP ecoloQical restoration of soil.展开更多
Lactate is a key intermediate during anaerobic digestion of carbohydrates;however, it fails to receive significant consideration in biogas plants. We examined the influence of lactic acid on biogas production. Two com...Lactate is a key intermediate during anaerobic digestion of carbohydrates;however, it fails to receive significant consideration in biogas plants. We examined the influence of lactic acid on biogas production. Two commonly used feeds, fresh maize and maize silage, were selected as substrates due to their difference in lactic acid contents. Additionally, inocula from an agriculture-based biogas plant, a waste water treatment plant and a standardised laboratory reactor were selected to investigate the impact of starter culture on the process. Experiments demonstrated increased total biogas yield of up to 45% in the lactate-rich maize silage over the lactate-devoid fresh maize, but only in cases where the starting inocula had been previously exposed to lactic acid. Our findings suggest lactic acid is a significant intermediate in biogas production and merits consideration. Additionally, the ability of the starter inoculum to utilize lactic acid is an important factor in process optimization and enhanced biogas production.展开更多
[Objectives]This study was conducted to understand the effects of microbial inocula on the biological yield and nutritional components of corn.[Methods]Silage corn varieties suitable for planting in Hebei Province wer...[Objectives]This study was conducted to understand the effects of microbial inocula on the biological yield and nutritional components of corn.[Methods]Silage corn varieties suitable for planting in Hebei Province were selected,and set with an experimental group and a control check group each.Meanwhile,at the late stage of milk ripening of silage corn growth cycle,the nutritional components and indexes of silage corn were determined and analyzed by a near-infrared analyzer and the NIRS technique.Meanwhile,the biological yield of silage corn was determined at the maturation stage of its growth period,aiming at comprehensively evaluating the regulation effects of the microbial inoculum tested on silage corn from the aspects of basic nutritional components,mineral element contents,energy and related indexes of corn,combined with agronomic characters and biological yield.[Results]The microbial inoculum improved the biological yield and nutritional indexes of silage corn,and had a positive regulation effect on the growth of silage corn.[Conclusions]The results of this study provide a theoretical basis for popularizing the planting methods and cultivation methods of silage corn with high yield and high quality.展开更多
Poor land use management and practice inhibit the growth and establishment of tree seedlings in dryland areas.We assessed arbuscular mycorrhizal fungi(AM)status of Faidherbia albida(Del.)A.Chev.trees grown on differen...Poor land use management and practice inhibit the growth and establishment of tree seedlings in dryland areas.We assessed arbuscular mycorrhizal fungi(AM)status of Faidherbia albida(Del.)A.Chev.trees grown on different land uses.We quantified the growth and nutrient uptake of F.albida seedlings inoculated with AM from different sources.These efforts were based on soil and fine root samples from the rhizosphere soils of F.albida trees.AM root colonization was determined using the gridline intersect method.Spores were extracted by the wet sieving and decanting method and identified to genus level.The seedling experiment had a completely randomized onefactorial design with four treatments and five replications.Faidherbida albida seedlings were grown in a greenhouse.All in situ F.albida trees were colonized by AM fungi.AM root colonization of F.albida trees was significantly higher(P<0.0086)in area exclosures than on lands used for grazing or cultivation.Spore abundance was significantly higher(P<0.0014)in area exclosures followed by cultivated land and grazing land.Glomus was the dominant genus in all land-uses.AM-inoculated F.albida seedlings grew better(P<0.05)than non-inoculated controls.Seedlings inoculated with AM from area exclosure had significantly(P<0.05)higher growth and nutrient uptake than those inoculated with AM from grazing and cultivated land.This emphasizes the importance of the native soil AM potential for better establishment of seedlings to achieve optimum plant growth improvement and assist in rehabilitation of degraded arid lands.展开更多
