The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets(LDs)in several regulatory mechanisms in living cells.LDs are dynamic organelles and therefore the...The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets(LDs)in several regulatory mechanisms in living cells.LDs are dynamic organelles and therefore their complete characterization in terms of number,size,spatial positioning and relative distribution in the cell volume can shed light on the roles played by LDs.Until now,fluorescence microscopy and transmission electron microscopy are assessed as the gold standard methods for identifying LDs due to their high sensitivity and specificity.However,such methods generally only provide 2D assays and partial measurements.Furthermore,both can be destructive and with low productivity,thus limiting analysis of large cell numbers in a sample.Here we demonstrate for the first time the capability of 3D visualization and the full LD characterization in high-throughput with a tomographic phase-contrast flow-cytometer,by using ovarian cancer cells and monocyte cell lines as models.A strategy for retrieving significant parameters on spatial correlations and LD 3D positioning inside each cell volume is reported.The information gathered by this new method could allow more in depth understanding and lead to new discoveries on how LDs are correlated to cellular functions.展开更多
To design a more effective blade pitch adjustment mechanism,research was done on changes to the hydrodynamic characteristics of VVPs(Variable Vector Propeller) caused by different rules for changing pitch angle. A mat...To design a more effective blade pitch adjustment mechanism,research was done on changes to the hydrodynamic characteristics of VVPs(Variable Vector Propeller) caused by different rules for changing pitch angle. A mathematical method for predicting the hydrodynamic characteristics of a VVP under unsteady conditions is presented based on the panel method. Mathematical models for evaluation based on potential flow theory and the Green theorem are also presented. The hydrodynamic characteristics are numerically predicted. To avoid gaps between panels,hyperboloidal quadrilateral panels were used. The pressure Kutta condition on the trailing edge of the VVP blade was satisfied by the Newton-Raphson iterative procedure. The influence coefficients of the panels were calculated by Morino's analytical formulations to improve numerical calculation speed,and the method developed by Yanagizawa was used to eliminate the point singularity on derivation calculus while determining the velocities on propeller surfaces. The calculation results show that it's best for the hydrodynamic characteristics of the VVP that pitch angle changes follow the sine rule.展开更多
嵌入式大气数据传感系统(Flush Air Data Sensing System,FADS)通过在飞行器表面特定区域布置测压孔测量来流压力,根据建立的气动模型反推得到飞行参数(攻角、侧滑角、马赫数、静压及动压等)。利用在尖楔前体表面三维区中分布测压孔的方...嵌入式大气数据传感系统(Flush Air Data Sensing System,FADS)通过在飞行器表面特定区域布置测压孔测量来流压力,根据建立的气动模型反推得到飞行参数(攻角、侧滑角、马赫数、静压及动压等)。利用在尖楔前体表面三维区中分布测压孔的方法,根据三维区中的流动特征和压力分布规律,建立压力分布与飞行参数之间的关系。试验结果表明,基于三维区理论的FADS系统得到的马赫数误差在5%左右,精度较高。展开更多
基金funded by the Italian Ministry of University and Research(PRIN 2017-Prot.2017N7R2CJ)Fondazione Cassa di Risparmio in Bologna(Italy)for the financial support to I.K.finalized to the acquisition of EVOS M5000。
文摘The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets(LDs)in several regulatory mechanisms in living cells.LDs are dynamic organelles and therefore their complete characterization in terms of number,size,spatial positioning and relative distribution in the cell volume can shed light on the roles played by LDs.Until now,fluorescence microscopy and transmission electron microscopy are assessed as the gold standard methods for identifying LDs due to their high sensitivity and specificity.However,such methods generally only provide 2D assays and partial measurements.Furthermore,both can be destructive and with low productivity,thus limiting analysis of large cell numbers in a sample.Here we demonstrate for the first time the capability of 3D visualization and the full LD characterization in high-throughput with a tomographic phase-contrast flow-cytometer,by using ovarian cancer cells and monocyte cell lines as models.A strategy for retrieving significant parameters on spatial correlations and LD 3D positioning inside each cell volume is reported.The information gathered by this new method could allow more in depth understanding and lead to new discoveries on how LDs are correlated to cellular functions.
文摘To design a more effective blade pitch adjustment mechanism,research was done on changes to the hydrodynamic characteristics of VVPs(Variable Vector Propeller) caused by different rules for changing pitch angle. A mathematical method for predicting the hydrodynamic characteristics of a VVP under unsteady conditions is presented based on the panel method. Mathematical models for evaluation based on potential flow theory and the Green theorem are also presented. The hydrodynamic characteristics are numerically predicted. To avoid gaps between panels,hyperboloidal quadrilateral panels were used. The pressure Kutta condition on the trailing edge of the VVP blade was satisfied by the Newton-Raphson iterative procedure. The influence coefficients of the panels were calculated by Morino's analytical formulations to improve numerical calculation speed,and the method developed by Yanagizawa was used to eliminate the point singularity on derivation calculus while determining the velocities on propeller surfaces. The calculation results show that it's best for the hydrodynamic characteristics of the VVP that pitch angle changes follow the sine rule.
文摘嵌入式大气数据传感系统(Flush Air Data Sensing System,FADS)通过在飞行器表面特定区域布置测压孔测量来流压力,根据建立的气动模型反推得到飞行参数(攻角、侧滑角、马赫数、静压及动压等)。利用在尖楔前体表面三维区中分布测压孔的方法,根据三维区中的流动特征和压力分布规律,建立压力分布与飞行参数之间的关系。试验结果表明,基于三维区理论的FADS系统得到的马赫数误差在5%左右,精度较高。
