Licochalcone A(LCA)is a characteristic compound in licorice Glycyrrhiza inflata and is widely utilized in pharmaceutical and cosmetic industries.However,the biosynthetic pathway and regulatory mechanisms of LCA remain...Licochalcone A(LCA)is a characteristic compound in licorice Glycyrrhiza inflata and is widely utilized in pharmaceutical and cosmetic industries.However,the biosynthetic pathway and regulatory mechanisms of LCA remain poorly understood.In this study,we first found the accumulation of LCA is induced by methyl jasmonate(MeJA).Given that MYB transcriptional factors are well-documented as key regulators of flavonoid biosynthesis,we identified a total of 147 GiR2R3-MYB genes in G.inflata,which were classified into 28 subgroups.The chromosome distributions,sequence characteristics,gene structures,duplication events and cis-acting elements were also investigated.Through integrated analysis of GiR2R3-MYBs expression patterns across different tissues and under MeJA treatment,along with phylogenetic relationship,we identified GiMYB76—a MeJA-inducible MYB transcription factor—as a potential regulator of LCA accumulation.Functional validation showed that transgenic hairy roots overexpressing GiMYB76 exhibited a significant increase in LCA content.DAP-seq analysis of GiMYB76 revealed potential target genes involved in flavonoid biosynthesis regulation.Subsequent promoter activity assay verified that GiMYB76 can bind to the promoter and activate the expression of GiCHS4.Consistently,overexpression of GiCHS4 in G.inflata hairy roots also significantly enhanced LCA production.This study not only clarifies that GiMYB76 transcriptionally activated GiCHS4 to promote LCA biosynthesis but also provides valuable insights for basic research on licorice and the development of related industries.展开更多
Stomatal conductance was found to change from steady-state to a slate of oscillations during daytime when vapour pressure deficit (VPD) increased to a value of 1 kPa in Glycyrrhiza inflata Batalin grown under the cond...Stomatal conductance was found to change from steady-state to a slate of oscillations during daytime when vapour pressure deficit (VPD) increased to a value of 1 kPa in Glycyrrhiza inflata Batalin grown under the conditions of arid desert in north-west China. The injected metabolic inhibitors (NaN3 or carbonyl cyanide-m-chlorophenyl-hydrazone (CCCP)) slightly reduced the stomatal conductance but did not significantly decrease the intensity of stomatal oscillations (amplitude/average). The oscillation intensity was found to he significantly correlated with VPD and root resistance, but not with the respiration rate. There might exist a minimum threshold of VPD (0.8 kPa) and root resistance (1/4 relative value) that induced stomatal oscillations. These results suggested that stomatal oscillations induced by atmospheric drought stress and root resistance were mainly a type of hydropassive movement.展开更多
Continuing the previous report(1),seventeen compounds were isolated from the roots and rhizoma of Glycyrrhiza inflata Bat. On the basis of spectroscopic methods five of them were identified as sucrose(1),4’,5,7-trihy...Continuing the previous report(1),seventeen compounds were isolated from the roots and rhizoma of Glycyrrhiza inflata Bat. On the basis of spectroscopic methods five of them were identified as sucrose(1),4’,5,7-trihydroxy-8-prenyl-flavonc(2),liquiritigenin(3),formononetin(4),and glabrone(5)(1),(4)and(5)were obtained from this plant for the first time. Their antioxidant activities were observed in three oxidizing systems in vitro.(2),(3)and(5)showed remarkable activities in scavenging(5 exhibited good activity in inhibiting hemolysis induced by H_2O_2(3)and(5)displayed higher inhibitory action on (HpD + hv)-induced hemolysis than som typical antioxidants.展开更多