An experiment was conducted in pots under natural condition with two factor factorial completely randomized design (CRD) (CRD) to investigate the integrated effect of rhizobia inoculums and inorganic fertilizers on gr...An experiment was conducted in pots under natural condition with two factor factorial completely randomized design (CRD) (CRD) to investigate the integrated effect of rhizobia inoculums and inorganic fertilizers on growth and yield of wheat crop at The University of Agriculture Peshawar, during 2012-2013. The experiment was comprised of four inoculums (no, lentil, peas and chickpeas) and two NPK levels (recommended 120:90:60 kg·ha-1 & 20% less of recommended). It was observed that inoculation of wheat by rhizobia significantly increased tillers per plant by a maximum of 42%, plant height by 13%, grain per spike by 16%, 100 grain weight by 10%, biological and grain yield by 10% over un-inoculated (control) treatment. Among inoculums, peas inoculum was found to be the most efficient for all traits except plant height where chickpea inoculum performed better. Similarly recommended NPK significantly increased tillers per plant by 33%, plant height by 19%, grain per spike by 9%, 100 grain weight by 10%, biological yield by 8% and grain yield by 10% compared with 20% less of recommended NPK. Interactive effect of inoculum x NPK was significant for tillers per plant, grain per spike, grain yield and non-significant for plant height, 100 grain weight and biological yield. However, it was evident from the results that inoculation improved all traits both under recommended and 20% less of recommended NPK. The persistent good performance of peas and lentil rhizobial inoculation in wheat growth exhibited that this could be used as a plant growth promoting rhizobacteria for wheat and other cereal crops in prevailing soil and climatic conditions.展开更多
To improve the process of “Attiéké” production by the standardization of the traditional inoculums, the effects of inoculums amount (6%, 8%, 10% and 12%) and fermentation time (6, 12 and 18 h) were studied...To improve the process of “Attiéké” production by the standardization of the traditional inoculums, the effects of inoculums amount (6%, 8%, 10% and 12%) and fermentation time (6, 12 and 18 h) were studied and analyzed for their microbiological and sensory qualities using standard analytical procedures. The microbial analysis showed that Lactobacillus species (9.14 Log cfu/g) as the most important popular microorganism in the paste, followed by enterococci (7.64 Log ufc/g) species, yeasts and moulds (7.30 Log cfu/g) respectively. Sensory analysis revealed significant effects (p < 0.05), of inoculums quantity and fermentation time on the “Attiéké” quality. “Attiéké” obtained with 10% inoculums and after 12 hours of fermentation was identified as the optical inoculums amount and time for “Attiéké” production. The importance of traditional starter inoculums in “Attiéké” processing could be necessary to standardization at small and industrial scale the process of production.展开更多
In 2010, Brazil endorsed a new national policy of solid wastes, which has a very ambitious target to close up all the dumping areas and to manage adequately all the municipal solid waste, disposing in landfills only t...In 2010, Brazil endorsed a new national policy of solid wastes, which has a very ambitious target to close up all the dumping areas and to manage adequately all the municipal solid waste, disposing in landfills only the refuse of treated municipal solid waste after 2019. Food waste is the largest representative of organic waste produced in Brazilian municipalities, which can produce both fertilizer and biogas through anaerobic digestion （AD） process, reducing in both cases greenhouse gases emissions. AD assays of some samples of restaurant food wastes were performed in batch laboratory, 500 mL reactors for determination of optimized start-up conditions. The tests were conducted in triplicate, using anaerobic sludge from a poultry slaughterhouse wastewater treatment as inoculum to start up the anaerobic biodigestors. The effect of three different substrates to inoculum mixing ratios 10：90 （S1）, 20：80 （S2） and 30：70 （S3） was evaluated. The food waste used as substrate was previously homogenized and submitted to aerobic mechanical mixing for four days before it was fed in the reactors in anaerobic conditions at the mentioned proportions with the inoculum. The reactors were maintained at 35 ℃ and under mechanical mixing at 60 rpm. The methane production was monitored until its stabilization. Results showed that S1 presented the best performance during the initial 12 d, when it totalized 526 NmLCH4/gVSsubstrate.展开更多