Seven triterpenoid saponins were isolated from the 10% EtOH extract of the root of Glycyrrhiza inflata Bat. On the basis of chemical and spectroscopic methods,two of them were identified as glycyrrhetic acid-3-O-β-...Seven triterpenoid saponins were isolated from the 10% EtOH extract of the root of Glycyrrhiza inflata Bat. On the basis of chemical and spectroscopic methods,two of them were identified as glycyrrhetic acid-3-O-β-D-6”-ethyl-glucuronopyranosyl-(1→2)-β-D-6’-n-butyl-glucuro-nopyranoside(1),and glycyrrhetic acid-3-O-β-D-6”-n-butyl-glucuronopyranosyl-(1→2)-β-D-6’-n-ethyl-glucuronopyranoside(2). Both are new compounds and named inflasaponins Ⅱ and Ⅵ respec-tively.展开更多
Histone deacetylases(HDACs)play a crucial role in regulating plant growth,stress responses,and specialized metabolism.Licorice,utilized as both food and herbal medicine for millennia,includes Glycyrrhiza inflata as on...Histone deacetylases(HDACs)play a crucial role in regulating plant growth,stress responses,and specialized metabolism.Licorice,utilized as both food and herbal medicine for millennia,includes Glycyrrhiza inflata as one of its primary medicinal species used globally.This study investigated the regulatory function of HDAC-mediated histone deacetylation in flavonoid biosynthesis in licorice.The research identified nineteen HDACs in the G.inflata genome.Abiotic stresses and plant hormones were found to influence flavonoid compound accumulation,correlating with altered expression patterns of HDAC genes and global histone H3 acetylation(H3ac)levels.Notably,several HDAC inhibitors enhanced flavonoid accumulation in G.inflata.Subsequent RNA-seq analysis revealed that the HDAC inhibitor suberoylanilide hydroxamic acid(SAHA)activated the expression of multiple genes related to flavonoid biosynthesis.ChIPqPCR demonstrated that SAHA treatment increased the H3ac levels of flavonoid synthesis-related genes.Furthermore,overexpression of GiHDA2b,an HDAC member,decreased,while RNAi of GiHDA2b increased,the levels of expression and H3K18 acetylation of licochalcone A(LCA)biosynthetic genes indicating its negative role in flavonoid biosynthesis.This research provides valuable insights into the regulatory roles of GiHDACs and histone deacetylation in flavonoid biosynthesis in licorice,potentially contributing to improved bioactive compound production in medicinal plants.展开更多
基金supported by the Guangdong Basic and Applied Basic Research Foundation (2025A1515012679)Open Fund of Shanghai Key Laboratory of Plant Functional Genomics and Resources (PFGR202502)
文摘Licochalcone A(LCA)is a characteristic compound in licorice Glycyrrhiza inflata and is widely utilized in pharmaceutical and cosmetic industries.However,the biosynthetic pathway and regulatory mechanisms of LCA remain poorly understood.In this study,we first found the accumulation of LCA is induced by methyl jasmonate(MeJA).Given that MYB transcriptional factors are well-documented as key regulators of flavonoid biosynthesis,we identified a total of 147 GiR2R3-MYB genes in G.inflata,which were classified into 28 subgroups.The chromosome distributions,sequence characteristics,gene structures,duplication events and cis-acting elements were also investigated.Through integrated analysis of GiR2R3-MYBs expression patterns across different tissues and under MeJA treatment,along with phylogenetic relationship,we identified GiMYB76—a MeJA-inducible MYB transcription factor—as a potential regulator of LCA accumulation.Functional validation showed that transgenic hairy roots overexpressing GiMYB76 exhibited a significant increase in LCA content.DAP-seq analysis of GiMYB76 revealed potential target genes involved in flavonoid biosynthesis regulation.Subsequent promoter activity assay verified that GiMYB76 can bind to the promoter and activate the expression of GiCHS4.Consistently,overexpression of GiCHS4 in G.inflata hairy roots also significantly enhanced LCA production.This study not only clarifies that GiMYB76 transcriptionally activated GiCHS4 to promote LCA biosynthesis but also provides valuable insights for basic research on licorice and the development of related industries.