Two are the critical factors in the commercial production of ornamental plants： the substrate and the fertilization method, since the ＂sustainable floriculture＂ discourages the use of synthetic chemical fertilizers...Two are the critical factors in the commercial production of ornamental plants： the substrate and the fertilization method, since the ＂sustainable floriculture＂ discourages the use of synthetic chemical fertilizers and peat-based substrates. The aim of this paper was to test a peat/compost based substrate and a guano ＋ mycorrhizal inoculum fertilizer mixture, in order to obtain a sustainable quality yield of geranium plants. Geranium cuttings （Pelargonium zonale cv. real polaris and Pelargonium grandiflorum cv. lotus） were grown for two months in a glasshouse. Peat, in the growing substrate, was replaced with high quality compost （20% and 40% as reduction percentages）. The fertilizer mixture was made up of guano （3 g/L） and mycorrhizal inoculum （7.5 L/m^3）. Plants of Pelargonium cv. Real polaris, grown on a substrate made up of peat and 20% of high quality compost, presented the better trade features, so to satisfy the ＂sustainable floriculture＂ need.展开更多
Brown seaweed, Sargassum spp. is also used as an alternative feed ingredient for aquaculture in the form of seaweed meal. Nutritional values seaweed meal with bioprocess technology using microbes such as Lactobacillus...Brown seaweed, Sargassum spp. is also used as an alternative feed ingredient for aquaculture in the form of seaweed meal. Nutritional values seaweed meal with bioprocess technology using microbes such as Lactobacillus, Bacillus or Streptococcus, are improved with different inoculum sizes. Purple non-sulphur bacterium, Afifella marina could be one of the alternative and potential candidates. This study aims to determine the optimum inoculum level and period for the improvement of nutritional values in Sargassum polycystum meal. Sargassum polycystum was collected from the Sepanggar Bay. Dried seaweed was grinded into fine particles (A. marina were used with 20 g Sargassum polycystum powder in one liter bottle with autoclaved 112 synthetic media. Bottles were incubated at 2500 lux light intensity at 30˚C ± 1˚C for 8 days. Every two days destructive samples were taken for the determination of nutritional values of bioprocessed seaweed meal. The measured analytical parameters were crude protein (%), crude ash (%), crude fiber (%) and crude lipids (%). Nutritional value of processed seaweed meal powder with A. marina has improved with the increase of inoculum size. The crude protein percentage of 14.70% + 0.40% was significantly high (p 0.05) were observed among the values of crude protein, ash, lipids and fiber within 6<sup>th</sup> and 8<sup>th</sup> day. A. mariana with 30% (v/v) inoculum sizes on 6<sup>th</sup> of day has capability in improving the nutritional values of Sargassum polycystum seaweed meal during bioprocessing.展开更多
基金supported by the Dutch Technology Foundation (STW)which is part of the Netherlands Organization for Scientific Research (NWO)+1 种基金which is partly funded by the Dutch Ministry of Economic Affairsproject (11611) was co-sponsored by Agrifirm, Purac, DSM, Den Ouden, Hofmans, the Dutch commodity boards for dairy and horticulture, and Wageningen University
文摘Background： The aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization. Wheat straw and wood chips were treated with Ceriporiopsis subvermispora and Lentinula edodes with various amounts of colonized millet grains（0.5, 1.5 or 3.0 % per g of wet weight of substrate） added to the substrates. Also, wheat straw and wood chips were chopped to either 0.5 or 2 cm.Effectiveness of the fungal treatment after 0, 2, 4, 6, or 8 wk of incubation was determined by changes in chemical composition, in vitro gas production（IVGP） as a measure for rumen degradability, and ergosterol content as a measure of fungal biomass.Results： Incomplete colonization was observed for C. subvermispora treated wheat straw and L. edodes treated wood chips. The different particle sizes and amounts of inoculum tested, had no significant effects on the chemical composition and the IVGP of C. subvermispora treated wood chips. Particle size did influence L.edodes treatment of wheat straw. The L. edodes treatment of 2 cm wheat straw resulted in a more selective delignification and a higher IVGP than the smaller particles. Addition of 1.5 % or 3 % L. edodes inoculum to wheat straw resulted in more selective delignification and a higher IVGP than addition of 0.5 % inoculum.Conclusion： Particle size and amount of inoculum did not have an effect on C. subvermispora treatment of wood chips. At least 1.5 % L. edodes colonized millet grains should be added to 2 cm wheat straw to result in an increased IVGP and acid detergent lignin（ADL） degradation.
基金Supported by International foundation for Science (IFS)National Natural Science Foundation of China.
文摘The effects of inoculum forms (single-spore, multi-spores, or colonized root pieces) and host plants (Nicotiana tabacum L., Sorghum sudanense (Piper) Stapf, and Trifolium repens L.) on the development and inoculum potential (IP) of the arbuscular mycorrhizal fungi (AMF) :Glo-mus macrocarpum Tul & Tul, donuis mosseae (Nicol & Gerd.) Gerdemann & Trappe, Glomus ver-siforme (Karsten) Berch, and Sclerocystis sinu/osa Gerdemann & Bakhi cultured in pots were investigated. The lag phase of treatment with 50 spores or 0.5 g (fresh weight) of colonized root pieces was 4 weeks, much shorter than that of the treatment with 1 spore (8 weeks); the value of IP (VIP) and percentage of root colonization (PRC) of the former were greater than those of the latter. Only on the early stages of colonization was there difference between the 50 spores and the 0.5 g (fresh weight) of colonized root piece inoculation treatments. The EP per plant inoculated with 0.5 g (fresh weight) of colonized root pieces of AMF was greater than that of the other two treatments except G. versiforme on Nicotiana tabacum, while the PRC of the plants inoculated with 50 spores and 0.5 g (fresh weight) of colonized root pieces of AMF was higher than that of the 1 spore inoculation after 10 weeks. Trie VIP of AMF on Trifolium repens was significantly higher than that on the other two hosts. The VIP of G. mosseae, G. versiforme, and S. sinuosa was respectively greater than that of G. macrocarpum. This suggested that different species of AMF produced different VIP of the inoculum . Nicotiana tabacum was much better than the other host plants which used to be inoculated with single spore, and to produce inocula of AMF.
基金Supported by National Natural Science Foundation of China(31301264)National Science and Technology Project for Food Production(Hunan)(2011BAD16B01,2012BAD04B10,2013BAD07B11)+3 种基金High-yield and Hich-tech Research and Demonstration of Two-line Hybrid Rice(2012BAD07B02)Integration of Water and Fertilizer Optimized Management Technology Research in the High-yield Rice Area in the Yangtze River(2013BAD07B14/4)Demonstration and Application of Spectrum Detection Technology System of Rice Production in Double-Cropping Ricein Hunan Province(201303109-3)China Special Fund for Meteorological Research in the Public Interest(GYHY201206020)~~
文摘The research designed four application quantities of 0, 2 250, 4 500 and 6 750 g/hm2 based on Liangyou No. 959 to explore the effects of microbial inocu- lure fertilizer on growth and yield of super hybrid rice. The results indicate that the treatment with traditional fertilizers and 4 500 g/hm2 HYT microbial inoculum fertilizer performed the best, where average number of tiller was 2.4 more than that of the control; the number of effective ear was 11.05% higher; yield increased by 10.3%. These have demonstrated that microbial inoculum fertilizer is of great value in pro- motion on super hybrid rice production.
基金supported by the Forest Growers Levy Trust(QT-10353)the Ministry for Primary Industries and the Sustainable Food and Fibre Futures fund(SFFF22023)the Strategic Science Investment Fund(CO4X1703)for funding this research.