文摘Stomatal conductance was found to change from steady-state to a slate of oscillations during daytime when vapour pressure deficit (VPD) increased to a value of 1 kPa in Glycyrrhiza inflata Batalin grown under the conditions of arid desert in north-west China. The injected metabolic inhibitors (NaN3 or carbonyl cyanide-m-chlorophenyl-hydrazone (CCCP)) slightly reduced the stomatal conductance but did not significantly decrease the intensity of stomatal oscillations (amplitude/average). The oscillation intensity was found to he significantly correlated with VPD and root resistance, but not with the respiration rate. There might exist a minimum threshold of VPD (0.8 kPa) and root resistance (1/4 relative value) that induced stomatal oscillations. These results suggested that stomatal oscillations induced by atmospheric drought stress and root resistance were mainly a type of hydropassive movement.
文摘Continuing the previous report(1),seventeen compounds were isolated from the roots and rhizoma of Glycyrrhiza inflata Bat. On the basis of spectroscopic methods five of them were identified as sucrose(1),4’,5,7-trihydroxy-8-prenyl-flavonc(2),liquiritigenin(3),formononetin(4),and glabrone(5)(1),(4)and(5)were obtained from this plant for the first time. Their antioxidant activities were observed in three oxidizing systems in vitro.(2),(3)and(5)showed remarkable activities in scavenging(5 exhibited good activity in inhibiting hemolysis induced by H_2O_2(3)and(5)displayed higher inhibitory action on (HpD + hv)-induced hemolysis than som typical antioxidants.
文摘Seven triterpenoid saponins were isolated from the 10% EtOH extract of the root of Glycyrrhiza inflata Bat. On the basis of chemical and spectroscopic methods,two of them were identified as glycyrrhetic acid-3-O-β-D-6”-ethyl-glucuronopyranosyl-(1→2)-β-D-6’-n-butyl-glucuro-nopyranoside(1),and glycyrrhetic acid-3-O-β-D-6”-n-butyl-glucuronopyranosyl-(1→2)-β-D-6’-n-ethyl-glucuronopyranoside(2). Both are new compounds and named inflasaponins Ⅱ and Ⅵ respec-tively.
基金funded by the Biological Resources Programme,Chinese Academy of Sciences(KFJ-BRP-007-017)the Guangdong Provincial Key Laboratory of Applied Botany(2023B1212060046)+2 种基金the South China Botanical Garden,Chinese Academy of Sciences(Grant No:QNXM-02)the Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Botanical Garden(PFGR202502)the Crop Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement Open Research Fund(NYGC202202).
文摘Histone deacetylases(HDACs)play a crucial role in regulating plant growth,stress responses,and specialized metabolism.Licorice,utilized as both food and herbal medicine for millennia,includes Glycyrrhiza inflata as one of its primary medicinal species used globally.This study investigated the regulatory function of HDAC-mediated histone deacetylation in flavonoid biosynthesis in licorice.The research identified nineteen HDACs in the G.inflata genome.Abiotic stresses and plant hormones were found to influence flavonoid compound accumulation,correlating with altered expression patterns of HDAC genes and global histone H3 acetylation(H3ac)levels.Notably,several HDAC inhibitors enhanced flavonoid accumulation in G.inflata.Subsequent RNA-seq analysis revealed that the HDAC inhibitor suberoylanilide hydroxamic acid(SAHA)activated the expression of multiple genes related to flavonoid biosynthesis.ChIPqPCR demonstrated that SAHA treatment increased the H3ac levels of flavonoid synthesis-related genes.Furthermore,overexpression of GiHDA2b,an HDAC member,decreased,while RNAi of GiHDA2b increased,the levels of expression and H3K18 acetylation of licochalcone A(LCA)biosynthetic genes indicating its negative role in flavonoid biosynthesis.This research provides valuable insights into the regulatory roles of GiHDACs and histone deacetylation in flavonoid biosynthesis in licorice,potentially contributing to improved bioactive compound production in medicinal plants.