文摘Invasive pests and pathogens cause immense damage globally,costing an estimated US$248 billion to the agricultural industry alone.Vehicles,such as farming and timber harvesting machinery and transportation trucks,can facilitate the rapid spread of biological invaders over distances far greater and more quickly than their natural dispersal ability.Understanding how frequent trips by these vehicles increase the spread of invasive agricultural and forestry pests can help inform effective biosecurity procedures before,during,or after an incursion.We used a case study of timber transport trucks in Aotearoa New Zealand to examine whether and how vehicles facilitate the spread of soil-borne pathogens between commercial forest plantations.Our results show that long-distance dispersal associated with truck movement facilitated the introduction of oomycete-like pathogens in 97% of forest sites within only one year,with pathogen loads within infected sites predicted at 84%of the sites’carrying capacity.Implementing preventative management strategies to reduce the transportation of infected soil by logging trucks,however,can reduce the spread by up to 50% after one year and reduce the pathogen load within infested sites by more than three times.Mitigating other human-assisted dispersal pathways can also help reduce spread.Reducing movement of forest visitors not involved in forestry activities,for instance,by closing forest sites to the public,can help to further reduce spread in addition to management related to harvesting activities.These results highlight the benefits of preventative management strategies in reducing the spread rate of novel soil pathogens through a high-intensity commercial forestry network but show that pest spread is still likely even with significant investment.
基金Supported by the International Science and Technology Cooperation Program of China(2011DFR31230)Major Science and Technology Project of Shanxi Province,China(20121101009)Key Project of Shanxi Academy of Agricultural Sciences,China(2013zd12)
文摘Through a pot experiment, effects of various microbial inoculums on soil microbial diversity and enzyme activity in a typical reclaimed mining area in Shanxi Province were discussed based on quantitative analysis of PLFA, soil urease, phosphatase and sucrase activity. The results showed that the application of microbial inoculums increased microorganism quantity in rhizosphere of rape by 2.3% -66.4%, and quantities of bac- teria, gram-positive bacteria, gram-negative bacteria, fungi and actinomycetes in the treatments with microbial inoculums were significantly higher than the contrast （P 〈0.05）, while there was no obvious change in protist quantity. In comparison with the contrast, the application of various mi- crobial inoculums also improved soil urease, sucrase and phosphatase activity by 4.2% - 61.4%, 18.0% - 32.5% and 64.2% - 199.0% respec- tively. It indicated that the application of microbial inoculums can improve soil microbial diversity and enzyme activity, so it is an effective way to sl^eed UP ecoloQical restoration of soil.
文摘Lactate is a key intermediate during anaerobic digestion of carbohydrates;however, it fails to receive significant consideration in biogas plants. We examined the influence of lactic acid on biogas production. Two commonly used feeds, fresh maize and maize silage, were selected as substrates due to their difference in lactic acid contents. Additionally, inocula from an agriculture-based biogas plant, a waste water treatment plant and a standardised laboratory reactor were selected to investigate the impact of starter culture on the process. Experiments demonstrated increased total biogas yield of up to 45% in the lactate-rich maize silage over the lactate-devoid fresh maize, but only in cases where the starting inocula had been previously exposed to lactic acid. Our findings suggest lactic acid is a significant intermediate in biogas production and merits consideration. Additionally, the ability of the starter inoculum to utilize lactic acid is an important factor in process optimization and enhanced biogas production.
基金Supported by Fund Project for Transformation of Agricultural Scientific and Technological Achievements in Hebei Province in 2022Agricultural Products Quality and Safety Technology Innovation Center of Hebei Province+2 种基金Tangshan Talent Project(A202110026)Tangshan Science and Technology Planning Project(23150210A)High-level Talent Fund of Hebei Province(C20231087).
文摘[Objectives]This study was conducted to understand the effects of microbial inocula on the biological yield and nutritional components of corn.[Methods]Silage corn varieties suitable for planting in Hebei Province were selected,and set with an experimental group and a control check group each.Meanwhile,at the late stage of milk ripening of silage corn growth cycle,the nutritional components and indexes of silage corn were determined and analyzed by a near-infrared analyzer and the NIRS technique.Meanwhile,the biological yield of silage corn was determined at the maturation stage of its growth period,aiming at comprehensively evaluating the regulation effects of the microbial inoculum tested on silage corn from the aspects of basic nutritional components,mineral element contents,energy and related indexes of corn,combined with agronomic characters and biological yield.[Results]The microbial inoculum improved the biological yield and nutritional indexes of silage corn,and had a positive regulation effect on the growth of silage corn.[Conclusions]The results of this study provide a theoretical basis for popularizing the planting methods and cultivation methods of silage corn with high yield and high quality.
基金supported by the Steps towards sustainable forest management with the local communities in Tigraynorthern Ethiopia at Mekelle University funded by NORAD/NORHED(ETH 13/0018)program+1 种基金Mekelle UniversityNORADⅢproject
文摘Poor land use management and practice inhibit the growth and establishment of tree seedlings in dryland areas.We assessed arbuscular mycorrhizal fungi(AM)status of Faidherbia albida(Del.)A.Chev.trees grown on different land uses.We quantified the growth and nutrient uptake of F.albida seedlings inoculated with AM from different sources.These efforts were based on soil and fine root samples from the rhizosphere soils of F.albida trees.AM root colonization was determined using the gridline intersect method.Spores were extracted by the wet sieving and decanting method and identified to genus level.The seedling experiment had a completely randomized onefactorial design with four treatments and five replications.Faidherbida albida seedlings were grown in a greenhouse.All in situ F.albida trees were colonized by AM fungi.AM root colonization of F.albida trees was significantly higher(P<0.0086)in area exclosures than on lands used for grazing or cultivation.Spore abundance was significantly higher(P<0.0014)in area exclosures followed by cultivated land and grazing land.Glomus was the dominant genus in all land-uses.AM-inoculated F.albida seedlings grew better(P<0.05)than non-inoculated controls.Seedlings inoculated with AM from area exclosure had significantly(P<0.05)higher growth and nutrient uptake than those inoculated with AM from grazing and cultivated land.This emphasizes the importance of the native soil AM potential for better establishment of seedlings to achieve optimum plant growth improvement and assist in rehabilitation of degraded arid lands.
文摘An experiment was conducted in pots under natural condition with two factor factorial completely randomized design (CRD) (CRD) to investigate the integrated effect of rhizobia inoculums and inorganic fertilizers on growth and yield of wheat crop at The University of Agriculture Peshawar, during 2012-2013. The experiment was comprised of four inoculums (no, lentil, peas and chickpeas) and two NPK levels (recommended 120:90:60 kg·ha-1 & 20% less of recommended). It was observed that inoculation of wheat by rhizobia significantly increased tillers per plant by a maximum of 42%, plant height by 13%, grain per spike by 16%, 100 grain weight by 10%, biological and grain yield by 10% over un-inoculated (control) treatment. Among inoculums, peas inoculum was found to be the most efficient for all traits except plant height where chickpea inoculum performed better. Similarly recommended NPK significantly increased tillers per plant by 33%, plant height by 19%, grain per spike by 9%, 100 grain weight by 10%, biological yield by 8% and grain yield by 10% compared with 20% less of recommended NPK. Interactive effect of inoculum x NPK was significant for tillers per plant, grain per spike, grain yield and non-significant for plant height, 100 grain weight and biological yield. However, it was evident from the results that inoculation improved all traits both under recommended and 20% less of recommended NPK. The persistent good performance of peas and lentil rhizobial inoculation in wheat growth exhibited that this could be used as a plant growth promoting rhizobacteria for wheat and other cereal crops in prevailing soil and climatic conditions.
文摘To improve the process of “Attiéké” production by the standardization of the traditional inoculums, the effects of inoculums amount (6%, 8%, 10% and 12%) and fermentation time (6, 12 and 18 h) were studied and analyzed for their microbiological and sensory qualities using standard analytical procedures. The microbial analysis showed that Lactobacillus species (9.14 Log cfu/g) as the most important popular microorganism in the paste, followed by enterococci (7.64 Log ufc/g) species, yeasts and moulds (7.30 Log cfu/g) respectively. Sensory analysis revealed significant effects (p < 0.05), of inoculums quantity and fermentation time on the “Attiéké” quality. “Attiéké” obtained with 10% inoculums and after 12 hours of fermentation was identified as the optical inoculums amount and time for “Attiéké” production. The importance of traditional starter inoculums in “Attiéké” processing could be necessary to standardization at small and industrial scale the process of production.
文摘In 2010, Brazil endorsed a new national policy of solid wastes, which has a very ambitious target to close up all the dumping areas and to manage adequately all the municipal solid waste, disposing in landfills only the refuse of treated municipal solid waste after 2019. Food waste is the largest representative of organic waste produced in Brazilian municipalities, which can produce both fertilizer and biogas through anaerobic digestion （AD） process, reducing in both cases greenhouse gases emissions. AD assays of some samples of restaurant food wastes were performed in batch laboratory, 500 mL reactors for determination of optimized start-up conditions. The tests were conducted in triplicate, using anaerobic sludge from a poultry slaughterhouse wastewater treatment as inoculum to start up the anaerobic biodigestors. The effect of three different substrates to inoculum mixing ratios 10：90 （S1）, 20：80 （S2） and 30：70 （S3） was evaluated. The food waste used as substrate was previously homogenized and submitted to aerobic mechanical mixing for four days before it was fed in the reactors in anaerobic conditions at the mentioned proportions with the inoculum. The reactors were maintained at 35 ℃ and under mechanical mixing at 60 rpm. The methane production was monitored until its stabilization. Results showed that S1 presented the best performance during the initial 12 d, when it totalized 526 NmLCH4/gVSsubstrate.
文摘Two are the critical factors in the commercial production of ornamental plants： the substrate and the fertilization method, since the ＂sustainable floriculture＂ discourages the use of synthetic chemical fertilizers and peat-based substrates. The aim of this paper was to test a peat/compost based substrate and a guano ＋ mycorrhizal inoculum fertilizer mixture, in order to obtain a sustainable quality yield of geranium plants. Geranium cuttings （Pelargonium zonale cv. real polaris and Pelargonium grandiflorum cv. lotus） were grown for two months in a glasshouse. Peat, in the growing substrate, was replaced with high quality compost （20% and 40% as reduction percentages）. The fertilizer mixture was made up of guano （3 g/L） and mycorrhizal inoculum （7.5 L/m^3）. Plants of Pelargonium cv. Real polaris, grown on a substrate made up of peat and 20% of high quality compost, presented the better trade features, so to satisfy the ＂sustainable floriculture＂ need.
文摘Brown seaweed, Sargassum spp. is also used as an alternative feed ingredient for aquaculture in the form of seaweed meal. Nutritional values seaweed meal with bioprocess technology using microbes such as Lactobacillus, Bacillus or Streptococcus, are improved with different inoculum sizes. Purple non-sulphur bacterium, Afifella marina could be one of the alternative and potential candidates. This study aims to determine the optimum inoculum level and period for the improvement of nutritional values in Sargassum polycystum meal. Sargassum polycystum was collected from the Sepanggar Bay. Dried seaweed was grinded into fine particles (A. marina were used with 20 g Sargassum polycystum powder in one liter bottle with autoclaved 112 synthetic media. Bottles were incubated at 2500 lux light intensity at 30˚C ± 1˚C for 8 days. Every two days destructive samples were taken for the determination of nutritional values of bioprocessed seaweed meal. The measured analytical parameters were crude protein (%), crude ash (%), crude fiber (%) and crude lipids (%). Nutritional value of processed seaweed meal powder with A. marina has improved with the increase of inoculum size. The crude protein percentage of 14.70% + 0.40% was significantly high (p 0.05) were observed among the values of crude protein, ash, lipids and fiber within 6<sup>th</sup> and 8<sup>th</sup> day. A. mariana with 30% (v/v) inoculum sizes on 6<sup>th</sup> of day has capability in improving the nutritional values of Sargassum polycystum seaweed meal during bioprocessing.
